##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937050_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1953236 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19647293005044 33.0 31.0 34.0 31.0 34.0 2 32.25987028705185 33.0 31.0 34.0 30.0 34.0 3 32.39660952388754 34.0 31.0 34.0 31.0 34.0 4 36.07733115711568 37.0 35.0 37.0 35.0 37.0 5 35.99621704699278 37.0 35.0 37.0 35.0 37.0 6 36.037049286414955 37.0 35.0 37.0 35.0 37.0 7 35.9882103340303 37.0 35.0 37.0 35.0 37.0 8 35.975889754233485 37.0 35.0 37.0 35.0 37.0 9 37.669068151518815 39.0 37.0 39.0 35.0 39.0 10-11 37.5641138602811 39.0 37.0 39.0 35.0 39.0 12-13 37.492986510590626 39.0 37.0 39.0 35.0 39.0 14-15 38.69992412591208 40.0 38.0 41.0 34.5 41.0 16-17 38.63043021938977 40.0 38.0 41.0 34.0 41.0 18-19 38.6895119176587 40.0 38.0 41.0 34.5 41.0 20-21 38.79160403555945 40.0 38.0 41.0 35.0 41.0 22-23 38.72444599628514 40.0 38.0 41.0 34.0 41.0 24-25 38.64384206516775 40.0 38.0 41.0 34.0 41.0 26-27 38.46966725987029 40.0 38.0 41.0 34.0 41.0 28-29 38.3165702966769 40.0 38.0 41.0 34.0 41.0 30-31 38.24465527975114 40.0 38.0 41.0 34.0 41.0 32-33 38.14035682324102 40.0 37.0 41.0 34.0 41.0 34-35 38.012511032972974 40.0 37.0 41.0 33.0 41.0 36-37 37.837071147572544 40.0 37.0 41.0 33.0 41.0 38-39 37.68894951762101 40.0 37.0 41.0 33.0 41.0 40-41 37.46139304211063 40.0 37.0 41.0 33.0 41.0 42-43 37.21706875154871 40.0 36.5 41.0 32.0 41.0 44-45 36.93716529902173 40.0 36.0 41.0 31.0 41.0 46-47 36.52625361195473 39.5 36.0 41.0 30.5 41.0 48-49 36.121458697259314 39.0 36.0 41.0 29.5 41.0 50-51 35.86730763717236 39.0 35.0 41.0 29.0 41.0 52-53 35.65103858417518 39.0 35.0 40.0 28.0 41.0 54-55 35.39434789242058 39.0 35.0 40.0 27.5 41.0 56-57 35.15325695410078 38.0 35.0 40.0 27.0 41.0 58-59 34.825408962357855 38.0 34.0 40.0 26.0 41.0 60-61 34.510722462621004 38.0 34.0 40.0 26.0 41.0 62-63 34.1880269460526 37.5 34.0 40.0 25.5 41.0 64-65 33.79334192079196 37.0 33.5 39.5 24.5 41.0 66-67 33.36383749838729 36.0 33.0 39.0 23.0 40.0 68-69 32.93037195710093 36.0 32.0 39.0 22.0 40.0 70-71 32.53499756301849 35.0 32.0 38.5 22.0 40.0 72-73 32.14102904103754 35.0 31.5 38.0 20.5 40.0 74-75 31.660758351781354 35.0 31.0 37.0 18.5 39.0 76-77 30.20900751368498 33.5 29.5 35.5 17.5 38.5 78-79 30.899258205357675 34.0 31.0 36.0 17.5 39.0 80-81 30.587939706210616 34.0 31.0 36.0 11.5 37.5 82-83 30.384364971769926 34.0 30.5 35.5 12.0 37.0 84-85 30.03964779473653 34.0 30.0 35.0 7.5 37.0 86-87 29.672064717217992 34.0 30.0 35.0 4.5 36.0 88-89 29.2860158731459 34.0 29.5 35.0 2.0 36.0 90-91 28.83493520496243 34.0 29.0 35.0 2.0 35.5 92-93 28.404829216745952 34.0 29.0 35.0 2.0 35.0 94-95 27.916844917869625 33.5 28.5 35.0 2.0 35.0 96-97 27.286887759594848 33.0 27.0 35.0 2.0 35.0 98-99 26.51826660987203 33.0 25.5 34.5 2.0 35.0 100-101 23.278420784789958 29.0 12.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.27567594834159337 1101 2 0.26756035279444035 1101 3 0.22487250983774487 1101 4 0.16608064955703838 1101 5 0.240432283488623 1101 6 0.22480097521982145 1101 7 0.2333594612942136 1101 8 0.22836631564932475 1101 9 0.3678161414956591 1101 10-11 0.3084553127769567 1101 12-13 0.291924604970184 1101 14-15 0.3075082779748044 1101 16-17 0.3905019751839518 1101 18-19 0.3573950461180644 1101 20-21 0.3595026183606933 1101 22-23 0.4230170301946501 1101 24-25 0.3697863112316142 1101 26-27 0.2943983758263258 1101 28-29 0.2861717300660729 1101 30-31 0.27960205130527527 1101 32-33 0.37787505213351835 1101 34-35 0.467104703282331 1101 36-37 0.39233131760136075 1101 38-39 0.33130007443755005 1101 40-41 0.36664777847767027 1101 42-43 0.38787801089456764 1101 44-45 0.3721389291066899 1101 46-47 0.6049479212868292 1101 48-49 0.6906001215226567 1101 50-51 0.4741263248219667 1101 52-53 0.4341587640118121 1101 54-55 0.5612241836883953 1101 56-57 0.5483899573617421 1101 58-59 0.5527806999376352 1101 60-61 0.575585934305785 1101 62-63 0.5287848623631888 1101 64-65 0.6003891468283982 1101 66-67 0.48167298642515277 1101 68-69 0.5159079518189458 1101 70-71 0.5290304941034947 1101 72-73 0.493044232058665 1101 74-75 0.5563744756023787 1101 76-77 0.4718483788529788 1101 78-79 0.7383596075574772 1101 80-81 0.8669575650787529 1101 82-83 0.7729508195646808 1101 84-85 0.5832596112052713 1101 86-87 0.6584700279031566 1101 88-89 0.6079100231616401 1101 90-91 0.5164918798234268 1101 92-93 0.5549752253271798 1101 94-95 0.6314999063840574 1101 96-97 0.6315717250857311 1101 98-99 0.6762613704178833 1101 100-101 0.7952559951727416 1102 1 0.23309655530201212 1102 2 0.20936080910520616 1102 3 0.15592628153137866 1102 4 0.15623463346873478 1102 5 0.19535429545000227 1102 6 0.18911814475101352 1102 7 0.21801559255066394 1102 8 0.15854458301051721 1102 9 0.2808675519488659 1102 10-11 0.32475935288869096 1102 12-13 0.2673520293308016 1102 14-15 0.2837485583039481 1102 16-17 0.34505805778961474 1102 18-19 0.3237059539583882 1102 20-21 0.24845590312712318 1102 22-23 0.2990458788487942 1102 24-25 0.32112530860614896 1102 26-27 0.3277697700066895 1102 28-29 0.26164240538922456 1102 30-31 0.35734369050103254 1102 32-33 0.3106905797009034 1102 34-35 0.31687916917226033 1102 36-37 0.4393668704168121 1102 38-39 0.509949092614491 1102 40-41 0.5700856271066357 1102 42-43 0.48934817664274277 1102 44-45 0.6342515577586383 1102 46-47 0.8261522168151458 1102 48-49 0.9364063509194622 1102 50-51 0.7121652056023962 1102 52-53 0.6344537093635623 1102 54-55 0.6595401746963887 1102 56-57 0.6612010497395104 1102 58-59 0.7432060377669742 1102 60-61 0.5983789294323145 1102 62-63 0.6023728363937408 1102 64-65 0.6651114599213273 1102 66-67 0.7455544483810783 1102 68-69 0.7281561525789115 1102 70-71 0.7220841798194542 1102 72-73 0.7478650105550244 1102 74-75 0.8587823222182145 1102 76-77 0.6936380040742165 1102 78-79 0.7416846891314108 1102 80-81 0.7455374341410526 1102 82-83 0.8203292345558317 1102 84-85 0.676218249654486 1102 86-87 0.6643309265166089 1102 88-89 0.8417231538393146 1102 90-91 0.687735808961051 1102 92-93 0.7229627501768228 1102 94-95 0.8279837866192565 1102 96-97 1.0512609787156677 1102 98-99 1.2717956692175498 1102 100-101 1.3812645971338675 1103 1 0.2071773122660403 1103 2 0.12567489571545565 1103 3 0.19720695369184682 1103 4 0.11172082523540894 1103 5 0.1651418717271227 1103 6 0.20311073365649435 1103 7 0.23897221368149957 1103 8 0.1923980295381824 1103 9 0.1260595582906987 1103 10-11 0.21827729529600504 1103 12-13 0.235189469184796 1103 14-15 0.36982302874253037 1103 16-17 0.28127217758387957 1103 18-19 0.27517780286054716 1103 20-21 0.3081496654559004 1103 22-23 0.28526817942213256 1103 24-25 0.37158631279101684 1103 26-27 0.3838081067505996 1103 28-29 0.30812651393779333 1103 30-31 0.33115828408914183 1103 32-33 0.3506191188672432 1103 34-35 0.33259663502038705 1103 36-37 0.4203402218169927 1103 38-39 0.16095014344132608 1103 40-41 0.3151817685442424 1103 42-43 0.14707071687834627 1103 44-45 0.3438206496230336 1103 46-47 0.2502587835610939 1103 48-49 0.31553649213584123 1103 50-51 0.2786835812453319 1103 52-53 0.22719032975697928 1103 54-55 0.0856723484330928 1103 56-57 0.14599632286230957 1103 58-59 0.21445002316600892 1103 60-61 0.2011583203214613 1103 62-63 0.22082475645716215 1103 64-65 0.1582626499366313 1103 66-67 0.014082760790053328 1103 68-69 0.2869050925342904 1103 70-71 0.31025511892681124 1103 72-73 0.3551709071684144 1103 74-75 0.2198663561027736 1103 76-77 0.30032861392248833 1103 78-79 0.34355555265297255 1103 80-81 0.6951075399611604 1103 82-83 0.557941691462716 1103 84-85 0.49256782471486105 1103 86-87 0.47789846057630214 1103 88-89 0.20462251412542898 1103 90-91 0.23400329969718214 1103 92-93 0.07074668082225344 1103 94-95 0.40842446560088064 1103 96-97 0.440974282736466 1103 98-99 0.6287414963288818 1103 100-101 0.9025334705368913 1104 1 0.11612367806821311 1104 2 0.055398135953041105 1104 3 0.0328988062925788 1104 4 0.053239938181292246 1104 5 -0.019753120573398064 1104 6 0.06411021411761908 1104 7 0.10031618117989183 1104 8 0.06421983238487883 1104 9 0.19709362787146745 1104 10-11 0.08137856793221232 1104 12-13 0.18078321786839524 1104 14-15 0.07570645545244759 1104 16-17 0.1209810953984487 1104 18-19 0.1300165351856961 1104 20-21 0.03721973767648734 1104 22-23 0.06877399030062037 1104 24-25 0.14771958621356163 1104 26-27 0.19882956698247511 1104 28-29 0.1745036897993657 1104 30-31 0.2202098214093553 1104 32-33 0.1535739377073142 1104 34-35 0.19976177817441254 1104 36-37 0.1260464904513654 1104 38-39 0.1961903248586836 1104 40-41 0.11284407763710647 1104 42-43 0.06090870499916434 1104 44-45 -0.04254847572625664 1104 46-47 -0.18029759108728882 1104 48-49 -0.24101227197010644 1104 50-51 -0.2938847661997954 1104 52-53 -0.1484156819162621 1104 54-55 -0.2680835431845452 1104 56-57 -0.42046861294421944 1104 58-59 -0.38973296685041703 1104 60-61 -0.2203016775254909 1104 62-63 -0.24786904792475895 1104 64-65 -0.17493471398864102 1104 66-67 -0.3433538999323389 1104 68-69 -0.152524163589014 1104 70-71 -0.2113211901005272 1104 72-73 -0.2766496787403838 1104 74-75 -0.18482096106400903 1104 76-77 -0.09765173721090292 1104 78-79 -7.13137875038683E-4 1104 80-81 0.02212730584102829 1104 82-83 -0.04501874401383077 1104 84-85 -0.203398260161876 1104 86-87 -0.07281461822978486 1104 88-89 -0.05760186114077115 1104 90-91 0.011529030913042249 1104 92-93 -0.007714922446627526 1104 94-95 0.196179964053961 1104 96-97 0.3909518161905545 1104 98-99 0.6265615392961514 1104 100-101 1.0429627219796629 1105 1 0.15860836013803237 1105 2 0.19257762692188862 1105 3 0.15265176864579644 1105 4 0.14416179235281845 1105 5 0.04950786503528093 1105 6 0.10667056218119342 1105 7 0.13430972001339825 1105 8 0.05315988777475411 1105 9 0.03179889995869445 1105 10-11 0.15093719282100437 1105 12-13 0.17903950095379173 1105 14-15 0.21725491863472968 1105 16-17 0.2976158207693089 1105 18-19 0.32670348782647096 1105 20-21 0.269606113068491 1105 22-23 0.12505515449287685 1105 24-25 0.10573131444259332 1105 26-27 0.13644251177881017 1105 28-29 0.23685084301413895 1105 30-31 0.15884869067566143 1105 32-33 0.045727285484183255 1105 34-35 -0.01553324530864586 1105 36-37 0.02706177439095825 1105 38-39 0.17084916285365637 1105 40-41 0.2855054516251556 1105 42-43 0.2977983893005387 1105 44-45 0.2610697479554247 1105 46-47 0.1771930467238363 1105 48-49 0.27663711664275326 1105 50-51 0.08756406023837826 1105 52-53 0.29169557074017405 1105 54-55 0.1573638763390548 1105 56-57 0.14252170121744 1105 58-59 0.30500558645125153 1105 60-61 0.2671355507345723 1105 62-63 0.2991091892349331 1105 64-65 0.19482534205148028 1105 66-67 0.037928963789070735 1105 68-69 0.09301937539227367 1105 70-71 0.19497770830900407 1105 72-73 0.18693872189923155 1105 74-75 0.14538247996528497 1105 76-77 0.11319657907295522 1105 78-79 0.2230384471206328 1105 80-81 0.39881124366486276 1105 82-83 0.43158287748476454 1105 84-85 0.13130302172646324 1105 86-87 0.08429163042167431 1105 88-89 -0.009519225599628811 1105 90-91 0.09869093544975271 1105 92-93 0.24648838262033124 1105 94-95 0.43017631080520147 1105 96-97 0.6181394057852962 1105 98-99 0.7133247195142225 1105 100-101 0.8621833622440214 1106 1 0.12148902986832155 1106 2 0.09555652914487212 1106 3 0.12367992468030309 1106 4 0.06388703156054021 1106 5 0.10693345920908826 1106 6 0.10799967456750181 1106 7 0.03742824063328953 1106 8 0.08300229350923871 1106 9 0.14082923308203021 1106 10-11 0.15700323574476727 1106 12-13 0.0479049748641458 1106 14-15 0.1475678862750911 1106 16-17 0.14084660416197892 1106 18-19 0.21530237957122011 1106 20-21 0.22272393217437525 1106 22-23 0.22311705951062066 1106 24-25 0.024972166785531158 1106 26-27 0.14529835612619735 1106 28-29 0.13040054151710478 1106 30-31 -0.04358275686255553 1106 32-33 0.06910943164479022 1106 34-35 0.10775339228682412 1106 36-37 0.03985927193651406 1106 38-39 0.05652753202864602 1106 40-41 0.10947060725347058 1106 42-43 0.06945575206643184 1106 44-45 0.07980109581021821 1106 46-47 0.12831907936100606 1106 48-49 -0.02356912847444903 1106 50-51 -0.14323935124133413 1106 52-53 -0.17551720461690223 1106 54-55 0.13409653893374696 1106 56-57 0.08603853122280469 1106 58-59 0.1858606190955996 1106 60-61 -0.02922947745773996 1106 62-63 0.01949374894592637 1106 64-65 0.03785935932104678 1106 66-67 0.03324627226120214 1106 68-69 0.0839917456643704 1106 70-71 0.1443200132650091 1106 72-73 0.11440713580344664 1106 74-75 0.15973271887357754 1106 76-77 -0.025268597258580883 1106 78-79 0.08844939231322613 1106 80-81 0.5145880882959375 1106 82-83 0.39911424286029273 1106 84-85 0.19929016588348247 1106 86-87 0.24715734592660255 1106 88-89 0.08840442982901209 1106 90-91 0.26908612053138725 1106 92-93 0.2805278953110637 1106 94-95 0.4385617985678891 1106 96-97 0.708120040576766 1106 98-99 0.6760625199290402 1106 100-101 0.6458884580759658 1107 1 0.120963731461174 1107 2 0.04290405904116312 1107 3 -0.018630047276289474 1107 4 0.0866864166924799 1107 5 0.011533172858705143 1107 6 0.0817831605481274 1107 7 0.02896968129913091 1107 8 0.04392833902338822 1107 9 -0.04077449399084543 1107 10-11 0.09856620826681706 1107 12-13 0.18883877256365622 1107 14-15 0.12992772034533573 1107 16-17 0.2412365082493011 1107 18-19 0.23272489626334192 1107 20-21 0.14347002802394826 1107 22-23 0.17583105944822108 1107 24-25 0.1729268555847696 1107 26-27 0.12603102011342315 1107 28-29 0.052311078716300585 1107 30-31 0.1335476867438814 1107 32-33 0.10992224935620953 1107 34-35 0.0392110935738188 1107 36-37 0.06273556936083224 1107 38-39 -0.053395910658785795 1107 40-41 0.17951977733576996 1107 42-43 0.041888701220770486 1107 44-45 -0.05314081550866234 1107 46-47 -0.06462177060409147 1107 48-49 -0.15598986488902966 1107 50-51 -0.1702859443136333 1107 52-53 -0.16996983829817935 1107 54-55 -0.1914172584860907 1107 56-57 -0.32031205759547987 1107 58-59 -0.2636162518183198 1107 60-61 -0.2586518535921911 1107 62-63 -0.19491710468729195 1107 64-65 -0.2723689715818125 1107 66-67 -0.06021309922373774 1107 68-69 -0.09618901435816696 1107 70-71 -0.13568931660839922 1107 72-73 -0.051713555180896265 1107 74-75 -0.0941972482710689 1107 76-77 -0.022916766369526442 1107 78-79 0.16175182686516365 1107 80-81 0.20634262441800288 1107 82-83 0.1637794807631039 1107 84-85 0.014966719753822133 1107 86-87 -0.16613740098175356 1107 88-89 -0.24463440173521533 1107 90-91 -0.2722003621601168 1107 92-93 -0.16206254655274677 1107 94-95 -0.0599661109129741 1107 96-97 0.015405266137751283 1107 98-99 0.13290737331341518 1107 100-101 0.44799850827154586 1108 1 0.18230123717475522 1108 2 0.027337999981554617 1108 3 -0.017067916147688322 1108 4 0.02735808284848673 1108 5 0.03436489969170253 1108 6 0.10688525159375217 1108 7 0.15989234325093804 1108 8 0.14966603158995184 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-1.3824513655498336 2316 54-55 -1.1021741736196233 2316 56-57 -1.1553132860561632 2316 58-59 -1.2317193618407245 2316 60-61 -0.9945768111461248 2316 62-63 -1.0457819776791268 2316 64-65 -0.9672807607706275 2316 66-67 -1.2032334256642443 2316 68-69 -1.365218237054247 2316 70-71 -1.092511673859434 2316 72-73 -1.0203612216556515 2316 74-75 -0.93318822366885 2316 76-77 -0.9729908407152337 2316 78-79 -0.8844538797930923 2316 80-81 -0.7206015521283007 2316 82-83 -0.8483850302912117 2316 84-85 -1.2109838704573619 2316 86-87 -0.9652950803278486 2316 88-89 -1.019321877670226 2316 90-91 -1.0059617620919106 2316 92-93 -0.7995621340099781 2316 94-95 -1.039007104829345 2316 96-97 -0.86110051496156 2316 98-99 -1.039254057421573 2316 100-101 -1.4640647922906105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 11.0 5 39.0 6 78.0 7 196.0 8 487.0 9 1012.0 10 1771.0 11 2932.0 12 4604.0 13 6693.0 14 8603.0 15 11871.0 16 16206.0 17 23262.0 18 21014.0 19 12008.0 20 9857.0 21 9818.0 22 10291.0 23 11171.0 24 12568.0 25 14911.0 26 18233.0 27 22473.0 28 28401.0 29 35415.0 30 44385.0 31 55795.0 32 71294.0 33 94000.0 34 128375.0 35 183384.0 36 283218.0 37 439578.0 38 342346.0 39 26930.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.569428441207954 20.308337558187766 15.387534374951992 21.734699625652294 2 15.165837466533748 21.570570077011418 40.55139376791715 22.712198688537686 3 19.00951407602966 26.269776615577772 30.587849161367558 24.132860147025013 4 12.675254488588394 17.995567344569075 38.398786421063356 30.930391745779172 5 12.981059198069978 38.319002548719034 34.55623719368524 14.143701059525748 6 30.0576069660809 38.09355346716936 17.679328048428353 14.16951151832139 7 27.145670057279304 33.84439975507312 21.662103299345294 17.347826888302283 8 25.777046225994894 35.310127327554866 20.18446368323239 18.72836276321785 9 26.395120712499665 17.138789168333986 21.230563024642183 35.235527094524166 10-11 24.109708197063746 27.74439443057572 27.804986187025023 20.340911185335514 12-13 25.471436331456616 25.257995333890015 28.482751417777603 20.787816916875766 14-15 22.447031490306344 26.348352170449452 27.3246550852022 23.87996125404201 16-17 20.89943629400361 29.78085836595657 27.84611883519362 21.473586504846192 18-19 20.75813337183571 27.865201733016715 31.012489721605263 20.36417517354231 20-21 21.691238209141066 26.852414079811265 31.328563061384145 20.12778464966353 22-23 21.41300385616485 26.79520037517228 31.164769643811603 20.627026124851273 24-25 21.247916163200774 26.974396967326257 31.324547085495674 20.453139783977292 26-27 21.04821489508712 27.12354409264559 31.424379601770603 20.40386141049669 28-29 20.758116043206375 27.175019705938 31.531651230881913 20.535213019973718 30-31 21.00741501742684 26.97087434826998 31.42961546232276 20.592095171980418 32-33 20.94356353575603 27.235804616267572 31.31268165488528 20.507950193091112 34-35 21.280459114528266 27.080983497947674 31.116385778672512 20.522171608851544 36-37 21.337868422669644 26.83497019847203 31.11023493025828 20.716926448600045 38-39 20.89096777216072 27.379570252808577 31.118360065916452 20.61110190911425 40-41 21.126419814150175 27.30234095406186 31.10453841303391 20.466700818754052 42-43 20.972910423784246 27.433268093745323 30.65808354336726 20.935737939103173 44-45 21.191187180888576 27.344954567621222 30.425319767404652 21.03853848408555 46-47 21.595486973748848 27.623521617579033 29.861168175864055 20.919823232808067 48-49 21.55144100448315 28.00588878731786 29.5205799088523 20.92209029934669 50-51 21.522898906893992 28.12615518521363 29.284745155262016 21.066200752630365 52-53 21.607841581218295 28.32822413733519 29.064460347305864 20.99947393414065 54-55 21.61417161580995 28.197419619086627 28.98612533278722 21.2022834323162 56-57 21.64313631527804 28.476998518345585 28.83509913363663 21.04476603273975 58-59 21.79193515521565 28.005040911971935 29.050981508464492 21.152042424347922 60-61 21.67616244943326 28.201002900059922 29.35045466867667 20.77237998183015 62-63 21.448893016512084 28.231201964329962 29.448796765982195 20.871108253175755 64-65 21.549624654357647 28.163429151029934 29.244421528913634 21.042524665698785 66-67 21.527598586126675 28.39099720665815 28.82479459517022 21.256609612044954 68-69 21.480628564963027 28.232587262717644 29.08349379560119 21.20329037671813 70-71 21.626956038461962 28.16144992410979 29.075332392460286 21.136261644967963 72-73 21.613490583862482 28.15296851934252 28.978016638188777 21.255524258606222 74-75 21.58089742122662 28.68766027253339 28.594271161734426 21.13717114450557 76-77 21.56789760952881 28.31632790202291 28.812526223657624 21.303248264790657 78-79 21.572500330261473 28.30297317252757 28.589660614091773 21.534865883119185 80-81 21.69081592609592 28.63842001562024 28.262914667952053 21.407849390331783 82-83 21.721424157261662 28.528354786964698 28.496482819601592 21.253738236172044 84-85 21.48210400639151 28.528762684525756 28.364904760941762 21.624228548140977 86-87 21.351644143360044 28.601996891312677 28.538742886164293 21.50761607916299 88-89 21.584192581492154 28.49573996030636 28.307818490540825 21.612248967660662 90-91 21.449233291300157 28.397788471236456 28.442919099189595 21.71005913827379 92-93 21.470602465091858 28.396835299005467 28.483486485358767 21.649075750543904 94-95 21.368414262214593 28.15836642830776 28.565233663233524 21.907985646244118 96-97 21.380722151982496 28.276269351584798 28.561181815877944 21.781826680554765 98-99 21.242423769237682 28.420890417183227 28.29131021658734 22.045375596991754 100-101 20.848984878776786 28.79821217838282 28.2725148025179 22.080288140322494 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 721.0 1 536.0 2 392.0 3 432.5 4 390.5 5 399.5 6 687.0 7 1451.5 8 2470.0 9 3046.5 10 2891.5 11 2655.0 12 2748.5 13 2992.0 14 3317.0 15 3611.0 16 3862.5 17 4073.0 18 4291.0 19 4572.5 20 4917.5 21 5498.5 22 6477.0 23 7737.5 24 9063.0 25 11126.0 26 14358.0 27 17658.0 28 20149.5 29 23485.5 30 28166.0 31 32433.5 32 37384.5 33 43059.0 34 49306.5 35 54872.5 36 62154.5 37 68659.5 38 69472.5 39 68997.5 40 68227.0 41 67462.5 42 66374.5 43 67902.0 44 70722.5 45 71402.0 46 70068.0 47 70001.0 48 74467.0 49 72358.5 50 65175.0 51 60504.5 52 56326.0 53 53814.0 54 51253.5 55 46641.0 56 41031.5 57 35814.0 58 31987.0 59 28634.0 60 24522.5 61 19935.0 62 15402.5 63 11698.0 64 8683.5 65 6316.5 66 4688.0 67 3694.5 68 2701.5 69 1679.5 70 1102.5 71 753.5 72 543.5 73 394.0 74 274.5 75 199.5 76 142.0 77 76.0 78 42.5 79 26.0 80 13.5 81 7.0 82 4.5 83 3.5 84 3.0 85 3.5 86 1.5 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025905727725681893 2 0.007628366464677079 3 0.012031316236235661 4 2.559854518348013E-4 5 0.005017314855962106 6 0.0 7 0.0 8 3.071825422017616E-4 9 0.0 10-11 0.0 12-13 1.535912711008808E-4 14-15 0.0 16-17 7.67956355504404E-5 18-19 0.005631679940365629 20-21 0.0014335185302748875 22-23 0.0 24-25 0.005529285759631708 26-27 0.00591326393738391 28-29 0.007397979558025759 30-31 0.03299652474150589 32-33 6.655621747704834E-4 34-35 0.004351752681191623 36-37 0.016178280555959445 38-39 0.008063541732796243 40-41 0.010623396251144255 42-43 0.009138680630502407 44-45 0.002995029786467176 46-47 0.0023294676116966924 48-49 0.0016639054369262088 50-51 0.004402949771558583 52-53 0.002995029786467176 54-55 0.0018430952532105696 56-57 7.167592651374438E-4 58-59 9.215476266052848E-4 60-61 2.5598545183480132E-5 62-63 0.0 64-65 0.008677906817199765 66-67 0.0010239418073392053 68-69 0.0012799272591740066 70-71 0.0043773512263751025 72-73 0.01326004640504271 74-75 0.006015658118117831 76-77 0.0043773512263751025 78-79 0.012184907507336543 80-81 7.67956355504404E-5 82-83 0.005580482849998669 84-85 9.983432621557251E-4 86-87 0.0 88-89 0.0013567228947244472 90-91 8.703505362383246E-4 92-93 1.2799272591740066E-4 94-95 9.727447169722451E-4 96-97 2.303869066513212E-4 98-99 2.8158399701828143E-4 100-101 0.0010751388977061655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1953236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.62342922110428 #Duplication Level Percentage of deduplicated Percentage of total 1 77.03132406660824 41.306837538937124 2 13.023353815001363 13.967137830402477 3 4.248519240977914 6.834605124392365 4 1.8553622548361082 3.979635462068499 5 0.9937347354631322 2.6643732125830026 6 0.6074388706018783 1.9543773170320404 7 0.38664453726450987 1.4513244184411043 8 0.28353578289550835 1.2163328788598144 9 0.2023041031369264 0.9763415780131757 >10 1.186995196256316 11.923657711348087 >50 0.1102704694640943 4.085236510009126 >100 0.06658954066764683 6.526291749741726 >500 0.0029694876005177634 1.0891612998780875 >1k 6.705294581814303E-4 0.6215780461151073 >5k 1.915798451946944E-4 0.8025912710146724 >10k+ 9.57899225973472E-5 0.6005180511636823 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11691 0.5985451834801324 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9472 0.48493883995584763 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6153 0.31501569702790655 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3380 0.1730461654403257 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2438 0.12481850631464912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 5.1197090366960264E-5 9 0.0 0.0 0.0 1.0239418073392053E-4 5.1197090366960264E-5 10-11 0.0 0.0 0.0 1.7918981628436091E-4 5.1197090366960264E-5 12-13 0.0 0.0 0.0 2.559854518348013E-4 5.1197090366960264E-5 14-15 0.0 0.0 0.0 2.559854518348013E-4 1.0239418073392053E-4 16-17 0.0 0.0 0.0 3.071825422017616E-4 1.0239418073392053E-4 18-19 0.0 0.0 0.0 3.8397817775220194E-4 1.0239418073392053E-4 20-21 0.0 0.0 0.0 5.119709036696027E-4 1.0239418073392053E-4 22-23 0.0 0.0 0.0 8.447519910548443E-4 1.0239418073392053E-4 24-25 0.0 0.0 0.0 0.0014079199850914072 1.0239418073392053E-4 26-27 0.0 0.0 0.0 0.00194548943394449 1.0239418073392053E-4 28-29 0.0 0.0 0.0 0.0027902414249993345 1.0239418073392053E-4 30-31 0.0 0.0 0.0 0.005222103217429947 1.0239418073392053E-4 32-33 0.0 0.0 0.0 0.009010687904585007 1.0239418073392053E-4 34-35 0.0 0.0 0.0 0.013106455133941828 1.0239418073392053E-4 36-37 0.0 0.0 0.0 0.016485463098161204 1.0239418073392053E-4 38-39 0.0 0.0 0.0 0.020171653604582347 1.0239418073392053E-4 40-41 0.0 0.0 0.0 0.02685287389747066 1.0239418073392053E-4 42-43 0.0 0.0 0.0 0.03706669342567923 1.0239418073392053E-4 44-45 0.0 0.0 0.0 0.04707572459241996 1.0239418073392053E-4 46-47 0.0 0.0 0.0 0.05754552957246334 1.0239418073392053E-4 48-49 0.0 0.0 0.0 0.06688899856443359 1.0239418073392053E-4 50-51 0.0 0.0 0.0 0.07938108861397189 1.0239418073392053E-4 52-53 0.0 0.0 0.0 0.10979216029194629 1.0239418073392053E-4 54-55 0.0 0.0 0.0 0.14972589077817527 1.0239418073392053E-4 56-57 0.0 0.0 0.0 0.1846167078632587 1.0239418073392053E-4 58-59 0.0 2.5598545183480132E-5 0.0 0.2472563479272346 1.0239418073392053E-4 60-61 0.0 5.1197090366960264E-5 0.0 0.3202633987905199 1.0239418073392053E-4 62-63 0.0 5.1197090366960264E-5 0.0 0.3678510942866095 1.0239418073392053E-4 64-65 0.0 5.1197090366960264E-5 0.0 0.4044058168086191 1.0239418073392053E-4 66-67 0.0 5.1197090366960264E-5 0.0 0.5055456688285491 1.0239418073392053E-4 68-69 0.0 5.1197090366960264E-5 0.0 0.61963838471132 1.2799272591740066E-4 70-71 0.0 5.1197090366960264E-5 0.0 0.6677892482014463 1.535912711008808E-4 72-73 0.0 5.1197090366960264E-5 0.0 0.7637581940943132 1.535912711008808E-4 74-75 0.0 5.1197090366960264E-5 0.0 0.9063420907662976 1.535912711008808E-4 76-77 0.0 5.1197090366960264E-5 0.0 1.048388417989429 1.535912711008808E-4 78-79 0.0 5.1197090366960264E-5 0.0 1.1843166929137083 1.535912711008808E-4 80-81 0.0 5.1197090366960264E-5 0.0 1.345894710111835 1.535912711008808E-4 82-83 0.0 5.1197090366960264E-5 0.0 1.49385430127235 1.7918981628436091E-4 84-85 0.0 5.1197090366960264E-5 0.0 1.654485172298688 2.0478836146784106E-4 86-87 0.0 5.1197090366960264E-5 0.0 1.9366835344013729 2.0478836146784106E-4 88-89 0.0 5.1197090366960264E-5 0.0 2.3250902604703167 2.0478836146784106E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8370 0.0 33.496433 1 GTATCAA 12625 0.0 32.595566 1 ATCAACG 15565 0.0 26.21429 3 TCAACGC 15645 0.0 26.107931 4 CAACGCA 15780 0.0 25.854479 5 TATCAAC 15915 0.0 25.757175 2 AACGCAG 16140 0.0 25.392906 6 GTACATG 8645 0.0 24.350662 1 GAGTACT 9925 0.0 23.49606 12-13 TACATGG 8935 0.0 22.912727 2 ACGCAGA 17965 0.0 22.707592 7 CGCAGAG 17905 0.0 22.704113 8 ACATGGG 8555 0.0 22.542397 3 AGTACTT 10530 0.0 21.514637 12-13 GTACTTT 10755 0.0 21.463634 14-15 GCAGAGT 18805 0.0 21.41547 9 AGAGTAC 14890 0.0 21.305536 10-11 CATGGGA 5685 0.0 19.800104 4 ACTTTTT 12425 0.0 18.808392 16-17 TACTTTT 12105 0.0 18.559818 14-15 >>END_MODULE