FastQCFastQC Report
Thu 26 May 2016
SRR937058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250743
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94940.4218162624519992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65960.29305878103364086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45510.20219989576775316No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39290.1745645771196445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129900.030.619321
GGTATCA89800.026.2472971
ATCAACG156250.025.2642143
TATCAAC158650.025.0334072
TCAACGC158000.024.9504414
CAACGCA159100.024.7779395
AACGCAG164350.023.9842996
GTACATG87500.022.2123491
GAGTACT91750.021.22249412-13
ACGCAGA185150.021.161627
CGCAGAG185150.021.0333678
TACATGG89050.021.0191942
AGAGTAC140500.020.6187610-11
AGTACTT97400.020.6009112-13
ACATGGG89700.019.8063283
GCAGAGT194100.019.7205289
GTACTTT97700.019.6147214-15
TATACCG1951.0341135E-519.485555
TACTTTT110700.017.82610714-15
ACTTTTT114700.016.89408716-17
Produced by FastQC (version 0.11.5)