FastQCFastQC Report
Thu 26 May 2016
SRR937056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2326184
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95330.4098128093048529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67620.29069067623197475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47190.20286443376792204No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41010.17629731783900154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA141250.030.3373091
GGTATCA98750.025.401291
ATCAACG168700.025.28293
TCAACGC169950.025.0969414
TATCAAC170500.025.0727752
CAACGCA171350.024.8918885
AACGCAG177750.024.0794266
GTACATG93350.022.7484661
GAGTACT96800.022.6002512-13
TACATGG93450.021.8561362
ACGCAGA198450.021.5198657
CGCAGAG197950.021.4814628
GTACTTT101550.021.35301214-15
AGAGTAC147600.021.00344310-11
AGTACTT101550.020.86478412-13
ACATGGG94450.020.4671823
GCAGAGT207700.019.9011929
TACTTTT114900.018.35528814-15
ACTTTTT120700.018.23929616-17
ACCGTAT1650.001462104717.2768128
Produced by FastQC (version 0.11.5)