##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937059_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2426084 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.179094375957305 33.0 31.0 34.0 31.0 34.0 2 32.18093190507831 33.0 31.0 34.0 30.0 34.0 3 32.366500912581756 34.0 31.0 34.0 31.0 34.0 4 36.08375060385378 37.0 35.0 37.0 35.0 37.0 5 36.01553120172261 37.0 35.0 37.0 35.0 37.0 6 36.05532825738928 37.0 35.0 37.0 35.0 37.0 7 35.997457631310375 37.0 35.0 37.0 35.0 37.0 8 35.97466493328343 37.0 35.0 37.0 35.0 37.0 9 37.67219766504375 39.0 37.0 39.0 35.0 39.0 10-11 37.602410922292876 39.0 37.0 39.0 35.0 39.0 12-13 37.51073396469372 39.0 37.0 39.0 35.0 39.0 14-15 38.745812799556816 40.0 38.0 41.0 35.0 41.0 16-17 38.6717197343538 40.0 38.0 41.0 34.0 41.0 18-19 38.721182160221986 40.0 38.0 41.0 34.5 41.0 20-21 38.794511030945344 40.0 38.0 41.0 35.0 41.0 22-23 38.72627493524544 40.0 38.0 41.0 34.5 41.0 24-25 38.62911218243062 40.0 38.0 41.0 34.0 41.0 26-27 38.47968165982711 40.0 38.0 41.0 34.0 41.0 28-29 38.311133497438675 40.0 38.0 41.0 34.0 41.0 30-31 38.18069922558328 40.0 38.0 41.0 34.0 41.0 32-33 38.17591765165592 40.0 38.0 41.0 34.0 41.0 34-35 38.09203143831788 40.0 38.0 41.0 33.5 41.0 36-37 37.94179941832187 40.0 37.0 41.0 33.0 41.0 38-39 37.78605419268253 40.0 37.0 41.0 33.0 41.0 40-41 37.618275583203214 40.0 37.0 41.0 33.0 41.0 42-43 37.399028434299886 40.0 37.0 41.0 32.5 41.0 44-45 37.06814994864151 40.0 36.5 41.0 31.0 41.0 46-47 36.79884909178742 40.0 36.0 41.0 31.0 41.0 48-49 36.47686271373951 39.0 36.0 41.0 30.0 41.0 50-51 36.212735008350904 39.0 35.5 41.0 30.0 41.0 52-53 35.96527840750774 39.0 35.0 40.0 29.0 41.0 54-55 35.73097201086195 39.0 35.0 40.0 28.5 41.0 56-57 35.45176589104087 38.0 35.0 40.0 28.0 41.0 58-59 35.126846597232415 38.0 34.0 40.0 27.5 41.0 60-61 34.79598562951654 38.0 34.0 40.0 26.5 41.0 62-63 34.43601149012153 37.0 34.0 40.0 26.0 41.0 64-65 34.036752643354475 37.0 33.5 39.5 26.0 41.0 66-67 33.65408473078426 36.0 33.0 39.0 25.5 40.0 68-69 33.21238691652886 36.0 32.5 39.0 24.5 40.0 70-71 32.738401267227346 35.0 32.0 38.0 23.5 40.0 72-73 32.403560429070055 35.0 32.0 37.5 22.5 39.5 74-75 31.96699908164763 35.0 31.0 37.0 22.0 39.0 76-77 30.57440632723352 33.5 29.5 35.5 20.0 38.0 78-79 31.28378778311056 34.0 31.0 36.0 21.0 38.5 80-81 31.11382561362261 34.0 31.0 36.0 20.0 37.5 82-83 30.787240466529603 34.0 31.0 35.0 19.0 37.0 84-85 30.410765661864964 34.0 31.0 35.0 18.0 36.5 86-87 30.064816799418324 34.0 30.0 35.0 14.5 36.0 88-89 29.713662428835935 34.0 30.0 35.0 8.5 36.0 90-91 29.307860321406842 34.0 29.5 35.0 4.5 35.0 92-93 28.866031843909774 34.0 29.0 35.0 2.0 35.0 94-95 28.411224631958333 34.0 29.0 35.0 2.0 35.0 96-97 27.8055283741206 33.0 28.0 35.0 2.0 35.0 98-99 27.11378872289665 33.0 27.0 35.0 2.0 35.0 100-101 24.16213515278119 29.5 20.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31162080585221474 1101 2 0.17339477954406846 1101 3 0.2154508876569352 1101 4 0.20933230296184036 1101 5 0.24208768277576098 1101 6 0.18721377807994344 1101 7 0.23844741395509317 1101 8 0.1992784536107024 1101 9 0.3052893834536974 1101 10-11 0.335612962367172 1101 12-13 0.25611322020294125 1101 14-15 0.4217031953858452 1101 16-17 0.40579004214201575 1101 18-19 0.3615498450686232 1101 20-21 0.3416612315278016 1101 22-23 0.31278397112014744 1101 24-25 0.24814968403538984 1101 26-27 0.28089419181949893 1101 28-29 0.3101583076881127 1101 30-31 0.2565021744798983 1101 32-33 0.45174469331117706 1101 34-35 0.3487377233708173 1101 36-37 0.41569450164602273 1101 38-39 0.3739129134747188 1101 40-41 0.3705561339190311 1101 42-43 0.4565028159673332 1101 44-45 0.516186771829112 1101 46-47 0.42928545533300166 1101 48-49 0.35089151438328514 1101 50-51 0.45403204978600087 1101 52-53 0.3558072320831869 1101 54-55 0.3829514403035077 1101 56-57 0.47950672068390787 1101 58-59 0.43166116316732683 1101 60-61 0.47006414520357254 1101 62-63 0.531259896273319 1101 64-65 0.4313156925705286 1101 66-67 0.4705901186057204 1101 68-69 0.4370890004688306 1101 70-71 0.34033412759925596 1101 72-73 0.40972466741550306 1101 74-75 0.3884723056735915 1101 76-77 0.2537781375037831 1101 78-79 0.46493730113213516 1101 80-81 0.5662643378806358 1101 82-83 0.5543310747375259 1101 84-85 0.527992815993116 1101 86-87 0.3787423603082196 1101 88-89 0.49015352243279153 1101 90-91 0.5788025652953017 1101 92-93 0.7585273901953293 1101 94-95 0.7676639201072604 1101 96-97 0.8266245476205256 1101 98-99 0.8941863758164956 1101 100-101 0.8769467249010567 1102 1 0.2432018374332472 1102 2 0.06496591111520189 1102 3 0.11439158659763393 1102 4 0.12298243661197716 1102 5 0.1368406775287596 1102 6 0.14036099122715484 1102 7 0.20313803864571867 1102 8 0.22971504404729615 1102 9 0.3049626831270018 1102 10-11 0.3182493350035358 1102 12-13 0.29404906813878284 1102 14-15 0.38927373295639 1102 16-17 0.40155877791075056 1102 18-19 0.31988466340344246 1102 20-21 0.3620681219346906 1102 22-23 0.2945669629031471 1102 24-25 0.3208325867182893 1102 26-27 0.2977944987198029 1102 28-29 0.24221849974830434 1102 30-31 0.22407667205438742 1102 32-33 0.33806683963332773 1102 34-35 0.1914067660398615 1102 36-37 0.28057025652177714 1102 38-39 0.33519100475280794 1102 40-41 0.35421616757906804 1102 42-43 0.2889639584284751 1102 44-45 0.2804516960940333 1102 46-47 0.24515814120569246 1102 48-49 0.25341898691075926 1102 50-51 0.4036518894058432 1102 52-53 0.4979594942354524 1102 54-55 0.38159414894621335 1102 56-57 0.35427357545076177 1102 58-59 0.38038010188626714 1102 60-61 0.37739312253254553 1102 62-63 0.5482463332597618 1102 64-65 0.3347104059652395 1102 66-67 0.36681425482985475 1102 68-69 0.21884625222608634 1102 70-71 0.2619468392119728 1102 72-73 0.3150013726922083 1102 74-75 0.201976909178196 1102 76-77 0.2545572682829125 1102 78-79 0.25365722985206673 1102 80-81 0.325912887529185 1102 82-83 0.36772578813224044 1102 84-85 0.28639396439426434 1102 86-87 0.2783077498736084 1102 88-89 0.43132073360000334 1102 90-91 0.5701865866793234 1102 92-93 0.5353286969966362 1102 94-95 0.5232019856453256 1102 96-97 0.4841238051197827 1102 98-99 0.7697957214258402 1102 100-101 0.9514888494431837 1103 1 0.013924091010487416 1103 2 -0.11923171401898003 1103 3 0.038971613821168205 1103 4 0.1362342653471913 1103 5 0.09743184869395094 1103 6 0.12841611334572178 1103 7 0.13759046939724584 1103 8 0.14445863379088308 1103 9 0.11551705825841196 1103 10-11 0.19939994421306295 1103 12-13 0.21081477976854757 1103 14-15 0.17343096545199188 1103 16-17 0.2868867052703976 1103 18-19 0.20731826454549918 1103 20-21 0.23555408964271862 1103 22-23 0.2935197975810553 1103 24-25 0.25444700855023683 1103 26-27 0.22661008142510752 1103 28-29 0.2997738597961117 1103 30-31 0.3047965186730366 1103 32-33 0.3948988671224569 1103 34-35 0.3347295308883034 1103 36-37 0.3131523144437196 1103 38-39 0.3954656746951528 1103 40-41 0.2668113392754421 1103 42-43 0.3501928352918 1103 44-45 0.43463081254656544 1103 46-47 0.3575602028902267 1103 48-49 0.31893280340644736 1103 50-51 0.19325825484302328 1103 52-53 0.25260658430550365 1103 54-55 0.29361704548572476 1103 56-57 0.2655164237404364 1103 58-59 0.2666883807172056 1103 60-61 0.2460798361210763 1103 62-63 0.1960330379951003 1103 64-65 0.16961697723888136 1103 66-67 0.09863535417361646 1103 68-69 0.22991425018985012 1103 70-71 0.1831911538097657 1103 72-73 0.22722623242461992 1103 74-75 0.2486618078646039 1103 76-77 0.23707901390888964 1103 78-79 0.25104913804457496 1103 80-81 0.28742285289111535 1103 82-83 0.31676881459218365 1103 84-85 0.3440238848262993 1103 86-87 0.0779519626598173 1103 88-89 0.44297815975893684 1103 90-91 0.5374191652789726 1103 92-93 0.44545908775391396 1103 94-95 0.5381792163702919 1103 96-97 0.7298266923634564 1103 98-99 0.7690606307360675 1103 100-101 0.6627221528970288 1104 1 0.23037226572852632 1104 2 0.09969095206281509 1104 3 0.15501542227531928 1104 4 0.14790415762464448 1104 5 0.15973233181251345 1104 6 0.09748836060023791 1104 7 0.19264010684742772 1104 8 0.2707550039544273 1104 9 0.23895044778888064 1104 10-11 0.3045849578968003 1104 12-13 0.30092892541753 1104 14-15 0.34969422590580024 1104 16-17 0.3369892429383441 1104 18-19 0.33476782571380426 1104 20-21 0.2172052398360691 1104 22-23 0.23241972973477232 1104 24-25 0.21594846007509005 1104 26-27 0.13421990684445007 1104 28-29 0.2893678003878293 1104 30-31 0.26766385870101317 1104 32-33 0.37065383779671635 1104 34-35 0.3278085772322541 1104 36-37 0.2752672295394589 1104 38-39 0.27136451745204937 1104 40-41 0.36611990670657946 1104 42-43 0.349600987932682 1104 44-45 0.32670212164242685 1104 46-47 0.3060358155855596 1104 48-49 0.27039424008465573 1104 50-51 0.08682786115380736 1104 52-53 0.05079266027164664 1104 54-55 -0.002955120425546909 1104 56-57 0.08154476191620574 1104 58-59 0.1466301329348667 1104 60-61 0.14218817988843568 1104 62-63 0.29902532424616624 1104 64-65 0.07577527780394178 1104 66-67 0.31678013790304504 1104 68-69 0.26233276836916275 1104 70-71 0.04350945290462249 1104 72-73 0.14319671145795354 1104 74-75 0.1776626447961256 1104 76-77 0.11122212317274105 1104 78-79 0.15091034238239942 1104 80-81 0.3489507259040785 1104 82-83 0.3637501068530078 1104 84-85 0.3635520609181313 1104 86-87 0.37974862385138053 1104 88-89 0.48533659541403296 1104 90-91 0.6392795001479854 1104 92-93 0.6345758598856008 1104 94-95 0.5198898676343653 1104 96-97 0.5105208092591091 1104 98-99 0.40553966768035465 1104 100-101 0.2995368060072927 1105 1 0.12032976335984813 1105 2 0.05544483291143365 1105 3 0.07064767516524029 1105 4 0.06534460358060556 1105 5 0.09571852986145757 1105 6 0.06955910600936477 1105 7 0.12249004813857312 1105 8 0.16693170752741793 1105 9 0.132482856960344 1105 10-11 0.1929923633091235 1105 12-13 0.2427689894767724 1105 14-15 0.32203728257992026 1105 16-17 0.21178711626667734 1105 18-19 0.20501126008761617 1105 20-21 0.08827453082544423 1105 22-23 0.2155096628433597 1105 24-25 0.1755655091396946 1105 26-27 0.10934176117841332 1105 28-29 0.1122827768134087 1105 30-31 0.1402101243518885 1105 32-33 0.14971546441368133 1105 34-35 0.28777502081739215 1105 36-37 0.24367482662717777 1105 38-39 0.358575504815029 1105 40-41 0.17176641628093137 1105 42-43 0.28885971304668345 1105 44-45 0.3269343628335406 1105 46-47 0.3963112134855251 1105 48-49 0.5864148504782918 1105 50-51 0.5136319811174914 1105 52-53 0.5852679604171556 1105 54-55 0.5670750585364814 1105 56-57 0.5494503677278004 1105 58-59 0.5534781168235554 1105 60-61 0.4828361881661678 1105 62-63 0.42229499931339376 1105 64-65 0.5384462448295011 1105 66-67 0.5284449742899042 1105 68-69 0.2708616629407459 1105 70-71 0.09762470732562889 1105 72-73 0.3478159069566189 1105 74-75 0.4509384006003323 1105 76-77 0.4633229847536846 1105 78-79 0.4259531471355551 1105 80-81 0.7379290265958609 1105 82-83 0.8318316905148606 1105 84-85 0.7649078835047014 1105 86-87 0.610075126380007 1105 88-89 0.6609444790895296 1105 90-91 0.7371307858107663 1105 92-93 0.6887123473148336 1105 94-95 0.6819733164961939 1105 96-97 0.9222909679762559 1105 98-99 1.0548397715485969 1105 100-101 1.135245537383799 1106 1 0.3389275840792365 1106 2 0.26781990783422316 1106 3 0.21667703121436688 1106 4 0.2264971223352532 1106 5 0.16714922378822195 1106 6 0.15309161438722185 1106 7 0.22929368259277538 1106 8 0.2794869490339238 1106 9 0.3916500244155685 1106 10-11 0.346790425814568 1106 12-13 0.3117427241760069 1106 14-15 0.5002121810113991 1106 16-17 0.5219120442762843 1106 18-19 0.5074798902624664 1106 20-21 0.4804247142176692 1106 22-23 0.4334858150121761 1106 24-25 0.43361826729537967 1106 26-27 0.46208470748853614 1106 28-29 0.46250434886851366 1106 30-31 0.48360947054424486 1106 32-33 0.4989378704430081 1106 34-35 0.5539564862582864 1106 36-37 0.4698992302065861 1106 38-39 0.6633230976088313 1106 40-41 0.5914788026944606 1106 42-43 0.6249681382976888 1106 44-45 0.7043758292206235 1106 46-47 0.550291964437676 1106 48-49 0.525165543013145 1106 50-51 0.5097520264875683 1106 52-53 0.6199375162134757 1106 54-55 0.5165915646184729 1106 56-57 0.5733818700191762 1106 58-59 0.48130484176806476 1106 60-61 0.5401686905228402 1106 62-63 0.579665016825679 1106 64-65 0.5582783513123246 1106 66-67 0.7261234527893592 1106 68-69 0.6698080715928114 1106 70-71 0.6591456526684567 1106 72-73 0.667020519189883 1106 74-75 0.7550668662558842 1106 76-77 0.7534145459745467 1106 78-79 0.7812893114718769 1106 80-81 0.6683396836369617 1106 82-83 0.8381633260544383 1106 84-85 0.6770353271215157 1106 86-87 0.6583780885329062 1106 88-89 0.8575651435867471 1106 90-91 0.9693202750768144 1106 92-93 0.9669372724702399 1106 94-95 0.9298369662066861 1106 96-97 1.1999848357047362 1106 98-99 1.3234115330171115 1106 100-101 1.3476247176649423 1107 1 0.2208606755752598 1107 2 0.11359283782667973 1107 3 0.06969693698495405 1107 4 0.11774993543469492 1107 5 0.17978094117653143 1107 6 0.1840404926288315 1107 7 0.13564490480228386 1107 8 0.14111523430857176 1107 9 0.1094060108662731 1107 10-11 0.26422445565510344 1107 12-13 0.21729760562373457 1107 14-15 0.23224560146751116 1107 16-17 0.3312001891311098 1107 18-19 0.26087016625261583 1107 20-21 0.25994174672807446 1107 22-23 0.3343329920997178 1107 24-25 0.1980865665607041 1107 26-27 0.29006868987234213 1107 28-29 0.25264336392640274 1107 30-31 0.3265589180224069 1107 32-33 0.3814146479940774 1107 34-35 0.3649041918237401 1107 36-37 0.302931013395046 1107 38-39 0.30835025849877695 1107 40-41 0.2216451435498854 1107 42-43 0.13777830197966523 1107 44-45 0.2565334515545672 1107 46-47 0.23321529544058706 1107 48-49 0.22037957880664294 1107 50-51 0.23697431906131072 1107 52-53 0.1998306601704698 1107 54-55 0.16391328221444468 1107 56-57 0.16875871642425722 1107 58-59 0.1667079960226161 1107 60-61 0.2590844251902027 1107 62-63 0.21074842708195973 1107 64-65 0.33488944751615435 1107 66-67 0.14500958552734744 1107 68-69 0.24360961081170274 1107 70-71 0.2094333044292398 1107 72-73 0.14601332881201756 1107 74-75 0.26536788978229353 1107 76-77 0.216481151130008 1107 78-79 0.5095003369893902 1107 80-81 0.5162751424729741 1107 82-83 0.46852317883471883 1107 84-85 0.43842189045152224 1107 86-87 0.46443669139513233 1107 88-89 0.4840047766205444 1107 90-91 0.6536191592835969 1107 92-93 0.852814403697181 1107 94-95 0.8785482643306892 1107 96-97 1.0894866763584083 1107 98-99 1.0866796715280813 1107 100-101 0.8595754278058614 1108 1 0.06929784180845644 1108 2 -0.07057511189946553 1108 3 -0.010026635416537033 1108 4 0.02969881878175329 1108 5 0.07879018029647966 1108 6 0.06767794532605365 1108 7 0.10526013979776394 1108 8 0.08145779026494182 1108 9 0.17327453435745355 1108 10-11 0.14431822604501576 1108 12-13 0.1419485958569524 1108 14-15 0.15917352949050922 1108 16-17 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582.0 9 1141.0 10 1991.0 11 3128.0 12 5005.0 13 6873.0 14 9049.0 15 12496.0 16 16542.0 17 22693.0 18 20621.0 19 12952.0 20 11243.0 21 11394.0 22 11984.0 23 13057.0 24 15233.0 25 18164.0 26 22028.0 27 27867.0 28 34651.0 29 43869.0 30 55179.0 31 70259.0 32 89371.0 33 117708.0 34 161224.0 35 232182.0 36 360385.0 37 552041.0 38 427911.0 39 36886.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.13305154514225 19.444663167194282 14.884080836080651 22.53820445158282 2 15.523812428843833 20.978094824324135 39.865713218703036 23.632379528128997 3 19.917708399027703 24.765386085036027 29.322998655815912 25.993906860120354 4 13.335092000263801 17.127258787838816 36.83679021301853 32.70085899887885 5 14.125470982995767 37.341094119106714 33.58600816226258 14.947426735634942 6 31.84350518574454 36.2673191337115 17.43017731528387 14.458998365260086 7 28.651575399522606 32.60329510573216 20.55931309852136 18.18581639622387 8 26.298759647233975 34.603665825255845 19.709334054385586 19.38824047312459 9 26.321759675262683 17.30381140966265 20.447766853909428 35.92666206116524 10-11 24.885700483330737 26.956529086815696 27.043500590664287 21.11426983918928 12-13 26.07941614179484 24.199852559810246 27.749443183488015 21.971288114906894 14-15 22.910856118513646 25.496010339297886 26.407149630257987 25.18598391193048 16-17 22.041240121941367 28.727488413426737 26.8295739141761 22.401697550455797 18-19 21.92887797784413 27.31480031194303 29.458872817264364 21.297448892948474 20-21 22.77015073272394 26.274002314906415 29.550876828512262 21.404970123857385 22-23 22.43506407857271 26.18816990673035 29.49376443684555 21.883001577851385 24-25 22.32414855876334 26.282141781734836 29.679126805783785 21.71458285371804 26-27 22.141386045482097 26.412002482243118 29.68790800943846 21.758703462836323 28-29 21.953258401956308 26.695946718823905 29.63065708023195 21.72013779898784 30-31 22.20034987961165 26.356731678368828 29.524529397081206 21.91838904493832 32-33 22.15257337105157 26.522005441747293 29.456909333202532 21.868511853998605 34-35 22.498592341181872 26.50170196874379 29.366215528846908 21.633490161227428 36-37 22.464217207686012 26.316188659358453 29.428338887419848 21.79125524553569 38-39 22.176543935037767 26.70528951680218 29.381993178141197 21.736173370018857 40-41 22.460017040599414 26.595845539785923 29.165971068903957 21.778166350710706 42-43 22.22869370965239 26.650224142234226 29.00036335134839 22.120718796764997 44-45 22.42963861842915 26.743889125783753 28.800821247745063 22.02565100804204 46-47 22.731113565917035 27.014113302116993 28.30147810459672 21.953295027369254 48-49 22.698489803800417 27.139695363973626 28.132595213957686 22.02921961826827 50-51 22.512837592196128 27.406188094529526 28.068450396071356 22.01252391720299 52-53 22.78644011207507 27.495468461626448 27.669372365189954 22.048719061108528 54-55 22.603588125648702 27.31959825259831 27.729799892992666 22.347013728760324 56-57 22.64134922484862 27.601666807841223 27.65838394492832 22.098600022381834 58-59 22.861848918643695 27.245393284270055 27.669415785038275 22.22334201204798 60-61 22.680522643157424 27.31560275064065 28.045162245012783 21.958712361189143 62-63 22.44465204928025 27.347986992203687 28.18759123500902 22.019769723507043 64-65 22.536403420006867 27.35683986946828 27.911294653879494 22.195462056645358 66-67 22.587484143676072 27.444140914416554 27.622494288631984 22.345880653275394 68-69 22.468884012260087 27.346270780401667 27.892232915265918 22.292612292072327 70-71 22.64981423733229 27.324557995729325 27.729532358688164 22.296095408250224 72-73 22.640889598216717 27.268429287691603 27.73275781052923 22.357923303562448 74-75 22.479146340608878 27.774514616831397 27.42036204186766 22.325977000692067 76-77 22.645912873225647 27.446922740036584 27.516974521472548 22.39018986526522 78-79 22.612987963696124 27.539377356942918 27.430394830831833 22.417239848529128 80-81 22.68285695441767 27.600971169938248 27.364361855610607 22.351810020033472 82-83 22.668354191411225 27.609413453248987 27.478871419394206 22.243360935945574 84-85 22.61556598426625 27.677653911075662 27.283733066226436 22.423047038431655 86-87 22.496849795530398 27.5765828412062 27.471328708224497 22.455238655038904 88-89 22.621828441647036 27.605680827516277 27.175311359387976 22.59717937144871 90-91 22.623335843556617 27.482493356564238 27.255954979207193 22.638215820671956 92-93 22.56385186992701 27.491030813442567 27.34359156566714 22.60152575096328 94-95 22.55097628725198 27.365947275418485 27.32701614415689 22.75606029317265 96-97 22.398696148419464 27.469958281860627 27.441084102796196 22.69026146692371 98-99 22.250734898134453 27.70537628247788 27.103731569838867 22.940157249548797 100-101 22.047799253358217 28.01050508581187 27.02642099566252 22.91527466516739 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 460.0 1 332.0 2 235.0 3 294.5 4 292.0 5 289.0 6 469.0 7 1015.5 8 1762.5 9 2152.5 10 2110.5 11 2003.5 12 2156.0 13 2444.5 14 2692.0 15 2966.5 16 3239.0 17 3534.5 18 3871.5 19 4186.5 20 4524.0 21 5097.5 22 6018.5 23 7181.5 24 8521.0 25 10553.0 26 13654.5 27 17491.5 28 20952.5 29 24521.5 30 28751.5 31 33407.0 32 37914.0 33 42418.5 34 47723.5 35 52715.0 36 58864.0 37 64213.5 38 67461.0 39 70209.5 40 72401.5 41 75190.0 42 77733.5 43 80247.0 44 83875.5 45 86699.5 46 88057.0 47 91132.0 48 97587.5 49 97067.0 50 90378.0 51 87349.0 52 85260.5 53 83582.0 54 81407.0 55 75975.5 56 68318.0 57 60920.0 58 56259.5 59 51248.5 60 42849.5 61 34150.0 62 26728.0 63 20562.0 64 15005.5 65 10642.0 66 7701.0 67 5912.0 68 4366.0 69 2900.0 70 2055.0 71 1344.5 72 899.5 73 660.5 74 445.5 75 277.0 76 184.0 77 99.0 78 49.5 79 32.5 80 23.0 81 12.0 82 6.5 83 5.0 84 4.5 85 4.5 86 2.0 87 2.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015869194966044047 2 0.003379932434326264 3 0.003173838993208809 4 1.648747528939641E-4 5 0.001689966217163132 6 4.121868822349102E-4 7 4.1218688223491025E-5 8 0.0 9 0.0 10-11 8.243737644698205E-5 12-13 6.388896674641109E-4 14-15 2.0609344111745512E-5 16-17 0.0 18-19 0.0 20-21 0.00280287079919739 22-23 0.0 24-25 0.003895166037119902 26-27 0.002163981131733279 28-29 0.0035654165313319737 30-31 0.016178335127720225 32-33 0.0010304672055872757 34-35 0.0028853081756443715 36-37 0.0038127286606729196 38-39 0.0024319026051859705 40-41 0.004657711769254486 42-43 0.002370074572850734 44-45 8.03764420358075E-4 46-47 1.648747528939641E-4 48-49 3.5035884989967375E-4 50-51 2.473121293409461E-4 52-53 0.0012983886790399674 54-55 0.003379932434326264 56-57 3.915775381231648E-4 58-59 0.0013808260554869494 60-61 0.0012777793349282218 62-63 2.0609344111745512E-5 64-65 2.8853081756443716E-4 66-67 6.182803233523653E-5 68-69 0.0 70-71 1.8548409700570962E-4 72-73 0.0 74-75 4.5340557045840126E-4 76-77 8.862017968050569E-4 78-79 2.8853081756443716E-4 80-81 0.005935491104182708 82-83 0.0018754503141688414 84-85 0.0036684632518907013 86-87 0.002102153099398042 88-89 0.0011953419584812398 90-91 2.060934411174551E-4 92-93 0.0 94-95 2.060934411174551E-4 96-97 0.001689966217163132 98-99 0.002782261455085644 100-101 8.655924526933115E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2426084.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.74594226808938 #Duplication Level Percentage of deduplicated Percentage of total 1 79.03561780465019 46.43021840674757 2 12.278652436581861 14.42642014338746 3 3.670299587583408 6.468456230363016 4 1.5979935661625126 3.7550255113024495 5 0.8584168905268705 2.5214254546422175 6 0.5347560549773912 1.884884899992783 7 0.3661656088583805 1.5057520602987955 8 0.25059741644419586 1.1777265087170468 9 0.18794363241692272 0.99368332016576 >10 1.1034659013470136 11.885501780906504 >50 0.07726813653756628 3.1294648399439837 >100 0.03678684336420239 3.8138259861077586 >500 0.0011935879082465343 0.4733407590054358 >1k 7.02110534261273E-4 0.8166736692482965 >5k 7.021105342612729E-5 0.2987870737391243 >10k+ 7.021105342612729E-5 0.41881335543167697 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10154 0.41853456022132784 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7244 0.298588177490969 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4943 0.20374397588871612 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4115 0.16961490203966556 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.1218688223491025E-5 7 1.648747528939641E-4 0.0 0.0 0.0 4.1218688223491025E-5 8 1.648747528939641E-4 0.0 0.0 0.0 4.1218688223491025E-5 9 1.648747528939641E-4 0.0 0.0 0.0 4.1218688223491025E-5 10-11 1.648747528939641E-4 0.0 0.0 0.0 4.1218688223491025E-5 12-13 2.2670278522920063E-4 0.0 0.0 0.0 4.1218688223491025E-5 14-15 3.297495057879282E-4 0.0 0.0 0.0 4.1218688223491025E-5 16-17 3.503588498996737E-4 0.0 0.0 2.0609344111745512E-5 4.1218688223491025E-5 18-19 5.152336027936378E-4 0.0 0.0 6.182803233523653E-5 4.1218688223491025E-5 20-21 6.182803233523654E-4 0.0 0.0 1.0304672055872757E-4 4.1218688223491025E-5 22-23 6.594990115758564E-4 0.0 0.0 2.2670278522920066E-4 4.1218688223491025E-5 24-25 9.068111409168025E-4 0.0 0.0 3.297495057879282E-4 4.1218688223491025E-5 26-27 9.274204850285481E-4 0.0 0.0 4.740149145701468E-4 4.1218688223491025E-5 28-29 0.0012777793349282218 0.0 0.0 0.0012777793349282218 4.1218688223491025E-5 30-31 0.001483872776045677 0.0 0.0 0.0033181044019910274 4.1218688223491025E-5 32-33 0.001483872776045677 0.0 0.0 0.0055851322542830335 4.1218688223491025E-5 34-35 0.0015663101524926591 0.0 0.0 0.007419363880228385 4.1218688223491025E-5 36-37 0.001731184905386623 0.0 0.0 0.009336032882620717 4.1218688223491025E-5 38-39 0.001854840970057096 0.0 0.0 0.01323119891974062 4.1218688223491025E-5 40-41 0.001854840970057096 0.0 0.0 0.018259878883006522 4.1218688223491025E-5 42-43 0.0019784970347275694 0.0 0.0 0.022896981308149267 4.1218688223491025E-5 44-45 0.0021227624435097877 0.0 0.0 0.025514368010340946 4.1218688223491025E-5 46-47 0.0024319026051859705 0.0 0.0 0.028956128477002446 4.1218688223491025E-5 48-49 0.0025143399816329524 0.0 0.0 0.03417029253727406 4.1218688223491025E-5 50-51 0.0025143399816329524 0.0 0.0 0.03979664347978058 8.243737644698205E-5 52-53 0.002534949325744698 0.0 0.0 0.04989522209453588 8.243737644698205E-5 54-55 0.0025555586698564436 0.0 0.0 0.05692300843664111 8.243737644698205E-5 56-57 0.0026379960463034256 0.0 0.0 0.07804758615118026 8.243737644698205E-5 58-59 0.0026379960463034256 4.1218688223491025E-5 0.0 0.11450551588485808 8.243737644698205E-5 60-61 0.0026379960463034256 4.1218688223491025E-5 0.0 0.14412114337343637 8.243737644698205E-5 62-63 0.0026379960463034256 4.1218688223491025E-5 0.0 0.15953693276902203 8.243737644698205E-5 64-65 0.0026379960463034256 4.1218688223491025E-5 0.0 0.18970901254861744 1.236560646704731E-4 66-67 0.0027204334227504076 4.1218688223491025E-5 0.0 0.25631841271777894 1.236560646704731E-4 68-69 0.002741042766862153 4.1218688223491025E-5 0.0 0.35332659545176504 1.236560646704731E-4 70-71 0.002823480143309135 4.1218688223491025E-5 0.0 0.4332084132288907 1.236560646704731E-4 72-73 0.002885308175644372 4.1218688223491025E-5 0.0 0.5285266297457136 1.236560646704731E-4 74-75 0.0029265268638678627 4.1218688223491025E-5 0.0 0.64301153628646 1.236560646704731E-4 76-77 0.0029265268638678627 4.1218688223491025E-5 0.0 0.7142168201925407 1.236560646704731E-4 78-79 0.002988354896203099 4.1218688223491025E-5 0.0 0.8137805615963833 1.236560646704731E-4 80-81 0.003503588498996737 4.1218688223491025E-5 0.0 0.9184554203399387 1.236560646704731E-4 82-83 0.0035860258754437193 4.1218688223491025E-5 0.0 0.9929169806156753 1.236560646704731E-4 84-85 0.0037509006283376833 4.1218688223491025E-5 0.0 1.1331017392637683 1.236560646704731E-4 86-87 0.003936384725343392 4.1218688223491025E-5 0.0 1.3685222770522372 1.236560646704731E-4 88-89 0.003998212757678629 4.1218688223491025E-5 0.0 1.6793111862573595 1.236560646704731E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13725 0.0 31.291847 1 GGTATCA 9440 0.0 27.780636 1 ATCAACG 16485 0.0 25.759836 3 TCAACGC 16685 0.0 25.364609 4 TATCAAC 16775 0.0 25.315554 2 CAACGCA 16805 0.0 25.155222 5 AACGCAG 17425 0.0 24.232914 6 GTACATG 9880 0.0 23.658285 1 TACATGG 9915 0.0 22.94855 2 GCGTATC 130 2.9390803E-4 21.92719 1 ACATGGG 10050 0.0 21.74134 3 ACGCAGA 19605 0.0 21.465624 7 GAGTACT 9815 0.0 21.462042 12-13 CGCAGAG 19550 0.0 21.38024 8 AGAGTAC 15000 0.0 20.614195 10-11 AGTACTT 10420 0.0 20.215925 12-13 GTACTTT 10510 0.0 19.839241 14-15 GCAGAGT 20755 0.0 19.52104 9 GTGTAGC 1425 0.0 18.670172 1 CATGGGG 5045 0.0 18.076597 4 >>END_MODULE