##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937054_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 408985 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.2166656478844 33.0 31.0 34.0 31.0 34.0 2 32.29948531119723 33.0 31.0 34.0 30.0 34.0 3 32.409790090101104 34.0 31.0 34.0 31.0 34.0 4 36.098825140286316 37.0 35.0 37.0 35.0 37.0 5 36.01015196156338 37.0 35.0 37.0 35.0 37.0 6 36.072105333936456 37.0 35.0 37.0 35.0 37.0 7 36.02063400858222 37.0 35.0 37.0 35.0 37.0 8 36.00840862134308 37.0 35.0 37.0 35.0 37.0 9 37.69780309791312 39.0 37.0 39.0 35.0 39.0 10-11 37.598705331491374 39.0 37.0 39.0 35.0 39.0 12-13 37.56670171277675 39.0 37.0 39.0 35.0 39.0 14-15 38.71532452290426 40.0 38.0 41.0 34.5 41.0 16-17 38.65912197268848 40.0 38.0 41.0 34.5 41.0 18-19 38.75688594936245 40.0 38.0 41.0 35.0 41.0 20-21 38.856009389097395 40.0 38.0 41.0 35.0 41.0 22-23 38.75833954790518 40.0 38.0 41.0 35.0 41.0 24-25 38.66861254080223 40.0 38.0 41.0 34.0 41.0 26-27 38.47217868625989 40.0 38.0 41.0 34.0 41.0 28-29 38.32947051847867 40.0 38.0 41.0 34.0 41.0 30-31 38.23640964827561 40.0 37.5 41.0 34.0 41.0 32-33 38.057884763499885 40.0 37.0 41.0 34.0 41.0 34-35 37.909206939129795 40.0 37.0 41.0 33.0 41.0 36-37 37.72967957260046 40.0 37.0 41.0 33.0 41.0 38-39 37.57948824529017 40.0 37.0 41.0 33.0 41.0 40-41 37.37495384916318 40.0 36.0 41.0 33.0 41.0 42-43 37.114922307664095 40.0 36.0 41.0 32.5 41.0 44-45 36.80467865569642 40.0 36.0 41.0 31.5 41.0 46-47 36.36721151142463 40.0 36.0 41.0 30.5 41.0 48-49 35.931668643104274 39.0 35.0 41.0 29.5 41.0 50-51 35.67070919471374 39.0 35.0 41.0 28.0 41.0 52-53 35.518792865264004 39.0 35.0 41.0 27.5 41.0 54-55 35.29699622235535 39.0 35.0 40.0 27.0 41.0 56-57 35.07499174786361 39.0 35.0 40.0 26.0 41.0 58-59 34.781824516791566 38.0 35.0 40.0 26.0 41.0 60-61 34.48410455151167 38.0 34.0 40.0 24.5 41.0 62-63 34.172490433634486 38.0 34.0 40.0 23.0 41.0 64-65 33.79208895191755 37.5 34.0 40.0 22.0 41.0 66-67 33.38527207599301 37.0 33.0 39.5 21.5 41.0 68-69 32.938190887196356 36.5 33.0 39.0 19.5 40.0 70-71 32.53348411310928 36.0 32.0 39.0 18.0 40.0 72-73 32.11931733437657 35.5 32.0 38.0 14.5 40.0 74-75 31.62983238994095 35.0 31.5 37.5 10.0 39.5 76-77 30.092469161460688 33.5 29.5 36.0 7.5 38.5 78-79 30.740546719317337 34.5 31.0 36.0 6.0 39.0 80-81 30.35531254202477 35.0 31.0 36.0 2.0 38.5 82-83 30.15948262161204 34.0 31.0 36.0 2.0 37.0 84-85 29.74769734831351 34.0 30.0 35.0 2.0 37.0 86-87 29.4048155800335 34.0 30.0 35.0 2.0 36.0 88-89 29.000388767314206 34.0 29.5 35.0 2.0 36.0 90-91 28.56202794723523 34.0 29.0 35.0 2.0 36.0 92-93 28.171329021846766 34.0 29.0 35.0 2.0 35.0 94-95 27.705275254593687 34.0 28.5 35.0 2.0 35.0 96-97 27.13370172500214 33.0 27.0 35.0 2.0 35.0 98-99 26.506347421054564 33.0 25.5 35.0 2.0 35.0 100-101 23.434290988667065 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3155485175674073 1101 2 0.2005154145451158 1101 3 0.10193180466784924 1101 4 0.16478045103733763 1101 5 0.28379798607106466 1101 6 0.23439509843554873 1101 7 0.32433367774642363 1101 8 0.12758192781230093 1101 9 0.3892865535414316 1101 10-11 0.3053775255379918 1101 12-13 0.16898474023481214 1101 14-15 0.3386098110050071 1101 16-17 0.43846044965167863 1101 18-19 0.5517198451382654 1101 20-21 0.35645100631523263 1101 22-23 0.31488700380720047 1101 24-25 0.31957439510767216 1101 26-27 0.17868241585500755 1101 28-29 0.19436731235668248 1101 30-31 0.2695303059947136 1101 32-33 0.4732686571251534 1101 34-35 0.4590425730351342 1101 36-37 0.448999301399013 1101 38-39 0.29569242626893555 1101 40-41 0.48672185664982237 1101 42-43 0.443298600817954 1101 44-45 0.24089824873168908 1101 46-47 0.3371311579907186 1101 48-49 0.2511471370116922 1101 50-51 0.16004811955362896 1101 52-53 0.0624419166938921 1101 54-55 0.204983461101655 1101 56-57 0.4265352895768899 1101 58-59 0.36381535454257374 1101 60-61 0.23874887705490977 1101 62-63 0.2857654905817313 1101 64-65 0.7539655615247156 1101 66-67 0.34416838465400446 1101 68-69 0.35150179354118904 1101 70-71 0.5139958638040838 1101 72-73 0.5429670526267429 1101 74-75 0.3770861050904344 1101 76-77 0.4335371412853206 1101 78-79 0.547311624229593 1101 80-81 0.7803002983417677 1101 82-83 0.5465512640967525 1101 84-85 0.47304556979409185 1101 86-87 0.217862885557512 1101 88-89 0.28253879802764104 1101 90-91 0.15529445546069098 1101 92-93 0.10796659422913635 1101 94-95 0.2234298902189522 1101 96-97 -0.06000446810823945 1101 98-99 -0.23411562417076937 1101 100-101 0.27316185059908804 1102 1 0.3381400872392675 1102 2 0.36239810015042906 1102 3 0.15903379717130406 1102 4 0.1961607364322262 1102 5 0.1958680853674437 1102 6 0.2902180746964831 1102 7 0.21748814608337597 1102 8 0.26131310424420917 1102 9 0.3812507805421532 1102 10-11 0.18645203070008876 1102 12-13 0.2586786312060809 1102 14-15 0.42085647372612556 1102 16-17 0.5470436679064292 1102 18-19 0.4537994795171727 1102 20-21 0.24149079062509315 1102 22-23 0.26103187130243555 1102 24-25 0.3823119501809913 1102 26-27 0.4375697703627992 1102 28-29 0.3394238652599455 1102 30-31 0.6087063433459363 1102 32-33 0.5347370598344341 1102 34-35 0.46763894312785226 1102 36-37 0.44392269194283784 1102 38-39 0.4452090962235644 1102 40-41 0.7489892335157435 1102 42-43 0.6408375631379428 1102 44-45 0.7681209093485037 1102 46-47 0.9606877437735832 1102 48-49 1.313059414252443 1102 50-51 1.1463948677616926 1102 52-53 1.2038833581353359 1102 54-55 1.2329426246447568 1102 56-57 1.2311055532712416 1102 58-59 1.4106769972071405 1102 60-61 1.3197329633309707 1102 62-63 1.2340393118482567 1102 64-65 1.340935378056578 1102 66-67 1.5020397596786665 1102 68-69 1.4581747073308975 1102 70-71 1.3975116788892379 1102 72-73 1.6349197717692405 1102 74-75 1.5224730979226848 1102 76-77 1.3233329590008474 1102 78-79 1.3685025231577193 1102 80-81 1.4982755728863886 1102 82-83 1.4218159448358882 1102 84-85 1.1954332859774333 1102 86-87 1.2909624449709405 1102 88-89 1.1652786546726048 1102 90-91 1.4198749118294707 1102 92-93 1.4154335502362407 1102 94-95 1.5219059209942536 1102 96-97 1.6598383674280264 1102 98-99 1.7618385416739066 1102 100-101 2.5316776656842492 1103 1 0.17300852968893565 1103 2 0.12343169692409361 1103 3 0.10042320101558033 1103 4 0.12261860224027288 1103 5 0.04454925751431915 1103 6 0.17531580780865852 1103 7 0.14749107319821775 1103 8 0.24912004475873317 1103 9 0.1160118954476701 1103 10-11 0.17605264214185468 1103 12-13 0.13370383691720633 1103 14-15 0.19804286589446463 1103 16-17 0.2991056576940494 1103 18-19 0.38099400256007243 1103 20-21 0.4789838793817154 1103 22-23 0.34226256047536197 1103 24-25 0.3543645081541271 1103 26-27 0.2234464412575221 1103 28-29 0.22055047923854687 1103 30-31 0.2446350518378182 1103 32-33 0.3263346140673704 1103 34-35 0.3454855623102091 1103 36-37 0.09123625098925459 1103 38-39 0.1824615110933081 1103 40-41 0.26711132672808446 1103 42-43 0.2873689320519759 1103 44-45 0.22211316875592502 1103 46-47 0.030644162285959453 1103 48-49 -0.21464247536280823 1103 50-51 0.07457485280088605 1103 52-53 -0.3761862421508724 1103 54-55 -0.2508948229110999 1103 56-57 -0.288338620144053 1103 58-59 -0.21005022821322683 1103 60-61 -0.18075163679261408 1103 62-63 -0.07943517346738105 1103 64-65 -0.15717175434658515 1103 66-67 -0.10845882554915676 1103 68-69 2.178334895432954E-4 1103 70-71 -0.10459421762098486 1103 72-73 -0.06425250520086578 1103 74-75 -0.2861066009097222 1103 76-77 -0.22401312354379144 1103 78-79 -0.0692263609610606 1103 80-81 0.405843790923921 1103 82-83 0.2882045355927083 1103 84-85 0.35212011721299774 1103 86-87 0.3686936057345953 1103 88-89 0.12473160943241979 1103 90-91 0.38081093810845346 1103 92-93 0.29707566166174715 1103 94-95 0.2151589212869638 1103 96-97 0.6548167393017081 1103 98-99 1.0130549205076171 1103 100-101 1.2064670760332241 1104 1 0.22786707658602978 1104 2 0.13761818715758523 1104 3 -0.015182624573633063 1104 4 0.12671600653121828 1104 5 0.11205004679655417 1104 6 0.16280396857515456 1104 7 0.24491595411954847 1104 8 0.35276996033984886 1104 9 0.1657984460784121 1104 10-11 0.16539390032636447 1104 12-13 0.36923938207100804 1104 14-15 0.39761802615307573 1104 16-17 0.34671653468955554 1104 18-19 0.30419340956889585 1104 20-21 0.4041599236364277 1104 22-23 0.28489546874017435 1104 24-25 0.3594914110106444 1104 26-27 0.34421348632209714 1104 28-29 0.19908200248462293 1104 30-31 0.21484822667412828 1104 32-33 0.4022395433065 1104 34-35 0.33642940659511567 1104 36-37 0.31985356578245927 1104 38-39 0.3818959732146183 1104 40-41 0.49832297790651126 1104 42-43 0.6706340897025242 1104 44-45 0.46363353223755865 1104 46-47 0.7875315076895859 1104 48-49 0.6304385804909245 1104 50-51 0.47745882144921836 1104 52-53 0.5947613083288772 1104 54-55 0.49276689758689685 1104 56-57 0.4727593743927372 1104 58-59 0.5502138724854575 1104 60-61 0.34770713522149066 1104 62-63 0.4797026558005726 1104 64-65 0.7390547902444311 1104 66-67 0.435936584259899 1104 68-69 0.599742891484631 1104 70-71 0.5822391820578119 1104 72-73 0.7615323158722802 1104 74-75 1.0735349963229837 1104 76-77 1.1623782780841907 1104 78-79 1.348441207088797 1104 80-81 1.5514903846563293 1104 82-83 1.1117381864691538 1104 84-85 0.9356055875565765 1104 86-87 0.9950096480675761 1104 88-89 0.8393683337052487 1104 90-91 0.8369817525104537 1104 92-93 1.06270001822514 1104 94-95 1.3228616295545237 1104 96-97 1.4304135053035445 1104 98-99 1.5062156183703763 1104 100-101 0.9549983247463487 1105 1 0.2654674145665936 1105 2 0.3156291089369887 1105 3 0.028481022174084103 1105 4 0.09076645326016575 1105 5 0.23637525920794644 1105 6 0.16212331326838836 1105 7 0.05759491952348128 1105 8 0.0965149727794099 1105 9 0.1548688430491083 1105 10-11 0.026931398957231067 1105 12-13 0.13051713637791806 1105 14-15 0.34210550053069966 1105 16-17 0.26272601692210884 1105 18-19 0.338445236385148 1105 20-21 0.2721714713057679 1105 22-23 0.18515599542655536 1105 24-25 0.3549067672202497 1105 26-27 0.3005772222591361 1105 28-29 0.24157601741210044 1105 30-31 0.24658867011715557 1105 32-33 0.34051506005688026 1105 34-35 0.33094113420781923 1105 36-37 0.5606060419595238 1105 38-39 0.44255644357090773 1105 40-41 0.7288969189789754 1105 42-43 0.6558748226196442 1105 44-45 0.049619074180000666 1105 46-47 0.08343271096299532 1105 48-49 0.31937128167542284 1105 50-51 0.38324282683186794 1105 52-53 0.3628534393276368 1105 54-55 0.3972013277923523 1105 56-57 0.5391858557959921 1105 58-59 0.69685206115998 1105 60-61 0.8299709791245462 1105 62-63 0.6921140532563328 1105 64-65 0.8540151244696546 1105 66-67 0.8427415726026979 1105 68-69 0.6745132681138983 1105 70-71 0.6938079751855426 1105 72-73 0.6203941350213853 1105 74-75 0.6577250165079391 1105 76-77 0.47281577515033035 1105 78-79 0.5009627611735112 1105 80-81 0.7085691998466785 1105 82-83 0.35508810588582307 1105 84-85 0.5390769296210713 1105 86-87 0.6918911514552057 1105 88-89 0.4647614708220864 1105 90-91 0.6816867236412811 1105 92-93 0.69660917030075 1105 94-95 1.039990672412337 1105 96-97 1.052931460521119 1105 98-99 1.5138905937259572 1105 100-101 1.3197435093056455 1106 1 0.1157598122274166 1106 2 0.09715390859044248 1106 3 0.0482514358626247 1106 4 0.12166097351141048 1106 5 0.10860397915596565 1106 6 0.12081077265759887 1106 7 0.09691915604069834 1106 8 0.17837332235706072 1106 9 0.2688562807792323 1106 10-11 0.26245013406661144 1106 12-13 0.22313571987368874 1106 14-15 0.3501215282543342 1106 16-17 0.3415434308272438 1106 18-19 0.278560029540877 1106 20-21 0.24926698787497514 1106 22-23 0.15501954318484223 1106 24-25 0.2346685172744003 1106 26-27 0.3366309367923819 1106 28-29 0.36159551058948836 1106 30-31 0.17157025989406094 1106 32-33 0.09495517268413067 1106 34-35 0.21113823189029546 1106 36-37 0.3448425591784954 1106 38-39 0.3810554689120451 1106 40-41 0.4567180149287253 1106 42-43 0.43719602686481807 1106 44-45 0.41851446079468957 1106 46-47 0.5332529405493034 1106 48-49 0.6378009979413903 1106 50-51 0.3778410507868202 1106 52-53 0.5045471798517909 1106 54-55 0.7073096232222795 1106 56-57 0.7105460463959474 1106 58-59 0.6282967221952873 1106 60-61 0.7397803791678612 1106 62-63 0.7062725977657536 1106 64-65 0.44702357151319205 1106 66-67 0.1708875548422526 1106 68-69 0.4957564996495307 1106 70-71 0.24944150345064742 1106 72-73 0.2658348974212146 1106 74-75 0.5356262510758398 1106 76-77 0.40043110978320584 1106 78-79 0.47662011443320296 1106 80-81 0.8160090958830644 1106 82-83 0.6372735076618703 1106 84-85 0.3972445709466079 1106 86-87 0.5047414871710352 1106 88-89 0.0820259282619844 1106 90-91 0.3150005637972271 1106 92-93 0.7130722415591428 1106 94-95 0.9807963904110366 1106 96-97 1.051294033620536 1106 98-99 0.7363857204316133 1106 100-101 0.8299232797193348 1107 1 0.1991221436758721 1107 2 0.1609425123312036 1107 3 0.08178340628455061 1107 4 0.08106506224564214 1107 5 0.14013507963444027 1107 6 0.14442103799035522 1107 7 0.10330454099067765 1107 8 0.11750772862065162 1107 9 0.04466750304978717 1107 10-11 0.16568849857291923 1107 12-13 0.16723532839913702 1107 14-15 0.18604149345659238 1107 16-17 0.14953603630788592 1107 18-19 0.24257916829685655 1107 20-21 0.2798237516853277 1107 22-23 0.2210069290047585 1107 24-25 0.3362692132291656 1107 26-27 0.16519987981108386 1107 28-29 0.42461856409101273 1107 30-31 0.20709440082490005 1107 32-33 0.3130391222274085 1107 34-35 0.2067276185801603 1107 36-37 0.3466737901484862 1107 38-39 0.4102638203691953 1107 40-41 0.15563058561163245 1107 42-43 0.16597158145377477 1107 44-45 0.4578554786285238 1107 46-47 0.9414021358516109 1107 48-49 1.1723029553141586 1107 50-51 0.9481129081160944 1107 52-53 0.7938468753715782 1107 54-55 0.8470703887725222 1107 56-57 0.8399544848474392 1107 58-59 1.1447995495115109 1107 60-61 1.1274576301465444 1107 62-63 1.0373339287792405 1107 64-65 1.0081958203170203 1107 66-67 0.9775534965560411 1107 68-69 0.8656629807282599 1107 70-71 0.7862857835724384 1107 72-73 0.9773942710619252 1107 74-75 1.0262460857865818 1107 76-77 0.9735172341851239 1107 78-79 0.8630729200008425 1107 80-81 1.0753174908373957 1107 82-83 0.8165036077053678 1107 84-85 0.9601436575059843 1107 86-87 1.0719076463706863 1107 88-89 0.8142921309588083 1107 90-91 0.8051180061634753 1107 92-93 0.6561583659610584 1107 94-95 0.8016859075924216 1107 96-97 0.8540104320546362 1107 98-99 0.8493283041636701 1107 100-101 0.8522699521856225 1108 1 -0.08766299869744643 1108 2 -0.047541906671540346 1108 3 -0.21479392810408626 1108 4 -0.07931429316307259 1108 5 -0.11158591651862793 1108 6 -0.14524010062806525 1108 7 -0.037541047921322956 1108 8 -0.11290788734871882 1108 9 -0.017164115422851012 1108 10-11 -0.042264436323726784 1108 12-13 -0.00417174701178169 1108 14-15 0.011042873934790975 1108 16-17 -0.15431871897934712 1108 18-19 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66-67 -0.8979667843956705 2316 68-69 -1.1521191216070008 2316 70-71 -0.768751436526415 2316 72-73 -0.8166169950217608 2316 74-75 -1.1321219329435337 2316 76-77 -1.2641285945624006 2316 78-79 -0.9795746096651783 2316 80-81 -0.8586818688168201 2316 82-83 -1.1543156346855241 2316 84-85 -1.4141559642897867 2316 86-87 -1.1546672040485397 2316 88-89 -1.371658282264331 2316 90-91 -1.3139863101673441 2316 92-93 -1.6426254773143114 2316 94-95 -1.4167695583591922 2316 96-97 -1.2058794084592392 2316 98-99 -1.6553767365583205 2316 100-101 -2.3827694691018735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 9.0 6 29.0 7 55.0 8 119.0 9 228.0 10 446.0 11 751.0 12 1198.0 13 1642.0 14 2017.0 15 2863.0 16 4094.0 17 5866.0 18 5066.0 19 2822.0 20 2207.0 21 2181.0 22 2317.0 23 2569.0 24 2969.0 25 3316.0 26 3765.0 27 4726.0 28 5877.0 29 6941.0 30 8386.0 31 10517.0 32 13430.0 33 17520.0 34 23849.0 35 34526.0 36 53321.0 37 91474.0 38 84715.0 39 7171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.134486275488115 24.067973204000324 17.16132629926113 19.63621422125043 2 14.20998063342397 25.446751697020677 40.71162385805669 19.631643811498662 3 16.896324897723634 32.086046642425195 31.124294213590716 19.893334246260462 4 11.362763915547024 21.030355636514784 42.17147328141619 25.435407166522 5 10.391531648726763 40.81177040185055 36.838257229348734 11.958440720073945 6 25.585045906329086 43.371272785065464 17.996748046994387 13.046933261611063 7 24.18254948225485 35.46413682653398 25.832487744049292 14.520825947161875 8 23.90650023839505 35.38173771654217 22.928224751519004 17.783537293543773 9 26.284093548663158 19.030526791936133 23.76590828514493 30.91947137425578 10-11 21.697372764282306 29.722483709671504 30.372018533687058 18.208124992359135 12-13 24.213479711969875 28.43270535594215 29.54154797853222 17.812266953555753 14-15 21.85483575192244 30.184847855055807 28.701908382948034 19.25840801007372 16-17 18.016797682066578 32.37930486448158 30.510776678851304 19.093120774600536 18-19 17.656875426374974 31.377774952258232 33.44234676786373 17.523002853503062 20-21 18.995077962819426 30.063182037131675 33.812048110284934 17.129691889763972 22-23 19.293005855960487 29.050698680880714 34.15076347543308 17.50553198772571 24-25 19.207784491169214 30.31503764362406 33.46492504285187 17.01225282235486 26-27 18.431567210164417 30.15617817075676 34.54735961814963 16.864895000929195 28-29 17.742712452103063 29.428586798907446 35.09332413907779 17.7353766099117 30-31 18.820095215151266 30.000232351383776 33.87829923678685 17.301373196678107 32-33 18.262817127320385 31.120043815895 33.642147370068265 16.97499168671635 34-35 18.85591536673721 29.29850879477314 34.541873438892615 17.303702399597032 36-37 18.716620040691758 29.485386180452306 33.50565380702715 18.29233997182878 38-39 17.91160288543832 31.028365325834457 33.95097200146717 17.109059787260055 40-41 18.22486697445127 30.370050667084 34.2729077819946 17.132174576470128 42-43 18.168491271485056 30.71241505622978 32.52621977909118 18.592873893193985 44-45 18.487206948152927 30.371272360944022 32.739405142650355 18.40211554825269 46-47 19.33683555492526 30.732789131947037 32.15132335807384 17.779051955053873 48-49 19.099554863310882 31.068364730894636 31.58783350102879 18.24424690476569 50-51 18.729047922947707 31.028390904934117 31.807912091136494 18.43464908098169 52-53 19.03459557398675 31.269645461692036 31.331141640493094 18.36461732382812 54-55 19.341181418401394 31.07076358061444 31.16649082033232 18.42156418065185 56-57 19.30605493415945 31.354795644291112 30.61038362464714 18.728765796902298 58-59 19.107366845892596 30.342729857866257 31.86737281193992 18.68253048430123 60-61 19.171240999058643 32.05545435651674 30.48119124173258 18.29211340269203 62-63 18.81389293005856 31.503233614924753 32.06364536596697 17.61922808904972 64-65 18.787397877964626 31.183502996720847 31.41739638632879 18.611702738985734 66-67 19.08008626254328 31.211612190207934 30.320012518827145 19.38828902842165 68-69 18.483322513720125 30.89604735689686 31.587402302560395 19.033227826822614 70-71 19.50193416503245 30.72058039622263 31.146659102801728 18.630826335943194 72-73 18.782134285088958 30.856739014277558 30.864686613919055 19.496440086714426 74-75 18.92423897867013 32.07806688722775 29.954127206527826 19.043566927574293 76-77 19.16367233012973 30.80851365576398 31.188373619844167 18.839440394262123 78-79 18.862860796916898 30.364101203481695 30.711219413188047 20.06181858641336 80-81 19.24256205621736 32.30004107740156 29.19111750092913 19.26627936545195 82-83 19.69747726555833 30.460606268079353 30.26706703084157 19.574849435520747 84-85 18.695810573570476 30.77812715474031 29.660963074169523 20.865099197519697 86-87 18.25103610156852 31.59639106568701 30.177757130457106 19.974815702287373 88-89 18.64547891881847 30.387294197616743 30.416024314236566 20.55120256932822 90-91 18.65040020245464 29.722933000473127 30.781051908342818 20.845614888729415 92-93 18.851494008927013 31.357499752435004 29.998153959756323 19.792852278881668 94-95 18.153166039498167 29.90652377494309 30.782970358036195 21.157339827522552 96-97 18.08862704730142 30.531305052648822 30.139235445928747 21.24083245412101 98-99 18.015518963478964 31.388796858552976 29.63848174804118 20.95720242992688 100-101 17.28594744273899 31.019786910197872 29.81337832303338 21.880887324029757 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1613.0 1 1081.0 2 513.5 3 489.0 4 474.0 5 470.5 6 620.5 7 1002.0 8 1472.0 9 1655.5 10 1507.0 11 1265.5 12 1163.5 13 1146.5 14 1121.0 15 1075.0 16 1026.0 17 1042.5 18 1056.0 19 1119.0 20 1203.5 21 1308.0 22 1439.5 23 1540.0 24 1652.0 25 1871.0 26 2219.5 27 2560.0 28 2670.5 29 3611.5 30 5581.5 31 6945.5 32 9794.0 33 13822.5 34 16341.5 35 19546.5 36 25763.0 37 29322.0 38 28668.0 39 27390.5 40 24702.5 41 20960.5 42 16560.0 43 15421.5 44 16033.5 45 14744.0 46 12192.5 47 11105.5 48 13142.5 49 11576.0 50 6849.5 51 3914.5 52 2623.5 53 2121.0 54 1727.5 55 1545.0 56 1411.0 57 1329.5 58 1341.5 59 1333.5 60 1310.0 61 1241.5 62 967.0 63 562.5 64 279.0 65 182.0 66 120.5 67 82.5 68 50.0 69 35.0 70 29.0 71 24.0 72 20.5 73 23.0 74 21.0 75 17.0 76 11.5 77 6.0 78 5.0 79 3.0 80 1.0 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028851913884372287 2 0.008068755577832928 3 0.010758340770443903 4 0.0 5 0.006601709109136032 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0034231084269594238 20-21 0.0019560619582625277 22-23 0.0 24-25 0.004401139406090688 26-27 0.007090724598701663 28-29 0.008313263322615744 30-31 0.029707690991112146 32-33 2.4450774478281597E-4 34-35 0.005012408768047728 36-37 0.013936941452620512 38-39 0.00855777106739856 40-41 0.010024817536095456 42-43 0.009291294301747008 44-45 0.0031786006821766075 46-47 0.0015893003410883037 48-49 0.002322823575436752 50-51 0.0039121239165250555 52-53 0.004034377788916464 54-55 0.002567331320219568 56-57 0.001100284851522672 58-59 9.780309791312639E-4 60-61 0.0 62-63 0.0 64-65 0.009291294301747008 66-67 2.4450774478281597E-4 68-69 0.0013447925963054879 70-71 0.005623678130004767 72-73 0.013814687580229103 74-75 0.0068462168539188475 76-77 0.0045233932784820964 78-79 0.011369610132400943 80-81 2.4450774478281597E-4 82-83 0.005868185874787584 84-85 7.33523234348448E-4 86-87 0.0 88-89 0.0015893003410883037 90-91 0.001100284851522672 92-93 3.66761617174224E-4 94-95 9.780309791312639E-4 96-97 3.66761617174224E-4 98-99 3.66761617174224E-4 100-101 6.1126936195704E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 408985.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.655598616086163 #Duplication Level Percentage of deduplicated Percentage of total 1 75.24336808478492 17.046835458513147 2 9.688316173455071 4.389892049830679 3 3.5107599991366096 2.3861510813355014 4 1.8670810939152582 1.6919935938970867 5 1.2249347061235942 1.3875814516424807 6 0.8623108636059487 1.17217012848882 7 0.6691273284551793 1.0611636123574213 8 0.545015001403009 0.9878112889225765 9 0.4306158129897041 0.8780273115150922 >10 4.506896328433594 22.226487523992322 >50 0.831012972436271 13.116862476618946 >100 0.5806298430788491 24.35003728743108 >500 0.032377128796218346 4.97524359084074 >1k 0.007554663385784283 4.329743144614106 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3963 0.9689841925742997 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3235 0.7909825543724097 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2857 0.6985586268445053 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2571 0.6286294118366199 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2103 0.514199787278262 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1756 0.42935559983862487 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1223 0.29903297186938393 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 980 0.2396175898871597 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 959 0.23448292724672057 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 925 0.22616966392410479 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 924 0.225925156179322 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 873 0.21345526119539837 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 867 0.21198821472670146 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 837 0.204652982383217 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 827 0.20220790493538882 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 788 0.192672102888859 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 738 0.1804467156497182 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 709 0.17335599105101654 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 699 0.17091091360318839 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 688 0.1682213284105774 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 665 0.16259765028057263 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 657 0.16064158832231012 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 615 0.15037226304143184 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 595 0.14548210814577553 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 574 0.14034744550533637 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 559 0.13667982933359415 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 557 0.1361908138440285 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 552 0.13496827512011444 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 551 0.13472376737533162 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 550 0.1344792596305488 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 542 0.13252319767228626 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 542 0.13252319767228626 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 533 0.13032262796924093 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 520 0.1271440272870643 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 514 0.12567698081836742 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 507 0.12396542660488771 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 501 0.1224983801361908 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 494 0.12078682592271112 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 488 0.1193197794540142 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 476 0.11638568651662042 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 469 0.11467413230314069 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 466 0.11394060906879226 No Hit GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT 464 0.11345159357922661 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 463 0.1132070858344438 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 451 0.11027299289705002 No Hit ATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGGAG 449 0.10978397740748438 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 431 0.1053828380013937 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 428 0.10464931476704524 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 427 0.10440480702226243 No Hit ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT 422 0.10318226829834835 No Hit TACTAGAGTAGCTCCTCCGATTAGGTGTATTAATAAGTGTCCTGCAGTAA 418 0.10220423731921709 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 414 0.10122620634008582 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 412 0.10073719085052019 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.22253872391408E-4 0.0 16-17 0.0 0.0 0.0 2.44507744782816E-4 0.0 18-19 0.0 0.0 0.0 4.89015489565632E-4 0.0 20-21 0.0 0.0 0.0 4.89015489565632E-4 0.0 22-23 0.0 0.0 0.0 0.0012225387239140802 0.0 24-25 0.0 0.0 0.0 0.002934092937393792 0.0 26-27 0.0 0.0 0.0 0.005623678130004767 0.0 28-29 0.0 0.0 0.0 0.009535802046529825 0.0 30-31 0.0 0.0 0.0 0.018827096348276832 0.0 32-33 0.0 0.0 0.0 0.02982994486350355 0.0 34-35 0.0 0.0 0.0 0.039365746910033375 0.0 36-37 0.0 0.0 0.0 0.05379170385221951 0.0 38-39 0.0 0.0 0.0 0.07237429245571353 0.0 40-41 0.0 0.0 0.0 0.0993923982542147 0.0 42-43 0.0 0.0 0.0 0.1387581451642481 0.0 44-45 0.0 0.0 0.0 0.17396726041297358 0.0 46-47 0.0 0.0 0.0 0.2447522525275988 0.0 48-49 0.0 0.0 0.0 0.3266623470298422 0.0 50-51 0.0 0.0 0.0 0.363460762619656 0.0 52-53 0.0 0.0 0.0 0.4189640206853552 0.0 54-55 0.0 0.0 0.0 0.504297223614558 0.0 56-57 0.0 0.0 0.0 0.6501460933775077 0.0 58-59 0.0 2.44507744782816E-4 0.0 0.8356052177952737 0.0 60-61 0.0 2.44507744782816E-4 0.0 1.050527525459369 0.0 62-63 0.0 2.44507744782816E-4 0.0 1.2644718021443329 0.0 64-65 0.0 2.44507744782816E-4 0.0 1.496142890326051 0.0 66-67 0.0 2.44507744782816E-4 0.0 1.8153477511400173 0.0 68-69 0.0 2.44507744782816E-4 0.0 2.1730625817572773 0.0 70-71 0.0 2.44507744782816E-4 0.0 2.356076628727215 0.0 72-73 0.0 2.44507744782816E-4 0.0 2.6002176118928566 0.0 74-75 0.0 2.44507744782816E-4 0.0 2.879812218052007 0.0 76-77 0.0 2.44507744782816E-4 0.0 3.1739550350257346 0.0 78-79 0.0 2.44507744782816E-4 0.0 3.5176106703179824 0.0 80-81 0.0 2.44507744782816E-4 0.0 4.030098903382765 0.0 82-83 0.0 2.44507744782816E-4 0.0 4.530728510825581 0.0 84-85 0.0 2.44507744782816E-4 0.0 5.3641331589178085 0.0 86-87 0.0 2.44507744782816E-4 0.0 6.445468660219813 0.0 88-89 0.0 2.44507744782816E-4 0.0 7.495996185679181 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCCTA 25 0.005020708 56.995014 7 CGGGTCG 45 1.5150217E-5 52.77316 6 GTATGGT 60 1.487042E-6 47.513268 1 GGTACTC 130 0.0 47.501648 3 CCGGGTC 55 4.960241E-5 43.17804 5 GGGTCGG 55 4.960241E-5 43.17804 7 TGGGGTC 55 4.960241E-5 43.17804 6 GTGGTAC 155 0.0 39.84984 1 TGGTACT 155 0.0 39.840096 2 GGTCGGG 60 8.285654E-5 39.57987 8 GTATTAC 50 0.0016123838 38.010616 1 TACTCCC 175 0.0 37.996674 5 GTCGGGA 65 1.3273724E-4 36.535263 9 CTCCCGC 175 0.0 35.282627 7 TCGTTAG 55 0.0025787975 34.54243 9 TGGGATA 55 0.0025787975 34.54243 6 CCCGCTG 190 0.0 32.497158 9 GGATATG 105 1.8935789E-6 31.675512 1 CTCGTTA 60 0.003949695 31.663895 8 GGGTATG 60 0.003949695 31.663895 7 >>END_MODULE