FastQCFastQC Report
Thu 26 May 2016
SRR937057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339941
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94090.40210415561759894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68770.29389629909472076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45380.19393651378389457No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39300.1679529526599175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA138950.030.2962281
GGTATCA95050.025.393641
TATCAAC166850.025.0228542
ATCAACG166450.024.9952433
TCAACGC167150.024.8905664
CAACGCA168050.024.763615
AACGCAG173550.024.0335736
GTACATG96400.022.672231
TACATGG96750.022.0920332
GAGTACT93850.021.38152912-13
ACGCAGA196350.021.0492557
AGTACTT97850.020.9685912-13
ACATGGG97750.020.9410063
CGCAGAG196550.020.9069888
AGAGTAC143900.020.89735610-11
GTACTTT98750.020.1281914-15
GCAGAGT204800.019.6008639
CCTATAC5650.019.3337653
TGTACCG2803.4877303E-818.663075
CATGGGG52200.018.1968634
Produced by FastQC (version 0.11.5)