FastQCFastQC Report
Thu 26 May 2016
SRR937049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994631
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96620.4844003728007837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71290.35740946571070037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45610.22866384810022508No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24550.12308040935892403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA121000.030.085321
GGTATCA83800.029.4330041
GTACATG88550.025.4927271
ATCAACG143900.025.1673853
TCAACGC144500.024.9971034
CAACGCA146750.024.6462295
TATCAAC146700.024.6222322
AACGCAG149650.024.1674086
TACATGG91700.023.9969232
ACATGGG89450.023.4847453
GAGTACT77650.022.45740512-13
ACGCAGA165750.021.5889197
CGCAGAG166300.021.3460668
GTACTTT84050.020.63173114-15
GCAGAGT172900.020.2818349
CATGGGA56300.020.1759244
AGAGTAC129700.020.16751110-11
AGTACTT83200.020.10269712-13
GTATAGG8350.019.3510111
CATGGGG34850.018.8200264
Produced by FastQC (version 0.11.5)