##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937059_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2426084 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.656252627691373 33.0 31.0 34.0 30.0 34.0 2 31.756249577508445 33.0 31.0 34.0 30.0 34.0 3 31.772592375202176 33.0 31.0 34.0 30.0 34.0 4 35.33799283124574 37.0 35.0 37.0 33.0 37.0 5 35.358842068122954 37.0 35.0 37.0 33.0 37.0 6 35.40688451018184 37.0 35.0 37.0 33.0 37.0 7 35.34167324791722 37.0 35.0 37.0 33.0 37.0 8 35.30994928452601 37.0 35.0 37.0 33.0 37.0 9 36.912781667905975 39.0 37.0 39.0 33.0 39.0 10-11 36.77914573444283 39.0 37.0 39.0 33.0 39.0 12-13 36.67016228621927 39.0 37.0 39.0 32.0 39.0 14-15 37.814442739822695 40.0 38.0 41.0 32.5 41.0 16-17 37.74451729618595 40.0 38.0 41.0 32.0 41.0 18-19 37.686932727803324 40.0 37.0 41.0 32.0 41.0 20-21 37.6174244172914 40.0 37.0 41.0 32.0 41.0 22-23 37.47271116746164 40.0 37.0 41.0 32.0 41.0 24-25 37.29604972457672 40.0 37.0 41.0 31.5 41.0 26-27 37.25377357090686 39.5 37.0 41.0 31.5 41.0 28-29 37.33986065610259 40.0 37.0 41.0 31.5 41.0 30-31 37.214284212747785 40.0 37.0 41.0 31.0 41.0 32-33 37.036715958721956 40.0 36.0 41.0 31.0 41.0 34-35 36.83777066251622 39.0 36.0 41.0 30.0 41.0 36-37 36.68924715714707 39.0 36.0 41.0 30.0 41.0 38-39 36.52794730108273 39.0 35.5 41.0 30.0 41.0 40-41 36.32384698963432 39.0 35.0 41.0 30.0 41.0 42-43 36.12308436146481 39.0 35.0 40.5 29.5 41.0 44-45 35.8771868575037 39.0 35.0 40.0 28.5 41.0 46-47 35.554507593306745 39.0 35.0 40.0 27.0 41.0 48-49 35.23095305026537 38.0 35.0 40.0 26.0 41.0 50-51 34.593716664385894 37.5 33.5 39.5 25.0 40.5 52-53 34.905980584349095 38.0 34.5 40.0 26.0 40.5 54-55 35.21241906710567 39.0 35.0 40.0 26.0 41.0 56-57 35.081720583458775 39.0 35.0 40.0 26.0 41.0 58-59 34.83745266033658 38.0 34.0 40.0 26.0 41.0 60-61 34.54414109321854 38.0 34.0 40.0 25.0 41.0 62-63 34.229160243421084 37.5 34.0 40.0 24.0 41.0 64-65 33.91230126409474 37.0 33.5 40.0 23.0 41.0 66-67 33.52903506226495 36.5 33.0 39.5 22.5 41.0 68-69 33.07859311548982 36.0 33.0 39.0 22.0 41.0 70-71 32.72025783113858 35.5 32.0 39.0 21.0 40.5 72-73 32.332609464470316 35.0 32.0 38.0 20.0 40.0 74-75 31.907385729430636 35.0 31.5 37.0 19.0 39.5 76-77 31.46987264249713 35.0 31.0 37.0 18.0 39.0 78-79 31.036623010580016 35.0 31.0 36.0 14.0 39.0 80-81 30.606524753471028 35.0 31.0 36.0 9.5 37.5 82-83 30.185728111639992 34.0 30.0 35.5 7.0 37.0 84-85 29.838005609039094 34.0 30.0 35.0 6.0 37.0 86-87 29.50587448744561 34.0 30.0 35.0 2.0 36.0 88-89 29.1361694401348 34.0 29.0 35.0 2.0 36.0 90-91 28.796510343417623 34.0 29.0 35.0 2.0 35.5 92-93 28.432302632555178 34.0 29.0 35.0 2.0 35.0 94-95 28.030546551562104 34.0 29.0 35.0 2.0 35.0 96-97 27.58671216660264 34.0 27.0 35.0 2.0 35.0 98-99 26.97246261877165 34.0 26.5 35.0 2.0 35.0 100-101 24.743352249963316 31.0 21.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44465438499443266 1101 2 0.39687211958667135 1101 3 0.5152413089844217 1101 4 0.3584051543684694 1101 5 0.3321246210557902 1101 6 0.28521211113638856 1101 7 0.39148256368632417 1101 8 0.3545672845524379 1101 9 0.537216228480581 1101 10-11 0.44050446387869613 1101 12-13 0.49236238292730405 1101 14-15 0.6956038541915177 1101 16-17 0.7600852663163877 1101 18-19 0.7334760688930757 1101 20-21 0.725018018184997 1101 22-23 0.7905893083945088 1101 24-25 0.7748261235937548 1101 26-27 0.8359902328810307 1101 28-29 0.6930581113094547 1101 30-31 0.5982130191622872 1101 32-33 0.6127883294454435 1101 34-35 0.5834088836672535 1101 36-37 0.609969673107976 1101 38-39 0.7762859370496855 1101 40-41 0.7879274563626026 1101 42-43 0.8426199897182443 1101 44-45 0.7512411658044513 1101 46-47 0.7549173561391953 1101 48-49 0.9116416659766244 1101 50-51 0.8539506993586912 1101 52-53 1.0579691888149227 1101 54-55 1.0731421328094513 1101 56-57 0.9902824219391348 1101 58-59 1.0159928111086032 1101 60-61 1.1150381280359696 1101 62-63 0.9851773866520332 1101 64-65 1.0122412018152431 1101 66-67 1.0056454943745265 1101 68-69 1.097641237020781 1101 70-71 1.095810475957542 1101 72-73 1.0421234788985245 1101 74-75 0.9480143380384867 1101 76-77 0.8891893966436832 1101 78-79 1.0243204678839604 1101 80-81 0.9763820620765493 1101 82-83 0.9072448727144327 1101 84-85 1.0321564897625386 1101 86-87 1.0969741423630346 1101 88-89 1.1603755394471271 1101 90-91 1.1210414035211684 1101 92-93 0.9954492722197088 1101 94-95 1.2324604547224496 1101 96-97 1.020356048805791 1101 98-99 1.0411919944065922 1101 100-101 1.160842990913551 1102 1 0.2591143394543849 1102 2 0.32358432629887446 1102 3 0.2675886013317097 1102 4 0.20807152403484253 1102 5 0.18513917407034342 1102 6 0.12143624736052772 1102 7 0.26495449715826425 1102 8 0.1756642056493547 1102 9 0.10112277238712153 1102 10-11 0.30074503411926656 1102 12-13 0.2488399594048829 1102 14-15 0.4716676102552668 1102 16-17 0.3426889689200934 1102 18-19 0.49697770239470884 1102 20-21 0.4368148699818448 1102 22-23 0.3995775273827249 1102 24-25 0.3613878501554879 1102 26-27 0.35383599072678606 1102 28-29 0.48162755987890904 1102 30-31 0.4940599650092281 1102 32-33 0.5145317211888383 1102 34-35 0.385907646166018 1102 36-37 0.2846949478332519 1102 38-39 0.366450277214021 1102 40-41 0.32580291423806074 1102 42-43 0.3816923587906089 1102 44-45 0.384650729214016 1102 46-47 0.4461093173311568 1102 48-49 0.38068730502226344 1102 50-51 0.1761782015861897 1102 52-53 0.42760189844763374 1102 54-55 0.5848142444815636 1102 56-57 0.6438068254635354 1102 58-59 0.4601191352349261 1102 60-61 0.4385427315405721 1102 62-63 0.5588720703467231 1102 64-65 0.6999305395045781 1102 66-67 0.5873809661099969 1102 68-69 0.5990252584048079 1102 70-71 0.704143314290377 1102 72-73 0.6990732858483213 1102 74-75 0.6231969932211392 1102 76-77 0.5850383224926112 1102 78-79 0.6107099342734266 1102 80-81 0.698146243840732 1102 82-83 0.6557618512314107 1102 84-85 0.770355677961728 1102 86-87 0.8918568272457179 1102 88-89 0.9146325437041334 1102 90-91 0.850213762693528 1102 92-93 0.9097933965638312 1102 94-95 0.9230099052719041 1102 96-97 0.7217984802482214 1102 98-99 0.766129129343728 1102 100-101 1.0239308040013668 1103 1 0.21264205544433779 1103 2 0.3279680342324305 1103 3 0.2725787521677816 1103 4 0.29124190350431434 1103 5 0.19002723666233123 1103 6 0.1451757757073011 1103 7 0.1765820438311252 1103 8 0.16178878097333182 1103 9 0.38833269237650114 1103 10-11 0.3472643506536883 1103 12-13 0.1257741028042858 1103 14-15 0.3400278627922546 1103 16-17 0.44438393809995347 1103 18-19 0.4474963051414065 1103 20-21 0.48571444724245794 1103 22-23 0.3508469905479572 1103 24-25 0.6337537521286336 1103 26-27 0.6396475231017575 1103 28-29 0.6293279460989325 1103 30-31 0.7555233128396495 1103 32-33 0.73936143578441 1103 34-35 0.6865168556876071 1103 36-37 0.7191464102681024 1103 38-39 0.672785419916238 1103 40-41 0.7168322901994557 1103 42-43 0.7638165412546769 1103 44-45 0.8013203008307173 1103 46-47 0.7707336104599847 1103 48-49 0.9561390939181678 1103 50-51 0.827121203699889 1103 52-53 0.946579937025362 1103 54-55 0.9231785362824212 1103 56-57 0.9643420555909117 1103 58-59 1.0080835952462124 1103 60-61 1.0192029575180257 1103 62-63 1.0535030310275317 1103 64-65 1.098274856315662 1103 66-67 1.0957596719962552 1103 68-69 1.073420475473739 1103 70-71 1.0546941481493732 1103 72-73 1.135897124144968 1103 74-75 0.9530586346009038 1103 76-77 0.7170324738598701 1103 78-79 0.9061607825188389 1103 80-81 0.903894309973996 1103 82-83 0.92086371814597 1103 84-85 1.0117137282699282 1103 86-87 1.0160096884831695 1103 88-89 1.110127519523882 1103 90-91 1.1543147221570251 1103 92-93 1.0173798810762982 1103 94-95 1.082732358045714 1103 96-97 1.1081338719461513 1103 98-99 1.3214198733429292 1103 100-101 1.3956841765100272 1104 1 0.4528933698907771 1104 2 0.4595917838061361 1104 3 0.5108037162261212 1104 4 0.4346441536389847 1104 5 0.37879897790166694 1104 6 0.39210416473765264 1104 7 0.3476143316729008 1104 8 0.37860089135429575 1104 9 0.5646911545434605 1104 10-11 0.5914011603358205 1104 12-13 0.5758420093427148 1104 14-15 0.6634608456065436 1104 16-17 0.7020670326858678 1104 18-19 0.7585333319798551 1104 20-21 0.7010987578796204 1104 22-23 0.797056892851721 1104 24-25 0.6409035212946108 1104 26-27 0.796568793092618 1104 28-29 0.7274547017571038 1104 30-31 0.8452675727186332 1104 32-33 0.7586912247619821 1104 34-35 0.8943665984622271 1104 36-37 0.7291958398411253 1104 38-39 0.8364227791171217 1104 40-41 0.7838030048269005 1104 42-43 0.9130575034186208 1104 44-45 0.8567437405770661 1104 46-47 0.7830801579517797 1104 48-49 0.7230413145282455 1104 50-51 0.8163318519512544 1104 52-53 0.9784502921759568 1104 54-55 1.0739955989600958 1104 56-57 0.9811444047835707 1104 58-59 1.0459408125603957 1104 60-61 0.987470057372569 1104 62-63 0.8312925165406071 1104 64-65 0.9757480672766334 1104 66-67 0.8990456675832306 1104 68-69 0.948380105127022 1104 70-71 0.9681026165225148 1104 72-73 0.9990685372396726 1104 74-75 0.8202798863949816 1104 76-77 0.811322163634852 1104 78-79 0.9568913954130025 1104 80-81 0.8953382819581783 1104 82-83 0.9559127108756904 1104 84-85 1.0530806049427355 1104 86-87 1.0460896639476438 1104 88-89 1.0198188279689937 1104 90-91 1.1379998298373906 1104 92-93 1.0968075591065158 1104 94-95 1.1222410690024702 1104 96-97 0.9537056968183855 1104 98-99 0.8680457408534785 1104 100-101 1.0786913937639504 1105 1 0.245360625617824 1105 2 0.37721531282133114 1105 3 0.3476985217896029 1105 4 0.28152673493826796 1105 5 0.3356543313792031 1105 6 0.2983942244841984 1105 7 0.3083636701448995 1105 8 0.21720788704391225 1105 9 0.38608685552850375 1105 10-11 0.40917692258429383 1105 12-13 0.4553408045967018 1105 14-15 0.4894049543803476 1105 16-17 0.5496731060202151 1105 18-19 0.5258517005313408 1105 20-21 0.5328027125728454 1105 22-23 0.5089236493634814 1105 24-25 0.5470177684283186 1105 26-27 0.5873667378035208 1105 28-29 0.4090375704703533 1105 30-31 0.34059118854956694 1105 32-33 0.4380637125335838 1105 34-35 0.36116461786043885 1105 36-37 0.476084006165145 1105 38-39 0.5331225582607928 1105 40-41 0.558772526785134 1105 42-43 0.4067458471415293 1105 44-45 0.43031809691949974 1105 46-47 0.5438330655520147 1105 48-49 0.6939950549911629 1105 50-51 0.4719693891337968 1105 52-53 0.5564145936232023 1105 54-55 0.6486159081589591 1105 56-57 0.6649064245664533 1105 58-59 0.649204780327203 1105 60-61 0.7152866035205747 1105 62-63 0.7255237147531304 1105 64-65 0.799339360454411 1105 66-67 0.9156036961554221 1105 68-69 0.9196839010601323 1105 70-71 0.8919235401401977 1105 72-73 0.9883422328637508 1105 74-75 0.9019705394760429 1105 76-77 0.5984685266436109 1105 78-79 0.7272193982973896 1105 80-81 0.8112688710064404 1105 82-83 0.7832638162644479 1105 84-85 0.7641959442957784 1105 86-87 0.9945966447328409 1105 88-89 0.963513764664885 1105 90-91 0.8973622645644781 1105 92-93 0.9460298196270998 1105 94-95 0.9163295516570003 1105 96-97 0.9046196910727495 1105 98-99 0.8422073179322709 1105 100-101 0.9339391193535107 1106 1 0.6066390029299704 1106 2 0.579949637878908 1106 3 0.5804473172579137 1106 4 0.42895738663849414 1106 5 0.5152162544541667 1106 6 0.4529021229981822 1106 7 0.6241018937289695 1106 8 0.3764754598348432 1106 9 0.4821583012754118 1106 10-11 0.6076339529713763 1106 12-13 0.530221563485874 1106 14-15 0.7259227620564346 1106 16-17 0.6969036703740557 1106 18-19 0.7959720618185102 1106 20-21 0.8210302456082701 1106 22-23 0.9332018784917437 1106 24-25 0.8873861871967677 1106 26-27 0.9730476584047096 1106 28-29 0.8686519873941236 1106 30-31 0.7830473579843584 1106 32-33 0.8037977721113307 1106 34-35 0.8538979380826888 1106 36-37 0.8576336655572518 1106 38-39 0.9892429242398038 1106 40-41 0.9930808305957228 1106 42-43 1.0798956892025302 1106 44-45 1.1994297130114049 1106 46-47 0.9880080116838386 1106 48-49 1.0842121033323409 1106 50-51 1.015911678742981 1106 52-53 1.0053744326618812 1106 54-55 1.0508449418006052 1106 56-57 1.2576284843772285 1106 58-59 1.3787734870181154 1106 60-61 1.2889348751842604 1106 62-63 1.249178594088825 1106 64-65 1.2323458746806466 1106 66-67 1.3392349403566115 1106 68-69 1.2246730257703646 1106 70-71 1.3310641439719433 1106 72-73 1.2987148508273165 1106 74-75 1.3599393919267264 1106 76-77 1.3925811886244297 1106 78-79 1.4432252716967398 1106 80-81 1.4481091643558024 1106 82-83 1.5051168929177408 1106 84-85 1.6202500576107077 1106 86-87 1.6003300513631125 1106 88-89 1.6193528744980803 1106 90-91 1.5498935307782027 1106 92-93 1.6810567957413411 1106 94-95 1.686476500026842 1106 96-97 1.6517778056263204 1106 98-99 1.709063802032997 1106 100-101 1.7847400356694934 1107 1 0.294720378511677 1107 2 0.43540549334005973 1107 3 0.3940545139063403 1107 4 0.30870962622600473 1107 5 0.2842719366198381 1107 6 0.33901898099157535 1107 7 0.35129771965333845 1107 8 0.4095929746083229 1107 9 0.33912825267042734 1107 10-11 0.47614570477446705 1107 12-13 0.4427313714153627 1107 14-15 0.45425980954289 1107 16-17 0.5230629808730498 1107 18-19 0.526197504737894 1107 20-21 0.6018502516263737 1107 22-23 0.7425621958635134 1107 24-25 0.5436237046087697 1107 26-27 0.6020341974668355 1107 28-29 0.5449000222454075 1107 30-31 0.7096223577502272 1107 32-33 0.7068643813385833 1107 34-35 0.7575770466533598 1107 36-37 0.8291672572322213 1107 38-39 0.7947015485196545 1107 40-41 0.8711198636102253 1107 42-43 0.8803208657452615 1107 44-45 0.9764219289662321 1107 46-47 0.952592580975768 1107 48-49 0.8960921475194255 1107 50-51 0.814239780501957 1107 52-53 0.7666640873372543 1107 54-55 0.8559601104074162 1107 56-57 0.7811373838898703 1107 58-59 0.9280428853415899 1107 60-61 0.8308667003528996 1107 62-63 0.8821980397468963 1107 64-65 0.8186940308306276 1107 66-67 0.7132094085907212 1107 68-69 0.9164814145581133 1107 70-71 0.8485200189776947 1107 72-73 0.63916737352654 1107 74-75 0.8093232483292745 1107 76-77 0.8370714633523271 1107 78-79 0.8352768686332865 1107 80-81 0.8250754837121654 1107 82-83 0.8784294913278679 1107 84-85 0.9171645349086326 1107 86-87 0.9361274976164751 1107 88-89 0.907931680575313 1107 90-91 0.9210004393473312 1107 92-93 0.9368103399310783 1107 94-95 1.0287589879407406 1107 96-97 1.0167522897404275 1107 98-99 1.206399730183783 1107 100-101 1.2815120205989743 1108 1 0.043634402548889284 1108 2 0.1881690597153245 1108 3 0.1820005355243275 1108 4 0.1916403803156328 1108 5 0.1536702493496236 1108 6 0.06772604641287217 1108 7 0.09307743565656779 1108 8 -0.020930946702861775 1108 9 0.04659160132284512 1108 10-11 0.07942877832388717 1108 12-13 0.0204072645867015 1108 14-15 0.2636692540494039 1108 16-17 0.26201390098226085 1108 18-19 0.38249168932160416 1108 20-21 0.23851428121309937 1108 22-23 0.26721695248735244 1108 24-25 0.2759898094221356 1108 26-27 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-2.1263064014582866 2316 80-81 -1.9121303122005777 2316 82-83 -2.066021697750049 2316 84-85 -1.7930445040855858 2316 86-87 -1.783435838071032 2316 88-89 -2.134498981802345 2316 90-91 -2.101302023808209 2316 92-93 -2.227586666167497 2316 94-95 -2.634123246602318 2316 96-97 -2.624098690960068 2316 98-99 -2.5548313834473753 2316 100-101 -2.294313409967458 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7404.0 3 2294.0 4 2337.0 5 2539.0 6 2656.0 7 3259.0 8 3844.0 9 4717.0 10 5733.0 11 6988.0 12 8880.0 13 10441.0 14 11581.0 15 13787.0 16 16865.0 17 21285.0 18 18750.0 19 13990.0 20 13736.0 21 14340.0 22 15764.0 23 17756.0 24 20340.0 25 23261.0 26 27235.0 27 31683.0 28 37712.0 29 45442.0 30 56080.0 31 69407.0 32 87931.0 33 115296.0 34 155769.0 35 221143.0 36 332719.0 37 482198.0 38 425547.0 39 75333.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.984073176938445 18.59106842438038 14.432342966168699 22.992515432512477 2 15.214930119758158 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22.82111078042717 26.276338615132772 29.197199072627477 21.705351531812585 22-23 22.367767018325445 26.16844558570265 29.225958811578955 22.23782858439295 24-25 22.23994169553156 26.31420152657399 29.36889619940326 22.076960578491196 26-27 22.069042598251233 26.4274581727793 29.3245657050403 22.178933523929167 28-29 21.907109868003964 26.683773368239695 29.34644205493256 22.06267470882378 30-31 22.13743511662563 26.32910781635755 29.244588747712985 22.288868319303837 32-33 22.005392571893054 26.42925218686606 29.26354854819972 22.301806693041165 34-35 22.416485305351973 26.47756291631056 29.037432769163047 22.068519009174427 36-37 22.338240667500823 26.332230289763388 29.122082343907103 22.20744669882869 38-39 22.078491414196087 26.63536495466097 29.10207595510947 22.184067676033468 40-41 22.347446887274085 26.498194945848375 28.98103378405551 22.17332438282203 42-43 22.091612769644932 26.5120553212147 28.793754338467934 22.60257757067243 44-45 22.330120910222746 26.545317930088245 28.693423983352425 22.431137176336584 46-47 22.49273291025219 26.79940234513764 28.373307910803703 22.334556833806467 48-49 22.452382135514597 26.823388378306994 28.25671305856414 22.467516427614267 50-51 22.260429293553997 27.089631315505198 28.21838227411787 22.43155711682293 52-53 22.526490109919113 27.158259438132564 27.851851668580025 22.4633987833683 54-55 22.38969600484304 26.98293368842357 27.907127791866426 22.720242514866964 56-57 22.40074941969668 27.26849263828767 27.815442821384522 22.515315120631126 58-59 22.622351982394118 26.932407396714737 27.87807199737363 22.567168623517514 60-61 22.51313920828009 26.905440385926806 28.251148540123484 22.330271865669626 62-63 22.288498321844887 26.98122837860829 28.339755520382752 22.390517779164067 64-65 22.250886438364155 27.043230893353787 28.0920704152781 22.613812253003953 66-67 22.262239488490245 27.15353912895353 27.830727788595546 22.753493593960673 68-69 22.21631780423287 27.07201767778622 28.10901631433994 22.60264820364097 70-71 22.35067886222522 26.891194241323713 28.039903216702598 22.718223679748466 72-73 22.31948295512756 26.920597710996685 28.02807689049118 22.731842443384572 74-75 22.19491544117723 27.35142342866838 27.775634879223325 22.67802625093106 76-77 22.32485772554272 27.10090006983567 27.80470012414543 22.769542080476178 78-79 22.2839303508853 27.07350311157453 27.800602681959468 22.84196385558071 80-81 22.386561032561353 27.206300933490716 27.678582289964588 22.72855574398334 82-83 22.328064499631274 27.159247075360106 27.880425906098065 22.632262518910554 84-85 22.35640805343217 27.21652724713204 27.616183747536045 22.810880951899744 86-87 22.267460973156066 27.181211514006847 27.828974423171555 22.722353089665532 88-89 22.374535095518144 27.07822359559138 27.600154297749274 22.947087011141203 90-91 22.286385660093767 26.968229095989155 27.780887301908763 22.964497942008315 92-93 22.356198299720027 26.934657322510745 27.809094092911074 22.900050284858153 94-95 22.26892862336441 26.861571179132078 27.87167531453595 22.997824882967567 96-97 22.131519021783415 26.88329817217683 27.9364619645814 23.048720841458355 98-99 21.938541049980305 27.05994825973473 27.753739118376753 23.247771571908217 100-101 21.980086758559015 26.926434742380977 27.7725401299088 23.320938369151207 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 367.0 1 295.0 2 254.5 3 312.5 4 303.0 5 333.0 6 527.0 7 994.5 8 1654.0 9 1998.0 10 1981.0 11 1871.5 12 1933.5 13 2110.5 14 2298.5 15 2535.5 16 2743.0 17 2966.5 18 3175.0 19 3420.5 20 3797.0 21 4477.5 22 5438.5 23 6767.0 24 8268.5 25 10214.5 26 13449.5 27 17234.5 28 20258.5 29 23645.0 30 27956.5 31 33029.5 32 38089.5 33 42423.0 34 47474.5 35 52875.5 36 58876.0 37 64326.0 38 67796.5 39 69871.5 40 72144.5 41 75539.5 42 77751.5 43 80643.0 44 84948.5 45 87700.0 46 89015.5 47 91720.0 48 97554.5 49 97647.5 50 91516.5 51 87882.5 52 85919.5 53 84494.0 54 82149.0 55 76466.5 56 68699.0 57 61509.5 58 56602.5 59 50773.0 60 42505.5 61 34288.0 62 26808.5 63 20471.5 64 15113.5 65 10800.0 66 7680.0 67 5751.0 68 4251.5 69 2994.0 70 2098.5 71 1394.5 72 949.5 73 696.0 74 507.0 75 326.0 76 201.5 77 108.0 78 58.5 79 45.5 80 36.5 81 28.5 82 25.0 83 23.0 84 17.5 85 15.5 86 11.0 87 9.0 88 7.5 89 7.5 90 7.0 91 3.0 92 1.0 93 1.5 94 1.5 95 1.5 96 1.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.061621938894119085 2 0.09752341633677976 3 0.07015420735638173 4 0.1313227406800424 5 0.10737468282219412 6 0.10399475038786786 7 0.11277433097947144 8 0.09707001076632137 9 0.12147147419462805 10-11 0.11046608443895595 12-13 0.09410226521423001 14-15 0.09581284077550488 16-17 0.11048669378306769 18-19 0.09103147294157993 20-21 0.07483252846974796 22-23 0.07662554140746981 24-25 0.0647133405108809 26-27 0.057994694330451874 28-29 0.06978323916237031 30-31 0.051626406999922506 32-33 0.05222407797916313 34-35 0.04684503916599755 36-37 0.05267748354962153 38-39 0.05344002928175612 40-41 0.06766047671886052 42-43 0.06401262281108155 44-45 0.05057533045022349 46-47 0.038683738897746324 48-49 0.04647407097198613 50-51 0.02901795650933768 52-53 0.042372811493748774 54-55 0.047834287683361336 56-57 0.052533218140839305 58-59 0.05935491104182707 60-61 0.05669630565141191 62-63 0.06374470133762887 64-65 0.04412460574324714 66-67 0.06491943395199835 68-69 0.05548035434881892 70-71 0.027431037012733275 72-73 0.02186651410256199 74-75 0.031346812393964925 76-77 0.04556725983106932 78-79 0.06545527689890375 80-81 0.04313535722588336 82-83 0.06232265659391843 84-85 0.0670628057396199 86-87 0.06854667851566558 88-89 0.09063989540345677 90-91 0.06731011786896084 92-93 0.0781094141835155 94-95 0.08559060609607912 96-97 0.0925153457176256 98-99 0.12445982909083116 100-101 0.12046161633315251 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2426084.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.893003795617005 #Duplication Level Percentage of deduplicated Percentage of total 1 80.32157307912526 48.91021854377103 2 11.586313099715895 14.110508151164138 3 3.4370168501846496 6.278708403116805 4 1.4947571298711047 3.6408100633106706 5 0.795584364883355 2.4222760875287843 6 0.4892106091945406 1.7873702089523564 7 0.32703642405491773 1.3939961147896782 8 0.24242972467115065 1.180981931565661 9 0.19004228990120656 1.0415021292253726 >10 1.0106198842081933 11.257710977761027 >50 0.07110689434892827 2.9533902679715434 >100 0.032817791133025395 3.4431670961494314 >500 8.137411133483185E-4 0.3294325004452852 >1k 5.424940755636129E-4 0.6547770317807183 >5k 1.3562351889090323E-4 0.5951504924673957 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8701 0.3586438062325954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5712 0.2354411471325807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3989 0.1644213473235057 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3183 0.13119908461537194 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.1218688223491025E-5 0.0 2 8.243737644698205E-5 0.0 0.0 4.1218688223491025E-5 0.0 3 1.236560646704731E-4 0.0 0.0 4.1218688223491025E-5 0.0 4 1.236560646704731E-4 0.0 0.0 4.1218688223491025E-5 0.0 5 1.236560646704731E-4 0.0 0.0 4.1218688223491025E-5 0.0 6 1.236560646704731E-4 0.0 0.0 4.1218688223491025E-5 0.0 7 1.236560646704731E-4 0.0 0.0 4.1218688223491025E-5 0.0 8 1.236560646704731E-4 0.0 0.0 4.1218688223491025E-5 0.0 9 1.236560646704731E-4 0.0 0.0 4.1218688223491025E-5 0.0 10-11 1.648747528939641E-4 0.0 0.0 4.1218688223491025E-5 0.0 12-13 2.6792147345269167E-4 0.0 0.0 4.1218688223491025E-5 4.1218688223491025E-5 14-15 5.152336027936378E-4 0.0 0.0 4.1218688223491025E-5 4.1218688223491025E-5 16-17 5.564522910171288E-4 0.0 0.0 6.182803233523653E-5 4.1218688223491025E-5 18-19 6.388896674641108E-4 0.0 0.0 1.0304672055872757E-4 4.1218688223491025E-5 20-21 7.007176997993474E-4 0.0 0.0 1.442654087822186E-4 4.1218688223491025E-5 22-23 7.007176997993474E-4 0.0 0.0 2.6792147345269167E-4 4.1218688223491025E-5 24-25 7.419363880228385E-4 0.0 0.0 3.7096819401141924E-4 4.1218688223491025E-5 26-27 7.62545732134584E-4 0.0 0.0 5.152336027936378E-4 1.0304672055872757E-4 28-29 0.0011129045820342577 0.0 0.0 0.0013396073672634584 1.648747528939641E-4 30-31 0.0012365606467047308 0.0 0.0 0.0034417604666615005 1.648747528939641E-4 32-33 0.0012777793349282218 0.0 0.0 0.005667569630730016 1.648747528939641E-4 34-35 0.0015044821201574223 0.0 0.0 0.007481191912563622 2.0609344111745514E-4 36-37 0.001689966217163132 0.0 0.0 0.009315423538508972 2.0609344111745514E-4 38-39 0.001772403593610114 0.0 0.0 0.013169370887405382 2.0609344111745514E-4 40-41 0.001772403593610114 0.0 0.0 0.018074394786000814 2.0609344111745514E-4 42-43 0.0018960596582805872 0.0 0.0 0.022629059834696572 2.2670278522920066E-4 44-45 0.00201971572295106 0.0 0.0 0.02508157178399429 2.473121293409462E-4 46-47 0.0021433717876215333 0.0 0.0 0.028379066841873572 2.473121293409462E-4 48-49 0.0021433717876215333 0.0 0.0 0.03355201221392169 2.473121293409462E-4 50-51 0.0021433717876215333 0.0 0.0 0.03936384725343393 2.473121293409462E-4 52-53 0.0021433717876215333 0.0 0.0 0.04970973799753017 2.473121293409462E-4 54-55 0.002184590475845024 0.0 0.0 0.056737524339635394 2.473121293409462E-4 56-57 0.0022258091640685153 0.0 0.0 0.07780027402183931 2.6792147345269167E-4 58-59 0.0022258091640685153 0.0 0.0 0.11456734391719331 2.8853081756443716E-4 60-61 0.0022258091640685153 0.0 0.0 0.1442654087822186 2.8853081756443716E-4 62-63 0.0022670278522920065 0.0 0.0 0.15968119817780424 2.8853081756443716E-4 64-65 0.0022670278522920065 0.0 0.0 0.1898738873015114 2.8853081756443716E-4 66-67 0.0023082465405154973 0.0 0.0 0.2567512089441256 2.8853081756443716E-4 68-69 0.002328855884627243 0.0 0.0 0.3534502515164355 2.8853081756443716E-4 70-71 0.0023906839169624793 0.0 0.0 0.4334557253582316 3.091401616761827E-4 72-73 0.0024731212934094617 0.0 0.0 0.5286296764662723 3.297495057879282E-4 74-75 0.0025555586698564436 0.0 0.0 0.6424550839954428 3.297495057879282E-4 76-77 0.0025555586698564436 0.0 0.0 0.713310009051624 3.297495057879282E-4 78-79 0.002576168013968189 0.0 0.0 0.8122966888203376 3.297495057879282E-4 80-81 0.0030295735844265903 0.0 0.0 0.9166624074022169 3.297495057879282E-4 82-83 0.003091401616761827 0.0 0.0 0.9909384835809477 3.297495057879282E-4 84-85 0.003194448337320554 0.0 0.0 1.1307934927232528 3.297495057879282E-4 86-87 0.003297495057879282 0.0 0.0 1.3662552491999453 3.7096819401141924E-4 88-89 0.003318104401991028 0.0 0.0 1.6757251603819159 4.121868822349103E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11615 0.0 30.814854 1 GGTATCA 7865 0.0 28.52513 1 GTACATG 10105 0.0 26.200119 1 TACATGG 9970 0.0 25.793152 2 ATCAACG 14045 0.0 25.312109 3 TCAACGC 14070 0.0 25.200617 4 CAACGCA 14140 0.0 25.141012 5 TATCAAC 14190 0.0 25.090061 2 AACGCAG 14615 0.0 24.323908 6 ACATGGG 10350 0.0 23.603222 3 ACGCAGA 16280 0.0 21.602692 7 CGCAGAG 16260 0.0 21.600481 8 CATGGGG 5375 0.0 21.222631 4 GAGTACT 7745 0.0 20.856653 12-13 GTGTAGC 1435 0.0 20.536392 1 AGAGTAC 12780 0.0 20.395367 10-11 AGTACTT 8160 0.0 19.912374 12-13 GCAGAGT 17310 0.0 19.7411 9 GTACTTT 8260 0.0 19.325994 14-15 TAGCCCT 1535 0.0 18.268803 4 >>END_MODULE