##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937052_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 451350 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.14068904397917 33.0 31.0 34.0 31.0 34.0 2 32.28462612163509 33.0 31.0 34.0 31.0 34.0 3 32.38606624570732 34.0 31.0 34.0 31.0 34.0 4 36.063742107012295 37.0 35.0 37.0 35.0 37.0 5 35.9150636977955 37.0 35.0 37.0 35.0 37.0 6 36.01359920239282 37.0 35.0 37.0 35.0 37.0 7 35.966746427384514 37.0 35.0 37.0 35.0 37.0 8 35.92870056497175 37.0 35.0 37.0 35.0 37.0 9 37.61763819652155 39.0 37.0 39.0 35.0 39.0 10-11 37.53408995236513 39.0 37.0 39.0 35.0 39.0 12-13 37.4746261216351 39.0 37.0 39.0 35.0 39.0 14-15 38.588422510247035 40.0 38.0 41.0 34.0 41.0 16-17 38.59125623130608 40.0 38.0 41.0 34.5 41.0 18-19 38.76016173701119 40.0 38.0 41.0 35.0 41.0 20-21 38.79516339869281 40.0 38.0 41.0 35.0 41.0 22-23 38.6704120970422 40.0 38.0 41.0 34.0 41.0 24-25 38.572643181566406 40.0 38.0 41.0 34.0 41.0 26-27 38.353714412318595 40.0 38.0 41.0 34.0 41.0 28-29 38.21612938960895 40.0 38.0 41.0 34.0 41.0 30-31 38.149593441896535 40.0 37.0 41.0 34.0 41.0 32-33 37.94055500166168 40.0 37.0 41.0 33.5 41.0 34-35 37.78741220782098 40.0 37.0 41.0 33.0 41.0 36-37 37.621138805804804 40.0 36.0 41.0 33.0 41.0 38-39 37.44391935305195 40.0 36.0 41.0 33.0 41.0 40-41 37.124887559543595 40.0 36.0 41.0 32.5 41.0 42-43 36.89875373878365 39.5 36.0 41.0 31.0 41.0 44-45 36.63419962335217 39.0 36.0 41.0 31.0 41.0 46-47 36.17839259997784 39.0 35.0 41.0 30.0 41.0 48-49 35.739509250027695 39.0 35.0 40.5 28.5 41.0 50-51 35.438738229755174 39.0 35.0 40.0 27.0 41.0 52-53 35.25606070676858 39.0 35.0 40.0 27.0 41.0 54-55 34.92342195635316 38.5 35.0 40.0 26.0 41.0 56-57 34.747826520438686 38.0 34.5 40.0 25.5 41.0 58-59 34.470828625235406 38.0 34.0 40.0 24.0 41.0 60-61 34.10548687271519 38.0 34.0 40.0 23.0 41.0 62-63 33.76196964661571 37.5 33.5 40.0 22.0 41.0 64-65 33.475288578708316 37.0 33.0 39.5 22.0 41.0 66-67 33.163046416306635 37.0 33.0 39.0 20.5 40.0 68-69 32.706099479339755 36.0 32.5 39.0 19.0 40.0 70-71 32.25955245374986 36.0 32.0 38.5 17.5 40.0 72-73 31.812313060817548 35.0 31.0 38.0 13.5 40.0 74-75 31.289702005095826 35.0 31.0 37.0 8.0 39.0 76-77 29.787392267641522 33.5 29.5 36.0 7.0 38.5 78-79 30.436901517669213 34.0 30.0 36.0 4.5 39.0 80-81 30.231364794505375 34.0 30.0 36.0 2.0 37.5 82-83 29.844079982275396 34.0 30.0 35.5 2.0 37.0 84-85 29.33579372992135 34.0 29.0 35.0 2.0 37.0 86-87 28.97736014179683 34.0 29.0 35.0 2.0 36.0 88-89 28.574650492965546 34.0 29.0 35.0 2.0 36.0 90-91 28.104895314057828 34.0 29.0 35.0 2.0 35.5 92-93 27.650704553007643 33.0 27.5 35.0 2.0 35.0 94-95 27.094801152099258 33.0 26.5 35.0 2.0 35.0 96-97 26.375649717514122 32.5 25.0 34.0 2.0 35.0 98-99 25.651452309737454 32.0 24.0 34.0 2.0 35.0 100-101 22.549672094826633 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14593140651685133 1101 2 0.33849048443377683 1101 3 0.2531988252388686 1101 4 0.15126513630247018 1101 5 0.010968644540497507 1101 6 0.05113582410632489 1101 7 0.025893380210078476 1101 8 0.14803474952132234 1101 9 0.3085309719195166 1101 10-11 0.19834085013878422 1101 12-13 0.2946270115672789 1101 14-15 0.324737777241765 1101 16-17 0.3157222244763176 1101 18-19 0.31638567114270444 1101 20-21 0.2082759515260264 1101 22-23 0.3942053584602263 1101 24-25 0.26913378359611073 1101 26-27 0.28825442259054057 1101 28-29 0.2048477133696025 1101 30-31 0.18023148125198674 1101 32-33 0.17630096237001425 1101 34-35 0.20360103784111772 1101 36-37 0.31410295739490834 1101 38-39 0.1393900590613555 1101 40-41 0.24379150203026256 1101 42-43 0.03283808491377016 1101 44-45 0.16353379732579043 1101 46-47 0.15525915082800879 1101 48-49 0.10020910763041968 1101 50-51 0.18896018029388983 1101 52-53 0.2582585834618243 1101 54-55 -0.16492559715786825 1101 56-57 -0.04190447084281601 1101 58-59 0.08250677416811669 1101 60-61 0.45860855688074054 1101 62-63 0.46584941020468307 1101 64-65 0.43316674259015286 1101 66-67 0.5642009925233893 1101 68-69 0.2527681952601739 1101 70-71 0.4285554399600997 1101 72-73 0.532600580088932 1101 74-75 0.38668132163926217 1101 76-77 0.4714568501205143 1101 78-79 0.5340626517466127 1101 80-81 0.30431245086269954 1101 82-83 0.4473525485726775 1101 84-85 0.37916544791113793 1101 86-87 0.15746679439346067 1101 88-89 0.21141032873996934 1101 90-91 0.21723153405509876 1101 92-93 0.3516389780302447 1101 94-95 0.05327937940655758 1101 96-97 -0.042360208500770824 1101 98-99 0.048396683618236125 1101 100-101 0.5521520068853221 1102 1 -0.206864646114731 1102 2 -0.10924343369487843 1102 3 0.02741300652541412 1102 4 0.04234700764749988 1102 5 -0.08387784668757803 1102 6 0.07964459603614671 1102 7 0.02571063167206944 1102 8 0.18166048051546824 1102 9 0.2851391590540189 1102 10-11 0.3052487448756267 1102 12-13 0.026626280573132988 1102 14-15 0.1492534936160439 1102 16-17 0.13100913968099093 1102 18-19 0.11152456003159017 1102 20-21 0.18479276439152414 1102 22-23 -0.046538428089476724 1102 24-25 0.005519017514238556 1102 26-27 0.18116377931568195 1102 28-29 -0.040081014700568574 1102 30-31 0.29024610113502547 1102 32-33 -0.15758061657735567 1102 34-35 -0.026753494322626636 1102 36-37 0.20774330827209297 1102 38-39 0.3012595766052115 1102 40-41 0.3890309026150618 1102 42-43 0.5408333334517863 1102 44-45 0.6698843090216968 1102 46-47 0.19244847831339484 1102 48-49 0.28615574505732155 1102 50-51 0.13527779725295375 1102 52-53 0.3322717413565641 1102 54-55 0.556428569508796 1102 56-57 0.06550598237355842 1102 58-59 0.006620443758762917 1102 60-61 0.27768750424916533 1102 62-63 0.2205551850584797 1102 64-65 0.27828735662524906 1102 66-67 0.07105406269882764 1102 68-69 0.4849958999385322 1102 70-71 0.35952217972617717 1102 72-73 0.39732097482577444 1102 74-75 0.16477890935855655 1102 76-77 0.31461291737197783 1102 78-79 -0.007923824861574502 1102 80-81 0.3053632549562657 1102 82-83 0.3742074462334948 1102 84-85 0.49023087188774994 1102 86-87 0.47074347568000974 1102 88-89 0.5997966591493267 1102 90-91 0.6377816071545155 1102 92-93 0.63736631721153 1102 94-95 0.19376731800304725 1102 96-97 0.28023664822437766 1102 98-99 0.27326875964162767 1102 100-101 0.07248278173912226 1103 1 0.2105998547458512 1103 2 0.2890521295103241 1103 3 0.24112798548888748 1103 4 0.22259179632602155 1103 5 -0.014959315419645236 1103 6 0.15130624262249626 1103 7 0.2669733013985933 1103 8 0.23091703052503476 1103 9 0.253841348765242 1103 10-11 0.17546173971211942 1103 12-13 0.23455023830883448 1103 14-15 0.3655547103878902 1103 16-17 0.2855125700692085 1103 18-19 0.24998614446456457 1103 20-21 0.25366049722763506 1103 22-23 0.3298525524995455 1103 24-25 0.15076368666704099 1103 26-27 0.2933386693520248 1103 28-29 0.21871757250539048 1103 30-31 0.3844293818770339 1103 32-33 0.4793600030439862 1103 34-35 0.23340021575578618 1103 36-37 0.34975779478959623 1103 38-39 0.21328715118927022 1103 40-41 0.3501686081992972 1103 42-43 0.20427429969772248 1103 44-45 0.29580913929517294 1103 46-47 0.45029833010149645 1103 48-49 0.5610170952198743 1103 50-51 0.44335086332677776 1103 52-53 0.2909098652808382 1103 54-55 0.6158134303808609 1103 56-57 0.29181150207138984 1103 58-59 0.2939856785072763 1103 60-61 0.38668599822506877 1103 62-63 0.49143483460906623 1103 64-65 0.6175909997692557 1103 66-67 0.7376938360780585 1103 68-69 0.566833667676157 1103 70-71 0.743873573396094 1103 72-73 0.9932358844643261 1103 74-75 0.7158268511256267 1103 76-77 0.8866764691983313 1103 78-79 0.6946169931724704 1103 80-81 0.5749042164211708 1103 82-83 0.6892279448948067 1103 84-85 0.5452739609105066 1103 86-87 0.49447082555570177 1103 88-89 0.7555790147622545 1103 90-91 0.7981746674911001 1103 92-93 0.3710361154521138 1103 94-95 0.7354743246008688 1103 96-97 0.7964159487598508 1103 98-99 0.8931261654553602 1103 100-101 1.8649308478129427 1104 1 0.031598133575457155 1104 2 0.06702114844319595 1104 3 0.034587537984585026 1104 4 -0.06484365978413109 1104 5 -0.2369902964867734 1104 6 -0.14645623586287826 1104 7 0.01051891241408498 1104 8 0.09666573964723568 1104 9 -0.07145985605403382 1104 10-11 -0.0319392929901241 1104 12-13 0.08136607307647381 1104 14-15 0.06004696512110996 1104 16-17 0.04196695905372394 1104 18-19 0.03828218577265119 1104 20-21 0.19083777777840538 1104 22-23 -0.01266035627842399 1104 24-25 0.08019570043640556 1104 26-27 0.09024056263946534 1104 28-29 0.04229760692879836 1104 30-31 0.08408514779786458 1104 32-33 0.024971018534522216 1104 34-35 0.13701467358711028 1104 36-37 0.13202973971213794 1104 38-39 0.029121918831265248 1104 40-41 -0.07065755359072057 1104 42-43 -0.10897871235047063 1104 44-45 -0.20677261629862898 1104 46-47 -0.25452094700693095 1104 48-49 0.11437337749181609 1104 50-51 -0.10053898176789033 1104 52-53 0.0034744091706784275 1104 54-55 -0.12084749905115899 1104 56-57 0.15928138158947291 1104 58-59 -0.22580778104521215 1104 60-61 -0.29317630814326634 1104 62-63 -0.18240337537372397 1104 64-65 0.020344872402638714 1104 66-67 -0.23907036357770295 1104 68-69 -0.07946581597273195 1104 70-71 -0.08917289820459473 1104 72-73 -0.10401024290228023 1104 74-75 -0.18778760670569739 1104 76-77 0.17239075491261602 1104 78-79 0.12835193293150482 1104 80-81 -0.06278941524836412 1104 82-83 0.26582002751098344 1104 84-85 0.10723609516179877 1104 86-87 -0.10019354621709908 1104 88-89 0.15144820581216578 1104 90-91 0.38939669847216507 1104 92-93 0.14814454918705167 1104 94-95 0.14080995282806086 1104 96-97 -0.13990074659298912 1104 98-99 -0.3168566228406817 1104 100-101 0.5249996946287787 1105 1 0.14835138900541978 1105 2 0.1738100680508552 1105 3 -0.015431391708304432 1105 4 0.2122997700217013 1105 5 0.03388247370675401 1105 6 0.06402922326966376 1105 7 0.21015558956896996 1105 8 0.15702759902029584 1105 9 0.31339829850770684 1105 10-11 0.13767711825825302 1105 12-13 0.11881345966534695 1105 14-15 0.1682977660539109 1105 16-17 -0.01938910687887585 1105 18-19 0.2083392293682138 1105 20-21 0.13934901310789627 1105 22-23 0.0019234609021054894 1105 24-25 0.16686824220103347 1105 26-27 0.1068287002440087 1105 28-29 0.16041093693220176 1105 30-31 0.1034745618045676 1105 32-33 0.053255797470704636 1105 34-35 0.08684144303617813 1105 36-37 -0.09738223085297903 1105 38-39 0.018050434584267805 1105 40-41 0.22831547080154735 1105 42-43 0.3731542027267878 1105 44-45 0.3258246330101926 1105 46-47 -0.06295881784598123 1105 48-49 -0.19972431244935507 1105 50-51 0.07070633750762312 1105 52-53 0.03507904265046591 1105 54-55 0.28837269304546 1105 56-57 0.26719812911826324 1105 58-59 0.1901427917768359 1105 60-61 0.1606877353027656 1105 62-63 0.31174273640372974 1105 64-65 0.2164612629715137 1105 66-67 0.4357047866667969 1105 68-69 0.4326221450607406 1105 70-71 0.33797482657357847 1105 72-73 0.027476358374755705 1105 74-75 -0.14966097819535307 1105 76-77 0.08248266732062959 1105 78-79 0.24118244696015623 1105 80-81 0.1653945113744193 1105 82-83 0.19641009308089608 1105 84-85 -0.06496366241087514 1105 86-87 0.009562085764216022 1105 88-89 0.059931889689480045 1105 90-91 0.10669366558540361 1105 92-93 -0.010203127232912834 1105 94-95 0.21941908171222835 1105 96-97 0.22422700815454633 1105 98-99 0.09034811371844143 1105 100-101 0.13091848465553468 1106 1 -0.10361209509432356 1106 2 -0.0681153157810499 1106 3 -0.11645745832719001 1106 4 0.04597512556132699 1106 5 -0.16534043172158874 1106 6 0.12917690896131973 1106 7 -0.004873268554689503 1106 8 0.05833281611637631 1106 9 0.24221115451886988 1106 10-11 0.1668558877327726 1106 12-13 0.17820649599263305 1106 14-15 0.3630467192396196 1106 16-17 0.12002203650639132 1106 18-19 -0.09821892069403049 1106 20-21 0.06273352402871524 1106 22-23 0.03703229186517376 1106 24-25 0.06458222613102294 1106 26-27 0.23935538929301003 1106 28-29 0.11292706353071935 1106 30-31 -0.06667708480601675 1106 32-33 -0.2481418410671523 1106 34-35 -0.29791279137477744 1106 36-37 -0.40956111349660773 1106 38-39 -0.39358523631995723 1106 40-41 -0.3053391370674774 1106 42-43 -0.011203939790846107 1106 44-45 0.03296183169743472 1106 46-47 -0.17192935615372562 1106 48-49 -0.3861798558497114 1106 50-51 -0.22989723109171933 1106 52-53 -0.12601907496996745 1106 54-55 0.1375616477400925 1106 56-57 0.03057471820121549 1106 58-59 0.07907296643450223 1106 60-61 -0.11076912840389497 1106 62-63 -0.3093519861433336 1106 64-65 -0.1395272692250913 1106 66-67 0.10296307630427037 1106 68-69 -0.1156595705830128 1106 70-71 -0.07476424317631825 1106 72-73 -0.0604277496640222 1106 74-75 -0.08100127771627186 1106 76-77 -0.31670440122212895 1106 78-79 -0.2961501570611169 1106 80-81 -0.2025484513929392 1106 82-83 0.06836490088202041 1106 84-85 -0.25049687754535555 1106 86-87 0.07684112307230251 1106 88-89 0.1690533201470572 1106 90-91 -0.009238659285394135 1106 92-93 0.29081728093035153 1106 94-95 0.23399903569012181 1106 96-97 0.36435300309966223 1106 98-99 0.5119238049931063 1106 100-101 1.1620909166597606 1107 1 0.14169030832718477 1107 2 0.18727959844888176 1107 3 0.1280639493247051 1107 4 0.09157505118078291 1107 5 -0.14661512838841162 1107 6 -0.15955908657584672 1107 7 0.07637818419041764 1107 8 0.014155418505851003 1107 9 0.051978250423289296 1107 10-11 0.14928348291662985 1107 12-13 0.07030588700188645 1107 14-15 0.338807957675769 1107 16-17 0.1676790491975737 1107 18-19 -0.04434183455205698 1107 20-21 0.1756060078741868 1107 22-23 -0.02334611918034568 1107 24-25 0.08317736350259963 1107 26-27 0.30130741383860027 1107 28-29 0.1910847502956301 1107 30-31 0.04482168326612879 1107 32-33 -0.23460225666847379 1107 34-35 0.027481193098289225 1107 36-37 0.09752595823412946 1107 38-39 -0.10929115422242575 1107 40-41 0.1759657766975664 1107 42-43 0.16091962489699085 1107 44-45 0.30872771891792894 1107 46-47 0.5366058118998254 1107 48-49 0.4717416726705821 1107 50-51 -0.025174066199333822 1107 52-53 0.15722903065041294 1107 54-55 0.2000017946416719 1107 56-57 0.1102711525836284 1107 58-59 -0.1409470233381782 1107 60-61 -0.027302529919403185 1107 62-63 0.12228299131005826 1107 64-65 0.49808139610892255 1107 66-67 0.35464128745206125 1107 68-69 0.08793858849839609 1107 70-71 0.17172360025262634 1107 72-73 0.11370408985994374 1107 74-75 0.07536523244132454 1107 76-77 -0.029597804597141675 1107 78-79 -0.028945006208065394 1107 80-81 -0.16737110027026247 1107 82-83 -0.01881607629244897 1107 84-85 0.049335766218298716 1107 86-87 0.17455856995994168 1107 88-89 -0.05865808332093536 1107 90-91 -0.1851370368596612 1107 92-93 0.06724561991970646 1107 94-95 0.16540622271071825 1107 96-97 -0.04382885734383635 1107 98-99 -0.0915558100875522 1107 100-101 0.23695261532555634 1108 1 0.2338111668933962 1108 2 0.15481483188777645 1108 3 0.24036185476389704 1108 4 0.11890527323015476 1108 5 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554.0 11 896.0 12 1369.0 13 1757.0 14 2284.0 15 3336.0 16 4548.0 17 6394.0 18 5545.0 19 3058.0 20 2561.0 21 2485.0 22 2789.0 23 2966.0 24 3461.0 25 4063.0 26 4623.0 27 5761.0 28 6783.0 29 8560.0 30 10392.0 31 12929.0 32 16276.0 33 20827.0 34 29090.0 35 41315.0 36 63071.0 37 103272.0 38 76308.0 39 3565.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.97793526169883 23.94124933796921 17.344652674825987 19.736162725505967 2 14.221809625516793 25.334668610499357 40.75401801737929 19.68950374660457 3 16.79424830229647 31.838615693095086 31.39616036513089 19.97097563947756 4 11.239614489863742 21.04663786418522 42.23064140910602 25.48310623684502 5 10.460790591425843 40.62166958764371 36.924399501033605 11.993140319896838 6 25.379860418743768 43.42572283150548 18.185665226542593 13.008751523208154 7 24.092167940622577 35.367231638418076 26.012850337875264 14.527750083084081 8 23.81832280934973 35.36147114212917 23.147889664340312 17.672316384180792 9 26.089066134928547 18.82640965990916 24.033233632436023 31.051290572726266 10-11 21.642849230087517 29.670876260108564 30.540600420959347 18.145674088844576 12-13 24.073140603448753 28.44396191193659 29.58039982495887 17.90249765965579 14-15 21.851648747306555 30.073394597049813 28.713406561753935 19.361550093889694 16-17 18.038440234851002 32.41940844134264 30.444555223219233 19.09759610058713 18-19 17.623723548739445 31.26294729798892 33.519848277053896 17.593480876217736 20-21 18.934877156600134 30.001196418719967 33.83505373914083 17.228872685539066 22-23 19.187871805381693 29.054603462982865 34.234344016218195 17.523180715417254 24-25 19.172942325970926 30.305396956222154 33.5090296379504 17.01263107985652 26-27 18.563683535357338 29.95686264952398 34.59030594949806 16.889147865620618 28-29 17.738973031895565 29.26788198906387 35.194794260747805 17.798350718292756 30-31 18.78621777580345 29.91395803030689 33.91540258713037 17.384421606759286 32-33 18.218919041673914 30.893243484262197 33.793435020975984 17.094402453087906 34-35 18.89808352719619 29.203279051733688 34.473690040988146 17.424947380081974 36-37 18.61495887837215 29.44273104328406 33.674204154702394 18.268105923641393 38-39 17.9089314775896 30.891087134502275 34.017253852315264 17.182727535592857 40-41 18.21611950880419 30.085577077528956 34.447626274655335 17.250677139011515 42-43 18.128281821203057 30.632214467707986 32.65702891326022 18.582474797828734 44-45 18.431363860440126 30.271021773688787 32.802441577720046 18.495172788151038 46-47 19.363661742159046 30.61717344488067 32.151909391423025 17.867255421537262 48-49 19.07535069829478 30.935228686417066 31.768507553459123 18.220913061829023 50-51 18.77860210682108 30.785421746408225 31.85600324364924 18.579972903121458 52-53 18.93330526922161 31.132442297786074 31.489816604722527 18.444435828269793 54-55 19.356042982164617 31.01639525866844 31.056497175141246 18.571064584025702 56-57 19.422331721129325 31.15981203015848 30.60602770804854 18.811828540663655 58-59 19.121323940759876 30.245519270543113 31.81071431340901 18.822442475288 60-61 19.271928444664034 31.96903463662891 30.495764861757024 18.263272056950036 62-63 18.870610391049077 31.359144787858646 32.0485211033566 17.72172371773568 64-65 18.77157416638972 31.065913370998118 31.431150991470037 18.73136147114213 66-67 19.01019164728038 31.208928769247812 30.35692921236291 19.423950371108894 68-69 18.32687859478717 30.933448248358253 31.664702181022182 19.074970975832393 70-71 19.54278928226824 30.60393866782764 31.17755688269851 18.675715167205613 72-73 18.87011295103978 30.661621018021652 30.927935552768833 19.54033047816974 74-75 18.822452850255246 31.921019746171297 30.13972100113443 19.116806402439025 76-77 19.059820538384844 30.6672205605406 31.233078542151322 19.03988035892323 78-79 18.777611854320188 30.36428815144036 30.812281486930203 20.04581850730925 80-81 19.107446915757603 32.4171983307281 29.261224213969903 19.214130539544396 82-83 19.708141593312494 30.454703566419777 30.280337388584 19.55681745168373 84-85 18.635796629096475 30.700613515962715 29.88170596876364 20.78188388617717 86-87 18.29594147283902 31.544092558702193 30.230133866257813 19.929832102200972 88-89 18.69900505504269 30.405367230073683 30.446357056841812 20.449270658041815 90-91 18.635721605228902 29.617304123854097 30.884094496911956 20.86287977400504 92-93 18.890993685609835 31.06746427384513 30.122078209815 19.919463830730034 94-95 18.269635537830954 29.63110667996012 30.931427938407 21.16782984380193 96-97 18.17787038975685 30.40187616803535 30.269494212305208 21.15075922990259 98-99 17.983207799876148 31.239607347446107 29.872679274295127 20.90450557838262 100-101 17.380197214710563 30.802917120412644 30.053474632507303 21.76341103236949 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1733.0 1 1161.5 2 569.5 3 512.5 4 482.5 5 515.5 6 660.0 7 1085.0 8 1584.5 9 1789.5 10 1630.5 11 1391.5 12 1319.0 13 1213.5 14 1114.0 15 1129.5 16 1147.5 17 1145.0 18 1177.5 19 1214.5 20 1296.5 21 1419.0 22 1512.5 23 1682.5 24 1846.0 25 2030.0 26 2404.0 27 2741.0 28 2915.0 29 3923.0 30 5944.5 31 7560.0 32 10863.0 33 15379.5 34 18269.5 35 21706.5 36 28544.5 37 32628.5 38 31819.0 39 30414.5 40 27413.5 41 23223.0 42 18383.5 43 16996.5 44 17689.0 45 16332.5 46 13396.5 47 12200.0 48 14468.5 49 12793.0 50 7690.0 51 4318.0 52 2783.0 53 2322.5 54 1933.5 55 1655.5 56 1483.0 57 1392.5 58 1418.0 59 1458.5 60 1435.0 61 1326.0 62 1011.5 63 604.0 64 314.5 65 200.0 66 137.0 67 95.5 68 58.0 69 42.5 70 36.5 71 24.0 72 21.0 73 19.0 74 18.0 75 16.5 76 12.0 77 9.0 78 4.0 79 3.0 80 2.0 81 0.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01927550681289465 2 0.0017724603965880137 3 0.0011077877478675086 4 0.0 5 0.003766478342749529 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006092832613271298 14-15 0.004985044865403789 16-17 0.0 18-19 2.2155754957350172E-4 20-21 6.646726487205052E-4 22-23 0.0011077877478675086 24-25 6.646726487205052E-4 26-27 2.2155754957350172E-4 28-29 4.4311509914700343E-4 30-31 1.1077877478675086E-4 32-33 1.1077877478675086E-4 34-35 0.0 36-37 0.0013293452974410104 38-39 0.006757505261991803 40-41 5.538938739337543E-4 42-43 0.0 44-45 5.538938739337543E-4 46-47 4.4311509914700343E-4 48-49 1.1077877478675086E-4 50-51 0.0014401240722277612 52-53 5.538938739337543E-4 54-55 0.0 56-57 2.2155754957350172E-4 58-59 1.1077877478675086E-4 60-61 0.001550902847014512 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.4311509914700343E-4 70-71 9.970089730807576E-4 72-73 8.862301982940069E-4 74-75 0.004874266090617038 76-77 0.0 78-79 8.862301982940069E-4 80-81 0.003434142018389277 82-83 2.2155754957350172E-4 84-85 0.003766478342749529 86-87 8.862301982940069E-4 88-89 0.004098814667109782 90-91 0.0027694693696687714 92-93 0.0 94-95 0.0 96-97 9.970089730807576E-4 98-99 0.0021047967209482663 100-101 0.0032125844688157745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 451350.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.263676046621754 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1665857157802 16.734845139067563 2 9.92441887603928 4.41908093614233 3 3.5458129162574576 2.3682849026844957 4 1.8798655633186094 1.6741087165170254 5 1.1579166107552836 1.2889740155428926 6 0.8349398592023519 1.1153298326215886 7 0.640953831802986 0.9988991930471798 8 0.5189626188807044 0.9243212501653146 9 0.4989640593861356 0.9997896759372235 >10 4.343687122191097 21.0708289997287 >50 0.8329400029431824 13.001495580958652 >100 0.611955920529685 25.59270116575781 >500 0.033997551140538054 5.1209445369814715 >1k 0.008999351772495367 4.690396054847742 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4257 0.9431704885343968 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3432 0.7603855101362579 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3026 0.6704331450094162 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2697 0.5975407111997342 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2390 0.5295225434806692 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1978 0.43824083305638634 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 1215 0.2691924227318046 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1048 0.2321923119530298 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1026 0.22731804586241275 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 991 0.2195635316273402 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 979 0.2169048410324582 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 958 0.21225213249141464 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 935 0.2071563088512241 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 911 0.20183892766146008 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 883 0.19563531627340203 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 854 0.18921014733577046 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 816 0.1807909604519774 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 739 0.16373102913481777 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 731 0.16195856873822975 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 724 0.16040766589121525 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 723 0.16018610834164174 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 705 0.15619807244931871 No Hit CGTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCG 685 0.15176692145784867 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 653 0.1446770798714966 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 646 0.1431261770244821 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 623 0.13803035338429157 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 622 0.13780879583471808 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 618 0.13692256563642408 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 604 0.13382075994239503 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 591 0.13094051179793953 No Hit TACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTA 581 0.1287249363022045 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 581 0.1287249363022045 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 568 0.12584468815774896 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 563 0.12473690040988147 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 558 0.12362911266201396 No Hit CTCCTATTGTACAAAAAGGACTACGATATGGTATAATTCTATTCATCGTC 537 0.11897640412097044 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 535 0.11853328902182343 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 534 0.11831173147224992 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 529 0.11720394372438239 No Hit GGCTAATTGTTTCAATAATAATAAGTATTGGAATTAGTGAAATTGGAGTT 513 0.11365902293120637 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 506 0.11210812008419187 No Hit GTATTAAGTAGTGGGACTTCTAGAGGGTTAAGTGGTGAAATTCCTGTTGG 504 0.11166500498504486 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 503 0.11144344743547135 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 493 0.10922787193973635 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 489 0.10834164174144234 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 486 0.10767696909272184 No Hit GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT 469 0.1039104907499723 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 468 0.10368893320039882 No Hit CTACCCAACTATCCATAAATCTAAGTATAGCCATTCCACTATGAGCTGGA 462 0.10235958790295778 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 457 0.10125180015509029 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1077877478675086E-4 0.0 14-15 0.0 0.0 0.0 2.2155754957350172E-4 0.0 16-17 0.0 0.0 0.0 2.2155754957350172E-4 0.0 18-19 0.0 0.0 0.0 2.2155754957350172E-4 0.0 20-21 0.0 0.0 0.0 5.538938739337543E-4 0.0 22-23 0.0 0.0 0.0 0.0016616816218012627 0.0 24-25 0.0 0.0 0.0 0.0035449207931760275 0.0 26-27 0.0 0.0 0.0 0.005760496288911045 0.0 28-29 0.0 0.0 0.0 0.011964107676969093 0.0 30-31 0.0 0.0 0.0 0.02304198515564418 0.0 32-33 0.0 0.0 0.0 0.03279051733687825 0.0 34-35 0.0 0.0 0.0 0.04021269524759056 0.0 36-37 0.0 0.0 0.0 0.05649717514124294 0.0 38-39 0.0 0.0 0.0 0.07887448764816661 0.0 40-41 0.0 0.0 0.0 0.10402126952475906 0.0 42-43 0.0 0.0 0.0 0.1474465492411654 0.0 44-45 0.0 0.0 0.0 0.18034784535283038 0.0 46-47 0.0 0.0 0.0 0.24338096820649163 0.0 48-49 0.0 0.0 0.0 0.3193752077102027 0.0 50-51 0.0 0.0 0.0 0.35626453971419075 0.0 52-53 0.0 0.0 0.0 0.4068904397917359 0.0 54-55 0.0 0.0 0.0 0.4970643624681511 0.0 56-57 0.0 0.0 0.0 0.6422953362135815 0.0 58-59 0.0 0.0 0.0 0.8163287914035671 0.0 60-61 0.0 0.0 0.0 1.0116317713526088 0.0 62-63 0.0 0.0 0.0 1.2040545031571952 0.0 64-65 0.0 0.0 0.0 1.429156973523873 0.0 66-67 0.0 0.0 0.0 1.7197296997895202 0.0 68-69 0.0 0.0 0.0 2.0646948044754625 0.0 70-71 0.0 0.0 0.0 2.240611498836823 0.0 72-73 0.0 0.0 0.0 2.459621136590229 0.0 74-75 0.0 0.0 0.0 2.7185111332668663 0.0 76-77 0.0 0.0 0.0 2.987703555998671 0.0 78-79 0.0 0.0 0.0 3.286363132823751 0.0 80-81 0.0 0.0 0.0 3.7521878808020386 0.0 82-83 0.0 0.0 0.0 4.2062700786529295 0.0 84-85 0.0 0.0 0.0 4.944942948930985 0.0 86-87 0.0 0.0 0.0 5.952475905616484 0.0 88-89 0.0 0.0 0.0 6.974631660573834 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCATACG 25 0.0050206026 56.99628 2 GTATGGT 55 4.9604114E-5 43.178997 3 ATACAGT 45 9.6167007E-4 42.22414 6 ATGTATT 50 0.001614415 38.001728 1 GCTTTGC 50 0.001614415 38.001728 1 GGTACTC 100 3.025525E-8 37.99752 3 TACAATC 95 8.6812724E-7 35.00159 7 TCCGGTG 55 0.0025787984 34.543198 2 GTGGTAC 115 1.03360435E-7 33.044983 1 GACCGTG 60 0.0039475528 31.668106 6 ACCCTTG 330 0.0 31.668106 7 CTGACCG 60 0.0039496943 31.664598 4 TGACCGT 60 0.0039496943 31.664598 5 GTCACTA 60 0.0039496943 31.664598 2 CCCTTGG 345 0.0 30.294588 8 CATATAG 95 3.487752E-5 29.99804 3 CGTGCAA 65 0.0058359024 29.235338 9 CTATATG 65 0.0058390633 29.232098 1 ACCGTGC 65 0.0058390633 29.232098 7 CGGTGTT 65 0.0058422256 29.22886 4 >>END_MODULE