##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937051_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2042940 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.777214210892147 33.0 31.0 34.0 30.0 34.0 2 31.877870617835082 33.0 31.0 34.0 30.0 34.0 3 31.88519829265666 33.0 31.0 34.0 30.0 34.0 4 35.453310914662204 37.0 35.0 37.0 33.0 37.0 5 35.47873946371406 37.0 35.0 37.0 33.0 37.0 6 35.5361983220261 37.0 35.0 37.0 33.0 37.0 7 35.4757795138379 37.0 35.0 37.0 33.0 37.0 8 35.44808609161307 37.0 35.0 37.0 33.0 37.0 9 37.07440159769744 39.0 37.0 39.0 33.0 39.0 10-11 36.94393178458496 39.0 37.0 39.0 33.0 39.0 12-13 36.84544896081138 39.0 37.0 39.0 33.0 39.0 14-15 38.003916659324304 40.0 38.0 41.0 33.0 41.0 16-17 37.932842618970696 40.0 38.0 41.0 33.0 41.0 18-19 37.9137228699815 40.0 38.0 41.0 33.0 41.0 20-21 37.85928049771408 40.0 38.0 41.0 33.0 41.0 22-23 37.7155684944247 40.0 37.5 41.0 32.0 41.0 24-25 37.553356437291356 40.0 37.0 41.0 32.0 41.0 26-27 37.512629592645894 40.0 37.0 41.0 32.0 41.0 28-29 37.5933157606195 40.0 37.0 41.0 32.0 41.0 30-31 37.46532619655986 40.0 37.0 41.0 32.0 41.0 32-33 37.279319999608404 40.0 37.0 41.0 31.5 41.0 34-35 37.084631217754804 40.0 36.0 41.0 31.0 41.0 36-37 36.93529325384005 39.5 36.0 41.0 31.0 41.0 38-39 36.77691390838693 39.0 36.0 41.0 30.0 41.0 40-41 36.584413883912404 39.0 35.5 41.0 30.0 41.0 42-43 36.385694391416294 39.0 35.0 41.0 30.0 41.0 44-45 36.124749625539664 39.0 35.0 41.0 29.5 41.0 46-47 35.77909679187837 39.0 35.0 40.5 28.5 41.0 48-49 35.439140894984675 39.0 35.0 40.0 27.0 41.0 50-51 34.79628501081774 38.5 34.0 39.5 25.5 40.5 52-53 35.10009129000362 38.5 35.0 40.0 26.5 40.5 54-55 35.42003313851606 39.0 35.0 40.5 27.0 41.0 56-57 35.30724911157449 39.0 35.0 41.0 26.5 41.0 58-59 35.083594721332986 39.0 35.0 41.0 26.0 41.0 60-61 34.81284619225234 38.0 34.0 40.0 26.0 41.0 62-63 34.51920418612392 38.0 34.0 40.0 25.0 41.0 64-65 34.22226692903365 37.5 34.0 40.0 24.5 41.0 66-67 33.85394994468756 37.0 34.0 40.0 23.0 41.0 68-69 33.41973846515316 36.5 33.0 39.0 22.0 41.0 70-71 33.06578631775774 36.0 33.0 39.0 22.0 41.0 72-73 32.68245934780268 35.5 32.5 39.0 21.0 40.0 74-75 32.2488320753424 35.0 32.0 37.5 19.5 40.0 76-77 31.813460992491216 35.0 32.0 37.0 18.5 39.0 78-79 31.37560403144488 35.0 31.0 37.0 17.0 39.0 80-81 30.93844386031895 35.0 31.0 36.0 12.0 38.5 82-83 30.51665149245695 35.0 31.0 36.0 7.5 37.0 84-85 30.1497677366932 34.0 30.5 35.0 7.0 37.0 86-87 29.82151433718073 34.0 30.0 35.0 2.0 36.5 88-89 29.444347117389643 34.0 30.0 35.0 2.0 36.0 90-91 29.09887270306519 34.0 29.0 35.0 2.0 36.0 92-93 28.745555914515357 34.0 29.0 35.0 2.0 35.5 94-95 28.356251284912922 34.0 29.0 35.0 2.0 35.0 96-97 27.92398895709125 34.0 29.0 35.0 2.0 35.0 98-99 27.341225146112954 34.0 27.5 35.0 2.0 35.0 100-101 25.149609631217754 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5111923869842023 1101 2 0.4168687058677385 1101 3 0.5079938544668394 1101 4 0.3514730329878972 1101 5 0.32009580207869703 1101 6 0.3545862628015968 1101 7 0.4252198142067698 1101 8 0.29679387572554816 1101 9 0.3702067027684848 1101 10-11 0.3965395128471769 1101 12-13 0.5942271919427426 1101 14-15 0.765287356147411 1101 16-17 0.7595652976424603 1101 18-19 0.7473545004356268 1101 20-21 0.658470362652217 1101 22-23 0.6044813546116004 1101 24-25 0.7500599527003331 1101 26-27 0.7866116062435111 1101 28-29 0.7291568414600746 1101 30-31 0.7088687413209342 1101 32-33 0.7018826566388441 1101 34-35 0.732083318318999 1101 36-37 0.7136215348022077 1101 38-39 0.8563094906058311 1101 40-41 0.8598302373431892 1101 42-43 0.8107412701600438 1101 44-45 0.8538996174928357 1101 46-47 0.8357625394634098 1101 48-49 0.8526004175890023 1101 50-51 0.7304764592442154 1101 52-53 0.8507715160139426 1101 54-55 0.8980385640318573 1101 56-57 0.9481133573793628 1101 58-59 0.9315281713933032 1101 60-61 0.9900497073304066 1101 62-63 1.0208751951662478 1101 64-65 0.9731418730325032 1101 66-67 0.9246110938719028 1101 68-69 0.9021469975424949 1101 70-71 0.8870348935651933 1101 72-73 0.9170734994871594 1101 74-75 0.8340251322084455 1101 76-77 0.8289511816626955 1101 78-79 0.8997010363591187 1101 80-81 1.011559890069698 1101 82-83 0.7383828928693816 1101 84-85 1.0801677702565833 1101 86-87 1.1392355167838204 1101 88-89 0.9709694669765945 1101 90-91 0.9749316565988231 1101 92-93 1.006644610700132 1101 94-95 1.21374350594672 1101 96-97 1.2628230280401915 1101 98-99 1.1179006060565655 1101 100-101 1.3859792894566283 1102 1 0.3074633849891022 1102 2 0.2651820120921293 1102 3 0.22483033274161102 1102 4 0.09288955116458908 1102 5 0.15575090937222313 1102 6 0.12394250166484255 1102 7 0.11061220847430064 1102 8 0.03823400000023014 1102 9 0.12967960144501944 1102 10-11 0.26902622489847516 1102 12-13 0.26017729931241007 1102 14-15 0.40345765510722487 1102 16-17 0.378283791116516 1102 18-19 0.3004053982288397 1102 20-21 0.2952943525396634 1102 22-23 0.18752227692470314 1102 24-25 0.39526981852632304 1102 26-27 0.4340092243120779 1102 28-29 0.5182302640396941 1102 30-31 0.27272590158350596 1102 32-33 0.36267635457216585 1102 34-35 0.2771568779662772 1102 36-37 0.21785959087811335 1102 38-39 0.30917725076787406 1102 40-41 0.3504430059577359 1102 42-43 0.21490965017253671 1102 44-45 0.2919355139915254 1102 46-47 0.2795221532371883 1102 48-49 0.2818548456360972 1102 50-51 0.33434938228833033 1102 52-53 0.35141268809478987 1102 54-55 0.2369219955970152 1102 56-57 0.38650220311731687 1102 58-59 0.27196732096458476 1102 60-61 0.428623975160626 1102 62-63 0.3600011603510538 1102 64-65 0.4655784030883936 1102 66-67 0.34577319923125316 1102 68-69 0.3680746410447213 1102 70-71 0.43215756912602643 1102 72-73 0.4215171570616789 1102 74-75 0.43939704247481615 1102 76-77 0.39675094103279207 1102 78-79 0.4003623878463003 1102 80-81 0.4138260754765568 1102 82-83 0.42418953608224896 1102 84-85 0.4996980089066163 1102 86-87 0.507548137669886 1102 88-89 0.44084542073909816 1102 90-91 0.3347350406471925 1102 92-93 0.5487674080988931 1102 94-95 0.5854432211505731 1102 96-97 0.40465912557145245 1102 98-99 0.5872385692310544 1102 100-101 0.9122475461082153 1103 1 0.3012614216558056 1103 2 0.37408070787321535 1103 3 0.4085222203891661 1103 4 0.35559582985627003 1103 5 0.21236893255036904 1103 6 0.1920057544904381 1103 7 0.27403966842405936 1103 8 0.284203725700479 1103 9 0.2856797252529617 1103 10-11 0.3281305884767818 1103 12-13 0.17886504916823753 1103 14-15 0.24451139101184793 1103 16-17 0.5174281153369336 1103 18-19 0.4189837572523132 1103 20-21 0.41284095156809286 1103 22-23 0.26597717010776734 1103 24-25 0.6121178801175162 1103 26-27 0.5509604048859558 1103 28-29 0.5474491010921483 1103 30-31 0.5701370123687965 1103 32-33 0.5651727950938579 1103 34-35 0.6528207165989173 1103 36-37 0.6743479415282678 1103 38-39 0.6697972841960151 1103 40-41 0.715064723825499 1103 42-43 0.7774166143692298 1103 44-45 0.6174346554811976 1103 46-47 0.7256744141982665 1103 48-49 0.8331858624576824 1103 50-51 0.8778693398026576 1103 52-53 0.8453123775975016 1103 54-55 0.8246140200849226 1103 56-57 0.9342485727752461 1103 58-59 0.9923924546282876 1103 60-61 0.9816016376015284 1103 62-63 0.8697403654389078 1103 64-65 0.8304406504631814 1103 66-67 1.0571634483784464 1103 68-69 1.0513476994592637 1103 70-71 0.8989414460739624 1103 72-73 0.9705907967219218 1103 74-75 0.9615223361114076 1103 76-77 0.7348557672618341 1103 78-79 0.867063834770164 1103 80-81 0.9209644178185457 1103 82-83 0.9418712649624048 1103 84-85 0.9765945510811385 1103 86-87 1.0048685917192195 1103 88-89 1.0693130205266712 1103 90-91 0.9883885967044996 1103 92-93 0.9441537703283451 1103 94-95 0.9589274698481454 1103 96-97 0.9640957342764516 1103 98-99 1.1486769568606867 1103 100-101 1.464002930939138 1104 1 0.37344334692044967 1104 2 0.4416738724464331 1104 3 0.3611334410991418 1104 4 0.2929490061200326 1104 5 0.3133969477923628 1104 6 0.2460208370659842 1104 7 0.3246510972611816 1104 8 0.3408702152376577 1104 9 0.4391353000713494 1104 10-11 0.48575562528650806 1104 12-13 0.45024762228396753 1104 14-15 0.4539802903956627 1104 16-17 0.46869038432134147 1104 18-19 0.5030139843987413 1104 20-21 0.4909425995365311 1104 22-23 0.5137500436129656 1104 24-25 0.42972405263720503 1104 26-27 0.5448895685912447 1104 28-29 0.4970618517580476 1104 30-31 0.6678106380100601 1104 32-33 0.5543875468801005 1104 34-35 0.6678537512476552 1104 36-37 0.6684716709009848 1104 38-39 0.5162069249110033 1104 40-41 0.5688958131496875 1104 42-43 0.5831634144144004 1104 44-45 0.4328283700414559 1104 46-47 0.3595989725369648 1104 48-49 0.3390037357082534 1104 50-51 0.4723131200115347 1104 52-53 0.5360138546359821 1104 54-55 0.4297586352379099 1104 56-57 0.5221164208955713 1104 58-59 0.6547092086419326 1104 60-61 0.47059663812184027 1104 62-63 0.37256530394176934 1104 64-65 0.42774258095314366 1104 66-67 0.3710039587770524 1104 68-69 0.46184804596881435 1104 70-71 0.38857079155837937 1104 72-73 0.4817175727217631 1104 74-75 0.35324585027180433 1104 76-77 0.41108964017585237 1104 78-79 0.4734181889449971 1104 80-81 0.5761038297929417 1104 82-83 0.5344391332312064 1104 84-85 0.5615090418228057 1104 86-87 0.6707749854293503 1104 88-89 0.5929711539804536 1104 90-91 0.5744798914546045 1104 92-93 0.4175472612626372 1104 94-95 0.3617431644060609 1104 96-97 0.4412128190794071 1104 98-99 0.465092210366187 1104 100-101 0.7196934923731781 1105 1 0.25803259264322875 1105 2 0.29293871165615215 1105 3 0.3223037276891745 1105 4 0.2135322337324439 1105 5 0.21316048479403094 1105 6 0.29967689124201513 1105 7 0.3194240204559904 1105 8 0.3270173539962826 1105 9 0.32218157423159255 1105 10-11 0.46571165580972007 1105 12-13 0.411589603555889 1105 14-15 0.49623835890542267 1105 16-17 0.6141923420316644 1105 18-19 0.5483127103394949 1105 20-21 0.6059047322255751 1105 22-23 0.5224634672700788 1105 24-25 0.6461610002901992 1105 26-27 0.6785784760722464 1105 28-29 0.46917466070258484 1105 30-31 0.4522940650750158 1105 32-33 0.44183663305388876 1105 34-35 0.403925793945227 1105 36-37 0.5009712913482289 1105 38-39 0.6123748429614864 1105 40-41 0.5313305154141688 1105 42-43 0.6474159952102667 1105 44-45 0.4922112726394232 1105 46-47 0.3929963574347468 1105 48-49 0.5700624240130097 1105 50-51 0.6038690387214345 1105 52-53 0.5988573661734122 1105 54-55 0.5898028392880761 1105 56-57 0.6609766940041482 1105 58-59 0.5857534315882944 1105 60-61 0.5334795710188729 1105 62-63 0.5250184527961679 1105 64-65 0.6054052005774224 1105 66-67 0.6789492849350438 1105 68-69 0.7606046115799714 1105 70-71 0.6505928401528607 1105 72-73 0.7020715700150504 1105 74-75 0.7904857426026197 1105 76-77 0.5599385038958857 1105 78-79 0.6508374386881002 1105 80-81 0.6154574237503319 1105 82-83 0.5863319775051075 1105 84-85 0.6673540891642737 1105 86-87 0.7877098150802091 1105 88-89 0.82238848022185 1105 90-91 0.8345983119156557 1105 92-93 0.8539016390860716 1105 94-95 1.0978630991686025 1105 96-97 1.1143546884076976 1105 98-99 1.1543222297641051 1105 100-101 1.2678002571436444 1106 1 0.5251142444278578 1106 2 0.6122751396930006 1106 3 0.6113731706722589 1106 4 0.5383518629268096 1106 5 0.49640045985093906 1106 6 0.519696876630448 1106 7 0.5375667346762327 1106 8 0.5196106335081723 1106 9 0.5882386924955156 1106 10-11 0.5509418771410566 1106 12-13 0.6680353668853769 1106 14-15 0.847835109407292 1106 16-17 0.7726673475154584 1106 18-19 0.857762256150437 1106 20-21 0.9031823323620571 1106 22-23 0.8514296781724013 1106 24-25 0.830196437796026 1106 26-27 0.9610342237736518 1106 28-29 0.8022030414997303 1106 30-31 0.804630905065288 1106 32-33 0.8012096732285627 1106 34-35 0.8273861254458765 1106 36-37 0.8890544536287521 1106 38-39 0.9014973405524813 1106 40-41 0.8234322447268809 1106 42-43 0.8317574255387186 1106 44-45 0.9542506263778208 1106 46-47 0.8053534146487138 1106 48-49 0.9552968362125398 1106 50-51 1.0017045890537588 1106 52-53 1.0305716274303478 1106 54-55 1.0043012518109862 1106 56-57 1.041261720596836 1106 58-59 1.2067977424401448 1106 60-61 1.1233606157517855 1106 62-63 1.0900746966250452 1106 64-65 1.0890529381922178 1106 66-67 1.1250267809240029 1106 68-69 1.0940841794628184 1106 70-71 1.0211014034935246 1106 72-73 1.0500723475548455 1106 74-75 1.1673360406298272 1106 76-77 1.3162534883599548 1106 78-79 1.3155403418005847 1106 80-81 1.3263390873050582 1106 82-83 1.3273767460829866 1106 84-85 1.3585961852165944 1106 86-87 1.4146500235773907 1106 88-89 1.4244160579178704 1106 90-91 1.4623552938723314 1106 92-93 1.4574530877889522 1106 94-95 1.4703517734216298 1106 96-97 1.4679831183063676 1106 98-99 1.5996089594542937 1106 100-101 1.9587275922448733 1107 1 0.22103252002784401 1107 2 0.28289161297859877 1107 3 0.3699664410390895 1107 4 0.4169313093216829 1107 5 0.22930558722394778 1107 6 0.34743248473770194 1107 7 0.34859767424271837 1107 8 0.32194609803718066 1107 9 0.40499520093109 1107 10-11 0.503830089816681 1107 12-13 0.410468709069427 1107 14-15 0.41436999878039416 1107 16-17 0.40684206060482353 1107 18-19 0.4580201209930195 1107 20-21 0.5409654673972568 1107 22-23 0.6004431045646683 1107 24-25 0.62153805146378 1107 26-27 0.5829106593805022 1107 28-29 0.6031610258971867 1107 30-31 0.5510986975989383 1107 32-33 0.6036389131770292 1107 34-35 0.6899928432780982 1107 36-37 0.7118062669714149 1107 38-39 0.6608734776233476 1107 40-41 0.7069406957536373 1107 42-43 0.8103509909293365 1107 44-45 0.7772633952885002 1107 46-47 0.6950970530226002 1107 48-49 0.8371173296890007 1107 50-51 0.6297213829318764 1107 52-53 0.5220598398249976 1107 54-55 0.47995897925677156 1107 56-57 0.5236344002698416 1107 58-59 0.6018086091497992 1107 60-61 0.6374149872316934 1107 62-63 0.527605164855899 1107 64-65 0.696190476878968 1107 66-67 0.6474236525479071 1107 68-69 0.7969291922261093 1107 70-71 0.6324588360559957 1107 72-73 0.5022770884466894 1107 74-75 0.6715864905411095 1107 76-77 0.4176169899832871 1107 78-79 0.5552527919450725 1107 80-81 0.7222248400442126 1107 82-83 0.7238781853776324 1107 84-85 0.6949226051590749 1107 86-87 0.6106452160944578 1107 88-89 0.6985163407192481 1107 90-91 0.6503950293624321 1107 92-93 0.800965444368753 1107 94-95 0.9904695862557276 1107 96-97 0.8646362841223798 1107 98-99 1.0592581340198706 1107 100-101 1.5448752418184206 1108 1 0.13960243406872053 1108 2 0.21320399061270834 1108 3 0.10234908299683454 1108 4 0.05072200804921323 1108 5 0.12272573207852133 1108 6 0.13193467034227524 1108 7 0.12148259237244474 1108 8 -0.05130215521059256 1108 9 0.10590500378715717 1108 10-11 0.09932609286401117 1108 12-13 -0.1365813906879083 1108 14-15 0.2713680622361707 1108 16-17 0.19416615339911658 1108 18-19 0.2514591784527056 1108 20-21 0.2807330154978729 1108 22-23 0.3314542496829489 1108 24-25 0.4115598542346106 1108 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2316 80-81 -1.694671577421019 2316 82-83 -1.6638932685012016 2316 84-85 -1.4773941562533786 2316 86-87 -1.5707366300144834 2316 88-89 -1.8336689147732166 2316 90-91 -1.6556930428100287 2316 92-93 -2.0123945011784734 2316 94-95 -2.223871286735328 2316 96-97 -2.154037342401711 2316 98-99 -2.334951554223366 2316 100-101 -2.1421734619701134 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6043.0 3 1721.0 4 1759.0 5 1759.0 6 1919.0 7 2226.0 8 2707.0 9 3392.0 10 4160.0 11 5349.0 12 6693.0 13 8341.0 14 9654.0 15 11688.0 16 15099.0 17 19993.0 18 17362.0 19 11407.0 20 10901.0 21 11184.0 22 12126.0 23 13387.0 24 15178.0 25 17648.0 26 20312.0 27 23866.0 28 28926.0 29 34556.0 30 42756.0 31 54197.0 32 68885.0 33 89982.0 34 124220.0 35 177875.0 36 269756.0 37 410701.0 38 404997.0 39 80144.0 40 71.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03991277758431 19.694692157476425 14.78167212541455 22.483722939524714 2 14.840846908857147 20.722436068298343 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27.087373552072957 30.401508141505097 20.719115257613083 22-23 21.41854508786458 27.10469266685232 30.304248267801377 21.172513977481724 24-25 21.253291151733514 27.289712210500483 30.400874587373877 21.056122050392123 26-27 21.005742988763803 27.411972020683773 30.433310118454653 21.148974872097774 28-29 20.787731915461602 27.401310029230135 30.642169030262433 21.16878902504583 30-31 21.023462709869776 27.269321389025336 30.542195315097892 21.165020586007 32-33 20.861323806328862 27.48194709378206 30.363874108373757 21.292854991515316 34-35 21.27006638333382 27.4003203226635 30.17100607320213 21.15860722080055 36-37 21.315514186394342 27.171693228711103 30.164098289522528 21.348694295372024 38-39 20.94842379933708 27.581381203131244 30.224960232570897 21.24523476496078 40-41 21.0989064815155 27.44618737984154 30.32964745355914 21.12525868508382 42-43 21.028382645920402 27.435006691883167 29.951644562431657 21.584966099764774 44-45 21.129605836820836 27.481600159848497 29.826950779738908 21.561843223591755 46-47 21.390932875780607 27.712471396833465 29.397395612214268 21.499200115171664 48-49 21.274148735569646 27.933897370062237 29.18088164753638 21.611072246831732 50-51 21.25707763809106 28.058954693323567 29.053912457622307 21.630055210963068 52-53 21.364584254058762 28.22614232593056 28.820262725353796 21.58901069465688 54-55 21.334747329413258 27.99846912315429 28.88974209671361 21.777041450718844 56-57 21.382655985958003 28.356493016588434 28.573181162701232 21.68766983475233 58-59 21.491537726305303 27.96971796339247 28.853768491900407 21.684975818401817 60-61 21.505104870668365 28.010585630645537 29.10539079142208 21.37891870726402 62-63 21.253227759317888 28.088901993667907 29.23049532146328 21.427374925550925 64-65 21.243738860822194 28.086043484020184 29.020565257493597 21.649652397664028 66-67 21.252902982170554 28.31471400222516 28.527825893805037 21.90455712179925 68-69 21.174124679886358 28.129644984237355 28.937244747785467 21.758985588090816 70-71 21.36445791759806 28.07834644811499 28.868709623482587 21.688486010804365 72-73 21.346802649081845 28.034353658399265 28.814346402652802 21.80449728986609 74-75 21.170955795495747 28.494191271716478 28.573170292822187 21.76168263996559 76-77 21.20087872997466 28.25988057029301 28.648830237243384 21.890410462488948 78-79 21.255726074163146 28.163676638597014 28.58057034821143 22.00002693902841 80-81 21.228451573870334 28.427970895867542 28.421947730184705 21.92162980007742 82-83 21.384098924872326 28.32829915693259 28.570424511598357 21.717177406596722 84-85 21.210643624794372 28.232310034720037 28.4705036425793 22.086542697906292 86-87 21.104439830386852 28.384658601372365 28.608797959626507 21.90210360861428 88-89 21.288809639701718 28.127963321747263 28.465379338254042 22.117847700296974 90-91 21.197641481940085 28.037500593890837 28.637734427692507 22.127123496476575 92-93 21.28235845361807 28.084035659359408 28.60211872263329 22.031487164389237 94-95 21.15191010113035 27.827747722325835 28.712044275330474 22.30829790121334 96-97 21.07963257246067 27.91812863491463 28.8030629250405 22.1991758675842 98-99 20.93013966675529 28.033062266206134 28.615327688177643 22.42147037886093 100-101 20.86602558008012 28.027159109509757 28.735884545605135 22.370930764804996 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 541.0 1 415.5 2 331.5 3 402.0 4 415.5 5 428.0 6 648.5 7 1312.5 8 2191.0 9 2676.0 10 2514.5 11 2302.5 12 2428.0 13 2637.5 14 2813.5 15 2978.5 16 3137.0 17 3287.5 18 3486.5 19 3754.0 20 4178.0 21 4903.5 22 5893.0 23 7245.5 24 8862.0 25 11009.5 26 14133.0 27 17605.0 28 20294.5 29 23626.5 30 28445.5 31 33466.5 32 38905.0 33 44987.5 34 51791.5 35 57495.5 36 65056.5 37 71833.0 38 72504.0 39 71986.5 40 71103.0 41 70720.5 42 70033.5 43 71622.0 44 74664.5 45 75735.0 46 74637.5 47 74091.5 48 78097.0 49 76368.5 50 69223.5 51 64218.5 52 60127.5 53 57692.0 54 54765.0 55 49640.0 56 44435.5 57 38782.5 58 34165.0 59 30712.5 60 26311.5 61 21070.5 62 16253.5 63 12378.5 64 9230.0 65 6888.0 66 4999.5 67 3757.5 68 2775.0 69 1787.5 70 1206.5 71 850.5 72 572.0 73 391.5 74 282.5 75 221.0 76 160.5 77 88.0 78 47.0 79 36.0 80 29.0 81 23.5 82 15.5 83 9.0 84 10.5 85 12.5 86 11.0 87 9.0 88 7.0 89 5.0 90 3.0 91 2.5 92 2.0 93 1.0 94 0.0 95 1.0 96 2.0 97 1.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06182266733237394 2 0.09848551597207945 3 0.06940977219105798 4 0.13289670768598197 5 0.10847112494737976 6 0.10357621858693843 7 0.11395342007107404 8 0.09956239537137655 9 0.1208062889756919 10-11 0.11108989985021588 12-13 0.09295427178478076 14-15 0.09633175717348527 16-17 0.11082068000039161 18-19 0.09004180250031817 20-21 0.07278725757976251 22-23 0.07496549091015889 24-25 0.0632177156450997 26-27 0.056095626890657585 28-29 0.06767208043310131 30-31 0.049879095812897095 32-33 0.05017279019452358 34-35 0.04584079806553301 36-37 0.05159231303905156 38-39 0.05227759992951335 40-41 0.06681547182002408 42-43 0.06302191939068205 44-45 0.04926723251784193 46-47 0.037739728039002614 48-49 0.045693950874719766 50-51 0.028977845653812645 52-53 0.0413864332775314 54-55 0.04762743888709409 56-57 0.05215522727050231 58-59 0.05881229992070251 60-61 0.054602680450722973 62-63 0.06245900515923131 64-65 0.043050701440081454 66-67 0.06311981751789088 68-69 0.0532076321379972 70-71 0.02567378386051475 72-73 0.020607555777457976 74-75 0.030617639284560485 76-77 0.04415205537118075 78-79 0.06326666470870412 80-81 0.04035850294183872 82-83 0.06015839916982388 84-85 0.06478408568044093 86-87 0.06622808305677112 88-89 0.09004180250031817 90-91 0.06456381489422107 92-93 0.07677660626352217 94-95 0.08384974595435989 96-97 0.09224451036251677 98-99 0.12469773953224274 100-101 0.1197294095763948 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2042940.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.457996866423585 #Duplication Level Percentage of deduplicated Percentage of total 1 77.59217300084188 43.03106487139686 2 12.965468703250064 14.3807784543311 3 4.04469409360779 6.729318971068282 4 1.8007851674438609 3.994717526928148 5 0.9249523382256042 2.5648001937453366 6 0.5787467444466406 1.925768108398479 7 0.3704469752922783 1.4380973036454687 8 0.26385561805964053 1.170632323163184 9 0.19000424331496077 0.948352925733145 >10 1.0993421324136843 11.558676588112396 >50 0.10816210092719021 4.175298567001041 >100 0.058451864746164844 5.808127706107478 >500 0.002121467222071227 0.7320915770913089 >1k 6.18761273104105E-4 0.693217288847473 >5k 1.7678893517260146E-4 0.8490575944302546 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9845 0.48190353118544843 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7475 0.365894250442989 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4630 0.22663416448843332 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2537 0.12418377436439641 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.894906360441325E-5 2 0.0 0.0 0.0 0.0 4.894906360441325E-5 3 0.0 0.0 0.0 0.0 4.894906360441325E-5 4 0.0 0.0 0.0 0.0 4.894906360441325E-5 5 0.0 0.0 0.0 0.0 4.894906360441325E-5 6 0.0 0.0 0.0 4.894906360441325E-5 4.894906360441325E-5 7 0.0 0.0 0.0 4.894906360441325E-5 4.894906360441325E-5 8 0.0 0.0 0.0 4.894906360441325E-5 4.894906360441325E-5 9 0.0 0.0 0.0 4.894906360441325E-5 4.894906360441325E-5 10-11 0.0 0.0 0.0 9.78981272088265E-5 4.894906360441325E-5 12-13 0.0 0.0 0.0 1.4684719081323975E-4 4.894906360441325E-5 14-15 0.0 0.0 0.0 1.95796254417653E-4 4.894906360441325E-5 16-17 0.0 0.0 0.0 2.2027078621985962E-4 4.894906360441325E-5 18-19 0.0 0.0 0.0 2.4474531802206624E-4 4.894906360441325E-5 20-21 0.0 0.0 0.0 3.6711797703309937E-4 4.894906360441325E-5 22-23 0.0 0.0 0.0 6.608123586595788E-4 4.894906360441325E-5 24-25 0.0 0.0 0.0 0.0010768793992970915 4.894906360441325E-5 26-27 0.0 0.0 0.0 0.0016153190989456373 9.78981272088265E-5 28-29 0.0 0.0 0.0 0.0026187749028361088 9.78981272088265E-5 30-31 0.0 0.0 0.0 0.005457820591892077 9.78981272088265E-5 32-33 0.0 0.0 0.0 0.009642965530069408 9.78981272088265E-5 34-35 0.0 0.0 0.0 0.013387568895807023 9.78981272088265E-5 36-37 0.0 0.0 0.0 0.017230070388753464 9.78981272088265E-5 38-39 0.0 0.0 0.0 0.021757858772161687 9.78981272088265E-5 40-41 0.0 0.0 0.0 0.028610727676779545 9.78981272088265E-5 42-43 0.0 0.0 0.0 0.03800894788882689 9.78981272088265E-5 44-45 0.0 0.0 0.0 0.04701557559203892 9.78981272088265E-5 46-47 0.0 0.0 0.0 0.05665854112210833 9.78981272088265E-5 48-49 0.0 0.0 0.0 0.06590991414334244 1.2237265901103312E-4 50-51 0.0 0.0 0.0 0.07878351787130312 1.4684719081323975E-4 52-53 0.0 0.0 0.0 0.1071739747618628 1.4684719081323975E-4 54-55 0.0 0.0 0.0 0.1441060432513926 1.4684719081323975E-4 56-57 0.0 0.0 0.0 0.18096468814551575 1.4684719081323975E-4 58-59 0.0 0.0 0.0 0.23921407383476753 1.4684719081323975E-4 60-61 0.0 0.0 0.0 0.3089420149392542 1.4684719081323975E-4 62-63 0.0 0.0 0.0 0.3550030837910071 1.4684719081323975E-4 64-65 0.0 0.0 0.0 0.39301203167983395 1.4684719081323975E-4 66-67 0.0 0.0 0.0 0.497493807943454 1.4684719081323975E-4 68-69 0.0 0.0 0.0 0.6059159838272294 1.4684719081323975E-4 70-71 0.0 0.0 0.0 0.656676162785006 1.4684719081323975E-4 72-73 0.0 0.0 0.0 0.7489940967429294 1.4684719081323975E-4 74-75 0.0 0.0 0.0 0.8889394695879468 1.4684719081323975E-4 76-77 0.0 0.0 0.0 1.0281995555425025 1.4684719081323975E-4 78-79 0.0 0.0 0.0 1.1596767403839565 1.4684719081323975E-4 80-81 0.0 0.0 0.0 1.3173416742537716 1.4684719081323975E-4 82-83 0.0 0.0 0.0 1.4608113796783067 1.4684719081323975E-4 84-85 0.0 0.0 0.0 1.6206055978149139 1.7132172261544637E-4 86-87 0.0 0.0 0.0 1.893912694450155 1.95796254417653E-4 88-89 0.0 0.0 0.0 2.263796293576904 1.95796254417653E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11505 0.0 35.894424 1 ATCAACG 13795 0.0 29.726994 3 TCAACGC 13890 0.0 29.523676 4 CAACGCA 13975 0.0 29.276098 5 TATCAAC 14110 0.0 28.998125 2 GGTATCA 7175 0.0 28.678724 1 AACGCAG 14295 0.0 28.586798 6 GTACATG 9635 0.0 26.436678 1 ACGCAGA 15940 0.0 25.398174 7 CGCAGAG 16095 0.0 25.035492 8 TACATGG 9960 0.0 24.81063 2 ACATGGG 9765 0.0 24.136335 3 GCAGAGT 16685 0.0 23.324318 9 GAGTACT 7535 0.0 22.981888 12-13 AGAGTAC 12750 0.0 22.135546 10-11 CATGGGA 6195 0.0 21.400541 4 GGACCGT 245 7.496055E-9 21.334272 6 GTACTTT 8185 0.0 21.069492 14-15 AGTACTT 8310 0.0 20.752813 12-13 CATGGGG 3825 0.0 19.007364 4 >>END_MODULE