##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937057_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2339941 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.709414895503777 33.0 31.0 34.0 30.0 34.0 2 31.83364580559937 33.0 31.0 34.0 30.0 34.0 3 31.84803334784937 33.0 31.0 34.0 30.0 34.0 4 35.40877825552012 37.0 35.0 37.0 33.0 37.0 5 35.3942445557388 37.0 35.0 37.0 33.0 37.0 6 35.48852471066578 37.0 35.0 37.0 33.0 37.0 7 35.42944458856014 37.0 35.0 37.0 33.0 37.0 8 35.38955084764958 37.0 35.0 37.0 33.0 37.0 9 36.98519663529978 39.0 37.0 39.0 33.0 39.0 10-11 36.85836352284096 39.0 37.0 39.0 33.0 39.0 12-13 36.75119522244364 39.0 37.0 39.0 32.5 39.0 14-15 37.92240082121729 40.0 38.0 41.0 33.0 41.0 16-17 37.838594220965405 40.0 38.0 41.0 33.0 41.0 18-19 37.77205386802488 40.0 38.0 41.0 32.0 41.0 20-21 37.65216366566507 40.0 37.0 41.0 32.0 41.0 22-23 37.52027957115158 40.0 37.0 41.0 32.0 41.0 24-25 37.6510264575047 40.0 37.0 41.0 32.0 41.0 26-27 37.59910356714123 40.0 37.0 41.0 32.0 41.0 28-29 37.50806409221429 40.0 37.0 41.0 32.0 41.0 30-31 37.33232782365025 40.0 37.0 41.0 31.5 41.0 32-33 37.13354524750838 40.0 36.5 41.0 31.0 41.0 34-35 36.94258786866848 39.0 36.0 41.0 30.5 41.0 36-37 36.75226832642362 39.0 36.0 41.0 30.0 41.0 38-39 36.591161486550305 39.0 36.0 41.0 30.0 41.0 40-41 36.406555549904894 39.0 35.0 41.0 30.0 41.0 42-43 36.183667024083086 39.0 35.0 40.5 30.0 41.0 44-45 35.91913257641966 39.0 35.0 40.0 28.5 41.0 46-47 35.58169864111959 39.0 35.0 40.0 27.0 41.0 48-49 35.33952565470668 38.5 35.0 40.0 26.0 41.0 50-51 34.6421050787178 38.0 34.0 39.5 25.0 40.5 52-53 34.837875826783666 38.0 34.5 39.5 26.0 40.5 54-55 35.2393207777461 39.0 35.0 40.0 26.0 41.0 56-57 35.12445677049122 39.0 35.0 40.0 26.0 41.0 58-59 34.87094439560656 38.0 34.0 40.0 26.0 41.0 60-61 34.57820261280092 38.0 34.0 40.0 25.5 41.0 62-63 34.229892548572806 37.5 34.0 40.0 24.0 41.0 64-65 33.88829312363004 37.0 33.5 40.0 23.0 41.0 66-67 33.499543791916125 36.5 33.0 39.5 22.5 41.0 68-69 33.09373334626814 36.0 33.0 39.0 22.0 41.0 70-71 32.69704984014554 35.5 32.0 39.0 20.5 40.0 72-73 32.28549138632128 35.0 32.0 38.0 20.0 40.0 74-75 31.830282686614748 35.0 31.5 37.0 18.5 39.5 76-77 31.387387972602728 35.0 31.0 37.0 17.0 39.0 78-79 30.949032475605154 35.0 31.0 36.0 12.5 39.0 80-81 30.501354093970747 34.5 30.5 36.0 7.5 37.5 82-83 30.07540104643664 34.0 30.0 35.0 7.0 37.0 84-85 29.67080879389694 34.0 30.0 35.0 3.5 37.0 86-87 29.31451818656966 34.0 29.0 35.0 2.0 36.0 88-89 28.953304805548516 34.0 29.0 35.0 2.0 36.0 90-91 28.56483325861635 34.0 29.0 35.0 2.0 35.5 92-93 28.177691232385776 34.0 29.0 35.0 2.0 35.0 94-95 27.742726205489795 34.0 27.0 35.0 2.0 35.0 96-97 27.215878306333366 34.0 26.5 35.0 2.0 35.0 98-99 26.53249034911564 33.0 25.0 35.0 2.0 35.0 100-101 23.90949707706305 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4101844778690342 1101 2 0.4449151397505524 1101 3 0.38180144448498154 1101 4 0.32302858177467897 1101 5 0.40504896340853946 1101 6 0.2627811052049296 1101 7 0.32304368617059964 1101 8 0.3066613126107498 1101 9 0.36741031657376766 1101 10-11 0.4818855803161881 1101 12-13 0.4962062628823247 1101 14-15 0.5360062923420017 1101 16-17 0.6036510615884083 1101 18-19 0.6319542382172614 1101 20-21 0.61346367568148 1101 22-23 0.5670534834062764 1101 24-25 0.5616039170908138 1101 26-27 0.5757004498824827 1101 28-29 0.5349696085067066 1101 30-31 0.503338410205501 1101 32-33 0.6410422630652945 1101 34-35 0.5285837171427161 1101 36-37 0.5383561128973966 1101 38-39 0.4811395815677386 1101 40-41 0.5149624677600428 1101 42-43 0.5706433813434231 1101 44-45 0.6204420953090235 1101 46-47 0.48842271026174444 1101 48-49 0.6636889710813918 1101 50-51 0.7014208932837533 1101 52-53 0.7275628100445957 1101 54-55 0.6729544619488834 1101 56-57 0.6219204347611154 1101 58-59 0.5894974148233203 1101 60-61 0.7785737249036515 1101 62-63 0.7059278521304151 1101 64-65 0.7001867052569111 1101 66-67 0.6990761103393481 1101 68-69 0.6797738124578458 1101 70-71 0.5648614003808632 1101 72-73 0.5502955483139829 1101 74-75 0.6265455920998448 1101 76-77 0.6894322177084824 1101 78-79 0.5771076323216384 1101 80-81 0.5050440859528713 1101 82-83 0.4969071428882188 1101 84-85 0.5155180305501261 1101 86-87 0.5971424074571345 1101 88-89 0.608121771915684 1101 90-91 0.6298829547435041 1101 92-93 0.6241373079287769 1101 94-95 0.7995257305870531 1101 96-97 0.6979407107653763 1101 98-99 0.667399634235931 1101 100-101 0.8056435991020443 1102 1 0.22850369754610256 1102 2 0.32437729004722016 1102 3 0.1771026072543158 1102 4 0.18255061561071528 1102 5 0.2292066444827725 1102 6 0.1878892078519243 1102 7 0.20290701944270495 1102 8 0.24217959913328002 1102 9 0.32150058274324067 1102 10-11 0.4094195173833626 1102 12-13 0.1802882629190634 1102 14-15 0.2626250766694085 1102 16-17 0.4118393089845753 1102 18-19 0.3194069834917883 1102 20-21 0.3956529150135779 1102 22-23 0.36565007775958946 1102 24-25 -0.005505691829263526 1102 26-27 0.1867546573440464 1102 28-29 0.20089771152108682 1102 30-31 0.17429893482939463 1102 32-33 0.4544190701337598 1102 34-35 0.3465326140732259 1102 36-37 0.22590143885856406 1102 38-39 0.19538855748573525 1102 40-41 0.29815356237971 1102 42-43 0.5205410649893452 1102 44-45 0.28559061016050435 1102 46-47 0.3684462228170844 1102 48-49 0.25777960463454974 1102 50-51 0.21274696466639398 1102 52-53 0.4310738958470566 1102 54-55 0.5795151996553045 1102 56-57 0.501649305607387 1102 58-59 0.5179285028300811 1102 60-61 0.6479162918134023 1102 62-63 0.5608788982554387 1102 64-65 0.5221996004239813 1102 66-67 0.4448477079072717 1102 68-69 0.49379060189188806 1102 70-71 0.3988565141779645 1102 72-73 0.3505931290924664 1102 74-75 0.4160182128313039 1102 76-77 0.5689755597977957 1102 78-79 0.4684564814922716 1102 80-81 0.3829397122501952 1102 82-83 0.40008427692815474 1102 84-85 0.35094777760646423 1102 86-87 0.33821884163116067 1102 88-89 0.44281271039728765 1102 90-91 0.3519101778739788 1102 92-93 0.3006343045672182 1102 94-95 0.5141090256512548 1102 96-97 0.4307179476727434 1102 98-99 0.12210857451771417 1102 100-101 0.38840522220312934 1103 1 0.23699741648321293 1103 2 0.40870476627310026 1103 3 0.2178101562700867 1103 4 0.31818870115839815 1103 5 0.3126395095084078 1103 6 0.2925141051242619 1103 7 0.26294850185181673 1103 8 0.3077664186848068 1103 9 0.3027575780079843 1103 10-11 0.3253436750165193 1103 12-13 0.43562305726806727 1103 14-15 0.6137267085717255 1103 16-17 0.6189531508035415 1103 18-19 0.5940095741856481 1103 20-21 0.5733208992033028 1103 22-23 0.5699372456068019 1103 24-25 0.4357912204706622 1103 26-27 0.5099668046457353 1103 28-29 0.6254713427972618 1103 30-31 0.6820792345985041 1103 32-33 0.7045172973477847 1103 34-35 0.5707552102458848 1103 36-37 0.724832679808749 1103 38-39 0.7518300712023276 1103 40-41 0.5284697679133075 1103 42-43 0.676935226144586 1103 44-45 0.7330903166528984 1103 46-47 0.6348532983072417 1103 48-49 0.7553200108490756 1103 50-51 0.673430250386275 1103 52-53 0.6806442007036182 1103 54-55 0.7977588501073072 1103 56-57 0.8131481511441123 1103 58-59 0.8562030694171696 1103 60-61 0.858653582933826 1103 62-63 0.9147606342551171 1103 64-65 0.8685338860264551 1103 66-67 0.8174313575765879 1103 68-69 0.9605393202419918 1103 70-71 0.8788365556603708 1103 72-73 0.6897607415058857 1103 74-75 0.7443240868969809 1103 76-77 0.6737550377455896 1103 78-79 0.5647982026209064 1103 80-81 0.7880440052881923 1103 82-83 0.7613541865278108 1103 84-85 0.782667340867139 1103 86-87 0.8250160865730471 1103 88-89 0.8409643951309782 1103 90-91 0.7529812849846884 1103 92-93 0.8096942975230377 1103 94-95 0.9935928435982682 1103 96-97 0.7999653941565192 1103 98-99 0.615459648755575 1103 100-101 0.801674896996694 1104 1 0.3247106230428507 1104 2 0.3091387988989531 1104 3 0.2942180894928903 1104 4 0.35987083289687405 1104 5 0.35273614559598343 1104 6 0.2971627222037654 1104 7 0.32430890690638137 1104 8 0.34691408711579186 1104 9 0.4380246437080757 1104 10-11 0.44681719982420276 1104 12-13 0.41473659350101144 1104 14-15 0.6045078236845214 1104 16-17 0.6878259904141188 1104 18-19 0.5242726585938229 1104 20-21 0.6057861406897942 1104 22-23 0.5202214412342485 1104 24-25 0.4900456178434851 1104 26-27 0.32430950850942253 1104 28-29 0.3106900585394001 1104 30-31 0.26058560386771035 1104 32-33 0.4057368971122557 1104 34-35 0.38294019750415487 1104 36-37 0.4488154557346107 1104 38-39 0.3942373725246924 1104 40-41 0.45567475737365726 1104 42-43 0.42603101810254174 1104 44-45 0.3840674485589233 1104 46-47 0.32004289237504224 1104 48-49 0.5034956376831445 1104 50-51 0.41929696726455745 1104 52-53 0.3628205392235415 1104 54-55 0.31160975670891133 1104 56-57 0.4180465763397052 1104 58-59 0.5387146662263902 1104 60-61 0.42486932906302854 1104 62-63 0.6013796637134874 1104 64-65 0.499604877487819 1104 66-67 0.512424743366168 1104 68-69 0.495085365199472 1104 70-71 0.4050917796100322 1104 72-73 0.3788115504501448 1104 74-75 0.4234897230021062 1104 76-77 0.31355207962176124 1104 78-79 0.40622486147924164 1104 80-81 0.4284491715530123 1104 82-83 0.38860928122511496 1104 84-85 0.4624113414032962 1104 86-87 0.5692921217488376 1104 88-89 0.4293253794276133 1104 90-91 0.27770143766204924 1104 92-93 0.18010579874933796 1104 94-95 0.2699414962620601 1104 96-97 0.3198653113392673 1104 98-99 0.4901300153376127 1104 100-101 0.8599054800205934 1105 1 0.2760554340242791 1105 2 0.28132579420381987 1105 3 0.24865793063043995 1105 4 0.3275116410028289 1105 5 0.36248063494787885 1105 6 0.25970334730492084 1105 7 0.2797841147135074 1105 8 0.2963308987667759 1105 9 0.4671205772312632 1105 10-11 0.3984516719490543 1105 12-13 0.47329619769675446 1105 14-15 0.45968521891129654 1105 16-17 0.5969854380553201 1105 18-19 0.5157212813112722 1105 20-21 0.5439703225748573 1105 22-23 0.5181310840654518 1105 24-25 0.3741137350074766 1105 26-27 0.27651332328127154 1105 28-29 0.29190627976470296 1105 30-31 0.08524777628603886 1105 32-33 0.3704701916563735 1105 34-35 0.12809357282801415 1105 36-37 0.2360856787200234 1105 38-39 0.3847903435305682 1105 40-41 0.2821086932480128 1105 42-43 0.29657655553465645 1105 44-45 0.23136855978762583 1105 46-47 0.12539110716841861 1105 48-49 0.10271413963353382 1105 50-51 0.14034246788102678 1105 52-53 0.13346535310660812 1105 54-55 0.2896141272879973 1105 56-57 0.3627323677981096 1105 58-59 0.27833655360607423 1105 60-61 0.1423898363843108 1105 62-63 0.09965301829311102 1105 64-65 0.1183244343448493 1105 66-67 0.2765782627658311 1105 68-69 0.39219838118249584 1105 70-71 0.2058991744057579 1105 72-73 0.17862810957431208 1105 74-75 0.22757320713422757 1105 76-77 0.20982596723156277 1105 78-79 0.2573756816798429 1105 80-81 0.3162313081318473 1105 82-83 0.3452011184835193 1105 84-85 0.38858313981322823 1105 86-87 0.25762478222522134 1105 88-89 0.3240172006011903 1105 90-91 0.2707910821671504 1105 92-93 0.3646334489768819 1105 94-95 0.4330455433163145 1105 96-97 0.2857962465288004 1105 98-99 0.04902758548819364 1105 100-101 0.11852331835009267 1106 1 0.18951063293968673 1106 2 0.36542251325749575 1106 3 0.3546649211033106 1106 4 0.346539727512841 1106 5 0.3661327652346813 1106 6 0.2665421262296519 1106 7 0.31587812837923224 1106 8 0.37921819034674087 1106 9 0.36468735977226174 1106 10-11 0.3431330510155348 1106 12-13 0.4488784935433401 1106 14-15 0.5704176729337789 1106 16-17 0.47234098193995777 1106 18-19 0.41721731351054814 1106 20-21 0.5864168338741749 1106 22-23 0.3966315876498996 1106 24-25 0.5184833346089803 1106 26-27 0.3373666214321247 1106 28-29 0.4263938079585259 1106 30-31 0.3000386887788977 1106 32-33 0.21227914012867188 1106 34-35 0.2742927340645096 1106 36-37 0.3416479871862421 1106 38-39 0.23403489514496556 1106 40-41 0.1422536918574906 1106 42-43 0.09271513781310858 1106 44-45 0.2727942748515346 1106 46-47 0.4171247376258407 1106 48-49 0.19660883498960402 1106 50-51 0.07990516257509483 1106 52-53 0.24256485590550625 1106 54-55 0.06630513371992919 1106 56-57 0.10219721451998254 1106 58-59 -0.031584481269369746 1106 60-61 0.14412759270180686 1106 62-63 0.2918046856982244 1106 64-65 0.40623460642781595 1106 66-67 0.2868917474428656 1106 68-69 0.04373919537014359 1106 70-71 0.12296553189889892 1106 72-73 0.30177445352615706 1106 74-75 0.4932143139692613 1106 76-77 0.4109563560647338 1106 78-79 0.28072516282738746 1106 80-81 0.18871587381110544 1106 82-83 0.28448848687871475 1106 84-85 0.3617718630361537 1106 86-87 0.464357626420302 1106 88-89 0.4582341541385553 1106 90-91 0.22220284891924535 1106 92-93 0.057843567142157326 1106 94-95 0.001673604292978581 1106 96-97 0.18693061598316518 1106 98-99 0.24635809485170768 1106 100-101 0.31011142303469086 1107 1 0.17536634711381183 1107 2 0.16405523949353906 1107 3 0.20890055769156035 1107 4 0.24928345403217378 1107 5 0.3201312598800925 1107 6 0.23576028853797482 1107 7 0.0838971184526045 1107 8 0.2729801837789054 1107 9 0.31527162957269184 1107 10-11 0.2546162572408335 1107 12-13 0.31597443704106354 1107 14-15 0.4000758400943738 1107 16-17 0.4606092410236542 1107 18-19 0.3775755893432091 1107 20-21 0.5345186364481052 1107 22-23 0.397759343732055 1107 24-25 0.41033159832176835 1107 26-27 0.21879843970393154 1107 28-29 0.21780734743881425 1107 30-31 0.3660513853037983 1107 32-33 0.4089177034833327 1107 34-35 0.32314125658885473 1107 36-37 0.4166773329617328 1107 38-39 0.3605492937428565 1107 40-41 0.3677198676340936 1107 42-43 0.5509407379841633 1107 44-45 0.47240206194788925 1107 46-47 0.6812270880595719 1107 48-49 0.5268198114075133 1107 50-51 0.3492651852957991 1107 52-53 0.643798278377048 1107 54-55 0.718642194923639 1107 56-57 0.6212744883091403 1107 58-59 0.5962436829719451 1107 60-61 0.6271490683020602 1107 62-63 0.5717012006691604 1107 64-65 0.5226441953421883 1107 66-67 0.5825997950425901 1107 68-69 0.7497096433373258 1107 70-71 0.5514097144521202 1107 72-73 0.5216985882622396 1107 74-75 0.6710256860556996 1107 76-77 0.49723293599413054 1107 78-79 0.5347985652419318 1107 80-81 0.5663375107456403 1107 82-83 0.39131574869918495 1107 84-85 0.500296161986185 1107 86-87 0.5259734060784318 1107 88-89 0.5154050522002755 1107 90-91 0.5553999672198877 1107 92-93 0.4246070871665779 1107 94-95 0.39317945563002965 1107 96-97 0.41767399190101884 1107 98-99 0.2618805792411223 1107 100-101 0.21370303638821397 1108 1 -0.06165950150221278 1108 2 0.08269102379856363 1108 3 0.036615942818162495 1108 4 0.1777915314419758 1108 5 0.3020561221714644 1108 6 0.20393203366141677 1108 7 0.16921209550725536 1108 8 0.26240190672073993 1108 9 0.22736079579834012 1108 10-11 0.11927523637204018 1108 12-13 0.253202755870241 1108 14-15 0.2902635852011102 1108 16-17 0.3092738909909585 1108 18-19 0.31775467057589424 1108 20-21 0.4056654306581322 1108 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-1.974508933208 2316 50-51 -1.9459767532110135 2316 52-53 -1.615705779311476 2316 54-55 -1.492568458148675 2316 56-57 -1.6653749277148293 2316 58-59 -1.7926688939882567 2316 60-61 -1.6922353750386776 2316 62-63 -1.3439701765396848 2316 64-65 -1.3846181768593695 2316 66-67 -1.5240334245879978 2316 68-69 -1.225178137678693 2316 70-71 -1.1981900612661 2316 72-73 -1.8661286975845819 2316 74-75 -1.9320896597537036 2316 76-77 -2.3130767634120524 2316 78-79 -2.4987374963324775 2316 80-81 -2.600252429997738 2316 82-83 -2.2763676828745645 2316 84-85 -2.1471741862753184 2316 86-87 -2.444622408786014 2316 88-89 -2.406011593381404 2316 90-91 -2.2366807851549098 2316 92-93 -2.786357574395957 2316 94-95 -2.1698880819246718 2316 96-97 -2.284568513557648 2316 98-99 -2.3263770746089953 2316 100-101 -2.473169294799135 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5417.0 3 1956.0 4 2021.0 5 2202.0 6 2475.0 7 2857.0 8 3651.0 9 4314.0 10 5448.0 11 6816.0 12 8288.0 13 10089.0 14 11366.0 15 13631.0 16 16721.0 17 21465.0 18 19240.0 19 13551.0 20 13414.0 21 14321.0 22 15637.0 23 17289.0 24 19914.0 25 22892.0 26 26444.0 27 30885.0 28 37124.0 29 44616.0 30 53989.0 31 67246.0 32 85663.0 33 111664.0 34 152011.0 35 215832.0 36 325122.0 37 467739.0 38 400890.0 39 65719.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.03080839989706 18.579894860222574 14.46774804412485 22.921548695755508 2 15.229043636660489 20.010838964024128 40.794808922655704 23.965308476659683 3 19.549206077832153 24.57611181159575 29.236509734361977 26.638172376210118 4 12.761478286033531 16.47865150576698 37.63697194070886 33.122898267490626 5 14.090592090156804 36.88348261263507 33.96606890807254 15.059856389135584 6 31.62921048074888 35.797653225478896 18.024074853941183 14.549061439831036 7 28.486315723784966 32.31028777350494 20.993527479042207 18.209869023667892 8 25.885743237609855 34.28408345109039 20.24896797792082 19.58120533337894 9 26.106194879414936 17.206791937598506 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34-35 22.338704266475094 26.423059384830644 29.201590980285403 22.036645368408863 36-37 22.2946219584169 26.236088858650707 29.28432810912754 22.18496107380485 38-39 22.051182552700112 26.547733316010934 29.282250213467194 22.118833917821753 40-41 22.251335023262346 26.455800906048683 29.119479747455117 22.17338432323386 42-43 22.04816746408723 26.423684704803392 28.9892413931038 22.538906438005583 44-45 22.23758349307749 26.499381714783652 28.87700729014575 22.38602750199311 46-47 22.49266594599307 26.72824373098376 28.4676255656928 22.31146475733037 48-49 22.379126519385657 26.800014188482884 28.357606180708277 22.46325311142318 50-51 22.209992563911896 27.026120327871656 28.36606921544997 22.39781789276648 52-53 22.4856186599183 27.106237829468427 27.94761113018818 22.460532380425093 54-55 22.325061594644204 26.934819724816712 28.056152092294738 22.683966588244346 56-57 22.377075172462717 27.234087744655174 27.929558456122116 22.45927862675999 58-59 22.57316489452631 26.930920715238127 27.939865465485997 22.556048924749565 60-61 22.473656588588053 26.85455223038604 28.347074872727646 22.32471630829826 62-63 22.22435195151226 26.9378844864749 28.480136143122937 22.3576274188899 64-65 22.20929675987815 27.02246922106472 28.169179011860273 22.599055007196853 66-67 22.25864689419597 27.08515337968717 27.924732570723226 22.731467155393634 68-69 22.161713484358696 27.01855482566089 28.240212993036867 22.579518696943545 70-71 22.316951967324187 26.83892286088627 28.104939630537608 22.73918554125193 72-73 22.26731653773112 26.871788098605663 28.104850481355104 22.75604488230811 74-75 22.15804951443031 27.299617015456164 27.854495794009026 22.6878376761045 76-77 22.320855267489115 27.04121241553729 27.87897817970091 22.75895413727269 78-79 22.199071199688646 27.094347671555454 27.885317590163066 22.82126353859283 80-81 22.283802489458015 27.14094992758232 27.796182973560867 22.779064609398798 82-83 22.348502904158803 27.095834782402378 27.930413050406656 22.625249263032163 84-85 22.296298148550658 27.146946248789533 27.733890035505578 22.82286556715423 86-87 22.24211160250418 27.099615448235536 27.954566728514664 22.70370622074562 88-89 22.28300835583161 27.023108632649656 27.759209975451178 22.934673036067554 90-91 22.221497916028596 26.909701554263727 27.899953983996344 22.968846545711333 92-93 22.282570476536748 26.883586934937814 27.918213936770275 22.915628651755164 94-95 22.187651265743657 26.822652702935184 27.938062922275186 23.051633109045973 96-97 22.067660929373893 26.807178832642514 28.072134555879508 23.053025682104085 98-99 21.895722117157014 27.090101774557045 27.792836965777624 23.221339142508317 100-101 22.024960036664947 26.87996548980577 27.761146873967245 23.33392759956204 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 233.0 1 188.5 2 197.0 3 270.5 4 259.5 5 249.5 6 411.0 7 881.0 8 1585.5 9 1927.5 10 1819.0 11 1798.0 12 1956.5 13 2154.0 14 2281.5 15 2442.0 16 2588.5 17 2777.0 18 3044.5 19 3322.5 20 3782.0 21 4365.0 22 5280.5 23 6516.0 24 7887.0 25 9852.0 26 12966.5 27 16748.5 28 19769.5 29 23028.5 30 27157.5 31 31988.5 32 36733.5 33 41116.5 34 46231.5 35 51588.0 36 57459.5 37 62602.5 38 65810.5 39 67978.0 40 70114.5 41 72608.5 42 74842.0 43 78064.5 44 82106.0 45 84850.5 46 86255.5 47 88539.0 48 93880.0 49 93794.0 50 87806.0 51 84484.0 52 82656.5 53 81242.5 54 78987.0 55 73304.5 56 65949.0 57 59204.0 58 54237.0 59 48495.5 60 40601.5 61 32629.0 62 25496.0 63 19755.5 64 14476.0 65 10348.0 66 7449.5 67 5478.0 68 4090.0 69 2863.5 70 1981.5 71 1384.5 72 928.0 73 606.0 74 417.0 75 301.0 76 194.5 77 99.5 78 65.5 79 41.5 80 34.0 81 29.0 82 21.0 83 15.0 84 14.0 85 13.5 86 12.0 87 11.0 88 7.5 89 5.5 90 5.0 91 3.5 92 2.5 93 2.0 94 1.5 95 2.0 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024744213636155785 2 0.010000252143109591 3 0.01243621099848244 4 0.021453532375388953 5 0.053847511539820876 6 0.04213781458592332 7 0.01529953105655228 8 0.046411426612893235 9 0.02726564473206803 10-11 0.02250056732199658 12-13 0.03510772280155782 14-15 0.018590212317319114 16-17 0.017414969009902387 18-19 0.01621835764235081 20-21 0.013696926546438563 22-23 0.007542925227601892 24-25 0.0017308128709228139 26-27 4.2736120269699106E-5 28-29 0.0025855352763167957 30-31 0.0 32-33 1.7094448107879642E-4 34-35 0.0 36-37 0.0 38-39 1.2820836080909732E-4 40-41 2.1368060134849553E-5 42-43 2.1368060134849553E-5 44-45 6.410418040454866E-5 46-47 2.1368060134849553E-5 48-49 6.410418040454866E-4 50-51 4.2736120269699106E-5 52-53 7.051459844500353E-4 54-55 0.001965861532406159 56-57 6.410418040454866E-4 58-59 8.974585256636813E-4 60-61 0.0032906812607668315 62-63 0.002158174073619805 64-65 0.001239347487821274 66-67 0.010983182909312671 68-69 0.014273864170079502 70-71 0.02547072768074067 72-73 0.01709444810787964 74-75 0.017991906633543324 76-77 0.03175293736038644 78-79 0.02015008070716313 80-81 0.04609090571087049 82-83 0.03399658367454564 84-85 0.01920988606122975 86-87 0.014273864170079502 88-89 0.013568718185629467 90-91 0.022970664644963272 92-93 0.03164609705971219 94-95 0.013184093103202175 96-97 0.019316726361903996 98-99 0.027906686536113518 100-101 0.03940270288866258 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2339941.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.490215667289185 #Duplication Level Percentage of deduplicated Percentage of total 1 80.76485580754992 49.66248401943758 2 11.388133696352641 14.005175940732942 3 3.3206355044093376 6.125597799557633 4 1.4515615601670129 3.570273335560655 5 0.773725227980145 2.3788265567860805 6 0.48985982989310634 1.8072951952121223 7 0.31459252250632164 1.3541053439361173 8 0.22646007985901573 1.1140063320449929 9 0.17367906563604188 0.961160688256814 >10 0.9947364398978672 11.185604077915984 >50 0.0681986393082982 2.8810070843348132 >100 0.0321016261342152 3.4030534472829563 >500 8.342858891842226E-4 0.3454655733269621 >1k 4.866667686901159E-4 0.598462140947781 >5k 1.3904764819717597E-4 0.6074824646666168 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8656 0.36992385705451547 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5554 0.23735641197790885 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3744 0.16000403428975346 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2877 0.12295181801592434 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.2736120269699106E-5 0.0 0.0 0.0 0.0 3 4.2736120269699106E-5 0.0 0.0 0.0 0.0 4 4.2736120269699106E-5 0.0 0.0 0.0 0.0 5 1.2820836080909732E-4 0.0 0.0 0.0 0.0 6 1.7094448107879642E-4 0.0 0.0 0.0 0.0 7 2.1368060134849555E-4 0.0 0.0 0.0 0.0 8 2.1368060134849555E-4 0.0 0.0 0.0 0.0 9 2.1368060134849555E-4 0.0 0.0 4.2736120269699106E-5 0.0 10-11 2.5641672161819465E-4 0.0 0.0 4.2736120269699106E-5 0.0 12-13 2.991528418878937E-4 0.0 0.0 4.2736120269699106E-5 0.0 14-15 4.273612026969911E-4 0.0 0.0 4.2736120269699106E-5 2.1368060134849553E-5 16-17 4.273612026969911E-4 0.0 0.0 6.410418040454866E-5 1.2820836080909732E-4 18-19 5.983056837757874E-4 0.0 0.0 8.547224053939821E-5 1.2820836080909732E-4 20-21 6.410418040454866E-4 0.0 0.0 1.2820836080909732E-4 1.2820836080909732E-4 22-23 6.410418040454866E-4 0.0 0.0 2.777847817530442E-4 1.2820836080909732E-4 24-25 7.265140445848849E-4 0.0 0.0 3.8462508242729197E-4 1.2820836080909732E-4 26-27 7.265140445848849E-4 0.0 0.0 5.76937623640938E-4 1.2820836080909732E-4 28-29 9.401946459333803E-4 0.0 0.0 0.001495764209439469 1.2820836080909732E-4 30-31 9.829307662030794E-4 0.0 0.0 0.003312049320901681 1.2820836080909732E-4 32-33 0.001004298826337929 0.0 0.0 0.005213806672903292 1.2820836080909732E-4 34-35 0.0011752433074167256 0.0 0.0 0.006901883423556406 1.7094448107879642E-4 36-37 0.0013248197283606724 0.0 0.0 0.008760904655288317 1.7094448107879642E-4 38-39 0.0014957642094394687 0.0 0.0 0.01252168323902184 1.7094448107879642E-4 40-41 0.0014957642094394687 0.0 0.0 0.01737223288963269 1.7094448107879642E-4 42-43 0.001688076750653115 0.0 0.0 0.021859525517951093 1.7094448107879642E-4 44-45 0.0018376531715970617 0.0 0.0 0.024188644072649694 1.7094448107879642E-4 46-47 0.002051333772945557 0.0 0.0 0.02775711011516957 1.7094448107879642E-4 48-49 0.002094069893215256 0.0 0.0 0.03218029856308342 1.7094448107879642E-4 50-51 0.002094069893215256 0.0 0.0 0.037757362258279165 1.7094448107879642E-4 52-53 0.002094069893215256 0.0 0.0 0.048505496506108484 1.7094448107879642E-4 54-55 0.002094069893215256 0.0 0.0 0.05542874798979974 1.7094448107879642E-4 56-57 0.002307750494563752 0.0 0.0 0.07538651615574923 2.350486614833451E-4 58-59 0.002307750494563752 0.0 0.0 0.11337892707551173 2.991528418878938E-4 60-61 0.002307750494563752 0.0 0.0 0.14308053066295262 2.991528418878938E-4 62-63 0.002307750494563752 0.0 0.0 0.15801680469721247 2.991528418878938E-4 64-65 0.002307750494563752 0.0 0.0 0.1887868112913958 2.991528418878938E-4 66-67 0.00239322273510315 0.0 0.0 0.25556199921280065 2.991528418878938E-4 68-69 0.00239322273510315 0.0 0.0 0.3546670621182329 2.991528418878938E-4 70-71 0.00239322273510315 0.0 0.0 0.4381093369448204 2.991528418878938E-4 72-73 0.002500063035777398 0.0 0.0 0.5399281434873785 3.205209020227433E-4 74-75 0.002628271396586495 0.0 0.0 0.6547173625317904 3.4188896215759285E-4 76-77 0.0026710075168561945 0.0 0.0 0.7288645311997183 3.4188896215759285E-4 78-79 0.0027351116972607428 0.0 0.0 0.834294539905066 3.4188896215759285E-4 80-81 0.003012896479013787 0.0 0.0 0.9533360029163128 3.4188896215759285E-4 82-83 0.0030770006594183358 0.0 0.0 1.0361799720591245 3.4188896215759285E-4 84-85 0.0031411048398228844 0.0 0.0 1.1813759406754274 3.4188896215759285E-4 86-87 0.0032693132006319818 0.0 0.0 1.416488706339177 3.4188896215759285E-4 88-89 0.0032906812607668315 0.0 0.0 1.727543557722182 3.4188896215759285E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12050 0.0 31.582804 1 GGTATCA 8140 0.0 29.067665 1 ATCAACG 14355 0.0 26.048698 3 TATCAAC 14525 0.0 25.84196 2 CAACGCA 14590 0.0 25.629683 5 TCAACGC 14590 0.0 25.564548 4 GTACATG 9425 0.0 25.356647 1 AACGCAG 15130 0.0 24.809683 6 TACATGG 9410 0.0 24.791704 2 GTATACG 135 1.3408289E-5 24.635962 1 ACATGGG 9690 0.0 22.751429 3 ACGCAGA 16940 0.0 21.8774 7 CGCAGAG 16810 0.0 21.876999 8 GAGTACT 7975 0.0 21.832195 12-13 AGAGTAC 12780 0.0 20.89191 10-11 GTATAGG 570 0.0 20.838688 1 CATGGGG 4715 0.0 20.557592 4 GCAGAGT 17435 0.0 20.328415 9 GTACTTT 8525 0.0 20.115236 14-15 AGTACTT 8665 0.0 19.846966 12-13 >>END_MODULE