FastQCFastQC Report
Thu 26 May 2016
SRR937051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042940
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123010.6021224313978873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98890.48405728998404257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64110.3138124467678933No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33220.1626087892938608No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25730.1259459406541553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96650.034.1114121
GTATCAA144900.031.5718461
ATCAACG177300.025.611683
TCAACGC178100.025.5220574
CAACGCA178850.025.3619175
TATCAAC180100.025.240492
AACGCAG182600.024.8156626
GTGTAGC10350.023.8673971
GAGTACT112650.023.46426412-13
GTACATG94250.022.731981
CGCAGAG203400.022.0678068
ACGCAGA204450.022.0241687
GTACTTT119750.021.87501114-15
AGTACTT119700.021.54659512-13
ACATGGG93500.021.0826593
AGAGTAC164550.021.07187810-11
TACATGG98750.020.8762342
GGACCGA2056.836908E-720.8529226
GCAGAGT214800.020.5428039
ACTTTTT138200.019.12696516-17
Produced by FastQC (version 0.11.5)