##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937054_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 408985 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.070701859481396 33.0 31.0 34.0 30.0 34.0 2 32.19689964179615 33.0 31.0 34.0 30.0 34.0 3 32.184444417276914 34.0 31.0 34.0 30.0 34.0 4 35.780192427595146 37.0 35.0 37.0 35.0 37.0 5 35.79354255046029 37.0 35.0 37.0 35.0 37.0 6 35.880379476019904 37.0 35.0 37.0 35.0 37.0 7 35.836321625487486 37.0 35.0 37.0 35.0 37.0 8 35.79635194444784 37.0 35.0 37.0 35.0 37.0 9 37.4811105541768 39.0 37.0 39.0 35.0 39.0 10-11 37.33690966661369 39.0 37.0 39.0 34.5 39.0 12-13 37.31905082093475 39.0 37.0 39.0 34.5 39.0 14-15 38.48702152890693 40.0 38.0 41.0 34.0 41.0 16-17 38.442860985121705 40.0 38.0 41.0 34.0 41.0 18-19 38.50574715454112 40.0 38.0 41.0 34.0 41.0 20-21 38.455130383754906 40.0 38.0 41.0 34.0 41.0 22-23 38.35586268445053 40.0 38.0 41.0 34.0 41.0 24-25 38.21895179529811 40.0 38.0 41.0 34.0 41.0 26-27 38.25968923065638 40.0 38.0 41.0 34.0 41.0 28-29 38.20741347482182 40.0 38.0 41.0 34.0 41.0 30-31 38.01277675220363 40.0 37.5 41.0 34.0 41.0 32-33 37.71819137621184 40.0 37.0 41.0 33.0 41.0 34-35 37.52419770896243 40.0 36.0 41.0 33.0 41.0 36-37 37.35556683007934 40.0 36.0 41.0 33.0 41.0 38-39 37.180156973972146 40.0 36.0 41.0 33.0 41.0 40-41 37.028560949668076 40.0 36.0 41.0 32.5 41.0 42-43 36.769375404965956 40.0 35.0 41.0 31.0 41.0 44-45 36.40361993716151 39.5 35.0 41.0 30.5 41.0 46-47 35.91463990121887 39.0 35.0 41.0 29.5 41.0 48-49 35.49183344132425 39.0 35.0 41.0 27.0 41.0 50-51 34.77340367006125 38.5 34.5 40.0 25.0 40.5 52-53 35.00577527293177 38.5 35.0 40.0 26.0 40.5 54-55 35.41897380099515 39.5 35.0 41.0 26.5 41.0 56-57 35.348042104233656 39.5 35.0 41.0 26.0 41.0 58-59 35.16802327713731 39.0 35.0 41.0 25.0 41.0 60-61 34.93788525251537 39.0 35.0 41.0 23.5 41.0 62-63 34.64538430504786 39.0 35.0 41.0 22.5 41.0 64-65 34.35623800383877 38.5 35.0 40.0 22.0 41.0 66-67 33.999892416592296 38.0 34.5 40.0 20.0 41.0 68-69 33.61676467352103 37.0 34.0 40.0 18.5 41.0 70-71 33.188253847940636 37.0 34.0 39.0 13.5 41.0 72-73 32.77438170104038 36.0 34.0 39.0 8.0 41.0 74-75 32.319137621184154 36.0 33.0 39.0 4.5 40.0 76-77 31.885253737912148 35.5 33.0 37.5 2.0 40.0 78-79 31.40952968935291 35.0 32.0 37.0 2.0 39.0 80-81 30.93593652578945 35.0 32.0 37.0 2.0 39.0 82-83 30.505585779429566 35.0 31.5 36.0 2.0 38.5 84-85 30.075269264153942 35.0 31.0 36.0 2.0 37.0 86-87 29.763432644229006 35.0 31.0 35.0 2.0 37.0 88-89 29.384374732569654 34.5 30.5 35.0 2.0 36.0 90-91 29.044364707752116 34.0 30.0 35.0 2.0 36.0 92-93 28.717338044182547 34.0 30.0 35.0 2.0 36.0 94-95 28.359790701370464 34.0 29.0 35.0 2.0 35.5 96-97 27.979899018301403 34.0 29.0 35.0 2.0 35.0 98-99 27.54259813929606 34.0 29.0 35.0 2.0 35.0 100-101 25.54967786104625 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24191727465768764 1101 2 0.17946514572276584 1101 3 0.2889187774215358 1101 4 0.24554832058434783 1101 5 0.26998746790973627 1101 6 0.24535892694827766 1101 7 0.25625511178071747 1101 8 0.26650646153438373 1101 9 0.103178291912144 1101 10-11 0.39355899742801626 1101 12-13 0.33543467350117595 1101 14-15 0.6006582881073328 1101 16-17 0.6753775713680241 1101 18-19 0.6486216510440244 1101 20-21 0.742544486967077 1101 22-23 0.6335269983499145 1101 24-25 0.6286944952146243 1101 26-27 0.6649170688453694 1101 28-29 0.7261647723746378 1101 30-31 0.5834535481346919 1101 32-33 0.8203601770141802 1101 34-35 0.6425144772774019 1101 36-37 0.5366372792537462 1101 38-39 0.4243754552896206 1101 40-41 0.46872817631121677 1101 42-43 0.526304734202526 1101 44-45 0.44255646613959243 1101 46-47 0.23898086253952044 1101 48-49 0.22082236582001968 1101 50-51 0.3340394225352483 1101 52-53 0.3220950533233662 1101 54-55 0.5274504061475298 1101 56-57 0.6194463456605419 1101 58-59 0.47579464252755344 1101 60-61 0.6339351617177513 1101 62-63 0.666758587152458 1101 64-65 0.7084789906365216 1101 66-67 0.5356813962875933 1101 68-69 0.7900290930813725 1101 70-71 0.8205708063708101 1101 72-73 0.8795444595781774 1101 74-75 0.8263138853887568 1101 76-77 0.8207227672272843 1101 78-79 0.8187779688213972 1101 80-81 0.7518814482354124 1101 82-83 0.881484383573536 1101 84-85 0.8778445408425455 1101 86-87 0.9986543359455631 1101 88-89 1.114596774496885 1101 90-91 1.5599456952116526 1101 92-93 1.5399669091499995 1101 94-95 1.3844957197616168 1101 96-97 1.2188425909901568 1101 98-99 1.2455132064894343 1101 100-101 1.3865990284157554 1102 1 0.4332206218004515 1102 2 0.3194894766230121 1102 3 0.3412367888636325 1102 4 0.4746073065776457 1102 5 0.49886232817145526 1102 6 0.46439621240892137 1102 7 0.495250311359861 1102 8 0.6015035023708393 1102 9 0.556285413632402 1102 10-11 0.6858520203779719 1102 12-13 0.7298599381819173 1102 14-15 0.8503344240973334 1102 16-17 0.9698570873803902 1102 18-19 0.9317379790508653 1102 20-21 0.8554053405213651 1102 22-23 0.8508594768137812 1102 24-25 0.9913186156854579 1102 26-27 0.9878472983449385 1102 28-29 0.781492910731977 1102 30-31 0.9356482610958281 1102 32-33 1.0291932801903485 1102 34-35 1.0686553992212424 1102 36-37 0.8630067143823013 1102 38-39 0.9133344873143514 1102 40-41 0.9808163593847965 1102 42-43 1.0368344244767442 1102 44-45 1.3303219688686028 1102 46-47 1.3156379941236622 1102 48-49 1.437640278623327 1102 50-51 1.4873964291769255 1102 52-53 1.3019217778581194 1102 54-55 1.4687175857358739 1102 56-57 1.6390228564779292 1102 58-59 1.6560751054722473 1102 60-61 1.6587822716013605 1102 62-63 1.7409809847325235 1102 64-65 2.1624081022298753 1102 66-67 1.986225553583573 1102 68-69 1.8465392528761981 1102 70-71 1.8364631585770326 1102 72-73 1.7274500293815578 1102 74-75 1.6868780992376387 1102 76-77 1.7004393451190403 1102 78-79 1.687361515872098 1102 80-81 1.9431601356819819 1102 82-83 1.6662479792860374 1102 84-85 1.6414155936471708 1102 86-87 1.69076816510319 1102 88-89 2.035342154913799 1102 90-91 2.117124954763174 1102 92-93 1.9492899182905283 1102 94-95 1.9408612093387845 1102 96-97 1.839839500308237 1102 98-99 1.8246116479966332 1102 100-101 2.236377091150022 1103 1 0.3365640374182348 1103 2 0.4593210049021934 1103 3 0.4305430626464428 1103 4 0.4244463152614202 1103 5 0.5119042509606757 1103 6 0.43421765840364657 1103 7 0.3820555281289799 1103 8 0.43245286328410515 1103 9 0.43346367492618754 1103 10-11 0.5756981842313564 1103 12-13 0.5375842690109351 1103 14-15 0.6052796220028256 1103 16-17 0.7754592599500683 1103 18-19 0.708517827491363 1103 20-21 0.745723095099045 1103 22-23 0.8366515866131152 1103 24-25 0.7225671927824209 1103 26-27 0.6207854711748126 1103 28-29 0.5980685116036071 1103 30-31 0.557230854519581 1103 32-33 0.5953509541097546 1103 34-35 0.7004178475901952 1103 36-37 0.7223799865714611 1103 38-39 0.7481850418399887 1103 40-41 0.6855290142779609 1103 42-43 0.6815558607144183 1103 44-45 0.5759394274824459 1103 46-47 0.5774976877523059 1103 48-49 0.616774828335501 1103 50-51 0.4550241652162086 1103 52-53 0.6287796826943648 1103 54-55 0.6080124762942987 1103 56-57 0.6638480426749567 1103 58-59 0.7203366594450031 1103 60-61 0.5601189873785088 1103 62-63 0.4559954576892338 1103 64-65 0.46610765015324773 1103 66-67 0.6725591796283936 1103 68-69 0.5861040884699165 1103 70-71 0.2511849200496954 1103 72-73 0.24056187769195958 1103 74-75 0.24242498186033146 1103 76-77 0.42579141198735826 1103 78-79 0.3766385185065033 1103 80-81 0.2440057466358958 1103 82-83 0.08861764136688777 1103 84-85 0.33908528499917523 1103 86-87 0.32839622637746047 1103 88-89 0.4129676143082115 1103 90-91 0.43472829817879344 1103 92-93 0.2849503079220348 1103 94-95 0.06105504938821937 1103 96-97 0.40590285608278265 1103 98-99 0.5640789130956669 1103 100-101 0.8989148997167113 1104 1 0.4467478372288056 1104 2 0.49350722060273 1104 3 0.5936979953172354 1104 4 0.44060257941702474 1104 5 0.39150782537969064 1104 6 0.2916801503392463 1104 7 0.33253664712528774 1104 8 0.354416872014248 1104 9 0.5329887329212752 1104 10-11 0.48103922278951217 1104 12-13 0.4045730543398989 1104 14-15 0.4624058110736726 1104 16-17 0.7955310503167112 1104 18-19 0.7401886350300373 1104 20-21 0.6564415478313279 1104 22-23 0.4397112563499661 1104 24-25 0.8206715427570259 1104 26-27 0.8343961285439647 1104 28-29 0.6372031837426348 1104 30-31 0.7243287666964946 1104 32-33 0.9244198756070574 1104 34-35 0.8422209800111631 1104 36-37 0.5610884463043391 1104 38-39 0.5924390671793844 1104 40-41 0.784834445912395 1104 42-43 0.734732893287763 1104 44-45 0.5187061705607903 1104 46-47 0.5543030850701172 1104 48-49 1.102993274204458 1104 50-51 1.0147865569472785 1104 52-53 1.297737555613061 1104 54-55 1.3174090528684061 1104 56-57 1.2984172318418885 1104 58-59 1.2823605163584233 1104 60-61 1.47793754855131 1104 62-63 1.4195409134825638 1104 64-65 1.529778704771573 1104 66-67 1.3438986258954344 1104 68-69 1.0876963795846564 1104 70-71 1.3153981772458891 1104 72-73 1.461584924733181 1104 74-75 1.320749232728545 1104 76-77 1.296458938857274 1104 78-79 1.6634533872111206 1104 80-81 1.6121441386964044 1104 82-83 1.5042536655806984 1104 84-85 1.2664370028891163 1104 86-87 1.4761910404500185 1104 88-89 1.4857806161887623 1104 90-91 1.60023391924096 1104 92-93 1.5485671422825753 1104 94-95 1.6880745824076726 1104 96-97 1.9954932529201628 1104 98-99 2.0418760606983106 1104 100-101 2.0915718296147254 1105 1 0.5056597275728905 1105 2 0.505086184441943 1105 3 0.45815370578055337 1105 4 0.4160598702524325 1105 5 0.4691103539750401 1105 6 0.5166484646611735 1105 7 0.29775281386236685 1105 8 0.311113111980454 1105 9 0.4760718667521928 1105 10-11 0.6143694266731572 1105 12-13 0.5499633749520143 1105 14-15 0.7125466363095541 1105 16-17 0.5870353767809036 1105 18-19 0.5041381570065937 1105 20-21 0.47945160901207373 1105 22-23 0.38252447514544485 1105 24-25 0.6707591673482369 1105 26-27 0.5211695094449311 1105 28-29 0.45210797023592164 1105 30-31 0.2537886236806415 1105 32-33 0.5331917230776853 1105 34-35 0.4343989205203229 1105 36-37 0.3967571314660532 1105 38-39 0.27855526364623984 1105 40-41 0.3827627502200812 1105 42-43 0.644080559500658 1105 44-45 0.7582498967965279 1105 46-47 0.5252031147998935 1105 48-49 0.825700561128059 1105 50-51 0.4164866304893522 1105 52-53 0.5426013463154717 1105 54-55 0.5699243480537532 1105 56-57 0.5288738185511122 1105 58-59 0.345297661361478 1105 60-61 0.5178302084270712 1105 62-63 0.6816049420231494 1105 64-65 0.7626527913634504 1105 66-67 0.6185078358658629 1105 68-69 0.6214474944511039 1105 70-71 0.6104426936676788 1105 72-73 0.699082773236519 1105 74-75 0.8069092414909989 1105 76-77 0.8090980037777022 1105 78-79 0.5809884761657251 1105 80-81 0.6676957824398535 1105 82-83 0.9203687667401539 1105 84-85 0.8937234570661481 1105 86-87 0.7987316841778203 1105 88-89 1.0335125475286375 1105 90-91 1.0352430728812934 1105 92-93 1.0510472311589503 1105 94-95 1.1158195009637453 1105 96-97 1.19444410491284 1105 98-99 1.20192785309062 1105 100-101 1.309772708990085 1106 1 0.25886310639030796 1106 2 0.1349280731142386 1106 3 0.040297955293219445 1106 4 0.12063860102998802 1106 5 0.16416341873570417 1106 6 0.06331513132784039 1106 7 0.27252864232240626 1106 8 0.2576628195827908 1106 9 0.17005497266512037 1106 10-11 0.40370882455057 1106 12-13 0.32215960627105744 1106 14-15 0.3980632055103328 1106 16-17 0.3325692732504706 1106 18-19 0.26201773248851623 1106 20-21 0.2183166729063757 1106 22-23 0.3104517006165253 1106 24-25 0.39434374607901646 1106 26-27 0.41378376112351134 1106 28-29 0.33650476469119894 1106 30-31 0.41152308328644693 1106 32-33 0.27573666568110156 1106 34-35 0.42804847650906197 1106 36-37 0.4891612900105642 1106 38-39 0.4166535958966193 1106 40-41 0.37421607488977315 1106 42-43 0.5537903277484375 1106 44-45 0.7971972652175765 1106 46-47 0.641157197537602 1106 48-49 0.7086786854512113 1106 50-51 0.7595254002532599 1106 52-53 0.9289582880525273 1106 54-55 0.8372429532086088 1106 56-57 0.8953433875353056 1106 58-59 0.885700520360821 1106 60-61 0.9961364679029217 1106 62-63 1.073489282503978 1106 64-65 0.8381217105750522 1106 66-67 1.0531861984389579 1106 68-69 0.9055150707993889 1106 70-71 0.9806073027211752 1106 72-73 0.910854486470221 1106 74-75 0.8960103894225568 1106 76-77 1.0017100895476823 1106 78-79 1.1605336353600109 1106 80-81 1.2431127198450902 1106 82-83 1.396613081230086 1106 84-85 1.225038163585534 1106 86-87 1.3827169559993457 1106 88-89 1.2845218609356124 1106 90-91 0.9532937551425036 1106 92-93 1.1323814308557232 1106 94-95 1.1629475061619203 1106 96-97 1.4028456643670353 1106 98-99 1.0081678357633486 1106 100-101 0.6156943030988131 1107 1 0.13386093153167167 1107 2 0.17513685954312308 1107 3 0.10316441897307982 1107 4 0.19974690898997238 1107 5 0.15380239220242942 1107 6 0.1791828999228784 1107 7 0.2072263369722478 1107 8 0.25555755642489686 1107 9 0.3329997312558106 1107 10-11 0.5829780720494782 1107 12-13 0.4344649564232981 1107 14-15 0.2563559414824894 1107 16-17 0.26439397964455225 1107 18-19 0.1866110339239242 1107 20-21 0.31668335407210435 1107 22-23 0.2840141190593357 1107 24-25 0.5882845899241715 1107 26-27 0.3793557489442989 1107 28-29 0.35108936669207225 1107 30-31 0.34645348781015173 1107 32-33 0.3654452346241186 1107 34-35 0.36874660613063526 1107 36-37 0.13591640092835178 1107 38-39 0.04972232142945643 1107 40-41 0.15678240807811505 1107 42-43 0.14318354218949736 1107 44-45 0.3047375001023127 1107 46-47 0.45921069906000156 1107 48-49 0.5751989116365124 1107 50-51 0.7935314898574362 1107 52-53 0.9292540688267792 1107 54-55 0.9109184643003871 1107 56-57 0.9437209429941973 1107 58-59 0.9890585020920142 1107 60-61 1.2944814004823044 1107 62-63 1.3708968510120272 1107 64-65 1.218643676647261 1107 66-67 0.9516116007878068 1107 68-69 0.8848517389159554 1107 70-71 0.9359792035476175 1107 72-73 1.098816643929986 1107 74-75 1.3880786799836784 1107 76-77 1.0465100025533367 1107 78-79 1.0179847010407386 1107 80-81 0.8387412539903742 1107 82-83 1.030893203022167 1107 84-85 0.7346793119108916 1107 86-87 0.8187891613060039 1107 88-89 0.5353831049547466 1107 90-91 0.6245834463125775 1107 92-93 0.7258720789635937 1107 94-95 0.4567796070753616 1107 96-97 0.24900486401905653 1107 98-99 0.28577984098300746 1107 100-101 0.03547246751812594 1108 1 -0.016804458959590818 1108 2 -0.06699901632428151 1108 3 -0.21461706053141683 1108 4 -0.16604760222871562 1108 5 -0.060438620884504246 1108 6 -0.0839454477367525 1108 7 -0.09996227316229778 1108 8 -0.09410836785126975 1108 9 -0.04635107586377529 1108 10-11 -0.007185560632883892 1108 12-13 0.04456729587367647 1108 14-15 0.0873094837895465 1108 16-17 -0.06203159417421489 1108 18-19 -0.004520774134391559 1108 20-21 0.15723121985281097 1108 22-23 0.1351709597889581 1108 24-25 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5 195.0 6 206.0 7 281.0 8 335.0 9 422.0 10 641.0 11 829.0 12 1307.0 13 1866.0 14 2219.0 15 3056.0 16 4266.0 17 6284.0 18 5203.0 19 2672.0 20 2183.0 21 2163.0 22 2277.0 23 2441.0 24 2763.0 25 3070.0 26 3482.0 27 3960.0 28 4684.0 29 5475.0 30 6685.0 31 8473.0 32 10664.0 33 14089.0 34 19783.0 35 30769.0 36 46546.0 37 82165.0 38 103558.0 39 22610.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.341254136713964 23.392337815130162 17.16494757104875 21.101460477107125 2 13.518127630848403 23.69689691354552 41.47647920636125 21.308496249244836 3 15.135195946078145 31.38732366809493 32.50229891804113 20.9751814677858 4 9.87503027011954 20.12773378928083 43.28027806926782 26.716957871331815 5 9.493960457326844 39.94930551987532 37.644076795286786 12.912657227511055 6 23.239472802139325 43.160838408032745 19.689425949350056 13.910262840477875 7 21.934910895158684 35.23090925104959 27.177221259896463 15.656958593895268 8 21.79321245158654 35.11850440033373 24.241349191008982 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29.654802251597978 35.35299813670975 18.10704871452535 34-35 17.626712475317735 29.021818204048195 35.251835504557434 18.099633816076636 36-37 17.45467216020931 28.650251243993985 34.770946168987564 19.124130426809142 38-39 16.99174596498607 29.80818018762616 34.95940227630905 18.240671571078725 40-41 16.7283681942889 29.454613921233964 35.746458071489 18.07055981298813 42-43 17.085563524064053 29.39853926072998 33.70996965555015 19.80592755965582 44-45 16.942677183836942 29.589688543750093 33.97887926608017 19.4887550063328 46-47 18.02835153952894 29.36102060652 33.78674605260745 18.82388180134361 48-49 17.707524667986206 29.693519144508983 33.12924912251532 19.469707064989493 50-51 17.446273171184963 29.67598852719168 33.17199187220485 19.70574642941851 52-53 17.486921364359358 30.599911729142953 32.40836396935216 19.504802937145527 54-55 17.959379791128207 29.99041468704687 32.5883523776222 19.46185314420272 56-57 18.113421184883208 30.219033795986643 31.58583989855946 20.08170512057069 58-59 17.716116174861384 29.612852348377867 32.83058545919147 19.840446017569278 60-61 18.470564211921534 30.672962996022406 31.921754005411877 18.934718786644183 62-63 17.762803959543792 30.482275955161686 33.085739577831255 18.66918050746327 64-65 17.96716725577483 29.720883772281066 32.79300846188362 19.518940510060485 66-67 17.79505896594098 30.116709156380487 31.65428657019362 20.433945307484915 68-69 17.54777062274843 29.846214424245687 32.89942550522035 19.706589447785543 70-71 18.850072691213352 29.017183004562003 32.5507462923813 19.58199801184335 72-73 17.882483844738307 29.372802914977775 32.339624256430014 20.405088983853908 74-75 17.567988926618057 30.529568350865784 31.68814050087 20.214302221646165 76-77 17.37225491910426 29.814094482508153 32.821256546679365 19.99239405170822 78-79 17.450428601654234 29.101373555479732 32.679515543765795 20.768682299100234 80-81 17.879740256563903 30.48818069533954 31.24429824025045 20.38778080784611 82-83 18.38203731220076 29.048850732852717 32.098153957160065 20.47095799778646 84-85 17.460987080065443 28.899029843263563 31.938626031112516 21.70135704555848 86-87 17.456340501292484 29.86820835259218 32.362565204950805 20.31288594116453 88-89 17.986830762458503 28.59411969699749 32.30547250906379 21.113577031480226 90-91 17.48738716927238 28.207131660100416 32.844716061519804 21.460765109107406 92-93 18.10627572695855 29.399844935075738 32.2450661716329 20.248813166332813 94-95 17.205830205059854 28.169379195656692 33.02723126398553 21.59755933529793 96-97 17.016361203718876 28.892359471960432 32.74405441830772 21.34722490601297 98-99 17.03972629382314 29.449223810088814 31.969532206590916 21.541517689497127 100-101 16.751420550115295 29.046503520028384 32.48640614582887 21.71566978402745 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1444.0 1 952.0 2 467.5 3 473.5 4 433.0 5 432.0 6 631.0 7 1207.5 8 1970.0 9 2236.0 10 1723.5 11 1189.0 12 1116.0 13 1100.0 14 1047.0 15 1038.5 16 1102.0 17 1155.0 18 1143.5 19 1179.5 20 1284.5 21 1451.0 22 1566.0 23 1678.0 24 1830.0 25 1956.0 26 2360.5 27 2830.5 28 2852.0 29 3567.5 30 5566.5 31 7236.5 32 10417.0 33 15085.0 34 17338.5 35 20050.0 36 26480.5 37 30119.5 38 28602.5 39 26628.0 40 23741.0 41 20286.5 42 16392.0 43 14986.5 44 15077.5 45 14128.0 46 12082.5 47 10718.0 48 12832.0 49 11256.0 50 6433.0 51 3614.0 52 2456.0 53 2038.0 54 1745.5 55 1524.0 56 1376.5 57 1284.5 58 1245.5 59 1181.0 60 1131.0 61 1066.5 62 800.5 63 476.5 64 251.5 65 163.5 66 119.5 67 81.5 68 51.5 69 40.5 70 36.5 71 27.5 72 26.0 73 25.0 74 19.5 75 17.5 76 12.5 77 8.0 78 6.5 79 2.5 80 1.0 81 1.5 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03569813073829114 2 0.032764037800897344 3 0.02371725124393315 4 0.04058828563394746 5 0.06406102913309779 6 0.06308299815396652 7 0.06186045943005245 8 0.06503906011222906 9 0.060882428450921185 10-11 0.06772864530484003 12-13 0.06552807560179469 14-15 0.059537635854615696 16-17 0.05061310317004291 18-19 0.043522378571341246 20-21 0.05366944997982811 22-23 0.029340929373937917 24-25 0.016871034390014306 26-27 0.013203418218272063 28-29 0.015281734048925999 30-31 0.012347641111532207 32-33 0.007579740088267296 34-35 0.0091690404293556 36-37 0.00489015489565632 38-39 0.008191009450224336 40-41 0.006601709109136032 42-43 0.0034231084269594238 44-45 7.33523234348448E-4 46-47 0.0030563468097852004 48-49 8.557771067398559E-4 50-51 0.004401139406090688 52-53 0.004034377788916464 54-55 0.006357201364353215 56-57 0.018460334731102607 58-59 0.00794650170544152 60-61 0.01699328826240571 62-63 0.011980879494357983 64-65 0.008191009450224336 66-67 0.015281734048925999 68-69 0.02542880545741286 70-71 0.015526241793708815 72-73 0.015281734048925999 74-75 0.018704842475885424 76-77 0.02310598188197611 78-79 0.03802095431372789 80-81 0.029340929373937917 82-83 0.0336198149076372 84-85 0.020049635072190913 86-87 0.020049635072190913 88-89 0.01894935022066824 90-91 0.018582588603494016 92-93 0.030318960353069184 94-95 0.01956061958262528 96-97 0.03716517720698803 98-99 0.05513649644852501 100-101 0.06173820555766103 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 408985.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.35831387459198 #Duplication Level Percentage of deduplicated Percentage of total 1 76.70309425114098 17.916549506705625 2 8.929992044550517 4.171791141484406 3 3.1832265628271155 2.2306441556536303 4 1.7501988862370723 1.6352677971074734 5 1.1828497257463468 1.3814687580229104 6 0.8332286563664532 1.1677689890827292 7 0.6374827283004648 1.0423365160091447 8 0.5108235983754135 0.9545582356321136 9 0.4375497215592681 0.9198381358729537 >10 4.39538583929992 22.03968360697825 >50 0.8269480383536407 13.533748181473648 >100 0.5788636268475484 24.785016565399708 >500 0.02198216304484361 3.4091714855068034 >1k 0.007327387681614538 3.5592992408034525 >5k 0.0010467696688020768 1.252857684267149 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5124 1.252857684267149 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3837 0.9381762167316651 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2590 0.6332750589874935 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1978 0.48363631918041 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1808 0.4420700025673313 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1663 0.406616379573823 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1518 0.37116275658031467 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1163 0.284362507182415 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 909 0.22225754000757972 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 844 0.2063645365966967 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 813 0.19878479650842942 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 798 0.19511718033668718 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 753 0.18411433182146045 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 751 0.18362531633189483 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 749 0.18313630084232918 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 706 0.1726224678166681 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 680 0.16626526645231487 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 663 0.162108634791007 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 641 0.15672946440578506 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 630 0.15403987921317408 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 613 0.14988324755186622 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 608 0.14866070882795213 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 566 0.13839138354707384 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 562 0.13741335256794257 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 555 0.1357017983544629 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 544 0.1330122131618519 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 526 0.12861107375576122 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 522 0.12763304277662996 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 510 0.12469894983923617 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 495 0.12103133366749393 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 492 0.12029781043314547 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 489 0.11956428719879701 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 489 0.11956428719879701 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 484 0.11834174847488294 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 483 0.11809724073010013 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 480 0.11736371749575167 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 480 0.11736371749575167 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 476 0.11638568651662042 No Hit GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT 475 0.1161411787718376 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 472 0.11540765553748915 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 463 0.1132070858344438 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 448 0.10953946966270156 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 442 0.10807242319400467 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 435 0.10636086898052495 No Hit CCATGTAATTATTGGATCAACATTCCTTATTGTTTGCCTACTACGACAAC 432 0.10562734574617651 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 428 0.10464931476704524 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 422 0.10318226829834835 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 421 0.10293776055356554 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 419 0.1024487450639999 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 418 0.10220423731921709 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 415 0.10147071408486863 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.22253872391408E-4 0.0 16-17 0.0 0.0 0.0 2.44507744782816E-4 0.0 18-19 0.0 0.0 0.0 4.89015489565632E-4 0.0 20-21 0.0 0.0 0.0 4.89015489565632E-4 0.0 22-23 0.0 0.0 0.0 0.0012225387239140802 0.0 24-25 0.0 0.0 0.0 0.002934092937393792 0.0 26-27 0.0 0.0 0.0 0.00550142425761336 0.0 28-29 0.0 0.0 0.0 0.009291294301747008 0.0 30-31 0.0 0.0 0.0 0.018582588603494016 0.0 32-33 0.0 0.0 0.0 0.029340929373937917 0.0 34-35 0.0 0.0 0.0 0.038998985292859153 0.0 36-37 0.0 0.0 0.0 0.05342494223504529 0.0 38-39 0.0 0.0 0.0 0.0717630230937565 0.0 40-41 0.0 0.0 0.0 0.09878112889225765 0.0 42-43 0.0 0.0 0.0 0.13839138354707384 0.0 44-45 0.0 0.0 0.0 0.17286697556145092 0.0 46-47 0.0 0.0 0.0 0.24304069831411912 0.0 48-49 0.0 0.0 0.0 0.3254398083059281 0.0 50-51 0.0 0.0 0.0 0.3621159700233505 0.0 52-53 0.0 0.0 0.0 0.418597259068181 0.0 54-55 0.0 0.0 0.0 0.5040527158697752 0.0 56-57 0.0 0.0 0.0 0.6492903162707679 0.0 58-59 0.0 0.0 0.0 0.8337714097094026 0.0 60-61 0.0 0.0 0.0 1.0468599092876267 0.0 62-63 0.0 0.0 0.0 1.259459393376285 0.0 64-65 0.0 0.0 0.0 1.4897856889616978 0.0 66-67 0.0 0.0 0.0 1.8056896952210961 0.0 68-69 0.0 0.0 0.0 2.1631600180935733 0.0 70-71 0.0 0.0 0.0 2.3493526657456876 0.0 72-73 0.0 0.0 0.0 2.595938726359157 0.0 74-75 0.0 0.0 0.0 2.879689964179615 0.0 76-77 0.0 0.0 0.0 3.1762778586011713 0.0 78-79 0.0 0.0 0.0 3.5211560326173332 0.0 80-81 0.0 0.0 0.0 4.036333850874726 0.0 82-83 0.0 0.0 0.0 4.534762888614496 0.0 84-85 0.0 0.0 0.0 5.365966967003679 0.0 86-87 0.0 0.0 0.0 6.449258530263946 0.0 88-89 0.0 0.0 0.0 7.500030563468098 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAAGCT 20 0.0020713082 71.26569 4 GTATGGT 60 2.3423127E-8 55.428867 1 TGGACTG 55 8.1514736E-7 51.842274 5 GGTACTC 80 2.2495988E-7 41.581825 8 CTTTGCG 70 4.296864E-6 40.723248 2 GTGGTAC 85 3.619807E-7 39.135838 6 TGGTACT 85 3.619807E-7 39.135838 7 CATATAG 110 1.6116246E-9 38.872192 3 GACTGGT 50 0.0016109146 38.01767 7 ACCTGTG 55 0.0025718198 34.561516 7 CATGGAT 55 0.0025749083 34.553062 4 TATCTAA 35 2.0531114E-4 33.92774 70-71 GGATATG 115 1.0294025E-7 33.05884 9 ACATATA 130 8.241841E-9 32.891857 2 GTGGCGA 30 0.0039483993 31.669764 20-21 CCGCCGA 30 0.00395313 31.662022 84-85 CTGTGCC 80 4.4764273E-4 29.701303 9 GCTTTGC 80 4.482848E-4 29.694038 1 GAGAGCG 65 0.0058264732 29.244362 9 GGACTGG 100 4.9297607E-5 28.51325 6 >>END_MODULE