##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937052_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 451350 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.99789520327905 33.0 31.0 34.0 30.0 34.0 2 32.11249141464496 33.0 31.0 34.0 30.0 34.0 3 32.097804364683725 34.0 31.0 34.0 30.0 34.0 4 35.71911155422621 37.0 35.0 37.0 35.0 37.0 5 35.727652597762265 37.0 35.0 37.0 35.0 37.0 6 35.817343524980615 37.0 35.0 37.0 35.0 37.0 7 35.776860529522544 37.0 35.0 37.0 35.0 37.0 8 35.74703888334995 37.0 35.0 37.0 35.0 37.0 9 37.41345075883461 39.0 37.0 39.0 35.0 39.0 10-11 37.30803478453528 39.0 37.0 39.0 34.0 39.0 12-13 37.27799047302537 39.0 37.0 39.0 34.0 39.0 14-15 38.413125069236735 40.0 38.0 41.0 34.0 41.0 16-17 38.363379860418746 40.0 38.0 41.0 34.0 41.0 18-19 38.40998559875928 40.0 38.0 41.0 34.0 41.0 20-21 38.37181344854326 40.0 38.0 41.0 34.0 41.0 22-23 38.26438683948156 40.0 38.0 41.0 34.0 41.0 24-25 38.22351944167497 40.0 38.0 41.0 34.0 41.0 26-27 38.2148576492744 40.0 38.0 41.0 34.0 41.0 28-29 38.14155865736125 40.0 38.0 41.0 34.0 41.0 30-31 37.94580037664784 40.0 37.5 41.0 34.0 41.0 32-33 37.66211255123518 40.0 36.5 41.0 33.0 41.0 34-35 37.49621801262878 40.0 36.0 41.0 33.0 41.0 36-37 37.3103655699568 40.0 36.0 41.0 33.0 41.0 38-39 37.141824526420734 40.0 36.0 41.0 33.0 41.0 40-41 36.98437243824084 40.0 36.0 41.0 32.0 41.0 42-43 36.723775340644735 39.0 35.0 41.0 31.0 41.0 44-45 36.38048631882131 39.0 35.0 41.0 30.5 41.0 46-47 35.92126398582032 39.0 35.0 41.0 29.5 41.0 48-49 35.56861637310291 39.0 35.0 41.0 27.0 41.0 50-51 34.80874930763266 38.5 34.5 40.0 25.5 40.5 52-53 35.01435028248588 38.5 35.0 40.0 26.5 40.5 54-55 35.44409881466711 39.0 35.0 41.0 26.5 41.0 56-57 35.35559543591448 39.0 35.0 41.0 26.0 41.0 58-59 35.1940268084635 39.0 35.0 41.0 26.0 41.0 60-61 34.96204608397031 39.0 35.0 41.0 24.5 41.0 62-63 34.681036889332006 39.0 35.0 40.5 23.0 41.0 64-65 34.398636313282374 38.5 35.0 40.0 22.5 41.0 66-67 34.03776005317381 38.0 34.0 40.0 21.5 41.0 68-69 33.647118644067795 37.0 34.0 40.0 20.0 41.0 70-71 33.21693918245264 37.0 34.0 39.0 18.0 41.0 72-73 32.79928880026587 36.0 33.5 39.0 13.5 41.0 74-75 32.37092167940622 36.0 33.0 39.0 9.0 40.0 76-77 31.926928104575165 35.0 33.0 37.5 6.5 39.5 78-79 31.439400686828403 35.0 32.0 37.0 2.0 39.0 80-81 30.957640412097042 35.0 32.0 36.5 2.0 39.0 82-83 30.523228093497288 35.0 31.0 36.0 2.0 38.0 84-85 30.075177799933535 35.0 31.0 36.0 2.0 37.0 86-87 29.75199401794616 35.0 31.0 35.0 2.0 37.0 88-89 29.37177799933533 34.0 30.5 35.0 2.0 36.0 90-91 29.00105018278498 34.0 30.0 35.0 2.0 36.0 92-93 28.655613160518442 34.0 29.5 35.0 2.0 36.0 94-95 28.344273845131273 34.0 29.0 35.0 2.0 35.0 96-97 27.940782098149995 34.0 29.0 35.0 2.0 35.0 98-99 27.528268527750082 34.0 29.0 35.0 2.0 35.0 100-101 25.510263653483992 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.43073415454801633 1101 2 0.25916894282002545 1101 3 0.367250740826357 1101 4 0.4743845561514348 1101 5 0.2045549791182637 1101 6 0.2919033921952803 1101 7 0.24370909594804147 1101 8 0.3692733061146214 1101 9 0.3156218174913832 1101 10-11 0.2970064379272159 1101 12-13 0.4127361551020883 1101 14-15 0.6501579806317608 1101 16-17 0.7554752431869431 1101 18-19 0.7673180940780426 1101 20-21 0.5825183033708115 1101 22-23 0.604660525303089 1101 24-25 0.6480709751813905 1101 26-27 0.6532732667582906 1101 28-29 0.4744518059569316 1101 30-31 0.6273495570847274 1101 32-33 0.733652690245826 1101 34-35 0.8915779671836432 1101 36-37 0.9284535642722105 1101 38-39 0.8226968500451122 1101 40-41 1.0846308911326545 1101 42-43 0.9262826496207381 1101 44-45 0.9813418637839746 1101 46-47 1.0042750534588194 1101 48-49 1.1333383232278607 1101 50-51 1.2941949553052083 1101 52-53 1.080683716115871 1101 54-55 1.3244269297644422 1101 56-57 1.331632763908992 1101 58-59 1.4056157260184463 1101 60-61 1.535657825687906 1101 62-63 1.569738271042219 1101 64-65 1.3858859100422691 1101 66-67 1.5551390878119875 1101 68-69 1.3032662483230624 1101 70-71 1.2974200655359098 1101 72-73 1.470381707205867 1101 74-75 1.36753968339913 1101 76-77 1.1180802310424092 1101 78-79 1.1857622750515304 1101 80-81 0.9116887250397525 1101 82-83 0.7640886119631425 1101 84-85 0.7182854098624425 1101 86-87 0.8368328010269863 1101 88-89 0.9251274103612168 1101 90-91 0.8480257353157938 1101 92-93 0.7434431126188521 1101 94-95 0.6639277002260151 1101 96-97 0.6722468843275209 1101 98-99 0.8852849392567883 1101 100-101 1.3379458956435926 1102 1 0.2327261136123404 1102 2 0.21083195451593184 1102 3 0.2757850975515055 1102 4 0.20428221697014948 1102 5 0.16206594403054453 1102 6 0.25919140389118667 1102 7 0.16823394974920802 1102 8 0.11973017745965109 1102 9 0.03720441983056588 1102 10-11 0.16227507827809262 1102 12-13 0.06866634516057246 1102 14-15 0.08263239583643411 1102 16-17 0.31527970225127433 1102 18-19 -0.011738923465820505 1102 20-21 0.09462539401408776 1102 22-23 -0.022715205691056894 1102 24-25 0.07090540500595921 1102 26-27 0.07615338371735447 1102 28-29 -0.08238298936470301 1102 30-31 0.17618910386835296 1102 32-33 -0.02173839162552582 1102 34-35 0.04380749934738759 1102 36-37 0.34210488006168305 1102 38-39 0.2416021863024227 1102 40-41 0.24234287943674815 1102 42-43 0.4427757051762953 1102 44-45 0.5104902555968422 1102 46-47 0.5810294394237232 1102 48-49 0.7887202676723035 1102 50-51 0.838968347118076 1102 52-53 1.0406160991568072 1102 54-55 0.9241619443843234 1102 56-57 0.8489025007511017 1102 58-59 0.8129165301120196 1102 60-61 0.9817470069744587 1102 62-63 0.8510796453112235 1102 64-65 1.103767854486712 1102 66-67 0.9791156959991199 1102 68-69 1.0607589383815466 1102 70-71 0.9167548608575515 1102 72-73 1.0486437686093808 1102 74-75 1.2323057652704819 1102 76-77 1.022776868469311 1102 78-79 1.010780549905327 1102 80-81 1.2342398946303952 1102 82-83 1.2554080564075853 1102 84-85 1.257941842610979 1102 86-87 1.1171690583369305 1102 88-89 1.0427260945717443 1102 90-91 0.8299336300526363 1102 92-93 1.054069940104231 1102 94-95 1.1499931242026236 1102 96-97 1.1332300714620231 1102 98-99 1.1453817959819368 1102 100-101 0.9697532786845287 1103 1 0.4549692528359053 1103 2 0.4512011694709912 1103 3 0.46258886022123136 1103 4 0.349996024581543 1103 5 0.22215451259049956 1103 6 0.3039119527533032 1103 7 0.3448391801948034 1103 8 0.4623297423860251 1103 9 0.21305365008491606 1103 10-11 0.37331118699868426 1103 12-13 0.451974876906597 1103 14-15 0.6885799957599446 1103 16-17 0.6821146013715662 1103 18-19 0.45318326528919783 1103 20-21 0.5314635203288702 1103 22-23 0.58390173827528 1103 24-25 0.5866155670828306 1103 26-27 0.6272745349891053 1103 28-29 0.5532816931749807 1103 30-31 0.6632280333959883 1103 32-33 0.5866355804532688 1103 34-35 0.6477351145701817 1103 36-37 0.7016950263611648 1103 38-39 0.790441890356739 1103 40-41 0.7556042582854801 1103 42-43 0.8241419439408801 1103 44-45 0.8030025667462084 1103 46-47 0.6558824227857443 1103 48-49 0.9853176691831109 1103 50-51 0.8929371987149395 1103 52-53 0.8900032310175803 1103 54-55 1.1004638783104994 1103 56-57 1.1928138604757166 1103 58-59 1.1182255638660834 1103 60-61 1.168810738089391 1103 62-63 1.036251881876531 1103 64-65 0.9199059186481193 1103 66-67 1.0507653900132752 1103 68-69 0.9382181801103613 1103 70-71 0.9410514519839523 1103 72-73 0.7087439909272177 1103 74-75 0.6802194437941012 1103 76-77 0.824074320180916 1103 78-79 0.9909303156349125 1103 80-81 0.7928642730966438 1103 82-83 0.8805903331335365 1103 84-85 1.0108433295109585 1103 86-87 1.0022420885530323 1103 88-89 1.1643106470674418 1103 90-91 1.222414266885682 1103 92-93 1.2091470939577427 1103 94-95 1.1518681839654832 1103 96-97 1.1861351441336616 1103 98-99 0.987534633522575 1103 100-101 1.3849482848998633 1104 1 -0.08892059225876281 1104 2 -0.0083513262260837 1104 3 0.08737371392174964 1104 4 -0.04415199607250031 1104 5 -0.06700211391552813 1104 6 0.011312985455539604 1104 7 -0.020048943729470636 1104 8 0.14047087835848515 1104 9 0.0874082111864638 1104 10-11 0.15300137632743116 1104 12-13 0.05352393026288382 1104 14-15 -0.04808093367207533 1104 16-17 0.08142613321322045 1104 18-19 -0.11652413574350362 1104 20-21 0.12957023289341407 1104 22-23 0.17942669524602195 1104 24-25 -0.10873727284831602 1104 26-27 -0.1102469796408414 1104 28-29 0.003367751694781873 1104 30-31 0.3232968802118279 1104 32-33 -0.01147113211127504 1104 34-35 0.002296216119233918 1104 36-37 -0.14253905281267265 1104 38-39 0.06582915369580888 1104 40-41 0.23495021353504342 1104 42-43 0.06338462896669483 1104 44-45 0.29181495917305256 1104 46-47 0.11027140729521534 1104 48-49 0.4526154316615987 1104 50-51 0.3180662864946342 1104 52-53 0.39774849779899313 1104 54-55 0.41888040154630346 1104 56-57 0.4879945833672821 1104 58-59 0.4357815019039464 1104 60-61 0.3753511855937006 1104 62-63 0.6300556684513978 1104 64-65 0.8106115985104267 1104 66-67 0.3881147876036337 1104 68-69 0.5346037222790372 1104 70-71 0.5709998168720816 1104 72-73 0.5710503592576828 1104 74-75 0.45904904792490697 1104 76-77 0.6665872289588854 1104 78-79 0.8239767872356012 1104 80-81 0.4813324314443186 1104 82-83 0.5070963572299227 1104 84-85 0.9170395429360916 1104 86-87 0.5805553415650806 1104 88-89 0.541246963762795 1104 90-91 0.8205209794502295 1104 92-93 0.7521763538544697 1104 94-95 0.773210696874262 1104 96-97 1.0191816157343467 1104 98-99 0.8395139794778252 1104 100-101 1.2274611936778328 1105 1 0.23297963076165118 1105 2 -0.04532626585785948 1105 3 0.1598314879807532 1105 4 -0.1480616934837471 1105 5 0.07448700071569192 1105 6 -0.014151428312551673 1105 7 -0.0022900284804094895 1105 8 0.11538187698727143 1105 9 0.24759477205895308 1105 10-11 0.14511453453193468 1105 12-13 0.026308122219511176 1105 14-15 0.3138913812543862 1105 16-17 0.23964655119356593 1105 18-19 0.28892920654034526 1105 20-21 0.17850587511903626 1105 22-23 0.1998150880037457 1105 24-25 0.214355998043537 1105 26-27 0.23990620770585025 1105 28-29 0.1814834359793167 1105 30-31 0.27732158743788204 1105 32-33 -0.04069762350065531 1105 34-35 -0.1690827245178852 1105 36-37 -0.21326133990135077 1105 38-39 0.28849483623054795 1105 40-41 0.3256441219916866 1105 42-43 0.3235215590477267 1105 44-45 0.4279624008011993 1105 46-47 0.29003667910333064 1105 48-49 -0.008990697104856338 1105 50-51 -0.3088899143319068 1105 52-53 -0.09886165551561277 1105 54-55 -0.3263370672197041 1105 56-57 -0.24663816468328292 1105 58-59 -0.04695748149200796 1105 60-61 0.009864947003208613 1105 62-63 -0.3422576672786377 1105 64-65 -0.028826428220206424 1105 66-67 -0.05396989652501105 1105 68-69 -0.05777476977208096 1105 70-71 -0.26959308019810635 1105 72-73 -0.2307048527707849 1105 74-75 -0.054505566625181245 1105 76-77 0.04333743018629477 1105 78-79 -0.07474458359231306 1105 80-81 -0.01146315014265653 1105 82-83 0.14243888922746706 1105 84-85 0.08594489765308211 1105 86-87 0.13918012323732043 1105 88-89 -0.1115888314911011 1105 90-91 -0.003187904143295839 1105 92-93 0.18678937684689245 1105 94-95 0.4575238671683266 1105 96-97 0.015549978013673638 1105 98-99 -0.1421146740324417 1105 100-101 0.04628176245942939 1106 1 0.02880746281869051 1106 2 0.049897997599835264 1106 3 0.004831299365562813 1106 4 0.019900130802348315 1106 5 -0.0025811648129945297 1106 6 -0.036117553024915594 1106 7 -0.01227908879954498 1106 8 0.13184043823062552 1106 9 0.08312987334530675 1106 10-11 0.20609948303999204 1106 12-13 0.1815667136412955 1106 14-15 0.19884554776387375 1106 16-17 0.33843375553926336 1106 18-19 0.15099492007159654 1106 20-21 0.4184809779143208 1106 22-23 0.08454103013660585 1106 24-25 0.002548687318878251 1106 26-27 0.19602299822982872 1106 28-29 0.1083837339913103 1106 30-31 0.2670514904896706 1106 32-33 0.27846002107288825 1106 34-35 0.36713516374647526 1106 36-37 0.37341865557188925 1106 38-39 0.29432681782169823 1106 40-41 0.473111020026316 1106 42-43 0.16773673125339172 1106 44-45 0.15654326013198983 1106 46-47 -0.1404416490116418 1106 48-49 -0.054416778812957034 1106 50-51 0.025455025122610664 1106 52-53 0.14960490301168505 1106 54-55 0.11044666660654201 1106 56-57 0.07364739871028547 1106 58-59 0.13982625233423818 1106 60-61 0.2818178686524675 1106 62-63 0.10993026889398294 1106 64-65 -0.4324176613863031 1106 66-67 -0.2727408799645943 1106 68-69 -0.13255880538262943 1106 70-71 -0.13753227066172258 1106 72-73 0.11555490806515678 1106 74-75 0.15844663828634964 1106 76-77 0.3712958593990159 1106 78-79 0.37050985829534966 1106 80-81 0.3953722642449087 1106 82-83 0.17709881604477573 1106 84-85 0.46754359998059414 1106 86-87 0.5504102714888575 1106 88-89 0.5473817068166404 1106 90-91 0.3678162831138607 1106 92-93 0.24342297298404958 1106 94-95 0.3688954768103301 1106 96-97 0.5246770669268734 1106 98-99 0.4035415182041575 1106 100-101 0.6133721845802214 1107 1 0.02776613512588355 1107 2 0.08524270495885844 1107 3 0.1623367616871647 1107 4 0.09704670697267659 1107 5 0.059619569694930874 1107 6 0.14637977392408885 1107 7 0.2032013630622913 1107 8 0.16806841461480104 1107 9 0.04446128518111436 1107 10-11 0.08421533074581333 1107 12-13 0.23622479555287867 1107 14-15 0.1516022768792098 1107 16-17 0.07748586841174188 1107 18-19 0.2805565397080585 1107 20-21 0.28482842805610886 1107 22-23 0.14407801121605246 1107 24-25 0.34289548663085867 1107 26-27 0.30823070591426216 1107 28-29 0.2563405932726113 1107 30-31 0.2099760407197877 1107 32-33 0.03395953295053289 1107 34-35 -0.04039586688900698 1107 36-37 0.23650265910496415 1107 38-39 0.2559557518554527 1107 40-41 0.33251783514416644 1107 42-43 0.22404745599887121 1107 44-45 0.2389173942959033 1107 46-47 0.33019895347080563 1107 48-49 0.06841804987934097 1107 50-51 0.06049058832283549 1107 52-53 0.13689512661820658 1107 54-55 0.23869268027397084 1107 56-57 0.1323592205688655 1107 58-59 -0.09012818957919677 1107 60-61 0.34588741446623317 1107 62-63 0.30157872782197614 1107 64-65 0.3515393838463652 1107 66-67 0.314149548188432 1107 68-69 0.22858433068729056 1107 70-71 0.021795515122292386 1107 72-73 0.2541119254051267 1107 74-75 0.2363672370497767 1107 76-77 0.31759542706207 1107 78-79 0.11170041196556468 1107 80-81 0.24069791158812848 1107 82-83 0.2289234638993598 1107 84-85 0.23848869783750715 1107 86-87 0.026436490632551823 1107 88-89 0.11686476199771434 1107 90-91 0.13026107879979065 1107 92-93 -0.08086227642322896 1107 94-95 -0.2084347263415438 1107 96-97 -0.18275401484524423 1107 98-99 -0.12219880070244571 1107 100-101 0.1949118619545871 1108 1 0.06144393204052889 1108 2 0.28319814692785883 1108 3 0.28319532790381174 1108 4 0.2691534906828821 1108 5 0.19395110663216997 1108 6 0.12157284020555181 1108 7 0.03976172752698659 1108 8 0.05134433735124588 1108 9 0.05096627619913363 1108 10-11 -0.09439412903898159 1108 12-13 0.16989009177303416 1108 14-15 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2316 48-49 -0.6142920085256947 2316 50-51 -0.9287136565680711 2316 52-53 -0.6433006004219166 2316 54-55 -0.6232026224929825 2316 56-57 -0.804237309675436 2316 58-59 -0.8236808566704994 2316 60-61 -1.1304995701714091 2316 62-63 -1.101337745420743 2316 64-65 -1.6598042558345085 2316 66-67 -1.051114030172549 2316 68-69 -1.4448278836300403 2316 70-71 -1.2656684540450343 2316 72-73 -1.4150089762563454 2316 74-75 -1.5810252642187237 2316 76-77 -1.8453691952484377 2316 78-79 -1.956480179425899 2316 80-81 -1.7382044839899287 2316 82-83 -1.8896436155250065 2316 84-85 -2.0231518251088723 2316 86-87 -2.01127847984106 2316 88-89 -2.271329000214987 2316 90-91 -2.4687926732649004 2316 92-93 -2.4259647821179904 2316 94-95 -2.8679069218742583 2316 96-97 -3.226309488832868 2316 98-99 -2.7964584817958418 2316 100-101 -3.478899795301782 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 884.0 3 247.0 4 204.0 5 222.0 6 252.0 7 296.0 8 357.0 9 445.0 10 674.0 11 968.0 12 1383.0 13 1957.0 14 2370.0 15 3253.0 16 4557.0 17 6546.0 18 5822.0 19 2729.0 20 2383.0 21 2320.0 22 2610.0 23 2863.0 24 3076.0 25 3454.0 26 3895.0 27 4660.0 28 5371.0 29 6384.0 30 7719.0 31 9697.0 32 12438.0 33 16446.0 34 23211.0 35 34610.0 36 52453.0 37 89780.0 38 111736.0 39 23076.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.296938682789204 23.5069623745875 17.079370848671687 21.116728093951608 2 13.503924618950746 23.75336285107722 41.46511720671728 21.277595323254754 3 15.085116095606582 31.44464683800685 32.45247813927927 21.017758927107295 4 9.89460618620682 20.134318929881527 43.35132379506389 26.61975108884776 5 9.60964889910922 40.00039915467909 37.529409938508 12.860542007703685 6 23.391391329227208 43.06198447005906 19.674363620636502 13.872260580077228 7 22.060531635693007 35.15613916867641 27.147246063271385 15.636083132359198 8 21.7465726848023 35.391311481589064 24.10528393003244 18.756831903576206 9 24.46206165728905 17.51974177186005 24.858670639407283 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17.76196258120552 29.120986253777776 34.824427683881204 18.292623481135504 36-37 17.61061054739993 28.751929044401535 34.497684038867824 19.139776369330715 38-39 17.00712225935925 30.07866723240013 34.611145023114936 18.30306548512568 40-41 16.79586105279484 29.59092444981139 35.49590370522188 18.117310792171896 42-43 17.16880550188773 29.523570023219953 33.47605774855097 19.83156672634135 44-45 17.109015134898005 29.567001446799644 33.80847297276339 19.51551044553896 46-47 18.103154135205077 29.58552678223262 33.47025771916764 18.84106136339466 48-49 17.79589386028756 29.86860485855165 32.827958883213306 19.507542397947482 50-51 17.509898324762542 29.870341362036072 32.8920168653717 19.727743447829678 52-53 17.59979260995716 30.728512918117488 32.20372969721275 19.467964774712595 54-55 18.125384312834804 30.005705833799585 32.409689946099256 19.459219907266352 56-57 18.273067898047678 30.26839704060854 31.372297029308076 20.0862380320357 58-59 17.887525383395907 29.6082565138253 32.58679653511799 19.917421567660806 60-61 18.490544299604622 30.79807640112864 31.76796283966797 18.943416459598776 62-63 17.868245481477214 30.589795461646013 32.860080344065814 18.681878712810956 64-65 18.084038640492757 29.844463154163158 32.4623343820623 19.609163823281783 66-67 17.892443905719727 30.272131078519248 31.573897310155857 20.26152770560516 68-69 17.627326012440864 29.970554630192975 32.745977443317386 19.656141914048778 70-71 18.909989271242498 29.308460644966793 32.24368466319682 19.537865420593896 72-73 17.922559317940618 29.421897952536785 32.25524494891866 20.40029778060394 74-75 17.66283002793002 30.712941205145505 31.508187894479693 20.116040872444778 76-77 17.44316167346215 30.074898067718493 32.61411983690835 19.86782042191101 78-79 17.51085591959251 29.14272861142844 32.694738964856626 20.651676504122424 80-81 18.02470340176174 30.650443095929102 31.10894816542544 20.21590533688372 82-83 18.526106998803243 29.269868356899075 31.92932494127033 20.27469970302735 84-85 17.6237204721138 29.05662551422092 31.74370332126052 21.575950692404763 86-87 17.590801204699126 29.956807237059348 32.238887670006406 20.213503888235124 88-89 18.090278508900738 28.691259734401964 32.101500430546764 21.116961326150534 90-91 17.47685788028933 28.375735156406705 32.71425230977915 21.433154653524817 92-93 18.235567918610705 29.506389015105338 32.073964070794496 20.184078995489454 94-95 17.423294803003394 28.2370850640221 32.809759588911334 21.52986054406318 96-97 17.026879998936366 28.99430180806522 32.68887517491776 21.289943018080653 98-99 17.103233527625715 29.514970856142376 31.953746592495737 21.428049023736175 100-101 16.732221052048114 29.19172994845075 32.55396042347985 21.522088576021282 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1517.0 1 1002.0 2 513.0 3 517.0 4 439.0 5 417.5 6 621.0 7 1263.0 8 2059.5 9 2328.0 10 1835.0 11 1307.5 12 1253.5 13 1240.5 14 1186.5 15 1133.5 16 1144.5 17 1206.5 18 1235.5 19 1316.0 20 1410.0 21 1479.5 22 1582.0 23 1781.5 24 1922.5 25 2089.5 26 2596.5 27 3107.0 28 3147.0 29 3876.0 30 5965.0 31 7824.5 32 11462.5 33 16574.0 34 19222.0 35 22179.0 36 29355.5 37 33500.5 38 31754.5 39 29637.0 40 26437.0 41 22734.0 42 18356.5 43 16584.5 44 16673.5 45 15748.0 46 13378.0 47 11771.5 48 14131.0 49 12409.0 50 7110.0 51 3932.5 52 2658.5 53 2237.5 54 1845.0 55 1634.5 56 1496.5 57 1382.0 58 1360.0 59 1297.5 60 1258.0 61 1160.5 62 880.5 63 555.0 64 279.0 65 181.5 66 138.5 67 101.5 68 71.0 69 50.5 70 46.5 71 42.0 72 36.5 73 36.0 74 29.5 75 20.0 76 13.0 77 11.0 78 10.0 79 4.5 80 1.5 81 0.0 82 1.0 83 1.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030796499390716738 2 0.021269524759056167 3 0.018610834164174143 4 0.04098814667109782 5 0.08795834718068019 6 0.10612606624570732 7 0.04741331560872936 8 0.07555112440456409 9 0.06114988368228647 10-11 0.06203611388058048 12-13 0.06148222000664673 14-15 0.05328459067242716 16-17 0.04397917359034009 18-19 0.057715741663897195 20-21 0.05328459067242716 22-23 0.02946715409327573 24-25 0.007311399135925557 26-27 0.0017724603965880137 28-29 0.005428159964550792 30-31 0.004320372216683284 32-33 0.005538938739337543 34-35 0.005760496288911045 36-37 0.005649717514124294 38-39 0.004098814667109782 40-41 0.006092832613271298 42-43 0.003101805694029024 44-45 0.0019940179461615153 46-47 0.0013293452974410104 48-49 3.323363243602526E-4 50-51 0.002437133045308519 52-53 0.005871275063697795 54-55 0.012739559100476347 56-57 0.009083859532513571 58-59 0.004763487315830287 60-61 0.0018832391713747645 62-63 0.009083859532513571 64-65 0.0022155754957350173 66-67 0.017392267641519885 68-69 0.03888334995014955 70-71 0.05007200620361139 72-73 0.002437133045308519 74-75 0.009637753406447324 76-77 0.01550902847014512 78-79 0.021823418632989918 80-81 0.07067685831394704 82-83 0.028802481444555224 84-85 0.013404231749196855 86-87 0.025922233300099705 88-89 0.039769580148443555 90-91 0.037775562202282044 92-93 0.04098814667109782 94-95 0.013404231749196855 96-97 0.014733577046637865 98-99 0.03101805694029024 100-101 0.07200620361138806 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 451350.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.29708875926208 #Duplication Level Percentage of deduplicated Percentage of total 1 76.00894557493359 16.94778205982215 2 9.33731077271031 4.163896941438716 3 3.3097947884806667 2.213963645210895 4 1.7228417659825954 1.5365742309711106 5 1.2028883957836831 1.341045466413758 6 0.8679194488215205 1.1611246191757958 7 0.6869362451527494 1.0721674901087548 8 0.5119524854557231 0.9132040006984871 9 0.4289601880084122 0.8608107047591984 >10 4.402591393475486 21.356622175494945 >50 0.8529208400260254 13.346913452579102 >100 0.6229421844504265 25.223916266339963 >500 0.03499675193541722 4.9606418494927755 >1k 0.007999257585238222 3.7143502567438045 >5k 9.999071981547777E-4 1.186986840750541 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5324 1.179572393929323 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4016 0.889775119087183 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2728 0.6044089952365127 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2027 0.44909715298548797 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2019 0.44732469258889995 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1786 0.39570178353827407 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1783 0.3950371108895535 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1288 0.28536612385067023 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 1013 0.22443779771795727 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 958 0.21225213249141464 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 876 0.19408441342638752 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 853 0.18898858978619698 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 832 0.18433588124515343 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 788 0.17458734906391934 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 760 0.16838373767586132 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 726 0.16085078099036224 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 711 0.1575274177467597 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 697 0.1544256120527307 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 696 0.1542040545031572 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 684 0.15154536390827517 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 683 0.15132380635870168 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 682 0.15110224880912818 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 645 0.1429046194749086 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 645 0.1429046194749086 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 618 0.13692256563642408 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 618 0.13692256563642408 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 598 0.132491414644954 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 584 0.129389608950925 No Hit TCCTTATTGTTTGCCTACTACGACAACTAAAATTTCACTTCACATCAAAA 575 0.1273955910047635 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 571 0.1265093608064695 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 569 0.12606624570732247 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 568 0.12584468815774896 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 565 0.12518001550902846 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 556 0.12318599756286695 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 547 0.12119197961670544 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 535 0.11853328902182343 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 534 0.11831173147224992 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 530 0.11742550127395592 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 513 0.11365902293120637 No Hit GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT 511 0.11321590783205937 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 508 0.11255123518333887 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 507 0.11232967763376536 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 505 0.11188656253461836 No Hit ATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTCTGAGC 502 0.11122188988589787 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 488 0.10812008419186885 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 479 0.10612606624570732 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 463 0.1025811454525313 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 461 0.1021380303533843 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 452 0.10014401240722277 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1077877478675086E-4 0.0 14-15 0.0 0.0 0.0 2.2155754957350172E-4 0.0 16-17 0.0 0.0 0.0 2.2155754957350172E-4 0.0 18-19 0.0 0.0 0.0 2.2155754957350172E-4 0.0 20-21 0.0 0.0 0.0 5.538938739337543E-4 0.0 22-23 0.0 0.0 0.0 0.0014401240722277612 0.0 24-25 0.0 0.0 0.0 0.0033233632436025255 0.0 26-27 0.0 0.0 0.0 0.005095823640190539 0.0 28-29 0.0 0.0 0.0 0.011299435028248588 0.0 30-31 0.0 0.0 0.0 0.022709648831283926 0.0 32-33 0.0 0.0 0.0 0.03323363243602526 0.0 34-35 0.0 0.0 0.0 0.04076658912152431 0.0 36-37 0.0 0.0 0.0 0.05671873269081644 0.0 38-39 0.0 0.0 0.0 0.07887448764816661 0.0 40-41 0.0 0.0 0.0 0.10379971197518556 0.0 42-43 0.0 0.0 0.0 0.14600642516893764 0.0 44-45 0.0 0.0 0.0 0.17835382740666889 0.0 46-47 0.0 0.0 0.0 0.24127617148554337 0.0 48-49 0.0 0.0 0.0 0.31715963221446775 0.0 50-51 0.0 0.0 0.0 0.3536058491193087 0.0 52-53 0.0 0.0 0.0 0.4056718732690816 0.0 54-55 0.0 0.0 0.0 0.4965104685942173 0.0 56-57 0.0 0.0 0.0 0.6388611941951923 0.0 58-59 0.0 0.0 0.0 0.8116760828625236 0.0 60-61 0.0 0.0 0.0 1.0059820538384847 0.0 62-63 0.0 0.0 0.0 1.196964661570843 0.0 64-65 0.0 0.0 0.0 1.421513238063587 0.0 66-67 0.0 0.0 0.0 1.7103135039326465 0.0 68-69 0.0 0.0 0.0 2.0540600420959345 0.0 70-71 0.0 0.0 0.0 2.2288689487094273 0.0 72-73 0.0 0.0 0.0 2.4492079317602746 0.0 74-75 0.0 0.0 0.0 2.7072116982386176 0.0 76-77 0.0 0.0 0.0 2.9766256785199956 0.0 78-79 0.0 0.0 0.0 3.2755068128946494 0.0 80-81 0.0 0.0 0.0 3.7397806580259223 0.0 82-83 0.0 0.0 0.0 4.191868837930652 0.0 84-85 0.0 0.0 0.0 4.9337542926775235 0.0 86-87 0.0 0.0 0.0 5.9453860640301315 0.0 88-89 0.0 0.0 0.0 6.968428049185777 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCACC 15 6.5928017E-4 95.081436 4 GGTACTC 75 2.3628672E-9 50.7101 3 CGCAGAA 50 2.8210481E-5 47.509094 9 GGTATAT 60 8.241016E-5 39.617264 1 TACCTGG 60 8.241016E-5 39.617264 2 GTCACTA 50 0.0016079905 38.032574 2 TACTCCC 105 4.6118657E-8 36.2215 5 GTGGTAC 105 4.6118657E-8 36.2215 1 CTCCCGC 120 3.776222E-9 35.631817 7 TATAAGC 75 3.0600838E-4 31.693811 2 CAATTGG 30 0.003938244 31.68678 78-79 GCAATTG 30 0.003938244 31.68678 76-77 TGCGAAT 30 0.003941453 31.681509 18-19 ACTCCCG 145 2.3836037E-8 29.494942 6 GGATATG 115 3.832067E-6 28.93783 1 CCGCTGT 75 3.3287506E-8 28.505455 10-11 TAGAGGC 85 6.369097E-4 27.96513 5 GAGGCAC 85 6.393935E-4 27.946526 7 GTCTAGA 70 0.008351277 27.166124 1 ATATGAG 140 5.7497346E-7 27.166124 3 >>END_MODULE