FastQCFastQC Report
Thu 26 May 2016
SRR937050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953236
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116910.5985451834801324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94720.48493883995584763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61530.31501569702790655No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33800.1730461654403257No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24380.12481850631464912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83700.033.4964331
GTATCAA126250.032.5955661
ATCAACG155650.026.214293
TCAACGC156450.026.1079314
CAACGCA157800.025.8544795
TATCAAC159150.025.7571752
AACGCAG161400.025.3929066
GTACATG86450.024.3506621
GAGTACT99250.023.4960612-13
TACATGG89350.022.9127272
ACGCAGA179650.022.7075927
CGCAGAG179050.022.7041138
ACATGGG85550.022.5423973
AGTACTT105300.021.51463712-13
GTACTTT107550.021.46363414-15
GCAGAGT188050.021.415479
AGAGTAC148900.021.30553610-11
CATGGGA56850.019.8001044
ACTTTTT124250.018.80839216-17
TACTTTT121050.018.55981814-15
Produced by FastQC (version 0.11.5)