FastQCFastQC Report
Thu 26 May 2016
SRR937058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250743
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82280.36556817015536647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56000.24880672737847012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38330.17029931893601358No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29260.13000151505525065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111950.033.406041
ATCAACG133200.027.648523
GGTATCA75550.027.612421
TCAACGC133550.027.541094
TATCAAC135700.027.3848882
CAACGCA133850.027.3042765
AACGCAG138150.026.5564356
GTACATG93550.024.9408971
TACATGG93050.024.4115622
ACATGGG92400.023.0913223
ACGCAGA158300.022.9954327
CGCAGAG158150.022.8975568
GAGTACT77900.021.2886412-13
CATGGGG46600.021.1090414
GCAGAGT166150.020.8511779
AGAGTAC124450.020.23720610-11
AGTACTT82700.020.11048112-13
GTACTTT83300.019.7650914-15
GTGCGCG1801.2294266E-418.4811469
TCGTATA1350.00937940417.600312
Produced by FastQC (version 0.11.5)