FastQCFastQC Report
Thu 26 May 2016
SRR937054_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937054_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408985
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51241.252857684267149No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38370.9381762167316651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.6332750589874935No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19780.48363631918041No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18080.4420700025673313No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16630.406616379573823No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15180.37116275658031467No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11630.284362507182415No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC9090.22225754000757972No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT8440.2063645365966967No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG8130.19878479650842942No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA7980.19511718033668718No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7530.18411433182146045No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7510.18362531633189483No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7490.18313630084232918No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7060.1726224678166681No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA6800.16626526645231487No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT6630.162108634791007No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT6410.15672946440578506No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG6300.15403987921317408No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA6130.14988324755186622No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6080.14866070882795213No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT5660.13839138354707384No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC5620.13741335256794257No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG5550.1357017983544629No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA5440.1330122131618519No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA5260.12861107375576122No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT5220.12763304277662996No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC5100.12469894983923617No Hit
CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC4950.12103133366749393No Hit
GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG4920.12029781043314547No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT4890.11956428719879701No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC4890.11956428719879701No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT4840.11834174847488294No Hit
TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC4830.11809724073010013No Hit
ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC4800.11736371749575167No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4800.11736371749575167No Hit
TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA4760.11638568651662042No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT4750.1161411787718376No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC4720.11540765553748915No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA4630.1132070858344438No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT4480.10953946966270156No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4420.10807242319400467No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG4350.10636086898052495No Hit
CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC4320.10562734574617651No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4280.10464931476704524No Hit
GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG4220.10318226829834835No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT4210.10293776055356554No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA4190.1024487450639999No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA4180.10220423731921709No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA4150.10147071408486863No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGCT200.002071308271.265694
GTATGGT602.3423127E-855.4288671
TGGACTG558.1514736E-751.8422745
GGTACTC802.2495988E-741.5818258
CTTTGCG704.296864E-640.7232482
GTGGTAC853.619807E-739.1358386
TGGTACT853.619807E-739.1358387
CATATAG1101.6116246E-938.8721923
GACTGGT500.001610914638.017677
ACCTGTG550.002571819834.5615167
CATGGAT550.002574908334.5530624
TATCTAA352.0531114E-433.9277470-71
GGATATG1151.0294025E-733.058849
ACATATA1308.241841E-932.8918572
GTGGCGA300.003948399331.66976420-21
CCGCCGA300.0039531331.66202284-85
CTGTGCC804.4764273E-429.7013039
GCTTTGC804.482848E-429.6940381
GAGAGCG650.005826473229.2443629
GGACTGG1004.9297607E-528.513256
Produced by FastQC (version 0.11.5)