##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937053_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 435264 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05484257829731 33.0 31.0 34.0 30.0 34.0 2 32.192005311718866 33.0 31.0 34.0 30.0 34.0 3 32.16443813409793 34.0 31.0 34.0 30.0 34.0 4 35.76025354727246 37.0 35.0 37.0 35.0 37.0 5 35.76517010366123 37.0 35.0 37.0 35.0 37.0 6 35.874738089986764 37.0 35.0 37.0 35.0 37.0 7 35.83300479708866 37.0 35.0 37.0 35.0 37.0 8 35.79705190413175 37.0 35.0 37.0 35.0 37.0 9 37.458055341126304 39.0 37.0 39.0 35.0 39.0 10-11 37.35443432032054 39.0 37.0 39.0 34.5 39.0 12-13 37.31451946404941 39.0 37.0 39.0 34.0 39.0 14-15 38.47945040251433 40.0 38.0 41.0 34.0 41.0 16-17 38.421704988237025 40.0 38.0 41.0 34.0 41.0 18-19 38.45844820614616 40.0 38.0 41.0 34.0 41.0 20-21 38.39489597118071 40.0 38.0 41.0 34.0 41.0 22-23 38.27471603440671 40.0 38.0 41.0 34.0 41.0 24-25 38.357186672915745 40.0 38.0 41.0 34.0 41.0 26-27 38.29837179275107 40.0 38.0 41.0 34.0 41.0 28-29 38.20897891854139 40.0 38.0 41.0 34.0 41.0 30-31 37.993200678209085 40.0 37.5 41.0 34.0 41.0 32-33 37.69889997794442 40.0 36.5 41.0 33.0 41.0 34-35 37.49847104286134 40.0 36.0 41.0 33.0 41.0 36-37 37.31754636266726 40.0 36.0 41.0 33.0 41.0 38-39 37.14394827047493 40.0 36.0 41.0 33.0 41.0 40-41 36.97818450411704 40.0 36.0 41.0 32.0 41.0 42-43 36.709021421482134 39.0 35.0 41.0 31.0 41.0 44-45 36.35221612630495 39.0 35.0 41.0 30.5 41.0 46-47 35.882171509704456 39.0 35.0 41.0 29.5 41.0 48-49 35.513982318776655 39.0 35.0 41.0 27.0 41.0 50-51 34.75132678098809 38.5 34.0 40.0 25.5 40.5 52-53 34.96608380201441 38.5 35.0 40.0 26.5 40.5 54-55 35.40302092523159 39.0 35.0 41.0 26.5 41.0 56-57 35.33263146044699 39.0 35.0 41.0 26.0 41.0 58-59 35.135702699970594 39.0 35.0 41.0 25.0 41.0 60-61 34.91623474489046 39.0 35.0 41.0 24.0 41.0 62-63 34.60742216218203 39.0 35.0 40.5 22.5 41.0 64-65 34.31342472614321 38.0 35.0 40.0 22.0 41.0 66-67 33.961996627334216 38.0 34.0 40.0 20.5 41.0 68-69 33.55786488200265 37.0 34.0 40.0 18.5 41.0 70-71 33.15578821129246 37.0 34.0 39.0 15.0 41.0 72-73 32.72292677547419 36.0 33.5 39.0 10.5 41.0 74-75 32.24962781208646 36.0 33.0 38.5 7.5 40.0 76-77 31.81334776135862 35.0 32.5 37.5 2.0 39.5 78-79 31.350207000808705 35.0 32.0 37.0 2.0 39.0 80-81 30.868386772165856 35.0 31.5 36.5 2.0 39.0 82-83 30.422584913983236 35.0 31.0 36.0 2.0 38.0 84-85 29.953265144831644 35.0 31.0 36.0 2.0 37.0 86-87 29.636775612042346 35.0 31.0 35.0 2.0 37.0 88-89 29.262634860682255 34.0 30.5 35.0 2.0 36.0 90-91 28.87710791611528 34.0 30.0 35.0 2.0 36.0 92-93 28.542412191221878 34.0 29.0 35.0 2.0 36.0 94-95 28.210206908910457 34.0 29.0 35.0 2.0 35.0 96-97 27.78685234708131 34.0 29.0 35.0 2.0 35.0 98-99 27.29276025584473 34.0 28.0 35.0 2.0 35.0 100-101 24.97729768600206 31.5 22.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45942707796841376 1101 2 0.5060558971591576 1101 3 0.2921919077962869 1101 4 0.30769294654378854 1101 5 0.23391182978733838 1101 6 0.213311414239854 1101 7 0.1266649062196592 1101 8 0.15799048241015612 1101 9 0.3463821942778722 1101 10-11 0.3685469307104867 1101 12-13 0.3607575942401908 1101 14-15 0.43191516306313105 1101 16-17 0.45138969339875956 1101 18-19 0.5336821483294614 1101 20-21 0.5134853434452964 1101 22-23 0.496287997295191 1101 24-25 0.5377213965353036 1101 26-27 0.5262607740963219 1101 28-29 0.48002880041774176 1101 30-31 0.47404604056119126 1101 32-33 0.7709654618955426 1101 34-35 0.5443790579813808 1101 36-37 0.5051115620858795 1101 38-39 0.7776914991141552 1101 40-41 0.6365893150093669 1101 42-43 0.7254606221418101 1101 44-45 0.8264063912505293 1101 46-47 0.814573595453318 1101 48-49 0.7607791766402059 1101 50-51 0.7006288511678278 1101 52-53 0.650383368203137 1101 54-55 0.7814991361860848 1101 56-57 0.8832909754069718 1101 58-59 0.77982563098562 1101 60-61 0.8540555629621664 1101 62-63 0.769709608903085 1101 64-65 0.5614338811187878 1101 66-67 0.5198418093432338 1101 68-69 0.6453499629847812 1101 70-71 0.46447283176829046 1101 72-73 0.5157311438659846 1101 74-75 0.3985855047615452 1101 76-77 0.1555824581452967 1101 78-79 0.3017619767011297 1101 80-81 0.49922107388970005 1101 82-83 0.4749614748672215 1101 84-85 0.39253764309637873 1101 86-87 0.5749453307903032 1101 88-89 0.2705973019796204 1101 90-91 0.3547981637139763 1101 92-93 0.3872512109882784 1101 94-95 0.3512486544748299 1101 96-97 0.3488398119151732 1101 98-99 0.3988572327191804 1101 100-101 0.6821882528855703 1102 1 0.3594805123412854 1102 2 0.3062842076614416 1102 3 0.33685818584933713 1102 4 0.1741677352351374 1102 5 0.37406776100272765 1102 6 0.2459549583960765 1102 7 0.3750375866821045 1102 8 0.2339523011219029 1102 9 0.36577644279628174 1102 10-11 0.4443569472265665 1102 12-13 0.3430392419619466 1102 14-15 0.3647153670852745 1102 16-17 0.626648364340177 1102 18-19 0.5036617461143678 1102 20-21 0.6073233887187826 1102 22-23 0.668874949592869 1102 24-25 0.37511695662468014 1102 26-27 0.39928734555265066 1102 28-29 0.3933497466919107 1102 30-31 0.3810945201103948 1102 32-33 0.6414903331672832 1102 34-35 0.5602697118237927 1102 36-37 0.09537314762945215 1102 38-39 0.41101025943670777 1102 40-41 0.44888286402326827 1102 42-43 0.4567525195478197 1102 44-45 0.28814009376679905 1102 46-47 0.5428233768581592 1102 48-49 0.6984249067480448 1102 50-51 0.7061787394414054 1102 52-53 0.8531000202967931 1102 54-55 0.9623137674889151 1102 56-57 1.1363634518472736 1102 58-59 1.046244556468956 1102 60-61 0.924431060873367 1102 62-63 0.9130898187544432 1102 64-65 1.0309702164923422 1102 66-67 1.0427275083619847 1102 68-69 1.2600395578550803 1102 70-71 1.3664146854552541 1102 72-73 1.4629525564765018 1102 74-75 1.4135714175177903 1102 76-77 1.287607863760769 1102 78-79 1.2489165963455484 1102 80-81 1.142892743956736 1102 82-83 1.2651465521041807 1102 84-85 1.375991203768681 1102 86-87 1.2339004459170866 1102 88-89 1.5732423034369276 1102 90-91 1.1838628193051584 1102 92-93 1.1850567584477183 1102 94-95 0.9918903527163536 1102 96-97 0.9105322242342275 1102 98-99 0.6886779846865387 1102 100-101 0.5966325354076716 1103 1 0.39297213176752877 1103 2 0.5678182330362986 1103 3 0.4429834895377667 1103 4 0.4926540784582798 1103 5 0.5200958843922621 1103 6 0.5422444378925064 1103 7 0.4803407843065912 1103 8 0.5357633885105528 1103 9 0.5248969120102558 1103 10-11 0.46686363450724144 1103 12-13 0.5527161665490539 1103 14-15 0.7150981985658049 1103 16-17 0.7592681832314412 1103 18-19 0.8801709856048987 1103 20-21 0.8011625013350425 1103 22-23 0.8033843854072487 1103 24-25 0.5594987819455497 1103 26-27 0.7168595559136435 1103 28-29 0.7487725791540569 1103 30-31 0.7871474293842411 1103 32-33 0.9981156491259497 1103 34-35 0.8711761144348955 1103 36-37 1.1124165106567716 1103 38-39 1.3190540151116892 1103 40-41 1.2325063885269358 1103 42-43 1.1553647554309094 1103 44-45 1.121086914547682 1103 46-47 1.4964418705635225 1103 48-49 1.562335677564505 1103 50-51 1.5822707907800933 1103 52-53 1.4912997307191347 1103 54-55 1.3548448725467068 1103 56-57 1.4431869321780795 1103 58-59 1.4713102022516722 1103 60-61 1.5762139267105724 1103 62-63 1.5198132966311988 1103 64-65 1.5854746215084177 1103 66-67 1.6600187302324585 1103 68-69 1.6158611731094439 1103 70-71 1.5977056373804572 1103 72-73 1.4674956127478183 1103 74-75 1.452366848080505 1103 76-77 1.3950092723842928 1103 78-79 1.547451529718149 1103 80-81 1.7693927807674612 1103 82-83 1.6312751687573481 1103 84-85 1.604821972621906 1103 86-87 1.5307328835229193 1103 88-89 1.6951778110567446 1103 90-91 1.8257804123749217 1103 92-93 1.8386157414902478 1103 94-95 1.7245126256555707 1103 96-97 1.966812746455627 1103 98-99 1.7769500895234671 1103 100-101 2.0567972020252547 1104 1 0.3321873900633605 1104 2 0.3868346096404167 1104 3 0.29623923033676647 1104 4 0.2971298794738786 1104 5 0.3778967587526978 1104 6 0.1964740441735202 1104 7 0.10010633758672327 1104 8 0.20464270494300507 1104 9 0.29139388513105047 1104 10-11 0.31140947423349985 1104 12-13 0.3772518631806463 1104 14-15 0.34675460444366735 1104 16-17 0.265681443722805 1104 18-19 0.27028532010876205 1104 20-21 0.5419277914082983 1104 22-23 0.48146952320568204 1104 24-25 0.5551703125898939 1104 26-27 0.280891050864561 1104 28-29 0.47447087986471104 1104 30-31 0.41800619000078854 1104 32-33 0.5324609380436556 1104 34-35 0.23809935431908968 1104 36-37 0.44697974771629845 1104 38-39 0.47260153037448305 1104 40-41 0.388538320016103 1104 42-43 0.599550451099546 1104 44-45 0.7711003958760472 1104 46-47 0.7795995186494977 1104 48-49 0.7552085486380733 1104 50-51 0.5237602207766869 1104 52-53 0.39318936357761913 1104 54-55 0.2647157727784517 1104 56-57 0.36315919915973893 1104 58-59 0.36625534608460697 1104 60-61 0.3258672639813085 1104 62-63 0.6402095834881933 1104 64-65 0.3851196131949308 1104 66-67 0.3819326294820016 1104 68-69 0.519962385787025 1104 70-71 0.5378758231018068 1104 72-73 0.7423526143719954 1104 74-75 0.6595170878555514 1104 76-77 0.5993027544830127 1104 78-79 0.44195532053928943 1104 80-81 0.5867801635280472 1104 82-83 0.37870890160877835 1104 84-85 0.5548800707274602 1104 86-87 0.9113813996138447 1104 88-89 1.0202383165423612 1104 90-91 0.8250357294750259 1104 92-93 0.6862590768992796 1104 94-95 0.905236252004503 1104 96-97 0.7178800182841449 1104 98-99 0.8182125838259999 1104 100-101 0.7808520646120023 1105 1 0.35244853054013703 1105 2 0.27074961646647466 1105 3 0.34958399877587 1105 4 0.5325378677120511 1105 5 0.3711372110227984 1105 6 0.2852162992230518 1105 7 0.29425343038610663 1105 8 0.38096153436156754 1105 9 0.5588983483228489 1105 10-11 0.4825912574099007 1105 12-13 0.5184339964112965 1105 14-15 0.5349682775629958 1105 16-17 0.6666423426621364 1105 18-19 0.5788423964555918 1105 20-21 0.5163090705065585 1105 22-23 0.5717887726893522 1105 24-25 0.46844962088268005 1105 26-27 0.5696473080844342 1105 28-29 0.4441894362409542 1105 30-31 0.3179204267074738 1105 32-33 0.5957489976929082 1105 34-35 0.17928148754973705 1105 36-37 0.5583197554174859 1105 38-39 0.8808383070748675 1105 40-41 0.8478357611194767 1105 42-43 0.9987838322736309 1105 44-45 0.9470254142538934 1105 46-47 1.0675524013463118 1105 48-49 0.9036035498632558 1105 50-51 0.9379899263810572 1105 52-53 0.8682077414261897 1105 54-55 1.0070548353331503 1105 56-57 1.0447603473377072 1105 58-59 1.031906271978123 1105 60-61 0.918523125639858 1105 62-63 0.7584631627929852 1105 64-65 0.9087167707543529 1105 66-67 0.9456915121088016 1105 68-69 1.095537349906465 1105 70-71 0.9032974634560631 1105 72-73 0.9130495724004888 1105 74-75 0.9585299348468403 1105 76-77 0.8404848877136644 1105 78-79 0.8707752876341885 1105 80-81 0.9217365817728762 1105 82-83 1.0393838062189928 1105 84-85 1.0063170434582318 1105 86-87 0.8532601408187297 1105 88-89 0.5652535439025996 1105 90-91 0.7869673288729331 1105 92-93 0.6863045839528716 1105 94-95 0.796001820667648 1105 96-97 0.6605288788575372 1105 98-99 0.4485535366736926 1105 100-101 0.6958028819886941 1106 1 0.16844976022157 1106 2 0.3274994147218635 1106 3 0.4397311762727014 1106 4 0.4493768178715243 1106 5 0.5412905369268088 1106 6 0.38217283766981325 1106 7 0.44862456361440906 1106 8 0.5489629177531796 1106 9 0.3215621457328339 1106 10-11 0.4954702490711185 1106 12-13 0.5613825419287224 1106 14-15 0.5715013444653536 1106 16-17 0.4117428306919564 1106 18-19 0.5035263298990813 1106 20-21 0.46266998200792386 1106 22-23 0.4138573274293904 1106 24-25 0.5226574370453037 1106 26-27 0.3451497482335313 1106 28-29 0.4050298466471318 1106 30-31 0.369311503831554 1106 32-33 0.5780704056781474 1106 34-35 0.5546129251241965 1106 36-37 0.3102949312235097 1106 38-39 0.46265439284515253 1106 40-41 0.4773654079703462 1106 42-43 0.42711261834749337 1106 44-45 0.5439070189881647 1106 46-47 0.9502311854987937 1106 48-49 0.9448824185563396 1106 50-51 0.6005883969647741 1106 52-53 0.7313807038056268 1106 54-55 0.9568646189860743 1106 56-57 1.0260768750247493 1106 58-59 0.886771199157927 1106 60-61 0.9957394342899093 1106 62-63 1.114269564821953 1106 64-65 0.8223931339715094 1106 66-67 0.8677950359146749 1106 68-69 0.8735779293938464 1106 70-71 0.9555781382437516 1106 72-73 1.0047492227098331 1106 74-75 1.2841803210436673 1106 76-77 1.3241666307870972 1106 78-79 1.3417471202437987 1106 80-81 1.3800119535593751 1106 82-83 1.182526410837987 1106 84-85 1.2369476394694523 1106 86-87 1.137879515986853 1106 88-89 1.0309166111892338 1106 90-91 1.0712543057779804 1106 92-93 1.0700920027746825 1106 94-95 1.0265478760801656 1106 96-97 0.8232176541716818 1106 98-99 0.8567674243884049 1106 100-101 0.8672627444180847 1107 1 0.15649165722476965 1107 2 0.2202297858927338 1107 3 0.059133478945298634 1107 4 -0.007508339450055246 1107 5 0.10906558992152782 1107 6 0.26419204046294453 1107 7 -0.034587539964299197 1107 8 0.24954066412667686 1107 9 0.36579091667093877 1107 10-11 0.3194799751611441 1107 12-13 0.27521104685411757 1107 14-15 0.33664328718949577 1107 16-17 0.38664619325897576 1107 18-19 0.3062778989584771 1107 20-21 0.23868103816154473 1107 22-23 0.33035635066393354 1107 24-25 0.30275843874944997 1107 26-27 0.2839522753543591 1107 28-29 0.21009240120051942 1107 30-31 0.2799727948245305 1107 32-33 0.3201232757059955 1107 34-35 0.2840177216660251 1107 36-37 0.5102450538387444 1107 38-39 0.2941228661815387 1107 40-41 0.575829600164532 1107 42-43 0.61476380917005 1107 44-45 1.0279649598834624 1107 46-47 0.964664453714434 1107 48-49 0.7584553018848226 1107 50-51 0.8313668998119397 1107 52-53 0.8261207179375418 1107 54-55 0.8151795946708518 1107 56-57 0.908892037749716 1107 58-59 0.9464037690901748 1107 60-61 1.351099174927505 1107 62-63 1.1008774870132356 1107 64-65 1.3363255501151556 1107 66-67 0.9698732602797762 1107 68-69 0.9636544080504805 1107 70-71 0.929712557795682 1107 72-73 0.82602608375975 1107 74-75 0.740778683402862 1107 76-77 0.7452211218299567 1107 78-79 0.5690425190550563 1107 80-81 0.6390065086115371 1107 82-83 0.41572188862176773 1107 84-85 0.6734329464231941 1107 86-87 0.5847580229904672 1107 88-89 0.4737633629245508 1107 90-91 0.4848687535937657 1107 92-93 0.2370939563055856 1107 94-95 0.4203568456965243 1107 96-97 0.6432974579872983 1107 98-99 0.7126467211172809 1107 100-101 0.6764921388235052 1108 1 -0.08063927030281448 1108 2 0.24243591260229636 1108 3 0.14554649926824936 1108 4 0.2688241559698241 1108 5 0.4150218764027187 1108 6 0.19485115170660805 1108 7 0.2789227493288706 1108 8 0.29509475215019876 1108 9 0.29873369712341713 1108 10-11 0.28602805577126134 1108 12-13 0.3584840220149559 1108 14-15 0.4285673637094547 1108 16-17 0.4512663628299407 1108 18-19 0.4611030022774685 1108 20-21 0.44376603667033976 1108 22-23 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18 5671.0 19 2686.0 20 2368.0 21 2218.0 22 2487.0 23 2701.0 24 2993.0 25 3371.0 26 3864.0 27 4329.0 28 5073.0 29 6125.0 30 7568.0 31 9384.0 32 11829.0 33 15743.0 34 22394.0 35 33401.0 36 51266.0 37 88478.0 38 105726.0 39 21232.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.29385323883849 23.401033192694115 17.104900311612386 21.20021325685501 2 13.503378774266631 23.70426706677359 41.48505228426279 21.30730187469699 3 15.033604852885421 31.31558690731954 32.599119954963754 21.051688284831286 4 9.856721013868302 20.13121459676215 43.29254420736962 26.71952018199993 5 9.700993317348916 39.76027971963669 37.72605958065043 12.812667382363962 6 23.399452961592388 43.289125887788174 19.53731583423357 13.774105316385867 7 22.117927660679367 35.10164315634917 27.156082619675136 15.624346563296331 8 21.814662939943364 35.23546394027435 24.185392225368687 18.764480894413595 9 24.255913736323084 17.485079206032207 25.009479903753125 33.24952715389159 10-11 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35.018747243052495 18.272473717100425 36-37 17.634470114689016 28.635609652992205 34.604515880017644 19.125404352301132 38-39 17.079923034950173 29.867034260934496 34.796818012119125 18.25622469199621 40-41 16.832772754006765 29.49210134539038 35.60310524187619 18.072020658726657 42-43 17.285831127775328 29.377688023820024 33.559632774591975 19.776848073812676 44-45 17.003933245111014 29.530928356124097 33.95916041758565 19.50597798117924 46-47 18.102942240734635 29.346476329855363 33.64915734951357 18.90142407989643 48-49 17.83606753228254 29.85191558579557 32.965927144444045 19.346089737477843 50-51 17.521340521316397 29.767026180692845 32.989442027645325 19.722191270345434 52-53 17.576175296516467 30.677120128657993 32.24559892019184 19.501105654633697 54-55 18.107525350053013 29.948501512330118 32.494247631563525 19.44972550605334 56-57 18.30219499979323 30.120594033019195 31.501371587687416 20.075839379500163 58-59 17.82961409183957 29.526672624805002 32.76083894876384 19.882874334591587 60-61 18.54756535634241 30.700967378405668 31.80263688444647 18.948830380805447 62-63 17.925666221714724 30.546716507410633 32.912581877666554 18.61503539320809 64-65 18.12252231140274 29.6697717108989 32.63409529257834 19.573610685120027 66-67 17.901033869894846 30.23382991409884 31.622471476380916 20.2426647396254 68-69 17.56851463353546 29.816474762120315 32.887828326811416 19.727182277532805 70-71 18.92387202926726 29.093188283903466 32.363694440231455 19.61924524659782 72-73 18.01130769168904 29.340599116938442 32.295856642091216 20.35223654928129 74-75 17.628026894494717 30.61133043186529 31.620448456048273 20.14019421759172 76-77 17.501108886953283 29.880653887079685 32.65137743294394 19.96685979302309 78-79 17.47523398740271 29.113364110365303 32.70538383585379 20.7060180663782 80-81 18.048961569271047 30.63402255574289 31.067300608772904 20.249715266213162 82-83 18.581347405097183 29.19469642820206 31.92313084488041 20.30082532182035 84-85 17.608041748338042 28.912585395894563 31.801166876159005 21.678205979608393 86-87 17.603501858351667 29.845759683821715 32.25891692852096 20.291821529305658 88-89 18.084413383623982 28.61569357783198 32.222167330915305 21.07772570762873 90-91 17.640746111227287 28.30560043018363 32.62501637317511 21.42863708541397 92-93 18.21262483668242 29.43221214450776 32.152275759362105 20.202887259447714 94-95 17.30807011416472 28.165954166374657 32.943822961389415 21.582152758071203 96-97 17.052719142228163 28.9423311135316 32.65015832747361 21.354791416766627 98-99 17.037016608867713 29.502974979833567 31.917086657060317 21.5429217542384 100-101 16.85799097410485 29.075012382737487 32.43576203538877 21.63123460776889 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1433.0 1 969.0 2 492.0 3 474.0 4 412.5 5 401.5 6 600.0 7 1213.0 8 2042.5 9 2252.5 10 1723.0 11 1234.5 12 1144.0 13 1141.0 14 1083.5 15 1075.0 16 1124.0 17 1157.5 18 1168.5 19 1246.5 20 1368.0 21 1487.5 22 1597.5 23 1782.5 24 1979.5 25 2102.0 26 2440.0 27 2914.5 28 3052.0 29 3783.5 30 5760.0 31 7533.5 32 10993.5 33 16062.5 34 18474.0 35 21259.0 36 28251.5 37 32212.5 38 30649.5 39 28576.5 40 25576.0 41 21919.0 42 17613.0 43 15959.5 44 16133.0 45 15307.0 46 12929.5 47 11361.5 48 13567.5 49 11992.5 50 6914.0 51 3780.5 52 2559.0 53 2135.5 54 1875.5 55 1670.0 56 1457.5 57 1318.0 58 1275.0 59 1243.5 60 1207.0 61 1132.5 62 896.5 63 541.0 64 278.5 65 183.0 66 119.5 67 87.0 68 57.5 69 41.0 70 31.0 71 22.5 72 23.5 73 26.5 74 25.5 75 21.0 76 13.5 77 5.0 78 3.0 79 3.0 80 1.5 81 1.5 82 1.0 83 1.0 84 0.5 85 1.0 86 1.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024812527569475076 2 0.010798044405234524 3 0.013554991912953979 4 0.022744816938685488 5 0.059044625790324955 6 0.04457065137479782 7 0.014933465666813703 8 0.047787090133803854 9 0.030556168210557268 10-11 0.02366379944125864 12-13 0.03721879135421262 14-15 0.020102742243787677 16-17 0.01929863255403617 18-19 0.016426812233495073 20-21 0.013325246287310688 22-23 0.007581605646228495 24-25 0.001837965005146302 26-27 1.1487281282164388E-4 28-29 0.002871820320541097 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.446184384649316E-4 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.1487281282164388E-4 48-49 0.0012636009410380827 50-51 1.1487281282164388E-4 52-53 3.446184384649316E-4 54-55 0.001952837817967946 56-57 4.594912512865755E-4 58-59 0.0011487281282164387 60-61 0.00356105719747096 62-63 0.0025272018820761653 64-65 0.0010338553153947949 66-67 0.012521136597559183 68-69 0.014933465666813703 70-71 0.023434053815615353 72-73 0.01688630348478165 74-75 0.018839141302749596 76-77 0.032853624466990146 78-79 0.020906851933539184 80-81 0.04790196294662549 82-83 0.03698904572856933 84-85 0.01952837817967946 86-87 0.015278084105278634 88-89 0.01389961035141891 90-91 0.023204308189972062 92-93 0.03342798853109837 94-95 0.013669864725775622 96-97 0.020677106307895897 98-99 0.03101565946184385 100-101 0.04192857667990001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 435264.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.016654851509518 #Duplication Level Percentage of deduplicated Percentage of total 1 76.64699999749014 17.641575443458816 2 9.016999999704732 4.150823535785305 3 3.311999999891546 2.2869348259710987 4 1.7090000032038637 1.5734185285988795 5 1.1649999999765241 1.3407201450734128 6 0.8079999999736086 1.115847427164735 7 0.6219999999796325 1.0021451522019091 8 0.5219999999829067 0.9611755065675631 9 0.4329999999858211 0.8969590395339544 >10 4.309999999858865 21.513897455549973 >50 0.8199999999731484 13.249997697934605 >100 0.5949999999805163 24.875249729954355 >500 0.03199999999895214 4.781710045244522 >1k 0.00699999999977078 3.4115285819790278 >5k 9.999999999672543E-4 1.1980168849818407 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5205 1.1958259814733128 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3833 0.880614983090722 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2718 0.6244486104984561 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1966 0.4516799000147037 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1874 0.4305433024555213 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1677 0.38528341420379353 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1559 0.35817343037788557 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1195 0.27454602264372885 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 930 0.2136634318482576 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 870 0.19987869430966038 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 856 0.1966622555506543 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 853 0.19597301867372444 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 819 0.18816166740185267 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 778 0.17874209675047786 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 747 0.17161998235553597 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 730 0.16771430671960005 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 708 0.16265990295544772 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 702 0.161281429201588 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 692 0.15898397294515512 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 682 0.15668651668872224 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 679 0.1559972798117924 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 668 0.1534700779297162 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 660 0.1516321129245699 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 648 0.14887516541685047 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 614 0.14106381414497868 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 597 0.1371581385090428 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 575 0.13210373474489046 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 566 0.13003602411410087 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 562 0.1291170416115277 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 561 0.12888729598588441 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 548 0.1259006028525217 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 545 0.12521136597559182 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 544 0.12498162034994854 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 537 0.12337340097044551 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 531 0.1219949272165858 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 524 0.12038670783708277 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 522 0.1199272165857962 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 510 0.11717026907807676 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 510 0.11717026907807676 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 507 0.1164810322011469 No Hit GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT 497 0.11418357594471401 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 496 0.11395383031907072 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 496 0.11395383031907072 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 490 0.11257535656521099 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 475 0.10912917218056169 No Hit GCTAATGCCATTGGTTGAATAAATAGGCTAATTGTTTCAATAATAATAAG 462 0.10614247904719894 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 446 0.10246654903690634 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 443 0.10177731215997649 No Hit CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT 441 0.1013178209086899 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.1487281282164388E-4 0.0 22-23 0.0 0.0 0.0 4.594912512865755E-4 0.0 24-25 0.0 0.0 0.0 0.0014933465666813702 0.0 26-27 0.0 0.0 0.0 0.002986693133362741 0.0 28-29 0.0 0.0 0.0 0.008615460961623292 0.0 30-31 0.0 0.0 0.0 0.018839141302749593 0.0 32-33 0.0 0.0 0.0 0.02986693133362741 0.0 34-35 0.0 0.0 0.0 0.0384823922952507 0.0 36-37 0.0 0.0 0.0 0.05134814733127481 0.0 38-39 0.0 0.0 0.0 0.07317398176738715 0.0 40-41 0.0 0.0 0.0 0.09821625496250552 0.0 42-43 0.0 0.0 0.0 0.13554991912953976 0.0 44-45 0.0 0.0 0.0 0.16909278047345977 0.0 46-47 0.0 0.0 0.0 0.23284719158947215 0.0 48-49 0.0 0.0 0.0 0.3094673577415086 0.0 50-51 0.0 0.0 0.0 0.346111785031613 0.0 52-53 0.0 0.0 0.0 0.39677069548595795 0.0 54-55 0.0 0.0 0.0 0.4897028010586678 0.0 56-57 0.0 0.0 0.0 0.6444364799294222 0.0 58-59 0.0 0.0 0.0 0.826395015438906 0.0 60-61 0.0 0.0 0.0 1.0317876047640053 0.0 62-63 0.0 0.0 0.0 1.2395925231583589 0.0 64-65 0.0 0.0 0.0 1.4735884428760477 0.0 66-67 0.0 0.0 0.0 1.7721428833995 0.0 68-69 0.0 0.0 0.0 2.1175654315541834 0.0 70-71 0.0 0.0 0.0 2.3077948095868255 0.0 72-73 0.0 1.1487281282164388E-4 0.0 2.557068813409793 0.0 74-75 0.0 2.2974562564328775E-4 0.0 2.8287430157329805 0.0 76-77 0.0 2.2974562564328775E-4 0.0 3.1061608586972502 0.0 78-79 0.0 2.2974562564328775E-4 0.0 3.431136046169681 0.0 80-81 0.0 2.2974562564328775E-4 0.0 3.9243999044258198 0.0 82-83 0.0 2.2974562564328775E-4 0.0 4.408818556094692 0.0 84-85 0.0 2.2974562564328775E-4 0.0 5.179730002940744 0.0 86-87 0.0 2.2974562564328775E-4 0.0 6.187279444199382 0.0 88-89 0.0 2.2974562564328775E-4 0.0 7.214012645199235 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 40 5.3709577E-4 47.524204 1 GGTACTC 90 1.1870725E-8 42.248592 3 TACTCCC 95 1.9130312E-8 40.024986 5 TATACTC 105 4.6207788E-8 36.21308 5 GTGGTAC 105 4.6253263E-8 36.208916 1 CTAAGAG 55 0.002569988 34.56703 3 CTTAGGG 55 0.002569988 34.56703 3 GGTATAT 90 2.4011651E-5 31.682802 1 CTCCCGC 110 2.7248043E-6 30.232246 7 CATATAG 95 3.4710796E-5 30.01874 3 GTTGCAA 145 2.3890607E-8 29.48761 8 ACATATA 100 4.925116E-5 28.517801 2 ACTATAT 185 2.8921932E-10 28.247889 8 TGGTACT 135 4.1919702E-7 28.16573 2 GGTTGCA 170 3.621608E-9 27.945774 7 GGGTTGC 155 4.561298E-8 27.588356 6 TAAGACT 70 0.0083604315 27.159811 4 GAGATGG 210 4.1836756E-11 27.159811 5 ATGTATT 70 0.008365123 27.156689 1 GTTAGCA 35 0.008376689 27.152006 72-73 >>END_MODULE