##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937057_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2339941 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25656373387192 33.0 31.0 34.0 31.0 34.0 2 32.391839794251226 34.0 31.0 34.0 31.0 34.0 3 32.50417681471456 34.0 31.0 34.0 31.0 34.0 4 36.12726944824677 37.0 35.0 37.0 35.0 37.0 5 36.03617441636349 37.0 35.0 37.0 35.0 37.0 6 36.07628269259781 37.0 35.0 37.0 35.0 37.0 7 36.0280314760073 37.0 35.0 37.0 35.0 37.0 8 35.990645063272964 37.0 35.0 37.0 35.0 37.0 9 37.69092041209586 39.0 37.0 39.0 35.0 39.0 10-11 37.60791917403046 39.0 37.0 39.0 35.0 39.0 12-13 37.51672542170935 39.0 37.0 39.0 35.0 39.0 14-15 38.75182536653702 40.0 38.0 41.0 35.0 41.0 16-17 38.67098828560207 40.0 38.0 41.0 34.0 41.0 18-19 38.81600796772226 40.0 38.0 41.0 35.0 41.0 20-21 38.840228663885114 40.0 38.0 41.0 35.0 41.0 22-23 38.76180083173037 40.0 38.0 41.0 34.5 41.0 24-25 38.66106239430823 40.0 38.0 41.0 34.0 41.0 26-27 38.493551760493105 40.0 38.0 41.0 34.0 41.0 28-29 38.41585471599498 40.0 38.0 41.0 34.0 41.0 30-31 38.41317558861527 40.0 38.0 41.0 34.0 41.0 32-33 38.26264978475953 40.0 38.0 41.0 34.0 41.0 34-35 38.119428865941494 40.0 38.0 41.0 34.0 41.0 36-37 37.969356492321815 40.0 37.0 41.0 33.0 41.0 38-39 37.817656086200465 40.0 37.0 41.0 33.0 41.0 40-41 37.64370362329649 40.0 37.0 41.0 33.0 41.0 42-43 37.397493355601696 40.0 37.0 41.0 32.5 41.0 44-45 37.13773595146203 40.0 37.0 41.0 31.5 41.0 46-47 36.79387493103458 39.5 36.0 41.0 31.0 41.0 48-49 36.43931513657823 39.0 36.0 41.0 30.0 41.0 50-51 36.14918730856889 39.0 35.5 40.5 30.0 41.0 52-53 35.909879351658866 39.0 35.0 40.0 29.0 41.0 54-55 35.62223684272381 39.0 35.0 40.0 28.0 41.0 56-57 35.333068226933925 38.0 35.0 40.0 28.0 41.0 58-59 34.97803085633356 38.0 34.0 40.0 27.0 41.0 60-61 34.639720403206745 38.0 34.0 40.0 26.0 41.0 62-63 34.27430392475708 37.0 34.0 40.0 26.0 41.0 64-65 33.88527082520457 36.5 33.0 39.0 26.0 40.5 66-67 33.48460238954743 36.0 33.0 39.0 25.0 40.0 68-69 33.17066434581043 36.0 32.5 39.0 24.5 40.0 70-71 32.70009136982514 35.0 32.0 38.0 23.5 40.0 72-73 32.20036466731426 35.0 31.5 37.5 22.0 39.5 74-75 31.69318649487316 35.0 31.0 37.0 20.5 39.0 76-77 30.289229087400066 33.5 29.5 35.5 20.0 37.5 78-79 31.02411278745917 34.0 31.0 36.0 20.0 38.0 80-81 30.78870898881638 34.0 31.0 35.5 18.0 37.0 82-83 30.468722715658217 34.0 30.5 35.0 17.5 37.0 84-85 30.114900546637713 34.0 30.0 35.0 15.5 36.5 86-87 29.76928948208523 34.0 30.0 35.0 9.5 36.0 88-89 29.379662991502776 34.0 29.5 35.0 7.0 36.0 90-91 28.929131760159763 34.0 29.0 35.0 2.0 35.0 92-93 28.45126287372203 33.5 29.0 35.0 2.0 35.0 94-95 27.87008646799214 33.0 27.5 35.0 2.0 35.0 96-97 27.213945778974768 33.0 26.5 34.5 2.0 35.0 98-99 26.39942460087669 32.5 25.0 34.0 2.0 35.0 100-101 22.875652420296067 28.5 11.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10422141345144098 1101 2 0.09124262830329144 1101 3 0.13144047312163565 1101 4 0.10616887765014127 1101 5 0.1468706455063753 1101 6 0.16767714008582146 1101 7 0.1097532394721128 1101 8 0.17681735503081342 1101 9 0.19990643055528068 1101 10-11 0.21163297074111398 1101 12-13 0.23102921513559238 1101 14-15 0.282002085071241 1101 16-17 0.305674384524913 1101 18-19 0.17830989131426378 1101 20-21 0.270512471517236 1101 22-23 0.26689283669343666 1101 24-25 0.21172128266977097 1101 26-27 0.25371038895789866 1101 28-29 0.23366706531485448 1101 30-31 0.2642814623459273 1101 32-33 0.36008983301181985 1101 34-35 0.2933676167986903 1101 36-37 0.30841913365078 1101 38-39 0.24077470100594667 1101 40-41 0.27916791790052997 1101 42-43 0.3985587847217502 1101 44-45 0.3843161240162374 1101 46-47 0.13614991524817555 1101 48-49 0.28112850903463027 1101 50-51 0.09150353394269928 1101 52-53 0.026307604039651267 1101 54-55 0.16124949069144634 1101 56-57 0.2221676758388469 1101 58-59 0.06954513330176582 1101 60-61 0.17332523753508156 1101 62-63 0.1967877942001124 1101 64-65 0.1621607109037626 1101 66-67 0.004721973218387632 1101 68-69 -0.05288089389951267 1101 70-71 -0.0969607241395849 1101 72-73 0.13268153502779256 1101 74-75 0.1129330722762063 1101 76-77 -0.01145198392526936 1101 78-79 0.023270822754312803 1101 80-81 0.22855421838163892 1101 82-83 0.22085314391663857 1101 84-85 0.2116524552072434 1101 86-87 -0.1261786840046426 1101 88-89 -0.222533309997754 1101 90-91 0.09701588662459315 1101 92-93 0.1993241823688301 1101 94-95 0.026944014840637465 1101 96-97 0.015181086948583555 1101 98-99 0.20849177383552941 1101 100-101 0.6187677406678489 1102 1 0.18790259789641084 1102 2 0.20786490277563274 1102 3 0.19623446272468215 1102 4 0.10212692243421628 1102 5 0.17252357814658126 1102 6 0.0902965857038538 1102 7 0.07434039206492571 1102 8 0.12532779791183657 1102 9 0.17154661498189228 1102 10-11 0.2037408345887215 1102 12-13 0.19917007003741105 1102 14-15 0.2817063412111338 1102 16-17 0.24149025182592965 1102 18-19 0.1484103707499571 1102 20-21 0.20543375956440002 1102 22-23 0.19407922880203898 1102 24-25 0.28579795564319 1102 26-27 0.21306893705760643 1102 28-29 0.25540368795670787 1102 30-31 0.2443237114687946 1102 32-33 0.2269316192312587 1102 34-35 0.1606480591286399 1102 36-37 0.2648184062310932 1102 38-39 0.40593276783304333 1102 40-41 0.3383258745125133 1102 42-43 0.24826653100658547 1102 44-45 0.2859090996403353 1102 46-47 0.26535177420957723 1102 48-49 0.4281198204106573 1102 50-51 0.3692683716509606 1102 52-53 0.19488674766829917 1102 54-55 0.21573102047707238 1102 56-57 0.1507365327245367 1102 58-59 0.22760644963747723 1102 60-61 0.18400030504183462 1102 62-63 0.29408789155679216 1102 64-65 0.1848301207018892 1102 66-67 0.15342896636668257 1102 68-69 0.05034620432051895 1102 70-71 0.024408882208803107 1102 72-73 0.2205046030488731 1102 74-75 0.16823754149863746 1102 76-77 0.14165553090784755 1102 78-79 0.02837525156454035 1102 80-81 0.28539324922349607 1102 82-83 0.24568350450372023 1102 84-85 0.2600905235038695 1102 86-87 -0.01412592770860499 1102 88-89 0.19403863291082502 1102 90-91 0.23905853986951442 1102 92-93 0.05184608762058218 1102 94-95 0.033970227665999886 1102 96-97 0.1461092818088865 1102 98-99 0.26634845746307434 1102 100-101 0.5156909023717731 1103 1 0.22183051242699747 1103 2 0.26522020352309994 1103 3 0.24941256314341587 1103 4 0.1629487437467816 1103 5 0.13932043174498432 1103 6 0.1729929422960339 1103 7 0.16782374039975423 1103 8 0.07730134309244363 1103 9 0.29039606514429295 1103 10-11 0.2438625424116765 1103 12-13 0.25271994676420917 1103 14-15 0.34865934085893 1103 16-17 0.3393276877434275 1103 18-19 0.3536426331066309 1103 20-21 0.32154497938203974 1103 22-23 0.222418367063689 1103 24-25 0.24618929912536203 1103 26-27 0.36642314204332394 1103 28-29 0.257245350383819 1103 30-31 0.2971926505366085 1103 32-33 0.28585816405965403 1103 34-35 0.30457597365921885 1103 36-37 0.2276294264635581 1103 38-39 0.26106993372354026 1103 40-41 0.24097722660425092 1103 42-43 0.2836075868512964 1103 44-45 0.2371030886044423 1103 46-47 0.04926599171827917 1103 48-49 -0.0686093307653266 1103 50-51 0.030166113599065625 1103 52-53 0.11035616417516536 1103 54-55 0.18882067726884344 1103 56-57 0.1571479129929969 1103 58-59 0.15095595850140597 1103 60-61 0.0383450003809358 1103 62-63 0.052478687158092896 1103 64-65 -0.05500473234863534 1103 66-67 -0.014790812132908115 1103 68-69 -0.13344473999130457 1103 70-71 -0.25858450408634326 1103 72-73 -0.18524618942166882 1103 74-75 0.031385117125765305 1103 76-77 -0.2024054404066753 1103 78-79 -0.17742289346735518 1103 80-81 -0.025019226806712425 1103 82-83 0.07317225337860478 1103 84-85 -0.026360773799776638 1103 86-87 -0.03750400464673476 1103 88-89 -0.16971004630492814 1103 90-91 -0.21596709312857243 1103 92-93 -0.09809051473530772 1103 94-95 -0.1394712469162407 1103 96-97 -0.034347024691331995 1103 98-99 -0.12992477855618034 1103 100-101 0.10200562080013853 1104 1 0.16156380576124718 1104 2 0.14841978991554328 1104 3 0.16886679903057455 1104 4 0.020479608732316024 1104 5 0.08113803910821815 1104 6 0.010954999546846977 1104 7 0.09964375728235098 1104 8 0.09015078323822223 1104 9 0.14949758569462546 1104 10-11 0.20023819445793123 1104 12-13 0.08745452464354742 1104 14-15 0.059480077405908105 1104 16-17 0.1512295963699799 1104 18-19 0.08511558345936265 1104 20-21 0.14983856096500858 1104 22-23 0.09197951539912452 1104 24-25 0.08322026065124533 1104 26-27 -0.03673604101791739 1104 28-29 0.004927185320575234 1104 30-31 0.0994384502337482 1104 32-33 0.03100593461596901 1104 34-35 -0.004842933248760062 1104 36-37 -0.029914762685315566 1104 38-39 -0.01589665693422404 1104 40-41 -0.0415675609351851 1104 42-43 -0.18102840817646637 1104 44-45 -0.18946417608045607 1104 46-47 -0.055241086045363375 1104 48-49 -0.2545375781511581 1104 50-51 -0.11889648762867466 1104 52-53 -0.0598654477773195 1104 54-55 -0.13299791943347117 1104 56-57 -0.14806023916096933 1104 58-59 -0.1581880078866007 1104 60-61 -0.06631215373734989 1104 62-63 0.004666961575217954 1104 64-65 -0.04055990316121694 1104 66-67 0.02574304799689031 1104 68-69 -0.0687436400696555 1104 70-71 -0.1966878612967946 1104 72-73 -0.013578865855137678 1104 74-75 0.030640320331226434 1104 76-77 -0.13026498936380193 1104 78-79 -0.06442840344771028 1104 80-81 0.30024921120242 1104 82-83 0.20052939361422872 1104 84-85 0.23002556949130692 1104 86-87 -0.05368719832900837 1104 88-89 0.04475640472837483 1104 90-91 0.08316938938805052 1104 92-93 -0.13064878600888719 1104 94-95 0.0010055666460040413 1104 96-97 -0.10018754004124375 1104 98-99 0.07134819764702627 1104 100-101 0.058722036328934024 1105 1 0.18043773674474295 1105 2 0.22624035062370496 1105 3 0.15454022932128453 1105 4 0.15715421494327586 1105 5 0.16325621850658933 1105 6 0.2155005493906259 1105 7 0.17176300844371895 1105 8 0.10080082007903002 1105 9 0.21132103529886592 1105 10-11 0.1620761355056004 1105 12-13 0.14850283297717937 1105 14-15 0.2368664147589925 1105 16-17 0.2003695107530632 1105 18-19 0.22522469530662903 1105 20-21 0.23851696983806647 1105 22-23 0.32092872305764786 1105 24-25 0.206094450602194 1105 26-27 0.2736950811011738 1105 28-29 0.23851046809878795 1105 30-31 0.25616475265642435 1105 32-33 0.14093820892222197 1105 34-35 0.2224062972706875 1105 36-37 0.22604798826699124 1105 38-39 0.2560242090502811 1105 40-41 0.30346632707044563 1105 42-43 0.38530692885609596 1105 44-45 0.24071147061277998 1105 46-47 0.17609124283861632 1105 48-49 0.07714522897560983 1105 50-51 0.20612654521239193 1105 52-53 0.1654110846886212 1105 54-55 0.30915136625074524 1105 56-57 0.2825559373488318 1105 58-59 0.0844188820232219 1105 60-61 -0.005411024125983488 1105 62-63 0.18270541388235273 1105 64-65 0.017052557735787843 1105 66-67 -0.01314081566345493 1105 68-69 -0.08451474168268192 1105 70-71 -0.2574901169973032 1105 72-73 -0.03484715564173513 1105 74-75 0.18941932565129704 1105 76-77 -0.018463922666093424 1105 78-79 0.1534253735272948 1105 80-81 0.30870364341206624 1105 82-83 0.33646114562470686 1105 84-85 0.4151109054755935 1105 86-87 0.11572792265358345 1105 88-89 0.04601459411308895 1105 90-91 0.15840648131093715 1105 92-93 0.016829325454128963 1105 94-95 -0.076065682447652 1105 96-97 -0.035367598741654405 1105 98-99 -0.22181839910625456 1105 100-101 0.10909380726550566 1106 1 0.13004970686713335 1106 2 0.07202947201633236 1106 3 0.10944606700984139 1106 4 0.08988046170700414 1106 5 0.09214582781220315 1106 6 0.09563705242207732 1106 7 0.13530912510568527 1106 8 0.0977439898978858 1106 9 0.13025747786792152 1106 10-11 0.11982650357857949 1106 12-13 0.14365974386452507 1106 14-15 0.26445588504769546 1106 16-17 0.2002755537484333 1106 18-19 0.034303703285623044 1106 20-21 0.1900804305327526 1106 22-23 0.009470845650383808 1106 24-25 0.06090882365704431 1106 26-27 0.09510460113329344 1106 28-29 0.1712296322257174 1106 30-31 0.1810306173773384 1106 32-33 0.1624081017090262 1106 34-35 0.12446974729659388 1106 36-37 0.17990936116171952 1106 38-39 0.19972800493119536 1106 40-41 0.14706583784850835 1106 42-43 0.10796638187212437 1106 44-45 0.0413407135829118 1106 46-47 0.044461155151985565 1106 48-49 -0.03292907774631715 1106 50-51 -0.1374064425719439 1106 52-53 -0.1078431394766497 1106 54-55 -0.19412663943651864 1106 56-57 -0.06017626420901934 1106 58-59 0.07223782257192823 1106 60-61 -0.020475523637280446 1106 62-63 0.07517789783837969 1106 64-65 -0.026763054152922905 1106 66-67 0.03551582330442926 1106 68-69 0.023470635451587896 1106 70-71 -0.10766365952962076 1106 72-73 -0.10264627915199043 1106 74-75 -0.09169169441157266 1106 76-77 -0.036752277071911976 1106 78-79 0.11428080543455721 1106 80-81 -0.013574261683828581 1106 82-83 0.06081693498099128 1106 84-85 0.09916093291856853 1106 86-87 0.058250352647092996 1106 88-89 0.20971450261259506 1106 90-91 0.2512457741302718 1106 92-93 0.2326089381827181 1106 94-95 0.24897597511170133 1106 96-97 0.44786385519457994 1106 98-99 -0.00587185131790946 1106 100-101 0.08778780131501307 1107 1 0.1282380259346283 1107 2 0.17343356973407253 1107 3 0.11861515950995738 1107 4 0.10690929057928855 1107 5 0.15396669493532045 1107 6 0.16859797547585487 1107 7 0.15789880295188397 1107 8 0.17615183444392812 1107 9 0.23055271742699546 1107 10-11 0.2114134000104002 1107 12-13 0.15220502490904408 1107 14-15 0.22093333730524023 1107 16-17 0.12913441073722964 1107 18-19 0.0904305360692419 1107 20-21 0.12414134591936943 1107 22-23 0.19469236351000063 1107 24-25 0.10174102701454046 1107 26-27 0.15771117192329598 1107 28-29 0.2691030578937017 1107 30-31 0.2530127179841273 1107 32-33 0.2752478728749779 1107 34-35 0.21573744880155488 1107 36-37 0.21108121599831264 1107 38-39 0.282486331446286 1107 40-41 0.2766990237540483 1107 42-43 0.28513603447069613 1107 44-45 0.2635037123126125 1107 46-47 0.20217602544203572 1107 48-49 0.3021017690642367 1107 50-51 0.2246433953918654 1107 52-53 0.3209102491010114 1107 54-55 0.32028167737762914 1107 56-57 0.3091568334702046 1107 58-59 0.28974734262585855 1107 60-61 0.2510566804041403 1107 62-63 0.432908030451415 1107 64-65 0.2518306739682288 1107 66-67 0.4214187102952067 1107 68-69 0.5007939371671952 1107 70-71 0.5407645911741952 1107 72-73 0.35814322929682163 1107 74-75 0.3909691908103561 1107 76-77 0.5449691833740111 1107 78-79 0.5941802540831382 1107 80-81 0.8605502695126859 1107 82-83 0.7097026954136361 1107 84-85 0.7307882997035087 1107 86-87 0.6854662241671292 1107 88-89 0.7696089514105608 1107 90-91 0.8306587182359699 1107 92-93 0.8664524014629755 1107 94-95 0.7382638221630664 1107 96-97 0.7295050984547693 1107 98-99 0.6191469116204189 1107 100-101 0.8479442653227487 1108 1 0.1129575965126719 1108 2 0.08521654282073854 1108 3 0.05837914027128477 1108 4 0.11534856499897472 1108 5 0.1769880696520545 1108 6 0.09652202426773471 1108 7 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-0.877020499499352 2316 46-47 -1.0329492974706227 2316 48-49 -1.0362126971107557 2316 50-51 -0.869751356164528 2316 52-53 -0.8869517957366 2316 54-55 -0.8991236166720356 2316 56-57 -0.992605051433884 2316 58-59 -0.9240682824424695 2316 60-61 -0.816553890827187 2316 62-63 -0.8713586727625611 2316 64-65 -0.7585437251096607 2316 66-67 -0.8323519881332615 2316 68-69 -1.0011019545403173 2316 70-71 -0.9064337217145066 2316 72-73 -0.6709038776868361 2316 74-75 -0.8071322623356778 2316 76-77 -0.5979993250271818 2316 78-79 -0.8479486813905517 2316 80-81 -0.6866435921010847 2316 82-83 -0.5077436472002148 2316 84-85 -0.5926928784318299 2316 86-87 -0.5154366037200262 2316 88-89 -0.679740615800565 2316 90-91 -0.5939866635272324 2316 92-93 -0.5478630939717348 2316 94-95 -0.47278107948718784 2316 96-97 -0.2382836799592063 2316 98-99 -0.5578808783918561 2316 100-101 -0.9043331084562745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 8.0 4 16.0 5 46.0 6 82.0 7 195.0 8 519.0 9 1089.0 10 1839.0 11 2998.0 12 4689.0 13 6818.0 14 8799.0 15 12247.0 16 16074.0 17 22180.0 18 20330.0 19 12748.0 20 11170.0 21 11647.0 22 12108.0 23 13498.0 24 15584.0 25 18542.0 26 22500.0 27 27893.0 28 34890.0 29 43578.0 30 54935.0 31 70002.0 32 89014.0 33 117325.0 34 161934.0 35 232436.0 36 363658.0 37 536318.0 38 368422.0 39 23803.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.138608591941555 19.452354031253137 14.845861021636953 22.563176355168352 2 15.60619503822536 20.97620830427112 39.73642281759871 23.68117383990481 3 19.90056129788276 24.886471156878784 29.256167054284436 25.95680049095402 4 13.260037787406734 17.129678499341647 36.84434554322709 32.765938170024526 5 14.110313037807366 37.344616808714406 33.67426785547157 14.870802298006659 6 31.837170253438014 36.30249651593779 17.399327589883676 14.461005640740515 7 28.581618083532877 32.61351461425737 20.609451263942123 18.19541603826763 8 26.2617732669328 34.74232897325189 19.66857284008443 19.32732491973088 9 26.328783503515684 17.31056466808351 20.417309667209558 35.94334216119125 10-11 24.873019967392413 26.972681047657126 27.056476008376507 21.097822976573955 12-13 26.038241989862136 24.240547090717243 27.788029698184697 21.93318122123592 14-15 22.96814321386736 25.52944710999123 26.340471832409452 25.16193784373196 16-17 22.051090177060022 28.785960842602442 26.774734918529997 22.388214061807542 18-19 21.863909264010505 27.37639969375172 29.445114098425528 21.314576943812245 20-21 22.83799628063969 26.33215644049141 29.54739662941252 21.28245064945638 22-23 22.427416614848102 26.265772609454697 29.46219809008524 21.84461268561196 24-25 22.30833342237704 26.387553826920406 29.60579995084787 21.69831279985468 26-27 22.176124194142243 26.512824381186505 29.600668085436467 21.710383339234784 28-29 21.925962932319518 26.808297320952367 29.564868440846247 21.70087130588187 30-31 22.141472454095858 26.49909653332057 29.459445714217335 21.899985298366236 32-33 22.129921970743567 26.609312011248694 29.440076244936858 21.820689773070878 34-35 22.481464276236025 26.584324134668353 29.30721330153196 21.626998287563662 36-37 22.491969206887592 26.406585506790485 29.342761430310325 21.758683856011597 38-39 22.19351664818815 26.772356083420885 29.298926251488467 21.7352010169025 40-41 22.4081695449825 26.68181682201452 29.128812646640256 21.781200986362723 42-43 22.19870073647156 26.774243453146894 28.904532208290725 22.122523602090823 44-45 22.377209510838096 26.855057456576898 28.768887762554694 21.998845270030312 46-47 22.74801340550631 27.11106381632986 28.223872981032798 21.917049797131032 48-49 22.70774457900838 27.2169625351917 28.062738729305675 22.01255415649424 50-51 22.489407060364154 27.483923606035816 28.034384635044702 21.992284698555327 52-53 22.78339496594145 27.53496776200767 27.6254828647389 22.05615440731198 54-55 22.644511153259987 27.44818693815939 27.648648163517898 22.258653745062727 56-57 22.645620751706367 27.695802676810967 27.60620239918473 22.052374172297938 58-59 22.89672576374985 27.311185994482752 27.612155893219253 22.179932348548142 60-61 22.697232336701028 27.445339077217636 27.974596987879718 21.882831598201616 62-63 22.443192442113293 27.4565535840021 28.110325315223584 21.98992865866102 64-65 22.523398035847073 27.470041966888044 27.868727403266753 22.137832593998137 66-67 22.560972873521955 27.553766284274033 27.627295931722774 22.257964910481235 68-69 22.501922066866133 27.466474410120988 27.838515613080567 22.19308790993231 70-71 22.62135436877437 27.431211441055353 27.67980925876225 22.26762493140803 72-73 22.6192241599254 27.398105336843965 27.67826197327198 22.30440852995866 74-75 22.49584978763349 27.824083561099094 27.398282135806447 22.28178451546097 76-77 22.67279388668347 27.57447730519701 27.4479142850183 22.304814523101225 78-79 22.65405638726607 27.576809166242853 27.391266256106057 22.377868190385016 80-81 22.746039265764352 27.740193869289197 27.246577862796546 22.2671890021499 82-83 22.677789251606242 27.690154486361912 27.40607037712043 22.225985884911424 84-85 22.6137631265146 27.708410799536697 27.251798284417433 22.426027789531272 86-87 22.49738976428599 27.645270355457903 27.47474492772329 22.382594952532816 88-89 22.62044977260081 27.680754789229457 27.116573174161022 22.582222264008706 90-91 22.5570911490369 27.651153105415332 27.225155726444548 22.566600019103213 92-93 22.577965190110767 27.621153290994616 27.2231300096135 22.577751509281118 94-95 22.5339917831244 27.54903383227052 27.207144797057875 22.709829587547205 96-97 22.35158766485333 27.623517928033152 27.374274696640814 22.650619710472704 98-99 22.195228950501168 27.828329914715837 27.022734251512947 22.95370688327004 100-101 22.093424529735163 28.06103512004518 26.914441726178733 22.931098624040917 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 392.0 1 293.5 2 228.5 3 280.0 4 272.5 5 277.5 6 465.5 7 934.0 8 1558.0 9 1950.5 10 1919.5 11 1836.0 12 1999.5 13 2314.0 14 2589.5 15 2881.5 16 3144.5 17 3304.0 18 3525.0 19 3881.5 20 4321.5 21 4937.0 22 5798.5 23 6995.0 24 8449.5 25 10311.5 26 13211.5 27 16923.5 28 20345.0 29 23814.0 30 27928.0 31 32495.0 32 36717.5 33 41252.5 34 46488.5 35 51246.0 36 57189.0 37 62509.5 38 65627.0 39 68147.5 40 69991.5 41 72529.5 42 75044.5 43 77805.5 44 81682.0 45 84244.5 46 85210.0 47 88154.0 48 94190.5 49 93501.5 50 87188.5 51 83764.5 52 81540.0 53 80032.5 54 77918.5 55 72835.5 56 65363.0 57 58421.0 58 53883.5 59 48727.0 60 40898.0 61 32722.0 62 25699.5 63 19846.5 64 14510.0 65 10201.5 66 7264.0 67 5492.0 68 4106.0 69 2767.0 70 1976.5 71 1332.5 72 872.5 73 618.0 74 413.0 75 266.5 76 175.5 77 78.0 78 40.0 79 27.0 80 18.0 81 12.0 82 5.0 83 3.0 84 3.0 85 2.5 86 1.5 87 1.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028034894896922614 2 0.005683903995869981 3 2.991528418878938E-4 4 3.4188896215759285E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01288494026131428 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.0599314256214156E-4 20-21 9.615627060682299E-4 22-23 0.001965861532406159 24-25 0.011367807991739962 26-27 0.005149702492498742 28-29 2.1368060134849552E-4 30-31 0.002777847817530442 32-33 0.004850549650610849 34-35 0.0 36-37 0.008419015693130725 38-39 0.012265266517403643 40-41 0.0013248197283606724 42-43 0.0 44-45 0.0 46-47 2.991528418878938E-4 48-49 0.00335478544117138 50-51 0.007478821047197344 52-53 0.0 54-55 0.0037180424634638224 56-57 0.004465924568183557 58-59 2.5641672161819465E-4 60-61 0.0026069033364516454 62-63 0.005705272056004831 64-65 4.2736120269699106E-5 66-67 0.0029274242384743888 68-69 5.128334432363893E-4 70-71 7.265140445848849E-4 72-73 0.0 74-75 2.1368060134849553E-5 76-77 0.0 78-79 0.0021795421337546545 80-81 0.002799215877665292 82-83 0.013547350125494618 84-85 0.009017321376906511 86-87 0.004871917710745698 88-89 1.2820836080909732E-4 90-91 8.760904655288317E-4 92-93 1.0684030067424776E-4 94-95 2.1368060134849553E-5 96-97 0.002478694975642548 98-99 0.002457326915507699 100-101 4.0599314256214156E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2339941.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.15737485061153 #Duplication Level Percentage of deduplicated Percentage of total 1 79.32019483154946 46.92374498873511 2 12.06552268051778 14.275292959598913 3 3.6486805990007065 6.47539097715716 4 1.5998969644714196 3.7858281779836522 5 0.8501798994116112 2.514720549997395 6 0.5280644271543176 1.874334315746485 7 0.3601554552353152 1.491409588889373 8 0.2606968908487849 1.2337714955464423 9 0.1865098147920405 0.9930087924273787 >10 1.0640479998311323 11.58707346835419 >50 0.07884120368016408 3.2105574739709306 >100 0.035257135498064626 3.7228868930552363 >500 0.00122909874648226 0.5077314355048518 >1k 5.78399410108761E-4 0.7076846375080044 >5k 1.4459985252719026E-4 0.696564245524922 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9409 0.40210415561759894 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6877 0.29389629909472076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4538 0.19393651378389457 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3930 0.1679529526599175 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.2736120269699106E-5 0.0 0.0 0.0 0.0 6 8.547224053939821E-5 0.0 0.0 0.0 0.0 7 2.5641672161819465E-4 0.0 0.0 0.0 0.0 8 2.5641672161819465E-4 0.0 0.0 0.0 0.0 9 2.5641672161819465E-4 0.0 0.0 0.0 0.0 10-11 2.5641672161819465E-4 0.0 0.0 0.0 0.0 12-13 3.205209020227433E-4 0.0 0.0 0.0 0.0 14-15 3.8462508242729197E-4 0.0 0.0 0.0 2.1368060134849553E-5 16-17 3.8462508242729197E-4 0.0 0.0 0.0 4.2736120269699106E-5 18-19 5.342015033712389E-4 0.0 0.0 0.0 4.2736120269699106E-5 20-21 6.410418040454866E-4 0.0 0.0 4.2736120269699106E-5 8.547224053939821E-5 22-23 7.265140445848849E-4 0.0 0.0 1.9231254121364599E-4 8.547224053939821E-5 24-25 8.547224053939822E-4 0.0 0.0 3.4188896215759285E-4 8.547224053939821E-5 26-27 8.547224053939822E-4 0.0 0.0 5.555695635060884E-4 8.547224053939821E-5 28-29 0.0011325071871470264 0.0 0.0 0.0015171322695743182 8.547224053939821E-5 30-31 0.001196611367551575 0.0 0.0 0.0033761535013062294 8.547224053939821E-5 32-33 0.001196611367551575 0.0 0.0 0.005299278913442689 8.547224053939821E-5 34-35 0.0012820836080909732 0.0 0.0 0.007051459844500353 8.547224053939821E-5 36-37 0.0014743961493046192 0.0 0.0 0.00903868943704136 8.547224053939821E-5 38-39 0.001623972570248566 0.0 0.0 0.012970412501853679 1.2820836080909732E-4 40-41 0.001623972570248566 0.0 0.0 0.01773548991192513 1.7094448107879642E-4 42-43 0.0017308128709228139 0.0 0.0 0.022158678359838986 1.7094448107879642E-4 44-45 0.0017949170513273625 0.0 0.0 0.02453053303480729 1.7094448107879642E-4 46-47 0.002136806013484955 0.0 0.0 0.028163103257731713 1.7094448107879642E-4 48-49 0.0022222782540243535 0.0 0.0 0.03262902782591527 1.7094448107879642E-4 50-51 0.0022222782540243535 0.0 0.0 0.038035147040032205 1.7094448107879642E-4 52-53 0.0022222782540243535 0.0 0.0 0.048633704866917586 1.7094448107879642E-4 54-55 0.002243646314159203 0.0 0.0 0.05549285217020429 1.7094448107879642E-4 56-57 0.002265014374294053 0.0 0.0 0.07534378003547954 1.7094448107879642E-4 58-59 0.002265014374294053 0.0 0.0 0.11361397573699508 1.7094448107879642E-4 60-61 0.002265014374294053 0.0 0.0 0.14352925992578444 1.7094448107879642E-4 62-63 0.002265014374294053 0.0 0.0 0.1583800617195049 1.7094448107879642E-4 64-65 0.002265014374294053 0.0 0.0 0.18863723487045186 1.7094448107879642E-4 66-67 0.002350486614833451 0.0 0.0 0.25500642964929454 1.7094448107879642E-4 68-69 0.002350486614833451 0.0 0.0 0.3537696035925692 1.7094448107879642E-4 70-71 0.002435958855372849 0.0 0.0 0.43723324647929157 1.7094448107879642E-4 72-73 0.002500063035777398 0.0 0.0 0.5387956363002315 1.7094448107879642E-4 74-75 0.0025641672161819465 0.0 0.0 0.6533070705628903 1.7094448107879642E-4 76-77 0.0025641672161819465 0.0 0.0 0.7272619266896045 1.7094448107879642E-4 78-79 0.0025855352763167957 0.0 0.0 0.8322859422523902 1.7094448107879642E-4 80-81 0.0029487922986092385 0.0 0.0 0.951904342887278 1.7094448107879642E-4 82-83 0.003034264539148637 0.0 0.0 1.0349192565111685 1.7094448107879642E-4 84-85 0.0031411048398228844 0.0 0.0 1.1800724890072014 1.7094448107879642E-4 86-87 0.003162472899957734 0.0 0.0 1.4154203033324344 1.9231254121364599E-4 88-89 0.003162472899957734 0.0 0.0 1.7286974329694638 2.1368060134849555E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13895 0.0 30.296228 1 GGTATCA 9505 0.0 25.39364 1 TATCAAC 16685 0.0 25.022854 2 ATCAACG 16645 0.0 24.995243 3 TCAACGC 16715 0.0 24.890566 4 CAACGCA 16805 0.0 24.76361 5 AACGCAG 17355 0.0 24.033573 6 GTACATG 9640 0.0 22.67223 1 TACATGG 9675 0.0 22.092033 2 GAGTACT 9385 0.0 21.381529 12-13 ACGCAGA 19635 0.0 21.049255 7 AGTACTT 9785 0.0 20.96859 12-13 ACATGGG 9775 0.0 20.941006 3 CGCAGAG 19655 0.0 20.906988 8 AGAGTAC 14390 0.0 20.897356 10-11 GTACTTT 9875 0.0 20.12819 14-15 GCAGAGT 20480 0.0 19.600863 9 CCTATAC 565 0.0 19.333765 3 TGTACCG 280 3.4877303E-8 18.66307 5 CATGGGG 5220 0.0 18.196863 4 >>END_MODULE