##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937058_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2250743 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74155645491289 33.0 31.0 34.0 30.0 34.0 2 31.86218017783461 33.0 31.0 34.0 30.0 34.0 3 31.88355934018233 33.0 31.0 34.0 30.0 34.0 4 35.45728943731026 37.0 35.0 37.0 33.0 37.0 5 35.455158141111625 37.0 35.0 37.0 33.0 37.0 6 35.51728606953348 37.0 35.0 37.0 33.0 37.0 7 35.4530641659221 37.0 35.0 37.0 33.0 37.0 8 35.39661880543447 37.0 35.0 37.0 33.0 37.0 9 37.03716328341352 39.0 37.0 39.0 33.0 39.0 10-11 36.851009200073044 39.0 37.0 39.0 33.0 39.0 12-13 36.780422287218045 39.0 37.0 39.0 33.0 39.0 14-15 37.94904749231698 40.0 38.0 41.0 33.0 41.0 16-17 37.88433219607925 40.0 38.0 41.0 33.0 41.0 18-19 37.84301806114692 40.0 38.0 41.0 33.0 41.0 20-21 37.75819807059269 40.0 37.5 41.0 32.0 41.0 22-23 37.643798958832704 40.0 37.0 41.0 32.0 41.0 24-25 37.48210635332421 40.0 37.0 41.0 32.0 41.0 26-27 37.586897304578976 40.0 37.0 41.0 32.0 41.0 28-29 37.54483541657133 40.0 37.0 41.0 32.0 41.0 30-31 37.40289073430418 40.0 37.0 41.0 32.0 41.0 32-33 37.2133244444168 40.0 36.5 41.0 31.0 41.0 34-35 37.04618363802531 40.0 36.0 41.0 31.0 41.0 36-37 36.87269692719249 39.0 36.0 41.0 30.0 41.0 38-39 36.707448162673394 39.0 36.0 41.0 30.0 41.0 40-41 36.546124546427556 39.0 35.5 41.0 30.0 41.0 42-43 36.33292694901195 39.0 35.0 41.0 30.0 41.0 44-45 36.078431433531065 39.0 35.0 41.0 29.5 41.0 46-47 35.738537007557056 39.0 35.0 40.0 27.5 41.0 48-49 35.42870110003675 39.0 35.0 40.0 27.0 41.0 50-51 34.792019124351384 38.0 34.0 39.5 26.0 40.5 52-53 35.0274547116219 38.5 34.5 40.0 26.5 40.5 54-55 35.406647049441005 39.0 35.0 40.5 27.0 41.0 56-57 35.28664867557069 39.0 35.0 41.0 26.5 41.0 58-59 35.045706906563744 38.5 35.0 40.0 26.0 41.0 60-61 34.75801235414261 38.0 34.0 40.0 26.0 41.0 62-63 34.43298257508742 38.0 34.0 40.0 25.5 41.0 64-65 34.11641733418698 37.0 34.0 40.0 24.5 41.0 66-67 33.736059603428735 37.0 33.5 39.5 23.5 41.0 68-69 33.34344547556074 36.0 33.0 39.0 22.5 41.0 70-71 32.9263763121778 36.0 33.0 39.0 22.0 40.5 72-73 32.53204164136021 35.0 32.0 38.5 21.0 40.0 74-75 32.10075917152691 35.0 32.0 37.5 20.0 39.5 76-77 31.664938866854193 35.0 31.5 37.0 18.5 39.0 78-79 31.21885328533733 35.0 31.0 36.5 17.5 39.0 80-81 30.78351126716822 35.0 31.0 36.0 12.5 37.5 82-83 30.376191995265565 34.5 30.5 35.5 8.5 37.0 84-85 30.023264984051934 34.0 30.0 35.0 7.0 37.0 86-87 29.69367826535504 34.0 30.0 35.0 3.5 36.0 88-89 29.346964313562232 34.0 29.5 35.0 2.0 36.0 90-91 28.99447604635447 34.0 29.0 35.0 2.0 36.0 92-93 28.62163316735851 34.0 29.0 35.0 2.0 35.0 94-95 28.2037651566616 34.0 29.0 35.0 2.0 35.0 96-97 27.73914258535959 34.0 28.0 35.0 2.0 35.0 98-99 27.13739085270953 34.0 27.0 35.0 2.0 35.0 100-101 24.858771525669525 31.5 21.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2891978809078317 1101 2 0.39385710578283906 1101 3 0.26593768637457416 1101 4 0.23784512821912784 1101 5 0.2818238030454836 1101 6 0.18380107164256998 1101 7 0.12630454502501465 1101 8 0.20226453540798417 1101 9 0.25670210890189793 1101 10-11 0.29367937399169364 1101 12-13 0.34665613446528454 1101 14-15 0.5941404162742359 1101 16-17 0.6732954840797234 1101 18-19 0.5654400815203857 1101 20-21 0.5012692556092802 1101 22-23 0.6920218825099695 1101 24-25 0.6081782917105372 1101 26-27 0.6262996388549809 1101 28-29 0.6375424355502517 1101 30-31 0.5678133318500329 1101 32-33 0.7337208747286112 1101 34-35 0.5833374915060645 1101 36-37 0.5215203366161134 1101 38-39 0.5289183536308073 1101 40-41 0.5477492660373784 1101 42-43 0.6487221938266075 1101 44-45 0.7115771686780974 1101 46-47 0.5757178316325806 1101 48-49 0.502950748510024 1101 50-51 0.5331439260561339 1101 52-53 0.5887153393845779 1101 54-55 0.6956180984996081 1101 56-57 0.7155874722603315 1101 58-59 0.6560691828881673 1101 60-61 0.7086976915866146 1101 62-63 0.6510620215829093 1101 64-65 0.8148892613911158 1101 66-67 0.9260737166114481 1101 68-69 0.8824694560496553 1101 70-71 0.8407435171400053 1101 72-73 0.8069568470916195 1101 74-75 0.7391708135273802 1101 76-77 0.7140895055193965 1101 78-79 0.7028015674289705 1101 80-81 0.6393201789929677 1101 82-83 0.711293488931787 1101 84-85 0.7976341379091068 1101 86-87 0.7006105761958388 1101 88-89 0.740524193714851 1101 90-91 0.8949280244140638 1101 92-93 1.00821760836088 1101 94-95 0.8640753921337598 1101 96-97 0.951066610872985 1101 98-99 0.9879675870146762 1101 100-101 1.1290379290227044 1102 1 0.3590871955091508 1102 2 0.288389726561892 1102 3 0.3369994012149924 1102 4 0.3433815432485119 1102 5 0.3460710642348346 1102 6 0.3287715864034695 1102 7 0.4158892168398154 1102 8 0.40441392074003346 1102 9 0.3226512462790794 1102 10-11 0.5612963832668072 1102 12-13 0.5629722115152944 1102 14-15 0.5816332275619089 1102 16-17 0.6686272585432178 1102 18-19 0.522799517741042 1102 20-21 0.6116460325071458 1102 22-23 0.5518693722879107 1102 24-25 0.6187985520133026 1102 26-27 0.5203120740621543 1102 28-29 0.5822801017441392 1102 30-31 0.6341386161922102 1102 32-33 0.7279676389205747 1102 34-35 0.6491516540586559 1102 36-37 0.41456732794646456 1102 38-39 0.5220356194864948 1102 40-41 0.3511605866712415 1102 42-43 0.43721173414632375 1102 44-45 0.39531204519086316 1102 46-47 0.3270925430455307 1102 48-49 0.6531417752749178 1102 50-51 0.5175697044802021 1102 52-53 0.4246916968106049 1102 54-55 0.5274791966402788 1102 56-57 0.5732320852844168 1102 58-59 0.5092606841420633 1102 60-61 0.38252449448997794 1102 62-63 0.46428224294162135 1102 64-65 0.5517559541514032 1102 66-67 0.3332272010390369 1102 68-69 0.2782980466330187 1102 70-71 0.35738779374670315 1102 72-73 0.522743297129864 1102 74-75 0.3534535518712474 1102 76-77 0.39147512700708376 1102 78-79 0.4091510339422939 1102 80-81 0.41948244486603414 1102 82-83 0.2256954613316644 1102 84-85 0.47887186686968874 1102 86-87 0.3677301046737149 1102 88-89 0.3269699417661762 1102 90-91 0.4371618509631787 1102 92-93 0.5473847797039788 1102 94-95 0.7015898734142567 1102 96-97 0.7160353340262482 1102 98-99 0.7419136179315373 1102 100-101 0.7093264987019658 1103 1 0.2409925041590455 1103 2 0.32163560927521573 1103 3 0.31174815323384664 1103 4 0.23248501053545567 1103 5 0.2626030881376522 1103 6 0.21318708296102784 1103 7 0.16218289222846494 1103 8 0.26179364557880547 1103 9 0.21898891041495716 1103 10-11 0.4308873716906447 1103 12-13 0.31785554728805465 1103 14-15 0.35481061870517294 1103 16-17 0.39949981952287317 1103 18-19 0.34947310543039123 1103 20-21 0.4486092409701925 1103 22-23 0.49075101489696493 1103 24-25 0.5295175175016595 1103 26-27 0.5614647878817607 1103 28-29 0.40595564213554525 1103 30-31 0.35954095595731417 1103 32-33 0.511290418958815 1103 34-35 0.5300365122878077 1103 36-37 0.393010312808201 1103 38-39 0.5386923071512228 1103 40-41 0.6254459947676381 1103 42-43 0.5359425252261687 1103 44-45 0.6398492529059538 1103 46-47 0.6822282569451232 1103 48-49 0.6741982860362796 1103 50-51 0.5981213515055188 1103 52-53 0.7315682934377463 1103 54-55 0.6695856901886472 1103 56-57 0.7768045152936196 1103 58-59 0.7390104493404692 1103 60-61 0.7759923789110132 1103 62-63 1.0411734034321043 1103 64-65 0.8937312401572939 1103 66-67 0.9860592512750372 1103 68-69 0.8153420881730611 1103 70-71 0.6143469621365156 1103 72-73 0.6851763695865856 1103 74-75 0.651571519480477 1103 76-77 0.7180424177775961 1103 78-79 0.7432490464981356 1103 80-81 0.7513464834408268 1103 82-83 0.7598029418212384 1103 84-85 0.6193850921031405 1103 86-87 0.8510150129770118 1103 88-89 0.8986776851450209 1103 90-91 1.0219233301610693 1103 92-93 0.9513269017034425 1103 94-95 0.9791355542187539 1103 96-97 1.1525767057354308 1103 98-99 1.1746825159430827 1103 100-101 1.3263745012840715 1104 1 0.36161297492264666 1104 2 0.2422595465837709 1104 3 0.3072248218569591 1104 4 0.3600402704906429 1104 5 0.3614067562859873 1104 6 0.2496641420802348 1104 7 0.28819299074971383 1104 8 0.34150786633178853 1104 9 0.25488838197076547 1104 10-11 0.4837380781351186 1104 12-13 0.478949714622253 1104 14-15 0.5274661382773829 1104 16-17 0.537837138816478 1104 18-19 0.4966916619134878 1104 20-21 0.5593599994468192 1104 22-23 0.41840124798812184 1104 24-25 0.5210407955209391 1104 26-27 0.38043220949622025 1104 28-29 0.33214041034093356 1104 30-31 0.48492172363388875 1104 32-33 0.5693713600646007 1104 34-35 0.4758127821562894 1104 36-37 0.5026477835762151 1104 38-39 0.6209947965043412 1104 40-41 0.43390290306494705 1104 42-43 0.5135500042629175 1104 44-45 0.48658318980327664 1104 46-47 0.5093730711807964 1104 48-49 0.6413430017133166 1104 50-51 0.5770344775100043 1104 52-53 0.5678226440366245 1104 54-55 0.7384933818741359 1104 56-57 0.5323996717826418 1104 58-59 0.5815176653616163 1104 60-61 0.6817057547786263 1104 62-63 0.7870895925708794 1104 64-65 0.9061899007590881 1104 66-67 0.810682323986498 1104 68-69 0.7846868703941752 1104 70-71 0.6424651448105223 1104 72-73 0.6953562866681864 1104 74-75 0.7229357000793115 1104 76-77 0.6786020574744356 1104 78-79 0.6120483916609949 1104 80-81 0.7153095395876399 1104 82-83 0.6658274570912006 1104 84-85 0.6083007296534326 1104 86-87 0.7509164883317467 1104 88-89 0.8271977831191002 1104 90-91 0.7971037009522348 1104 92-93 0.8350240636566824 1104 94-95 0.9491337121919265 1104 96-97 0.9741809105864867 1104 98-99 0.9165113257184672 1104 100-101 0.9653513471427893 1105 1 0.2419424359154192 1105 2 0.31979123406124543 1105 3 0.3775954892196829 1105 4 0.3717013133466196 1105 5 0.3548413918847899 1105 6 0.3116395526963487 1105 7 0.2687506129052508 1105 8 0.33291533630377756 1105 9 0.3920966983547345 1105 10-11 0.4499957619750887 1105 12-13 0.49491862143054277 1105 14-15 0.5381486691255333 1105 16-17 0.43968966279111754 1105 18-19 0.3819646452097416 1105 20-21 0.42162543831867794 1105 22-23 0.47743007162380735 1105 24-25 0.5011736408099168 1105 26-27 0.5126254006819693 1105 28-29 0.4233015809678946 1105 30-31 0.10197482978390582 1105 32-33 0.3272722006497091 1105 34-35 0.49999483324678096 1105 36-37 0.39127377334467894 1105 38-39 0.25379295908638255 1105 40-41 0.4758015561085571 1105 42-43 0.5859933197034408 1105 44-45 0.5552502745188193 1105 46-47 0.6168719400559795 1105 48-49 0.7693091561940051 1105 50-51 0.7123591080472877 1105 52-53 0.6462824292388945 1105 54-55 0.8887682219138213 1105 56-57 1.0141222109308785 1105 58-59 0.8555974722676112 1105 60-61 0.9064054107742052 1105 62-63 0.9338208199632234 1105 64-65 0.7086264632429362 1105 66-67 0.9635381561244643 1105 68-69 0.8743717792986629 1105 70-71 0.8392526407531804 1105 72-73 0.8378091497702087 1105 74-75 0.8181401510029289 1105 76-77 0.8634769871273278 1105 78-79 0.6824069121040779 1105 80-81 0.8718025065211705 1105 82-83 0.9119274282491183 1105 84-85 0.7756981598766863 1105 86-87 0.8421983061831178 1105 88-89 0.92029953832159 1105 90-91 0.9414521228299115 1105 92-93 0.9543080018305048 1105 94-95 0.8488941347512942 1105 96-97 0.7810500027768583 1105 98-99 0.8618742985099139 1105 100-101 0.9358518678163925 1106 1 0.31337832943988175 1106 2 0.34821312053674447 1106 3 0.23762188678089657 1106 4 0.2481177741356717 1106 5 0.2039999251129032 1106 6 0.18632103817853363 1106 7 0.23438618300993141 1106 8 0.2890210982096235 1106 9 0.276208855944013 1106 10-11 0.3541289124282656 1106 12-13 0.44408373249763855 1106 14-15 0.547922007288868 1106 16-17 0.6030138439953987 1106 18-19 0.5996824011958992 1106 20-21 0.569711989936259 1106 22-23 0.3694059326413779 1106 24-25 0.5235226452043236 1106 26-27 0.37109429340678446 1106 28-29 0.3874611664628418 1106 30-31 0.5689136927997467 1106 32-33 0.5008113347624672 1106 34-35 0.32560676435975466 1106 36-37 0.3311694724312204 1106 38-39 0.4157133057666087 1106 40-41 0.45139037108368285 1106 42-43 0.42255669680719876 1106 44-45 0.5123515202040991 1106 46-47 0.4071822397711884 1106 48-49 0.48057913075781755 1106 50-51 0.49844731593098146 1106 52-53 0.6027106176964168 1106 54-55 0.5530065540737041 1106 56-57 0.5913801478619618 1106 58-59 0.512395350268001 1106 60-61 0.6226885044724284 1106 62-63 0.6469055097337844 1106 64-65 0.6117046295213342 1106 66-67 0.6362964763880186 1106 68-69 0.64382442626043 1106 70-71 0.5058948825856433 1106 72-73 0.5902518049758214 1106 74-75 0.4952352536237683 1106 76-77 0.5984703783668834 1106 78-79 0.5131348464996002 1106 80-81 0.5015390425187221 1106 82-83 0.4958946672377458 1106 84-85 0.5859548661334522 1106 86-87 0.6935832786133496 1106 88-89 0.6209075105499906 1106 90-91 0.6669968512916604 1106 92-93 0.7124168749979631 1106 94-95 0.749170066663357 1106 96-97 0.6337518548227088 1106 98-99 0.7117723232654534 1106 100-101 0.7643012650258498 1107 1 0.24312999570477345 1107 2 0.25724284546611287 1107 3 0.29311306877161414 1107 4 0.22398297958608993 1107 5 0.2748517649327695 1107 6 0.21777644420829745 1107 7 0.1837521728115803 1107 8 0.3109087156814567 1107 9 0.4691485487383744 1107 10-11 0.5293031297163822 1107 12-13 0.48056268785339995 1107 14-15 0.43252185475890315 1107 16-17 0.4684232501600647 1107 18-19 0.5406979486174421 1107 20-21 0.5294482510131431 1107 22-23 0.4142218022209292 1107 24-25 0.5834812189834153 1107 26-27 0.5053458378243292 1107 28-29 0.5644138665795069 1107 30-31 0.5137042534535183 1107 32-33 0.5562575168443118 1107 34-35 0.6172473219978727 1107 36-37 0.7354783405322607 1107 38-39 0.41745176779937054 1107 40-41 0.6326590227943498 1107 42-43 0.648566531401066 1107 44-45 0.7245585956929048 1107 46-47 0.6949347677044884 1107 48-49 0.8512741003316862 1107 50-51 0.5994847939970569 1107 52-53 0.7424975266874441 1107 54-55 0.7104510891025129 1107 56-57 0.907248881906483 1107 58-59 0.8777078985912325 1107 60-61 0.8640733705615347 1107 62-63 0.7680300452435986 1107 64-65 0.8825122961529885 1107 66-67 0.7317824312494494 1107 68-69 0.7583860209895548 1107 70-71 0.5748884798219862 1107 72-73 0.5103298060704731 1107 74-75 0.5783223713805441 1107 76-77 0.6215277116328295 1107 78-79 0.6533418799008075 1107 80-81 0.5761171378407397 1107 82-83 0.6096500214283687 1107 84-85 0.5457764297515766 1107 86-87 0.407916059609601 1107 88-89 0.541091951930138 1107 90-91 0.590517862115501 1107 92-93 0.4684335694944295 1107 94-95 0.4610899506669277 1107 96-97 0.5557864594707951 1107 98-99 0.47874663649074733 1107 100-101 0.26579556262612414 1108 1 0.2643309110590977 1108 2 0.21182571880457246 1108 3 0.23439416749952002 1108 4 0.1521853716540278 1108 5 0.2302934215008321 1108 6 0.10391737812323498 1108 7 0.13799391679049222 1108 8 0.13904914442979077 1108 9 0.11223509777879315 1108 10-11 0.20460928054550465 1108 12-13 0.2931129561876915 1108 14-15 0.4062953830827638 1108 16-17 0.3971644866073589 1108 18-19 0.30047438874431975 1108 20-21 0.3180977060670358 1108 22-23 0.4946442312398176 1108 24-25 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-3.5228811039139067 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5287.0 3 1927.0 4 1996.0 5 2069.0 6 2272.0 7 2764.0 8 3290.0 9 3871.0 10 5041.0 11 6294.0 12 7701.0 13 9302.0 14 10555.0 15 12866.0 16 15720.0 17 19655.0 18 17605.0 19 12468.0 20 12108.0 21 12796.0 22 14126.0 23 15650.0 24 17664.0 25 20504.0 26 23544.0 27 28077.0 28 33372.0 29 40249.0 30 49863.0 31 61607.0 32 78080.0 33 101942.0 34 140724.0 35 201989.0 36 307653.0 37 459725.0 38 417053.0 39 73297.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.90746927287888 18.603576133743204 14.50873053830467 22.980224055073244 2 15.157851037264908 20.037105339070706 40.83062977290092 23.974413850763465 3 19.55405381983408 24.473976473432508 29.295282909440623 26.67668679729279 4 12.818595802654068 16.50326368871277 37.60649492944788 33.07164557918529 5 14.018893106523581 36.83680065585257 34.10344438190608 15.040861855717768 6 31.504089257673918 35.84337938601561 18.04688375269194 14.605647603618536 7 28.41179682067716 32.31966261263584 21.02384581048903 18.244694756197973 8 25.95782480903524 34.26397485013807 20.263271500513287 19.514928840313402 9 26.08036499066583 17.235690090487417 20.783163196122466 35.90078172272429 10-11 24.60396168367221 26.54712210711792 27.66658241147258 21.182333797737293 12-13 25.79687804630799 23.95392631794469 28.09162538600925 22.157570249738065 14-15 22.764191858229243 25.213236323865797 26.660753623961604 25.361818193943353 16-17 21.905890197821737 28.42742831740387 27.152967326072456 22.513714158701934 18-19 21.781204257159608 27.151394271779537 29.55683702802501 21.510564443035843 20-21 22.70845872967468 26.20144883340283 29.483084052074787 21.607008384847706 22-23 22.311061611317538 26.11232872998586 29.465964435257554 22.11064522343905 24-25 22.149847989670896 26.200435000836542 29.59307713654889 22.056639872943673 26-27 21.959571299897664 26.343420438129407 29.598263621501836 22.09874464047109 28-29 21.80946887656297 26.591988311213928 29.565843184341006 22.032699627882092 30-31 22.023812168787877 26.23908771861222 29.488576930972794 22.248523181627107 32-33 21.836195596631935 26.375063873275423 29.494790162404744 22.2939503676879 34-35 22.292847592721536 26.399770898308798 29.310331037724907 21.997050471244762 36-37 22.26491417140925 26.21658170117161 29.36004953302857 22.158454594390566 38-39 21.981311276551786 26.54194187655934 29.366511815974285 22.110235030914595 40-41 22.252195009242147 26.395208303711076 29.23059149722736 22.122005189819422 42-43 22.000952625117577 26.4391015287767 29.05708774859228 22.502858097513446 44-45 22.19382376979348 26.56591177137698 28.921146865021896 22.31911759380764 46-47 22.415719341647712 26.732504561400233 28.550222277316696 22.30155381963536 48-49 22.3720605272307 26.768915314971082 28.435846711444878 22.423177446353336 50-51 22.180947066000655 27.012828499245096 28.433463046084313 22.372761388669936 52-53 22.437954553583587 27.135353524582133 27.99529109313243 22.43140082870185 54-55 22.302187043033044 26.909502602226187 28.123437897815357 22.664872456925416 56-57 22.30633945603399 27.184324286490398 28.013727470383614 22.495608787092 58-59 22.500462108030195 26.901907795229267 28.07121884547641 22.52641125126413 60-61 22.410901937972696 26.870343291862852 28.44501137729354 22.27374339287091 62-63 22.229381314458212 26.930616472641688 28.502619820430713 22.337382392469387 64-65 22.145889144583222 27.008282985642857 28.304696209804238 22.541131659969686 66-67 22.2152900396312 27.06843997229793 27.992819858570144 22.723450129500726 68-69 22.133783260993372 26.996881748887127 28.272735979258172 22.596599010861325 70-71 22.254953347777196 26.862006162230596 28.156265671371845 22.726774818620363 72-73 22.22839654846029 26.841640307897674 28.185670902140902 22.744292241501128 74-75 22.084273848346356 27.30460154862523 27.93017966123462 22.680944941793797 76-77 22.26358704407561 27.019277453789552 27.953407912966476 22.76372758916836 78-79 22.193430876732766 27.096772329669992 27.93505235444438 22.77474443915286 80-81 22.293332195573758 27.156098835751962 27.836087955926036 22.71448101274824 82-83 22.27366079107003 27.09328407793458 28.069244071552475 22.56381105944292 84-85 22.261620164972157 27.144214764659257 27.789718381243755 22.804446689124827 86-87 22.17079687803794 27.070544121323227 28.056039774463237 22.702619226175596 88-89 22.24384416999532 26.992241651114934 27.799117045370465 22.96479713351928 90-91 22.19206595697359 26.90264987287338 27.96510089363025 22.94018327652278 92-93 22.285028834079213 26.875126248400854 27.96977904946537 22.87006586805456 94-95 22.142369883498986 26.781767802346018 28.05967927224085 23.016183041914147 96-97 22.02642976005917 26.798911768052452 28.15715715626733 23.017501315621043 98-99 21.866486421728258 26.974861209989726 27.92772628433965 23.230926083942368 100-101 21.89083192790327 26.91625859735301 27.937837867276276 23.25507160746744 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 254.0 1 201.0 2 203.5 3 269.5 4 231.0 5 235.0 6 437.0 7 936.0 8 1603.0 9 1928.5 10 1820.0 11 1735.5 12 1898.5 13 2080.5 14 2224.5 15 2424.0 16 2633.0 17 2883.0 18 3088.5 19 3263.0 20 3614.0 21 4212.5 22 5116.0 23 6341.5 24 7687.0 25 9623.0 26 12666.0 27 16178.0 28 19041.5 29 22294.0 30 26245.5 31 30900.0 32 35587.5 33 39772.5 34 44628.5 35 49845.0 36 55667.0 37 60410.5 38 62975.0 39 65220.5 40 67762.5 41 70543.0 42 72861.0 43 75459.0 44 78976.5 45 81737.0 46 83063.5 47 85247.0 48 90625.0 49 90907.0 50 84537.5 51 80638.0 52 79139.0 53 77892.0 54 75602.0 55 69911.5 56 63158.5 57 56939.0 58 51427.5 59 45859.0 60 38677.5 61 31000.5 62 24123.0 63 18551.0 64 13702.0 65 9810.0 66 6956.0 67 5170.5 68 3874.0 69 2655.5 70 1844.0 71 1270.5 72 848.5 73 578.0 74 402.5 75 254.0 76 154.0 77 85.5 78 55.5 79 35.0 80 28.0 81 26.5 82 17.0 83 13.0 84 14.0 85 13.0 86 10.0 87 8.0 88 8.5 89 7.0 90 4.5 91 3.0 92 2.5 93 2.0 94 0.5 95 1.0 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0417195566086399 2 0.04140854819941682 3 0.026924442284170163 4 0.04389661547320152 5 0.0647341788911484 6 0.06375672389073297 7 0.06482303843664071 8 0.06691123775571 9 0.06575606366430996 10-11 0.07213173605338326 12-13 0.07137642991669862 14-15 0.06566720411881766 16-17 0.055226207523471134 18-19 0.04771757592937088 20-21 0.05371559525010186 22-23 0.030101171035520274 24-25 0.01788298353032754 26-27 0.013862089096800478 28-29 0.0169277434162852 30-31 0.013195642505608148 32-33 0.008574946140007988 34-35 0.009507971367677252 36-37 0.00548707693415019 38-39 0.008352797276277212 40-41 0.008130648412546435 42-43 0.005331572729538646 44-45 0.0014883973869962053 46-47 0.002821290569380867 48-49 9.108103412961854E-4 50-51 0.005731440684254044 52-53 0.005087208979434791 54-55 0.007553061366846415 56-57 0.02268139898691232 58-59 0.008308367503531056 60-61 0.018993727848981425 62-63 0.013440006255712003 64-65 0.010041128640631116 66-67 0.016416801029704414 68-69 0.026813367852304773 70-71 0.01872714921250449 72-73 0.01710546250726982 74-75 0.018860438530742957 76-77 0.025569334215412423 78-79 0.03725436444765129 80-81 0.031411849331531856 82-83 0.03118970046780108 84-85 0.021526224895512282 86-87 0.024680738760489315 88-89 0.020326621031366086 90-91 0.018993727848981425 92-93 0.03174507262712802 94-95 0.02434751546489315 96-97 0.03798745569796285 98-99 0.05382666968196724 100-101 0.06360121968612144 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2250743.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.80774806066881 #Duplication Level Percentage of deduplicated Percentage of total 1 80.86066903763167 49.97815859895061 2 11.439869900864856 14.141451933589666 3 3.2761906750416667 6.074819035250634 4 1.4157345686924525 3.5001346217009095 5 0.7448319286561449 2.301819209696052 6 0.47002501626993265 1.7430712672694253 7 0.31239975342467224 1.351610767772104 8 0.22766611628967362 1.1257223966066459 9 0.17280355224631366 0.9612538579105897 >10 0.978523240829925 11.045602784135243 >50 0.06966697846179862 2.9599769619451552 >100 0.0301803369290138 3.2452557039888608 >500 7.913920639707734E-4 0.3301705888230286 >1k 5.036131316176344E-4 0.6260567973115814 >5k 1.4388946617646697E-4 0.6148954750496872 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8228 0.36556817015536647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5600 0.24880672737847012 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3833 0.17029931893601358 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2926 0.13000151505525065 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.442977274615538E-5 0.0 0.0 0.0 0.0 3 4.442977274615538E-5 0.0 0.0 0.0 0.0 4 4.442977274615538E-5 0.0 0.0 0.0 0.0 5 1.3328931823846616E-4 0.0 0.0 0.0 0.0 6 1.777190909846215E-4 0.0 0.0 0.0 0.0 7 2.665786364769323E-4 0.0 0.0 0.0 0.0 8 2.665786364769323E-4 0.0 0.0 0.0 0.0 9 2.665786364769323E-4 0.0 0.0 0.0 0.0 10-11 3.1100840922308764E-4 0.0 0.0 0.0 0.0 12-13 3.776530683423207E-4 0.0 0.0 0.0 4.442977274615538E-5 14-15 4.887275002077092E-4 0.0 0.0 2.221488637307769E-5 4.442977274615538E-5 16-17 4.887275002077092E-4 0.0 0.0 4.442977274615538E-5 8.885954549231076E-5 18-19 4.887275002077092E-4 0.0 0.0 4.442977274615538E-5 8.885954549231076E-5 20-21 4.887275002077092E-4 0.0 0.0 8.885954549231077E-5 8.885954549231076E-5 22-23 5.331572729538646E-4 0.0 0.0 1.9993397735769923E-4 8.885954549231076E-5 24-25 7.997359094307968E-4 0.0 0.0 4.2208284108847616E-4 8.885954549231076E-5 26-27 7.997359094307968E-4 0.0 0.0 7.330912503115637E-4 8.885954549231076E-5 28-29 0.001288463409638506 0.0 0.0 0.0017549760234731376 8.885954549231076E-5 30-31 0.001421752727876972 0.0 0.0 0.00317672875135011 8.885954549231076E-5 32-33 0.0014439676142500497 0.0 0.0 0.004798415456584781 8.885954549231076E-5 34-35 0.001577256932488516 0.0 0.0 0.006464531934565608 8.885954549231076E-5 36-37 0.001643901591607749 0.0 0.0 0.008863739662857998 8.885954549231076E-5 38-39 0.0017771909098462152 0.0 0.0 0.013284502051100459 8.885954549231076E-5 40-41 0.0017771909098462152 0.0 0.0 0.01834949614416217 1.3328931823846616E-4 42-43 0.0019771248872039147 0.0 0.0 0.023347845578104653 1.3328931823846616E-4 44-45 0.002088199319069303 0.0 0.0 0.025524904442666266 1.5550420461154385E-4 46-47 0.0023325630691731574 0.0 0.0 0.029501369103447174 1.777190909846215E-4 48-49 0.0023992077282923904 0.0 0.0 0.03401099103718194 1.777190909846215E-4 50-51 0.0023992077282923904 0.0 0.0 0.03952028285770521 1.777190909846215E-4 52-53 0.0023992077282923904 0.0 0.0 0.050649940930617136 1.777190909846215E-4 54-55 0.0023992077282923904 0.0 0.0 0.05702561331969043 1.777190909846215E-4 56-57 0.002488067273784701 0.0 0.0 0.0769745812827142 1.999339773576992E-4 58-59 0.002488067273784701 0.0 0.0 0.11378464800290393 2.221488637307769E-4 60-61 0.002488067273784701 0.0 0.0 0.14484105915246653 2.221488637307769E-4 62-63 0.002488067273784701 0.0 0.0 0.16108014109118635 2.221488637307769E-4 64-65 0.0025324970465308567 0.0 0.0 0.19080365905836427 2.221488637307769E-4 66-67 0.0027102161375154783 0.0 0.0 0.25973645147402435 2.221488637307769E-4 68-69 0.0027102161375154783 0.0 0.0 0.3609474737897663 2.221488637307769E-4 70-71 0.0027102161375154783 0.0 0.0 0.44640814166699616 2.221488637307769E-4 72-73 0.0027546459102616334 0.0 0.0 0.5486410487558997 2.221488637307769E-4 74-75 0.002799075683007789 0.0 0.0 0.6694233859663231 2.221488637307769E-4 76-77 0.002843505455753944 0.0 0.0 0.7449317847484143 2.221488637307769E-4 78-79 0.002843505455753944 0.0 0.0 0.8466981792234831 2.221488637307769E-4 80-81 0.0030656543194847214 0.0 0.0 0.9666363507517295 2.221488637307769E-4 82-83 0.0030656543194847214 0.0 0.0 1.0491868685140862 2.221488637307769E-4 84-85 0.003154513864977032 0.0 0.0 1.1959161930082645 2.221488637307769E-4 86-87 0.003221158524096265 0.0 0.0 1.4384361075431535 2.221488637307769E-4 88-89 0.003243373410469343 0.0 0.0 1.765728028477707 2.221488637307769E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11195 0.0 33.40604 1 ATCAACG 13320 0.0 27.64852 3 GGTATCA 7555 0.0 27.61242 1 TCAACGC 13355 0.0 27.54109 4 TATCAAC 13570 0.0 27.384888 2 CAACGCA 13385 0.0 27.304276 5 AACGCAG 13815 0.0 26.556435 6 GTACATG 9355 0.0 24.940897 1 TACATGG 9305 0.0 24.411562 2 ACATGGG 9240 0.0 23.091322 3 ACGCAGA 15830 0.0 22.995432 7 CGCAGAG 15815 0.0 22.897556 8 GAGTACT 7790 0.0 21.28864 12-13 CATGGGG 4660 0.0 21.109041 4 GCAGAGT 16615 0.0 20.851177 9 AGAGTAC 12445 0.0 20.237206 10-11 AGTACTT 8270 0.0 20.110481 12-13 GTACTTT 8330 0.0 19.76509 14-15 GTGCGCG 180 1.2294266E-4 18.481146 9 TCGTATA 135 0.009379404 17.60031 2 >>END_MODULE