FastQCFastQC Report
Thu 26 May 2016
SRR937059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2426084
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101540.41853456022132784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72440.298588177490969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49430.20374397588871612No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41150.16961490203966556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA137250.031.2918471
GGTATCA94400.027.7806361
ATCAACG164850.025.7598363
TCAACGC166850.025.3646094
TATCAAC167750.025.3155542
CAACGCA168050.025.1552225
AACGCAG174250.024.2329146
GTACATG98800.023.6582851
TACATGG99150.022.948552
GCGTATC1302.9390803E-421.927191
ACATGGG100500.021.741343
ACGCAGA196050.021.4656247
GAGTACT98150.021.46204212-13
CGCAGAG195500.021.380248
AGAGTAC150000.020.61419510-11
AGTACTT104200.020.21592512-13
GTACTTT105100.019.83924114-15
GCAGAGT207550.019.521049
GTGTAGC14250.018.6701721
CATGGGG50450.018.0765974
Produced by FastQC (version 0.11.5)