FastQCFastQC Report
Thu 26 May 2016
SRR937056_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937056_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326184
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84060.3613643632661905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54210.23304261399786086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38730.16649585759338043No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30780.13231971331588557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125300.030.652861
ATCAACG144950.026.0416833
TCAACGC147150.025.6534484
TATCAAC148450.025.55582
CAACGCA148000.025.3765265
AACGCAG152100.024.7517956
GGTATCA87400.024.0392861
GTACATG96250.023.903161
TACATGG96800.023.033732
ACATGGG94850.022.5048313
ACGCAGA170650.021.8907957
CGCAGAG171050.021.7284628
GAGTACT75900.020.88061512-13
AGAGTAC127100.020.20961610-11
GCAGAGT179850.020.1631959
AGTACTT82050.019.46031812-13
GTACTTT81650.019.05991614-15
CATGGGG49100.017.8164754
CATGGGA50350.016.8076114
GAGTACA80150.016.4280851
Produced by FastQC (version 0.11.5)