##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937050_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1953236 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.820920257459928 33.0 31.0 34.0 30.0 34.0 2 31.942295247476494 33.0 31.0 34.0 30.0 34.0 3 31.953943609476788 33.0 31.0 34.0 30.0 34.0 4 35.52916135070212 37.0 35.0 37.0 33.0 37.0 5 35.53319260959761 37.0 35.0 37.0 33.0 37.0 6 35.60983823767328 37.0 35.0 37.0 33.0 37.0 7 35.54933454021941 37.0 35.0 37.0 33.0 37.0 8 35.50168131244765 37.0 35.0 37.0 33.0 37.0 9 37.1493383288041 39.0 37.0 39.0 34.0 39.0 10-11 36.95939737952813 39.0 37.0 39.0 33.0 39.0 12-13 36.904123464855246 39.0 37.0 39.0 33.0 39.0 14-15 38.08373181735335 40.0 38.0 41.0 33.0 41.0 16-17 38.022457091718564 40.0 38.0 41.0 33.0 41.0 18-19 38.0099125246514 40.0 38.0 41.0 33.0 41.0 20-21 37.94234618858141 40.0 38.0 41.0 33.0 41.0 22-23 37.837225506799996 40.0 38.0 41.0 33.0 41.0 24-25 37.68573920406955 40.0 37.0 41.0 32.0 41.0 26-27 37.78592039057236 40.0 38.0 41.0 33.0 41.0 28-29 37.74488438673053 40.0 38.0 41.0 33.0 41.0 30-31 37.597128048018774 40.0 37.0 41.0 32.0 41.0 32-33 37.394882390044 40.0 37.0 41.0 32.0 41.0 34-35 37.234545646301825 40.0 36.5 41.0 31.0 41.0 36-37 37.05865087475348 40.0 36.0 41.0 31.0 41.0 38-39 36.90117425646466 39.5 36.0 41.0 31.0 41.0 40-41 36.74447506599305 39.0 36.0 41.0 30.0 41.0 42-43 36.530904355643656 39.0 36.0 41.0 30.0 41.0 44-45 36.26283536654045 39.0 35.0 41.0 30.0 41.0 46-47 35.89722977663733 39.0 35.0 41.0 28.5 41.0 48-49 35.56970125473829 39.0 35.0 40.0 27.0 41.0 50-51 34.92072514534854 38.5 34.0 40.0 26.5 40.5 52-53 35.15246544708371 38.5 35.0 40.0 27.0 40.5 54-55 35.543771720365584 39.0 35.0 40.5 27.0 41.0 56-57 35.44376741981 39.0 35.0 41.0 27.0 41.0 58-59 35.22007069294238 39.0 35.0 41.0 26.0 41.0 60-61 34.95376800345683 38.5 35.0 40.0 26.0 41.0 62-63 34.64923772652153 38.0 34.0 40.0 26.0 41.0 64-65 34.35181104587464 38.0 34.0 40.0 25.0 41.0 66-67 33.982546911893905 37.0 34.0 40.0 23.5 41.0 68-69 33.605268129401665 36.5 33.5 39.0 22.5 41.0 70-71 33.202590214392934 36.0 33.0 39.0 22.0 41.0 72-73 32.80750482788562 36.0 33.0 39.0 21.5 40.0 74-75 32.380368782881334 35.0 32.0 38.0 20.0 40.0 76-77 31.947120829229036 35.0 32.0 37.0 19.0 39.0 78-79 31.501910931397948 35.0 31.5 37.0 17.5 39.0 80-81 31.051386519601316 35.0 31.0 36.0 14.0 38.5 82-83 30.637180555754654 35.0 31.0 36.0 9.5 37.0 84-85 30.26105370779568 34.5 31.0 35.0 7.0 37.0 86-87 29.93912896342275 34.0 30.5 35.0 4.0 36.5 88-89 29.58361636791458 34.0 30.0 35.0 2.0 36.0 90-91 29.22851027730392 34.0 30.0 35.0 2.0 36.0 92-93 28.8689938133436 34.0 29.0 35.0 2.0 35.5 94-95 28.460849840981837 34.0 29.0 35.0 2.0 35.0 96-97 28.017466911320497 34.0 29.0 35.0 2.0 35.0 98-99 27.43877135174654 34.0 28.0 35.0 2.0 35.0 100-101 25.19908244574644 31.5 22.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3915513150748282 1101 2 0.3746882285692159 1101 3 0.33758277457341634 1101 4 0.32729624878498953 1101 5 0.34740680661395373 1101 6 0.2648031662940653 1101 7 0.18021555600820705 1101 8 0.28594743673778567 1101 9 0.32468900445324067 1101 10-11 0.37914711800883083 1101 12-13 0.3782240524301912 1101 14-15 0.5806073629531596 1101 16-17 0.623890527203983 1101 18-19 0.6471183087100769 1101 20-21 0.5988412363435529 1101 22-23 0.6020191375033974 1101 24-25 0.576173759124984 1101 26-27 0.6659335916966 1101 28-29 0.6900225161959881 1101 30-31 0.6199065128660948 1101 32-33 0.749123741369452 1101 34-35 0.6699038344002091 1101 36-37 0.7154091772851032 1101 38-39 0.6859646538528139 1101 40-41 0.8218285583346301 1101 42-43 0.7470104739290164 1101 44-45 0.9161810002950617 1101 46-47 0.7919605526128422 1101 48-49 0.9121899080355576 1101 50-51 0.9485289351287634 1101 52-53 1.032047226217145 1101 54-55 1.0613701722579876 1101 56-57 1.0526930044722107 1101 58-59 1.0665779827034427 1101 60-61 1.1565539914340093 1101 62-63 1.203728225321747 1101 64-65 1.2645879204953516 1101 66-67 1.111857159488551 1101 68-69 1.2958724796314556 1101 70-71 1.1929803258785014 1101 72-73 1.0928234229685216 1101 74-75 0.8598986626703038 1101 76-77 0.9698846194367157 1101 78-79 1.0892729362130673 1101 80-81 0.8547591460319239 1101 82-83 1.1377681960053927 1101 84-85 1.1813994663947796 1101 86-87 1.0834658995940494 1101 88-89 1.1552130852658102 1101 90-91 1.333228515751852 1101 92-93 1.454243436501958 1101 94-95 1.3830952068877878 1101 96-97 1.2806553953582096 1101 98-99 1.3981270350159818 1101 100-101 1.624636918549946 1102 1 0.3430593843938041 1102 2 0.32243582329144616 1102 3 0.28684808488989333 1102 4 0.2646468363689749 1102 5 0.2511956957824424 1102 6 0.22494150646367217 1102 7 0.28347264182650633 1102 8 0.28071275963636566 1102 9 0.439025676236632 1102 10-11 0.5338665581893451 1102 12-13 0.47286266311490266 1102 14-15 0.4324861847654802 1102 16-17 0.5690522386209835 1102 18-19 0.3846051499323835 1102 20-21 0.5075630943839329 1102 22-23 0.516074756668452 1102 24-25 0.5774676182222649 1102 26-27 0.5825462601918616 1102 28-29 0.5362926056127648 1102 30-31 0.5951821656672962 1102 32-33 0.6851380052747729 1102 34-35 0.5451570491262956 1102 36-37 0.3472150438980819 1102 38-39 0.5841054915689128 1102 40-41 0.4320521946448892 1102 42-43 0.4948101795079225 1102 44-45 0.39894771980653587 1102 46-47 0.5087619001555623 1102 48-49 0.6870856863222201 1102 50-51 0.679813588324194 1102 52-53 0.6184917454551737 1102 54-55 0.5629258962738604 1102 56-57 0.7274748623448346 1102 58-59 0.6899656392457132 1102 60-61 0.5247337078335192 1102 62-63 0.5987683622149689 1102 64-65 0.7614091058128807 1102 66-67 0.46889070965337964 1102 68-69 0.31963849035140157 1102 70-71 0.46513084761617307 1102 72-73 0.7011130859360506 1102 74-75 0.5834586939577804 1102 76-77 0.677344283326935 1102 78-79 0.6949887737194622 1102 80-81 0.7032976829016633 1102 82-83 0.6425475584285394 1102 84-85 0.68990679966101 1102 86-87 0.5856418161999066 1102 88-89 0.6124743189824784 1102 90-91 0.6818378269239176 1102 92-93 0.8861821491016713 1102 94-95 0.8982812751498628 1102 96-97 0.798656708090455 1102 98-99 0.7304545868751973 1102 100-101 0.7591327329052824 1103 1 0.3351447686830227 1103 2 0.3967610818501548 1103 3 0.4131466395421093 1103 4 0.2638840651358194 1103 5 0.2994784235076793 1103 6 0.2402441722454327 1103 7 0.32616939532464784 1103 8 0.3750140980579104 1103 9 0.333055781503667 1103 10-11 0.4666493596568131 1103 12-13 0.43485142203311256 1103 14-15 0.5148504990548801 1103 16-17 0.5007847893648574 1103 18-19 0.42095719074870885 1103 20-21 0.559661406608555 1103 22-23 0.5833616646565716 1103 24-25 0.638498534653877 1103 26-27 0.6368631766603485 1103 28-29 0.5867351742391307 1103 30-31 0.4898909299318035 1103 32-33 0.711792366206133 1103 34-35 0.7537888080629358 1103 36-37 0.5886240736346409 1103 38-39 0.5826773118959565 1103 40-41 0.770688631604493 1103 42-43 0.7271155000101004 1103 44-45 0.903787053914165 1103 46-47 0.8876132035495559 1103 48-49 1.1002357702040086 1103 50-51 0.9182694720326907 1103 52-53 0.9309427760028868 1103 54-55 1.0081417536195048 1103 56-57 1.1347272958385588 1103 58-59 1.0539256202570897 1103 60-61 1.1215324883213214 1103 62-63 1.1152727959667885 1103 64-65 1.0268702021755445 1103 66-67 1.1129985899328574 1103 68-69 1.160430159322516 1103 70-71 0.9505777025098894 1103 72-73 1.0175934382395724 1103 74-75 1.0038038306574464 1103 76-77 1.0255153607009682 1103 78-79 0.9083365789662174 1103 80-81 0.9278491227187828 1103 82-83 0.9756533278365715 1103 84-85 0.714483127482108 1103 86-87 0.7327950202999709 1103 88-89 0.6955276622710151 1103 90-91 0.9029084028507661 1103 92-93 0.8883103038866196 1103 94-95 0.7945155818806846 1103 96-97 0.9149816255913308 1103 98-99 1.0939986155977977 1103 100-101 1.3668228956912607 1104 1 0.3983019736574889 1104 2 0.27255478279234424 1104 3 0.4416192420574241 1104 4 0.302580818251883 1104 5 0.35068108579336865 1104 6 0.22046378273169154 1104 7 0.23540595401148323 1104 8 0.13749917886727303 1104 9 0.22959967785800472 1104 10-11 0.4820209235624304 1104 12-13 0.2997635918802004 1104 14-15 0.48327704515108394 1104 16-17 0.5098128304224616 1104 18-19 0.5224252391079531 1104 20-21 0.49955029081691293 1104 22-23 0.3673839720644523 1104 24-25 0.5180160655804755 1104 26-27 0.4020278611223915 1104 28-29 0.45622162644389874 1104 30-31 0.5334849220029838 1104 32-33 0.4926073401589832 1104 34-35 0.4300046928469996 1104 36-37 0.5591673896032106 1104 38-39 0.6090722269677329 1104 40-41 0.5732053527993415 1104 42-43 0.5629250679503457 1104 44-45 0.6081631158094964 1104 46-47 0.6410882633433275 1104 48-49 0.6712579044322524 1104 50-51 0.7502733007065601 1104 52-53 0.7056716562796126 1104 54-55 0.6402546386516335 1104 56-57 0.5360661615588143 1104 58-59 0.4661078707497168 1104 60-61 0.527076108524362 1104 62-63 0.5797859505974756 1104 64-65 0.6738234109131653 1104 66-67 0.46945444551041504 1104 68-69 0.5349662017468404 1104 70-71 0.5080529776080667 1104 72-73 0.46215583205003696 1104 74-75 0.4753405892029292 1104 76-77 0.546136139447615 1104 78-79 0.5363928483026577 1104 80-81 0.6052395866061531 1104 82-83 0.5804103849062798 1104 84-85 0.4764685616715134 1104 86-87 0.5523888906773671 1104 88-89 0.5523834691710512 1104 90-91 0.7227592343469915 1104 92-93 0.6804839890086178 1104 94-95 0.7798870521756953 1104 96-97 0.7903564190629275 1104 98-99 0.8050137758357536 1104 100-101 0.9692130085388513 1105 1 0.2899402902073547 1105 2 0.3534307940121941 1105 3 0.356027111320774 1105 4 0.3086476939852716 1105 5 0.2738313330379185 1105 6 0.3544262866849408 1105 7 0.3867979422832519 1105 8 0.309715276216302 1105 9 0.3785050332960509 1105 10-11 0.4942801446164964 1105 12-13 0.458229463856874 1105 14-15 0.4819434320112066 1105 16-17 0.27181919109506225 1105 18-19 0.3593960574534094 1105 20-21 0.48442285568719967 1105 22-23 0.48282308606295743 1105 24-25 0.5013696947402622 1105 26-27 0.3649971897935913 1105 28-29 0.32010308502415796 1105 30-31 0.1314026293233752 1105 32-33 0.24975245630332665 1105 34-35 0.19121109253487134 1105 36-37 0.14316639648703955 1105 38-39 0.14424450011014756 1105 40-41 0.25950700001255456 1105 42-43 0.36145933489169835 1105 44-45 0.2081727218438587 1105 46-47 0.1859335175189969 1105 48-49 0.20019926033139512 1105 50-51 0.21299213355860758 1105 52-53 0.08230608608018741 1105 54-55 0.20210360814582629 1105 56-57 0.4126728077523154 1105 58-59 0.3403047323843964 1105 60-61 0.305567754230168 1105 62-63 0.353129205240144 1105 64-65 0.3583455772069115 1105 66-67 0.45538943778125685 1105 68-69 0.5332356377100638 1105 70-71 0.5244590498040651 1105 72-73 0.5372689615778086 1105 74-75 0.6165730877472413 1105 76-77 0.44922647459477005 1105 78-79 0.3155600199018984 1105 80-81 0.44264090307621373 1105 82-83 0.45757565894427543 1105 84-85 0.35716368387504716 1105 86-87 0.2994348134330238 1105 88-89 0.46196290220812486 1105 90-91 0.279199710924523 1105 92-93 0.13593548740219674 1105 94-95 0.39113778740679095 1105 96-97 0.4794791368908413 1105 98-99 0.4565411352994744 1105 100-101 0.4307275402520645 1106 1 0.4334272802023946 1106 2 0.3731210944004921 1106 3 0.3516226299808771 1106 4 0.2992190035681901 1106 5 0.27791688215741317 1106 6 0.313844899254498 1106 7 0.3593595052701417 1106 8 0.39709586056719814 1106 9 0.40783242509093753 1106 10-11 0.5992363923857695 1106 12-13 0.45421218402704966 1106 14-15 0.6747155450158715 1106 16-17 0.6355814072770443 1106 18-19 0.4778958030262501 1106 20-21 0.6119673410856166 1106 22-23 0.5003198719311115 1106 24-25 0.6887035343043522 1106 26-27 0.530669645718838 1106 28-29 0.6611858667564618 1106 30-31 0.674746099819238 1106 32-33 0.7504216322228459 1106 34-35 0.8047783391540051 1106 36-37 0.683113170395238 1106 38-39 0.7424109478246024 1106 40-41 0.8210467171689544 1106 42-43 0.7627323603778677 1106 44-45 0.6988020346578523 1106 46-47 0.6248217239051002 1106 48-49 0.6798514188899816 1106 50-51 0.7912665528333349 1106 52-53 0.7346342057386224 1106 54-55 0.7030888075691522 1106 56-57 0.7884168831243485 1106 58-59 0.8301226843673035 1106 60-61 0.8803563333084199 1106 62-63 0.8396895657293655 1106 64-65 0.8242015218194751 1106 66-67 0.9701711933525559 1106 68-69 1.0125965824064664 1106 70-71 0.8945073447657279 1106 72-73 1.0934908603811593 1106 74-75 0.9182492613545108 1106 76-77 1.0671501231793705 1106 78-79 1.1361490110597465 1106 80-81 1.1158193877443665 1106 82-83 1.1417537120957668 1106 84-85 1.1811964243891246 1106 86-87 1.068862901574665 1106 88-89 1.1007978277501103 1106 90-91 0.9939533374952738 1106 92-93 1.0210901164877875 1106 94-95 1.0597206709441735 1106 96-97 1.2073236991820302 1106 98-99 1.2104531024781977 1106 100-101 1.343315131119514 1107 1 0.2209750904251706 1107 2 0.18791486009445535 1107 3 0.18200897351312406 1107 4 0.2114027353812986 1107 5 0.32161382119160464 1107 6 0.2632939813448374 1107 7 0.2909584813506001 1107 8 0.4195797080893868 1107 9 0.3753711349221973 1107 10-11 0.5123785696885079 1107 12-13 0.4051654482456897 1107 14-15 0.49843024021192406 1107 16-17 0.631487306790504 1107 18-19 0.4989109664012332 1107 20-21 0.6016564325560196 1107 22-23 0.5045871595375431 1107 24-25 0.5320473580679916 1107 26-27 0.3846393777736239 1107 28-29 0.6132528353113429 1107 30-31 0.5692894864198621 1107 32-33 0.6301736555181279 1107 34-35 0.6170463697080848 1107 36-37 0.5663395137697123 1107 38-39 0.3307530522668358 1107 40-41 0.48378685294335355 1107 42-43 0.3720569987377047 1107 44-45 0.5516673850785097 1107 46-47 0.4637392362783004 1107 48-49 0.6703990953678414 1107 50-51 0.5302586826799356 1107 52-53 0.6846252595302573 1107 54-55 0.7886796391936173 1107 56-57 0.8334267096109329 1107 58-59 0.9809803191762612 1107 60-61 0.9710190734441966 1107 62-63 0.811445901850071 1107 64-65 0.9267406673077758 1107 66-67 0.7818416745581658 1107 68-69 0.698525505408476 1107 70-71 0.5846881370591461 1107 72-73 0.7807791050633668 1107 74-75 0.8485789156866446 1107 76-77 0.8660886618314727 1107 78-79 0.8303138242417596 1107 80-81 0.7190423175756173 1107 82-83 0.6877427808689767 1107 84-85 0.7362209843306928 1107 86-87 0.7996775874121553 1107 88-89 0.7388921455273234 1107 90-91 0.8981862386735564 1107 92-93 0.8398528433207808 1107 94-95 0.7781894099518993 1107 96-97 0.7033100966425287 1107 98-99 0.7619714300141283 1107 100-101 0.6686213191347505 1108 1 0.16646374613850057 1108 2 0.21724374646071354 1108 3 0.1705265562784497 1108 4 0.09863042980634873 1108 5 0.03831054172081849 1108 6 0.007679601701767069 1108 7 0.06518532690053291 1108 8 0.10711832186125747 1108 9 0.2010804981654033 1108 10-11 0.07645859500406971 1108 12-13 0.17350542021774373 1108 14-15 0.3240177974105336 1108 16-17 0.30883565198632823 1108 18-19 0.23658344124891073 1108 20-21 0.2686550311586444 1108 22-23 0.18778093914144023 1108 24-25 0.24803584551317925 1108 26-27 0.2148241713563337 1108 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2316 98-99 -3.853310437539477 2316 100-101 -4.261168485741688 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4397.0 3 1483.0 4 1544.0 5 1539.0 6 1707.0 7 2017.0 8 2474.0 9 3023.0 10 3864.0 11 4950.0 12 6431.0 13 7930.0 14 8938.0 15 11210.0 16 14496.0 17 19614.0 18 17020.0 19 10787.0 20 10047.0 21 10315.0 22 11282.0 23 12537.0 24 14095.0 25 16265.0 26 18658.0 27 22296.0 28 26804.0 29 32097.0 30 39429.0 31 49321.0 32 63059.0 33 83874.0 34 114256.0 35 166721.0 36 256895.0 37 401012.0 38 401459.0 39 79330.0 40 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.03633760759277 19.64320561774445 14.868174053785499 22.452282720877285 2 14.763438932568443 20.731221348631138 41.31533792608907 23.19000179271135 3 18.44076341006764 26.024626263371147 30.483860395183726 25.050749931377492 4 12.096404080470249 17.32314923137758 38.984973805577354 31.59547288257482 5 12.852636632045753 38.07125661469858 34.81540834764842 14.260698405607247 6 29.75308313079688 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21.06958823922479 28-29 20.713286827873986 27.393441204287594 30.782471507597204 21.11080046024121 30-31 20.9383442261333 27.180292395385088 30.71441884471462 21.166944533766994 32-33 20.76722211270887 27.405770870187425 30.61282535066318 21.214181666440528 34-35 21.23133553625827 27.3531951585489 30.368285458928902 21.047183846263934 36-37 21.2464128 27.090022400000002 30.363852800000004 21.299712 38-39 20.82617258552128 27.50464461911563 30.446326328366187 21.2228564669969 40-41 20.97721747643205 27.403823507124148 30.54148942488669 21.07746959155711 42-43 20.89942620101243 27.36783602466409 30.16328208720086 21.569455687122623 44-45 21.01493906669104 27.42139697860077 29.99184415736015 21.571819797348045 46-47 21.24834820059462 27.661758584621026 29.589032819929418 21.500860394854936 48-49 21.23046078538536 27.876411227719156 29.325301124630386 21.567826862265097 50-51 21.192551595817804 27.948975200573244 29.238854645422034 21.619618558186914 52-53 21.32468424150248 28.176100274715075 28.94917323075529 21.55004225302715 54-55 21.276583216636418 27.95331952597584 29.049622004387977 21.720475252999773 56-57 21.324727006715403 28.271572979534753 28.77150909525262 21.632190918497226 58-59 21.44472929790364 27.94960295455674 29.002186834854797 21.60348091268482 60-61 21.4510426350863 27.984964281078124 29.290053622549788 21.27393946128579 62-63 21.221455309716276 28.028784522903983 29.39130519382598 21.358454973553766 64-65 21.179807512851294 28.06570461494506 29.160935139465245 21.593552732738406 66-67 21.166835349116344 28.253290459912932 28.708080192310792 21.87179399865993 68-69 21.09739182136182 28.11332296640855 29.075485568713226 21.7137996435164 70-71 21.28162442225229 27.97376848715872 29.085276740258557 21.659330350330443 72-73 21.208264395944614 28.003993119830977 28.965045607491373 21.822696876733037 74-75 21.063925446513384 28.448915509769552 28.77369674010281 21.71346230361425 76-77 21.135086928233605 28.164932045189005 28.86000968387103 21.839971342706356 78-79 21.17437731533291 28.125971524318132 28.78930976254217 21.910341397806782 80-81 21.175534585896465 28.38417000429425 28.579498358478745 21.86079705133054 82-83 21.28262197760665 28.2733109601241 28.73440631188759 21.709660750381666 84-85 21.125086573186827 28.21606107042225 28.59589492671524 22.062957429675688 86-87 21.052829682372558 28.323350717560253 28.738155032344494 21.885664567722692 88-89 21.226573305538015 28.12084100802572 28.575510523865926 22.07707516257034 90-91 21.074581605330792 28.022419184783914 28.851271531649907 22.051727678235387 92-93 21.224414752630384 28.005656006344314 28.78581512121312 21.98411411981218 94-95 20.971592558851228 27.799046786527605 28.921174766215866 22.3081858884053 96-97 20.977119152546408 27.852820342491835 28.995287798699554 22.1747727062622 98-99 20.822236599525343 28.023703084443774 28.79843823556682 22.355622080464062 100-101 20.758753662755712 27.984311788726078 28.922545924853665 22.334388623664548 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 390.0 1 310.0 2 277.5 3 356.0 4 346.5 5 350.5 6 577.5 7 1237.0 8 2163.5 9 2637.0 10 2426.0 11 2211.0 12 2406.5 13 2632.5 14 2761.5 15 2862.5 16 3021.0 17 3241.0 18 3406.5 19 3674.0 20 4080.0 21 4729.0 22 5659.0 23 7007.0 24 8525.5 25 10632.0 26 13840.0 27 17188.0 28 19670.0 29 22886.0 30 27718.0 31 32478.5 32 37560.5 33 43183.5 34 49677.5 35 55390.5 36 62594.5 37 69388.5 38 70345.0 39 69325.5 40 68074.5 41 67806.0 42 67282.5 43 68687.0 44 71554.5 45 72634.5 46 71485.0 47 70936.0 48 74783.5 49 73181.5 50 66217.5 51 61012.0 52 56953.5 53 54404.5 54 51783.0 55 47100.0 56 41629.0 57 36405.0 58 32285.0 59 28679.5 60 24452.5 61 19768.5 62 15137.5 63 11366.5 64 8420.0 65 6263.5 66 4573.0 67 3506.0 68 2599.0 69 1653.5 70 1091.0 71 774.5 72 531.5 73 367.5 74 277.0 75 207.5 76 139.0 77 65.5 78 34.5 79 24.5 80 18.0 81 14.5 82 17.0 83 18.0 84 9.0 85 7.5 86 8.0 87 6.0 88 4.5 89 1.5 90 1.0 91 2.0 92 2.5 93 3.5 94 2.0 95 0.5 96 0.5 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04356872390228318 2 0.045360622065126796 3 0.026827275352287176 4 0.04669174641466776 5 0.06824572145915803 6 0.0677849476458554 7 0.06839931273025891 8 0.07065198470640517 9 0.06993522544126772 10-11 0.07664204427933952 12-13 0.07761478899631177 14-15 0.0718807148752122 16-17 0.05936302628049043 18-19 0.05193944817728119 20-21 0.05813429611168338 22-23 0.03220296984081801 24-25 0.017842185992885652 26-27 0.013797615853895792 28-29 0.017765390357335212 30-31 0.01351603185687751 32-33 0.007730760645411 34-35 0.011596140968116501 36-37 0.0056828770307325895 38-39 0.008677906817199765 40-41 0.008191534458713642 42-43 0.00578527121146651 44-45 0.0017918981628436091 46-47 0.0032254166931184967 48-49 8.447519910548443E-4 50-51 0.007500373738759679 52-53 0.0062204464795856725 54-55 0.008754702452750205 56-57 0.024907384463526167 58-59 0.009471461717887649 60-61 0.021477179408939832 62-63 0.015947893649308122 64-65 0.011391352606648659 66-67 0.018200565625454374 68-69 0.028235195337378588 70-71 0.020171653604582347 72-73 0.01930130306834402 74-75 0.019608485610545782 76-77 0.025828932090131457 78-79 0.03906337994999068 80-81 0.0320749771149006 82-83 0.030871845491277042 84-85 0.020914011414903268 86-87 0.026008121906415816 88-89 0.019915668152747545 90-91 0.018174967080270894 92-93 0.03181899166306581 94-95 0.024216223743572206 96-97 0.03847461341077064 98-99 0.055548843048151886 100-101 0.06781054619103886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1953236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.057139876091824 #Duplication Level Percentage of deduplicated Percentage of total 1 78.24145270731141 43.8599205852238 2 12.617590928282763 14.146121191321082 3 3.977368164780165 6.68879650655389 4 1.6720118337014966 3.7491280494514236 5 0.9069215850154266 2.5419715073928337 6 0.5327142471469932 1.791746223978357 7 0.3547071068442598 1.3918706132388765 8 0.2627783400746531 1.1784481732777645 9 0.18342947501094845 0.9254278564278144 >10 1.0825721113744236 11.435424186564875 >50 0.10679343018238668 4.153312644365538 >100 0.058637462034355665 5.8131324974874365 >500 0.0021982605386773736 0.7529756379221718 >1k 6.411593237808997E-4 0.7134912693192286 >5k 1.831883782231142E-4 0.8582330574748781 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9647 0.4938983307700657 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7068 0.36186103471367514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4565 0.23371471752517362 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2399 0.12282181979033767 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.67956355504404E-5 2.5598545183480132E-5 12-13 0.0 0.0 0.0 1.2799272591740066E-4 5.1197090366960264E-5 14-15 0.0 0.0 0.0 1.535912711008808E-4 5.1197090366960264E-5 16-17 0.0 0.0 0.0 2.0478836146784106E-4 5.1197090366960264E-5 18-19 0.0 0.0 0.0 2.559854518348013E-4 5.1197090366960264E-5 20-21 0.0 0.0 0.0 3.5837963256872183E-4 5.1197090366960264E-5 22-23 0.0 0.0 0.0 6.911607199539635E-4 5.1197090366960264E-5 24-25 0.0 0.0 0.0 0.0012543287139905265 5.1197090366960264E-5 26-27 0.0 0.0 0.0 0.0017918981628436094 5.1197090366960264E-5 28-29 0.0 0.0 0.0 0.002636650153898454 5.1197090366960264E-5 30-31 0.0 0.0 0.0 0.005042913401145586 5.1197090366960264E-5 32-33 0.0 0.0 0.0 0.008754702452750205 5.1197090366960264E-5 34-35 0.0 0.0 0.0 0.012748075501373106 5.1197090366960264E-5 36-37 0.0 0.0 0.0 0.016178280555959445 5.1197090366960264E-5 38-39 0.0 0.0 0.0 0.019941266697931025 5.1197090366960264E-5 40-41 0.0 0.0 0.0 0.026596888445635857 5.1197090366960264E-5 42-43 0.0 0.0 0.0 0.03665711670274355 5.1197090366960264E-5 44-45 0.0 0.0 0.0 0.04661495077911732 5.1197090366960264E-5 46-47 0.0 0.0 0.0 0.05713595284952766 5.1197090366960264E-5 48-49 0.0 0.0 0.0 0.06637702766076398 5.1197090366960264E-5 50-51 0.0 0.0 0.0 0.07851073807773357 5.1197090366960264E-5 52-53 0.0 0.0 0.0 0.10874261993942361 5.1197090366960264E-5 54-55 0.0 0.0 0.0 0.1486763504256526 5.1197090366960264E-5 56-57 0.0 0.0 0.0 0.18325998496853427 5.1197090366960264E-5 58-59 0.0 0.0 0.0 0.24536205558365706 5.1197090366960264E-5 60-61 0.0 0.0 0.0 0.31839470499212585 5.1197090366960264E-5 62-63 0.0 0.0 0.0 0.3658288092171146 5.1197090366960264E-5 64-65 0.0 0.0 0.0 0.4029466997331608 5.1197090366960264E-5 66-67 0.0 0.0 0.0 0.5043425372049256 5.1197090366960264E-5 68-69 0.0 0.0 0.0 0.6189472239913661 5.1197090366960264E-5 70-71 0.0 0.0 0.0 0.6673028758429601 5.1197090366960264E-5 72-73 0.0 0.0 0.0 0.764116573726882 5.1197090366960264E-5 74-75 0.0 0.0 0.0 0.9074684267543707 5.1197090366960264E-5 76-77 0.0 0.0 0.0 1.0512042579596117 5.1197090366960264E-5 78-79 0.0 0.0 0.0 1.1874653139712765 5.1197090366960264E-5 80-81 0.0 0.0 0.0 1.350323258428577 5.1197090366960264E-5 82-83 0.0 0.0 0.0 1.4981292583179913 5.1197090366960264E-5 84-85 0.0 0.0 0.0 1.6586833337087787 7.67956355504404E-5 86-87 0.0 0.0 0.0 1.9405489147240784 1.0239418073392053E-4 88-89 0.0 0.0 0.0 2.329262823335224 1.0239418073392053E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11230 0.0 33.30293 1 GGTATCA 7265 0.0 31.528177 1 ATCAACG 13540 0.0 27.339785 3 TCAACGC 13590 0.0 27.239199 4 CAACGCA 13630 0.0 27.128565 5 TATCAAC 13720 0.0 26.982483 2 GTACATG 8550 0.0 26.51183 1 AACGCAG 14015 0.0 26.280247 6 TACATGG 8740 0.0 25.392414 2 ACATGGG 8500 0.0 24.542675 3 ACGCAGA 15665 0.0 23.361643 7 CGCAGAG 15700 0.0 23.158794 8 GAGTACT 7770 0.0 23.118423 12-13 TAGACCG 155 3.890945E-5 21.46394 5 GCAGAGT 16385 0.0 21.349396 9 AGAGTAC 12385 0.0 21.277237 10-11 GTACTTT 8430 0.0 21.083223 14-15 CATGGGG 3260 0.0 20.844656 4 AGTACTT 8430 0.0 20.77291 12-13 CATGGGA 5630 0.0 20.257187 4 >>END_MODULE