FastQCFastQC Report
Thu 26 May 2016
SRR937051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042940
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98450.48190353118544843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74750.365894250442989No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46300.22663416448843332No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25370.12418377436439641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA115050.035.8944241
ATCAACG137950.029.7269943
TCAACGC138900.029.5236764
CAACGCA139750.029.2760985
TATCAAC141100.028.9981252
GGTATCA71750.028.6787241
AACGCAG142950.028.5867986
GTACATG96350.026.4366781
ACGCAGA159400.025.3981747
CGCAGAG160950.025.0354928
TACATGG99600.024.810632
ACATGGG97650.024.1363353
GCAGAGT166850.023.3243189
GAGTACT75350.022.98188812-13
AGAGTAC127500.022.13554610-11
CATGGGA61950.021.4005414
GGACCGT2457.496055E-921.3342726
GTACTTT81850.021.06949214-15
AGTACTT83100.020.75281312-13
CATGGGG38250.019.0073644
Produced by FastQC (version 0.11.5)