FastQCFastQC Report
Thu 26 May 2016
SRR937050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953236
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96470.4938983307700657No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70680.36186103471367514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45650.23371471752517362No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23990.12282181979033767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA112300.033.302931
GGTATCA72650.031.5281771
ATCAACG135400.027.3397853
TCAACGC135900.027.2391994
CAACGCA136300.027.1285655
TATCAAC137200.026.9824832
GTACATG85500.026.511831
AACGCAG140150.026.2802476
TACATGG87400.025.3924142
ACATGGG85000.024.5426753
ACGCAGA156650.023.3616437
CGCAGAG157000.023.1587948
GAGTACT77700.023.11842312-13
TAGACCG1553.890945E-521.463945
GCAGAGT163850.021.3493969
AGAGTAC123850.021.27723710-11
GTACTTT84300.021.08322314-15
CATGGGG32600.020.8446564
AGTACTT84300.020.7729112-13
CATGGGA56300.020.2571874
Produced by FastQC (version 0.11.5)