##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937055_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 493178 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00828504110078 33.0 31.0 34.0 30.0 34.0 2 32.12407082229945 33.0 31.0 34.0 30.0 34.0 3 32.10043432594317 34.0 31.0 34.0 30.0 34.0 4 35.701543864487064 37.0 35.0 37.0 35.0 37.0 5 35.73091257112036 37.0 35.0 37.0 35.0 37.0 6 35.80925750945906 37.0 35.0 37.0 35.0 37.0 7 35.772729116059516 37.0 35.0 37.0 35.0 37.0 8 35.741697723742746 37.0 35.0 37.0 35.0 37.0 9 37.406640198873426 39.0 37.0 39.0 35.0 39.0 10-11 37.29514090247335 39.0 37.0 39.0 34.0 39.0 12-13 37.23817465499272 39.0 37.0 39.0 34.0 39.0 14-15 38.37060655584799 40.0 38.0 41.0 34.0 41.0 16-17 38.31827859312459 40.0 38.0 41.0 34.0 41.0 18-19 38.363808807367725 40.0 38.0 41.0 34.0 41.0 20-21 38.3385643722956 40.0 38.0 41.0 34.0 41.0 22-23 38.21227933930548 40.0 38.0 41.0 34.0 41.0 24-25 38.05828220236913 40.0 38.0 41.0 33.0 41.0 26-27 38.00790079849466 40.0 37.5 41.0 33.5 41.0 28-29 38.0528105471047 40.0 38.0 41.0 34.0 41.0 30-31 37.88785590598121 40.0 37.0 41.0 33.0 41.0 32-33 37.616335278540404 40.0 36.5 41.0 33.0 41.0 34-35 37.41722562644725 40.0 36.0 41.0 33.0 41.0 36-37 37.26781405496595 40.0 36.0 41.0 33.0 41.0 38-39 37.09300191817154 40.0 36.0 41.0 32.5 41.0 40-41 36.90678213545617 39.0 35.5 41.0 31.5 41.0 42-43 36.676233530287234 39.0 35.0 41.0 31.0 41.0 44-45 36.34261970323088 39.0 35.0 41.0 30.5 41.0 46-47 35.88455689426536 39.0 35.0 41.0 29.5 41.0 48-49 35.46579125589544 39.0 35.0 41.0 27.0 41.0 50-51 34.773067736192615 38.5 34.5 39.5 25.5 40.5 52-53 35.083111979853115 38.5 35.0 40.0 26.5 41.0 54-55 35.432804180235124 39.0 35.0 41.0 26.5 41.0 56-57 35.34329795732981 39.0 35.0 41.0 26.0 41.0 58-59 35.16589446406774 39.0 35.0 41.0 26.0 41.0 60-61 34.92891106253725 39.0 35.0 41.0 25.0 41.0 62-63 34.64799828865034 39.0 35.0 40.5 23.0 41.0 64-65 34.365935828443284 38.0 35.0 40.0 22.5 41.0 66-67 34.00849389064395 38.0 34.0 40.0 21.5 41.0 68-69 33.57916208752215 37.0 34.0 40.0 20.0 41.0 70-71 33.216877070753355 37.0 34.0 39.0 18.5 41.0 72-73 32.802866307905056 36.0 33.0 39.0 15.5 41.0 74-75 32.3474404778802 36.0 33.0 38.5 10.5 40.0 76-77 31.91708571752998 35.0 32.5 37.5 7.5 39.5 78-79 31.459046226717334 35.0 32.0 37.0 4.0 39.0 80-81 30.983860796710317 35.0 32.0 36.5 2.0 39.0 82-83 30.535141470219678 35.0 31.0 36.0 2.0 37.5 84-85 30.105147634322698 35.0 31.0 36.0 2.0 37.0 86-87 29.797202024421203 35.0 31.0 35.0 2.0 37.0 88-89 29.394619184148524 34.0 30.5 35.0 2.0 36.0 90-91 29.044130111237727 34.0 30.0 35.0 2.0 36.0 92-93 28.707032754907964 34.0 29.5 35.0 2.0 36.0 94-95 28.362070084229224 34.0 29.0 35.0 2.0 35.0 96-97 27.99109854859706 34.0 29.0 35.0 2.0 35.0 98-99 27.52856372344265 34.0 29.0 35.0 2.0 35.0 100-101 25.527570167363507 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24738079089723541 1101 2 0.30817879059995335 1101 3 0.33407314033157576 1101 4 0.19248076482727328 1101 5 0.13516974426769224 1101 6 0.1163024285407559 1101 7 0.22476670661439613 1101 8 0.13768555654976922 1101 9 0.16933999558710155 1101 10-11 0.05163317475437168 1101 12-13 0.3234525096595391 1101 14-15 0.5156216864702472 1101 16-17 0.44900847183995296 1101 18-19 0.4067387927253918 1101 20-21 0.22365217219721245 1101 22-23 0.3966174481889979 1101 24-25 0.47173481619625335 1101 26-27 0.38842288147753123 1101 28-29 0.47449246620570307 1101 30-31 0.439048577316548 1101 32-33 0.5169517376511763 1101 34-35 0.34912050317844034 1101 36-37 0.4146789431676794 1101 38-39 0.7359163111360729 1101 40-41 0.617171304215482 1101 42-43 0.4285890906021663 1101 44-45 0.2658938165107543 1101 46-47 0.501333510191941 1101 48-49 0.26463442200307696 1101 50-51 0.06034487519023202 1101 52-53 0.1647123661935339 1101 54-55 0.17773757003710955 1101 56-57 0.34609139602418537 1101 58-59 0.4683715035369289 1101 60-61 0.4961787742735453 1101 62-63 0.5384980154425776 1101 64-65 0.45727666304562575 1101 66-67 0.5286659839632435 1101 68-69 0.4867861921263028 1101 70-71 0.5170661531528467 1101 72-73 0.35636580970462717 1101 74-75 0.555221432210331 1101 76-77 0.7090246610607664 1101 78-79 0.7771594856136019 1101 80-81 0.7504383014946896 1101 82-83 0.6200970852337129 1101 84-85 0.6369683499118004 1101 86-87 0.927067003236921 1101 88-89 0.680563774672418 1101 90-91 0.6854456514550122 1101 92-93 0.1999830720039384 1101 94-95 0.4264479374588106 1101 96-97 0.36914705676161574 1101 98-99 0.2775051966697397 1101 100-101 0.2783558791457139 1102 1 0.31178757055825557 1102 2 0.3658059092440169 1102 3 0.20695449626377638 1102 4 0.3094299173696484 1102 5 0.2470341510473517 1102 6 0.2518956488797386 1102 7 0.34680060491947984 1102 8 0.35971945485485435 1102 9 0.47103491084133964 1102 10-11 0.546548428991656 1102 12-13 0.496333865591744 1102 14-15 0.6681640593516036 1102 16-17 0.7074830481111434 1102 18-19 0.6609760808609764 1102 20-21 0.6168725111802686 1102 22-23 0.7127191431042519 1102 24-25 0.5649551551793053 1102 26-27 0.6443550848673638 1102 28-29 0.47279755095146214 1102 30-31 0.583963831553838 1102 32-33 0.7220364834138948 1102 34-35 0.3991205031784446 1102 36-37 0.5951874177439507 1102 38-39 0.484221395881832 1102 40-41 0.5705611347239525 1102 42-43 0.5099450228055602 1102 44-45 0.7786056809175363 1102 46-47 0.9437063915478703 1102 48-49 1.0036174728505287 1102 50-51 1.1866160616309074 1102 52-53 1.2714920272104777 1102 54-55 1.197229095460841 1102 56-57 1.3520235994140108 1102 58-59 1.1539647238759088 1102 60-61 1.1275347064769363 1102 62-63 1.3901929306968128 1102 64-65 1.3793105613507137 1102 66-67 1.2413778483700213 1102 68-69 1.3927183955161269 1102 70-71 1.2848627633223302 1102 72-73 1.3122980130944626 1102 74-75 1.450984144074738 1102 76-77 1.426821271230267 1102 78-79 1.539871350020384 1102 80-81 1.5885738947150259 1102 82-83 1.4955208140472713 1102 84-85 1.747985299064343 1102 86-87 1.57791446086404 1102 88-89 1.5864959780622492 1102 90-91 1.515954126031282 1102 92-93 1.5093051059022429 1102 94-95 1.5959394628825336 1102 96-97 1.6394860398124642 1102 98-99 1.5986916373477023 1102 100-101 2.1054745232135126 1103 1 0.4919579557121061 1103 2 0.55260701734327 1103 3 0.43426378106315866 1103 4 0.5462503874181976 1103 5 0.5802086171043683 1103 6 0.5310770852385005 1103 7 0.37961761960477247 1103 8 0.341319645495588 1103 9 0.5473180123277359 1103 10-11 0.6562264248809626 1103 12-13 0.3869403414190913 1103 14-15 0.7969031496378634 1103 16-17 0.9472584494992446 1103 18-19 0.8159208248364394 1103 20-21 0.6164465482956274 1103 22-23 0.736590937212263 1103 24-25 0.9170402881809991 1103 26-27 0.8468304356165177 1103 28-29 1.009991855559612 1103 30-31 0.8663340320244828 1103 32-33 1.1483806495096331 1103 34-35 1.153798648005825 1103 36-37 1.0353694200673829 1103 38-39 0.887438458227308 1103 40-41 1.2372570925447022 1103 42-43 1.3539338822378113 1103 44-45 1.7789839836192698 1103 46-47 1.7274825674139507 1103 48-49 1.7859995883668986 1103 50-51 1.58925971240091 1103 52-53 1.734407936775284 1103 54-55 1.8537466254718638 1103 56-57 1.6319646199376763 1103 58-59 1.791908186983946 1103 60-61 1.4767855479770446 1103 62-63 1.6510728568235322 1103 64-65 1.6430188214561596 1103 66-67 1.5874026912403494 1103 68-69 1.7723585611352632 1103 70-71 1.7781352306304328 1103 72-73 1.4750575376841368 1103 74-75 1.8111878913566173 1103 76-77 1.6805700424911691 1103 78-79 1.7468356942460517 1103 80-81 1.755989223421352 1103 82-83 1.6646653137642922 1103 84-85 1.7842740601974647 1103 86-87 1.6405310049050286 1103 88-89 1.620133641521786 1103 90-91 1.4253655227725162 1103 92-93 1.4713854728856504 1103 94-95 1.5837890950054998 1103 96-97 1.5507368217373418 1103 98-99 1.6234242935092702 1103 100-101 2.0380025980399985 1104 1 0.5064670104992963 1104 2 0.5303895496272162 1104 3 0.5283693820412267 1104 4 0.279555193714522 1104 5 0.4504829351298909 1104 6 0.21121768277804165 1104 7 0.29322654449206453 1104 8 0.3703163597922128 1104 9 0.3604822210845242 1104 10-11 0.4805941180115525 1104 12-13 0.5331724801827491 1104 14-15 0.6246268449079722 1104 16-17 0.6018087666078173 1104 18-19 0.812980502379034 1104 20-21 0.8027826069798252 1104 22-23 0.6884744857718985 1104 24-25 0.7633855162699419 1104 26-27 0.8056078483161428 1104 28-29 0.8317584942086498 1104 30-31 0.8445459980976864 1104 32-33 0.8683592542318692 1104 34-35 1.0087004589632542 1104 36-37 0.9993657523054793 1104 38-39 0.8843835182104982 1104 40-41 1.0031698303761303 1104 42-43 0.8038949122676087 1104 44-45 0.99738239425578 1104 46-47 0.8129842102656326 1104 48-49 0.6260714153707951 1104 50-51 0.7368076607465994 1104 52-53 0.874845011735168 1104 54-55 0.7754383806487581 1104 56-57 0.9317583083307355 1104 58-59 0.9420266251286762 1104 60-61 0.9510424441335346 1104 62-63 0.8127279343814067 1104 64-65 0.8058323004811498 1104 66-67 0.7465805012808531 1104 68-69 1.062799456680466 1104 70-71 1.0381199482891716 1104 72-73 0.9233665466243082 1104 74-75 0.8018610784888835 1104 76-77 0.9208374834631314 1104 78-79 0.7160983212805156 1104 80-81 0.7990823692912983 1104 82-83 0.8801707772012897 1104 84-85 0.9064303985484976 1104 86-87 0.981304291372517 1104 88-89 1.1271665749671875 1104 90-91 1.1449666536657688 1104 92-93 1.0075954522544883 1104 94-95 1.1548046065818731 1104 96-97 1.4284322446761344 1104 98-99 1.4077262725724644 1104 100-101 1.5545533736188162 1105 1 0.3550866351132207 1105 2 0.34966253608480713 1105 3 0.3916971716983113 1105 4 0.47247829500540917 1105 5 0.4969801236940796 1105 6 0.4279754241259468 1105 7 0.44200297594846916 1105 8 0.414342652656714 1105 9 0.4327495846704892 1105 10-11 0.5757077633746661 1105 12-13 0.6271742224809955 1105 14-15 0.5744312323317544 1105 16-17 0.6654343308748665 1105 18-19 0.7317719809566867 1105 20-21 0.6016120993374656 1105 22-23 0.5930994956713249 1105 24-25 0.5351428616409777 1105 26-27 0.6076257464708945 1105 28-29 0.5499686787338689 1105 30-31 0.46365109036029395 1105 32-33 0.38949966763173194 1105 34-35 0.29910197951446094 1105 36-37 0.3067847750345578 1105 38-39 0.39567519477658664 1105 40-41 0.6240219726110254 1105 42-43 0.5325763092740274 1105 44-45 0.4979937825507079 1105 46-47 0.2975284408825658 1105 48-49 0.8033565979161423 1105 50-51 0.8531329953470603 1105 52-53 0.6305747971157061 1105 54-55 0.660226533020662 1105 56-57 1.0816830727244948 1105 58-59 0.9596792742139684 1105 60-61 0.9027253458520761 1105 62-63 0.6953857311660769 1105 64-65 0.9222067362141004 1105 66-67 1.1515967363327277 1105 68-69 1.2228533095354592 1105 70-71 0.9843209461508948 1105 72-73 1.1388238999148754 1105 74-75 1.1318491991826392 1105 76-77 0.9051979808100867 1105 78-79 0.9992582167426178 1105 80-81 0.8556943911492212 1105 82-83 0.9438660333983293 1105 84-85 0.8137828971625787 1105 86-87 1.0242915409171403 1105 88-89 1.2549609837737137 1105 90-91 1.2109110585125293 1105 92-93 1.1063536687746485 1105 94-95 1.1194058578687986 1105 96-97 1.1987339790548468 1105 98-99 1.6381563034586613 1105 100-101 1.6396012868206853 1106 1 0.3724799614706207 1106 2 0.378752176824257 1106 3 0.5761214634473362 1106 4 0.2936347496812246 1106 5 0.5275967186636521 1106 6 0.24738789554400142 1106 7 0.5118204390341532 1106 8 0.29747639679499116 1106 9 0.550731989817173 1106 10-11 0.6233785768459654 1106 12-13 0.5547543488228968 1106 14-15 0.7026934499033501 1106 16-17 0.6562028461637865 1106 18-19 0.7188916957185398 1106 20-21 0.7832266402823151 1106 22-23 0.7711756883620993 1106 24-25 0.8389544339387669 1106 26-27 0.9470417058554617 1106 28-29 0.7190110381494463 1106 30-31 0.6843424828340403 1106 32-33 0.7711529637600947 1106 34-35 0.6918179427745415 1106 36-37 0.715868990048314 1106 38-39 0.7241059973964354 1106 40-41 0.34077390068140545 1106 42-43 0.2581779834979514 1106 44-45 0.1325905348663312 1106 46-47 -0.19846814866128426 1106 48-49 -0.4159822386532497 1106 50-51 -0.22852998957717574 1106 52-53 -0.4180319540372679 1106 54-55 -0.3798715175936138 1106 56-57 -0.3963175473584428 1106 58-59 -0.2490464553523637 1106 60-61 -0.27217679730957656 1106 62-63 -0.16265596941136806 1106 64-65 -0.3940936939684363 1106 66-67 -0.4311068252686354 1106 68-69 -0.5224456867052965 1106 70-71 -0.5609179795554198 1106 72-73 -0.5134142119325844 1106 74-75 -0.7393692637867844 1106 76-77 -0.6317434601076357 1106 78-79 -0.5853540376464039 1106 80-81 -0.3798538009214667 1106 82-83 -0.15471358912618527 1106 84-85 -0.24489245066519416 1106 86-87 -0.09954677245727339 1106 88-89 -0.0218343501021927 1106 90-91 0.22011929011350517 1106 92-93 0.16916807020083624 1106 94-95 0.3327046990888114 1106 96-97 0.42278932145689296 1106 98-99 0.5520093438028049 1106 100-101 0.8517781654782048 1107 1 0.015742372818138506 1107 2 0.22343302788808472 1107 3 0.15086535627633424 1107 4 0.080553583408566 1107 5 0.03890483529092137 1107 6 0.2686250901854521 1107 7 0.2004729213036569 1107 8 0.0831343952189485 1107 9 0.2718509811489298 1107 10-11 0.3192414610067189 1107 12-13 0.3008536396030408 1107 14-15 0.07968320561651154 1107 16-17 0.19258976499752123 1107 18-19 0.19432510785406976 1107 20-21 0.1708743944194353 1107 22-23 0.2930236001036306 1107 24-25 0.40012464670472525 1107 26-27 0.3648510045158204 1107 28-29 0.36077620757419027 1107 30-31 0.23479559552119866 1107 32-33 0.4157747131690712 1107 34-35 0.29670367957517385 1107 36-37 0.16742533362592837 1107 38-39 0.2530067066162971 1107 40-41 0.3020426162054406 1107 42-43 0.11871777861221489 1107 44-45 0.22827611406254533 1107 46-47 0.3352788962559643 1107 48-49 0.14288300957370126 1107 50-51 0.1589010584921695 1107 52-53 0.09296095376415536 1107 54-55 0.27698427436856576 1107 56-57 0.1557116094579527 1107 58-59 0.0921473980755323 1107 60-61 0.14584920741855 1107 62-63 0.26801778945387866 1107 64-65 0.4756068576470156 1107 66-67 0.6576835608621749 1107 68-69 0.605351791624102 1107 70-71 0.48315215440833725 1107 72-73 0.5343005240800238 1107 74-75 0.7571831396805067 1107 76-77 0.6210146171185187 1107 78-79 0.7789485628264075 1107 80-81 0.7635268137357443 1107 82-83 0.8765314857359137 1107 84-85 1.0038861151346516 1107 86-87 0.933156143224366 1107 88-89 1.0978268004100222 1107 90-91 1.2223257518944308 1107 92-93 1.1109529401771958 1107 94-95 1.012590435889443 1107 96-97 0.9326592978162331 1107 98-99 1.0495861759541079 1107 100-101 1.087081553973082 1108 1 0.142769399845168 1108 2 0.3810906855457432 1108 3 0.23199648740746426 1108 4 0.11408711694210183 1108 5 0.16528121166729903 1108 6 0.1661726377329984 1108 7 0.04731976815050842 1108 8 0.01476602685058026 1108 9 -0.05147234217439234 1108 10-11 0.13786007962534086 1108 12-13 0.08058336933276422 1108 14-15 0.4704239463572506 1108 16-17 0.4628350102427632 1108 18-19 0.40080658933555924 1108 20-21 0.4245530730981173 1108 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-2.280417714477899 2316 98-99 -2.3454787741734755 2316 100-101 -2.347697692914725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1212.0 3 260.0 4 275.0 5 269.0 6 261.0 7 315.0 8 439.0 9 551.0 10 710.0 11 1056.0 12 1498.0 13 2116.0 14 2591.0 15 3517.0 16 4856.0 17 7163.0 18 5682.0 19 3126.0 20 2535.0 21 2486.0 22 2708.0 23 2993.0 24 3384.0 25 3727.0 26 4332.0 27 5027.0 28 6037.0 29 7205.0 30 8637.0 31 10887.0 32 13992.0 33 18508.0 34 25707.0 35 38938.0 36 58574.0 37 98821.0 38 117825.0 39 24956.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.45616996413095 23.572541509097107 16.988703682664372 20.982584844107574 2 13.478995894361494 23.874399527537 41.44584230532718 21.20076227277432 3 15.295089185169555 31.392449239222405 32.320518777480416 20.99194279812763 4 9.97342396391019 20.21342039066164 43.200981019147335 26.612174626280837 5 9.816569812875633 39.93468204689983 37.454913765281624 12.793834374942914 6 23.539798940102173 43.20210112261487 19.615416621844613 13.642683315438338 7 22.272741112114723 35.18252768108147 26.988724589731362 15.556006617072454 8 21.92676600598309 35.35583960123559 23.987765920742326 18.729628472039 9 24.459393351345096 17.609374777982346 25.0044149796708 32.926816891001764 10-11 20.3906166750392 29.221557493923573 31.084420493928643 19.303405337108583 12-13 22.78324823236218 28.24293553702907 30.441625333842044 18.532190896766704 14-15 20.989517266573984 29.520615365880886 29.41477324619709 20.07509412134804 16-17 16.969390043772 32.0611407514008 31.135463848262468 19.834005356564735 18-19 16.769416840340842 30.600814560626322 34.556121279026584 18.073647320006252 20-21 17.82239689325431 29.7508638303139 34.37791029150088 18.048828984930903 22-23 18.21963394342762 28.773791648066233 34.69106367436387 18.315510734142283 24-25 18.124430026242884 29.79921707542471 34.11879394313805 17.957558955194354 26-27 17.269098710269663 29.673433989440746 34.89311416877565 18.16435313151395 28-29 17.134813792823593 28.727136234060037 35.69192375194019 18.446126221176186 30-31 17.75662637893191 29.500115629450214 34.38180844460132 18.361449547016555 32-33 17.187281288100248 30.020509470669023 34.65157229131969 18.140636949911045 34-35 17.92490052428242 29.168116244843645 34.66937953320709 18.237603697666845 36-37 17.87467935486554 28.810542213584906 34.18003596702718 19.134742464522372 38-39 17.1897822064981 30.1582938427083 34.37753578499789 18.27438816579571 40-41 17.010959760110694 29.73306633880713 35.10704127053687 18.148932630545307 42-43 17.274815029710886 29.75125629669904 33.10161751369906 19.872311159891016 44-45 17.15782427457225 29.716522815095793 33.58489609217319 19.540756818158766 46-47 18.30906356451729 29.640217875219687 33.198589551035475 18.85212900922755 48-49 17.947324062795705 30.11508218634856 32.57981056622221 19.357783184633533 50-51 17.655976522650896 29.974283622439767 32.7153695918032 19.654370263106124 52-53 17.71148143490554 30.86839938177201 31.955064885542633 19.465054297779815 54-55 18.163322021108492 30.190962255845605 32.110625850698916 19.535089872346987 56-57 18.360611175124557 30.458239341761466 31.163176130738897 20.01797335237508 58-59 17.940132612622847 29.8908892470577 32.3210049103389 19.847973229980557 60-61 18.68346767854118 30.937332956010437 31.500882955858295 18.878316409590088 62-63 18.013833621237925 30.784828256373377 32.595432003968114 18.605906118420585 64-65 18.19280835289882 30.092240911557244 32.2683962048874 19.446554530656538 66-67 17.969804836136 30.59129406251046 31.20745150828729 20.23144959306625 68-69 17.76009964489335 30.21875421568697 32.38215601769346 19.63899012172622 70-71 19.079677893347043 29.448867444857846 31.98848431195386 19.48297034984125 72-73 18.113466590820828 29.68573662228468 31.910818273168722 20.289978513725764 74-75 17.756468177065745 30.946752702827407 31.272379897717972 20.024399222388872 76-77 17.508471464779905 30.211358101823915 32.393400523552245 19.886769909843938 78-79 17.674208649695935 29.487987096970524 32.47414575757115 20.36365849576239 80-81 18.056111960226897 31.006305318597597 30.85681135667292 20.080771364502585 82-83 18.62979208595728 29.5452793287022 31.63263649938324 20.19229208595728 84-85 17.740978308173467 29.333318454841578 31.51396430987976 21.411738927105198 86-87 17.653628904633216 30.279725529342066 31.952052848992444 20.114592717032277 88-89 18.125059613562062 29.145603208072135 31.826437777646994 20.902899400718812 90-91 17.734597300628433 28.71285320835679 32.34569404078983 21.20685545022495 92-93 18.36638002751411 29.916118481123615 31.74024730235899 19.977254189003283 94-95 17.42428777393018 28.530844444083698 32.69913870335742 21.345729078628704 96-97 17.126349189598365 29.174458827456952 32.455639224392925 21.243552758551758 98-99 17.19484157797774 29.729949197358874 31.725310474682423 21.349898749980966 100-101 16.94122017465866 29.167427905598252 32.37325879281715 21.518093126925937 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1680.0 1 1129.0 2 593.5 3 592.0 4 493.5 5 483.5 6 735.0 7 1371.5 8 2216.5 9 2490.5 10 1949.0 11 1440.5 12 1356.5 13 1275.5 14 1229.0 15 1259.0 16 1290.5 17 1306.0 18 1299.5 19 1364.5 20 1502.0 21 1626.0 22 1693.0 23 1887.0 24 2099.0 25 2359.5 26 2807.0 27 3249.5 28 3362.0 29 4178.0 30 6534.0 31 8669.0 32 12464.0 33 17950.5 34 20762.0 35 24033.5 36 31665.0 37 36405.5 38 34968.0 39 32423.5 40 28883.0 41 24802.0 42 20015.5 43 18281.5 44 18438.5 45 17315.5 46 14771.5 47 12911.5 48 15471.5 49 13737.5 50 7897.0 51 4392.0 52 2983.5 53 2494.0 54 2096.5 55 1818.0 56 1651.0 57 1551.0 58 1483.0 59 1403.0 60 1355.5 61 1288.5 62 1005.5 63 620.0 64 331.5 65 224.0 66 146.0 67 102.0 68 73.5 69 51.5 70 45.5 71 37.5 72 32.5 73 23.5 74 16.0 75 23.5 76 21.0 77 11.0 78 7.5 79 5.0 80 2.5 81 0.5 82 1.0 83 1.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05555803381334934 2 0.08942004712294548 3 0.06630466079184391 4 0.12754015791458662 5 0.10361370539642886 6 0.09915284136761982 7 0.10401923848995698 8 0.0924615453244062 9 0.10888563561229415 10-11 0.10047082392158613 12-13 0.08718961510854092 14-15 0.09388091115175454 16-17 0.1037150886698109 18-19 0.08049831906532733 20-21 0.06387146223067533 22-23 0.07259042374152944 24-25 0.05728154946084375 26-27 0.04977918723057395 28-29 0.06346592913714724 30-31 0.045419706475146905 32-33 0.047650138489551436 34-35 0.044304490467944636 36-37 0.04592662284205703 38-39 0.04744737194278739 40-41 0.057585699280989826 42-43 0.05292206870541671 44-45 0.039133943525461394 46-47 0.029096999460640986 48-49 0.037004894784438885 50-51 0.02169602050375321 52-53 0.03163158129519159 54-55 0.040756075899573786 56-57 0.045825239568675 58-59 0.04835982140322561 60-61 0.04694045557587727 62-63 0.0503874868708661 64-65 0.035585528957090544 66-67 0.05768708255437185 68-69 0.04612938938882107 70-71 0.022608469964191427 72-73 0.014700574640393529 74-75 0.026258267805944303 76-77 0.040451926079427714 78-79 0.054848350899675165 80-81 0.036802128237674835 82-83 0.05718016618746173 84-85 0.060120281115540436 86-87 0.0612354971227427 88-89 0.08536471618766449 90-91 0.0578898491011359 92-93 0.06894062589977655 94-95 0.07593607176313623 96-97 0.08688546528839486 98-99 0.11871981313035049 100-101 0.11354926618786726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 493178.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.766257330248237 #Duplication Level Percentage of deduplicated Percentage of total 1 74.18285845283302 15.40500328124911 2 10.257026841818062 4.260001176809146 3 3.630810388746096 2.2619502855012024 4 1.9123562397638676 1.5884992712816948 5 1.20110378965004 1.247121518810454 6 0.8047102812533328 1.0026492466081884 7 0.6709227369457237 0.9752787942890541 8 0.5750764259055886 0.955374803593191 9 0.45826400685292396 0.8564785462348614 >10 4.671497331537801 21.125848690477394 >50 0.8955616811417082 13.163148412199197 >100 0.6918887904472729 26.86867103483348 >500 0.03893746439746558 5.458780805254338 >1k 0.007987172184095503 3.646927508238969 >5k 9.98396523011938E-4 1.1842666246197264 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5712 1.158202515116246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4159 0.8433060679916785 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2821 0.5720044284213813 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2295 0.4653492248234917 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2046 0.4148603546792436 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2031 0.4118188564777829 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1811 0.3672102161896922 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1396 0.283062099282612 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1031 0.2090523097137342 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 999 0.20256378021728463 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 996 0.2019554805769925 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 908 0.1841120244617562 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 876 0.17762349496530666 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 870 0.17640689568472234 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 820 0.16626856834651993 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 789 0.1599828053968344 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 783 0.1587662061162501 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 779 0.1579551399291939 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 764 0.15491364172773317 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 754 0.1528859762600927 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 741 0.15025001115216008 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 724 0.14680297985717125 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 720 0.14599191367011505 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 707 0.14335594856218242 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 665 0.1348397535980924 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 652 0.13220378849015973 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 650 0.13179825539663165 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 647 0.13118995575633952 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 646 0.13098718920957544 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 641 0.1299733564757552 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 623 0.12632355863400233 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 614 0.12449865971312589 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 612 0.12409312661959779 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 612 0.12409312661959779 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 610 0.12368759352606969 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 592 0.12003779568431681 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 591 0.11983502913755278 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 571 0.1157796982022718 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 565 0.1145630989216875 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 553 0.11212990036051891 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 547 0.11091330107993462 No Hit GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT 547 0.11091330107993462 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 546 0.11071053453317059 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 541 0.10969670179935033 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 537 0.10888563561229415 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 516 0.10462753813024912 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 512 0.10381647194319292 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 509 0.10320817230290077 No Hit ACCTAAAACCTGGTGAACTACGACTGCTAGAAGTTGATAACCGAGTCGTT 500 0.10138327338202434 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.0276654676404867E-4 0.0 20-21 0.0 0.0 0.0 2.0276654676404867E-4 0.0 22-23 0.0 0.0 0.0 9.12449460438219E-4 0.0 24-25 0.0 0.0 0.0 0.0014193658273483408 0.0 26-27 0.0 0.0 0.0 0.0034470312949888273 0.0 28-29 0.0 0.0 0.0 0.007907895323797898 0.0 30-31 0.0 0.0 0.0 0.019668355036112722 0.0 32-33 0.0 0.0 0.0 0.02899561618725896 0.0 34-35 0.0 0.0 0.0 0.0379173442448771 0.0 36-37 0.0 0.0 0.0 0.051299936331304315 0.0 38-39 0.0 0.0 0.0 0.07005584190697882 0.0 40-41 0.0 0.0 0.0 0.09286707841793429 0.0 42-43 0.0 0.0 0.0 0.12845260737502484 0.0 44-45 0.0 0.0 0.0 0.15613024100831746 0.0 46-47 0.0 0.0 0.0 0.21827818759149842 0.0 48-49 0.0 0.0 0.0 0.29472117572154477 0.0 50-51 0.0 0.0 0.0 0.328988722124669 0.0 52-53 0.0 0.0 0.0 0.378970675902007 0.0 54-55 0.0 0.0 0.0 0.4651464582767277 0.0 56-57 0.0 0.0 0.0 0.5950184314791008 0.0 58-59 0.0 0.0 0.0 0.7598676339982724 0.0 60-61 0.0 0.0 0.0 0.9419519929923881 0.0 62-63 0.0 0.0 0.0 1.1335663796844142 0.0 64-65 0.0 0.0 0.0 1.3500196683550363 0.0 66-67 0.0 0.0 0.0 1.616556294076378 0.0 68-69 0.0 0.0 0.0 1.9293236924599233 0.0 70-71 0.0 0.0 0.0 2.1065416543317017 0.0 72-73 0.0 0.0 0.0 2.3346540194412566 0.0 74-75 0.0 0.0 0.0 2.5838541054142725 0.0 76-77 0.0 0.0 0.0 2.853330846063693 0.0 78-79 0.0 0.0 0.0 3.1504852203464067 0.0 80-81 0.0 0.0 0.0 3.6098528320403585 0.0 82-83 0.0 0.0 0.0 4.076824189237962 0.0 84-85 0.0 0.0 0.0 4.8080003568691225 0.0 86-87 0.0 0.0 0.0 5.765666757235724 0.0 88-89 0.0 0.0 0.0 6.693222325407865 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATGAC 265 0.0 34.06196 8 GATGACC 235 0.0 32.34551 9 GGTATAT 90 2.4042523E-5 31.67808 1 CATCTAC 30 0.0039456785 31.674862 22-23 CGCAGAA 105 1.8964256E-6 31.671646 9 ATTAGAG 80 4.4831508E-4 29.695183 3 GACAATG 315 0.0 28.658207 7 GATCTCG 85 3.619789E-9 27.955505 94-95 GGACAAT 325 0.0 27.776417 6 GATCCTC 105 6.8858084E-5 27.149881 5 ATCCATC 70 0.008380566 27.147123 8 GCGCCAC 35 0.00839288 27.14161 72-73 TACCTGG 90 8.9214626E-4 26.3984 2 GGTACTC 145 7.854451E-7 26.213678 3 GATTGGC 130 9.993704E-6 25.580944 9 GGTATCA 4460 0.0 25.46321 1 ACCCTTG 430 0.0 25.41355 7 TACTCCC 150 1.0556669E-6 25.33989 5 ATCTCGG 85 1.11693225E-7 25.159956 94-95 TATACTC 175 1.4788384E-7 24.434893 5 >>END_MODULE