FastQCFastQC Report
Thu 26 May 2016
SRR937057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339941
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86560.36992385705451547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55540.23735641197790885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37440.16000403428975346No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28770.12295181801592434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA120500.031.5828041
GGTATCA81400.029.0676651
ATCAACG143550.026.0486983
TATCAAC145250.025.841962
CAACGCA145900.025.6296835
TCAACGC145900.025.5645484
GTACATG94250.025.3566471
AACGCAG151300.024.8096836
TACATGG94100.024.7917042
GTATACG1351.3408289E-524.6359621
ACATGGG96900.022.7514293
ACGCAGA169400.021.87747
CGCAGAG168100.021.8769998
GAGTACT79750.021.83219512-13
AGAGTAC127800.020.8919110-11
GTATAGG5700.020.8386881
CATGGGG47150.020.5575924
GCAGAGT174350.020.3284159
GTACTTT85250.020.11523614-15
AGTACTT86650.019.84696612-13
Produced by FastQC (version 0.11.5)