FastQCFastQC Report
Thu 26 May 2016
SRR937055_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937055_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493178
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57121.158202515116246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41590.8433060679916785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28210.5720044284213813No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22950.4653492248234917No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20460.4148603546792436No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20310.4118188564777829No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18110.3672102161896922No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13960.283062099282612No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT10310.2090523097137342No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG9990.20256378021728463No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC9960.2019554805769925No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA9080.1841120244617562No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8760.17762349496530666No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8700.17640689568472234No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8200.16626856834651993No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA7890.1599828053968344No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA7830.1587662061162501No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7790.1579551399291939No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT7640.15491364172773317No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT7540.1528859762600927No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG7410.15025001115216008No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7240.14680297985717125No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG7200.14599191367011505No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT7070.14335594856218242No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC6650.1348397535980924No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA6520.13220378849015973No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA6500.13179825539663165No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT6470.13118995575633952No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT6460.13098718920957544No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC6410.1299733564757552No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT6230.12632355863400233No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA6140.12449865971312589No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC6120.12409312661959779No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG6120.12409312661959779No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG6100.12368759352606969No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT5920.12003779568431681No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC5910.11983502913755278No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC5710.1157796982022718No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA5650.1145630989216875No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA5530.11212990036051891No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG5470.11091330107993462No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT5470.11091330107993462No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5460.11071053453317059No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC5410.10969670179935033No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC5370.10888563561229415No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.10462753813024912No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG5120.10381647194319292No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT5090.10320817230290077No Hit
ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT5000.10138327338202434No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGAC2650.034.061968
GATGACC2350.032.345519
GGTATAT902.4042523E-531.678081
CATCTAC300.003945678531.67486222-23
CGCAGAA1051.8964256E-631.6716469
ATTAGAG804.4831508E-429.6951833
GACAATG3150.028.6582077
GATCTCG853.619789E-927.95550594-95
GGACAAT3250.027.7764176
GATCCTC1056.8858084E-527.1498815
ATCCATC700.00838056627.1471238
GCGCCAC350.0083928827.1416172-73
TACCTGG908.9214626E-426.39842
GGTACTC1457.854451E-726.2136783
GATTGGC1309.993704E-625.5809449
GGTATCA44600.025.463211
ACCCTTG4300.025.413557
TACTCCC1501.0556669E-625.339895
ATCTCGG851.11693225E-725.15995694-95
TATACTC1751.4788384E-724.4348935
Produced by FastQC (version 0.11.5)