##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937049_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1994631 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79530349222488 33.0 31.0 34.0 30.0 34.0 2 31.919107343664066 33.0 31.0 34.0 30.0 34.0 3 31.92605599732482 33.0 31.0 34.0 30.0 34.0 4 35.4881584613896 37.0 35.0 37.0 33.0 37.0 5 35.4720637551507 37.0 35.0 37.0 33.0 37.0 6 35.58435219346335 37.0 35.0 37.0 33.0 37.0 7 35.53076132878713 37.0 35.0 37.0 33.0 37.0 8 35.49346871677017 37.0 35.0 37.0 33.0 37.0 9 37.10305114078744 39.0 37.0 39.0 33.0 39.0 10-11 36.98236816734524 39.0 37.0 39.0 33.0 39.0 12-13 36.88714077942236 39.0 37.0 39.0 33.0 39.0 14-15 38.068228409164405 40.0 38.0 41.0 33.0 41.0 16-17 37.99311526793677 40.0 38.0 41.0 33.0 41.0 18-19 37.95296849392193 40.0 38.0 41.0 33.0 41.0 20-21 37.84883870751031 40.0 38.0 41.0 32.5 41.0 22-23 37.7245044321481 40.0 37.5 41.0 32.0 41.0 24-25 37.85189766929322 40.0 38.0 41.0 33.0 41.0 26-27 37.80099426911544 40.0 38.0 41.0 33.0 41.0 28-29 37.720303905835216 40.0 37.5 41.0 32.0 41.0 30-31 37.539693557354724 40.0 37.0 41.0 32.0 41.0 32-33 37.33342934106609 40.0 37.0 41.0 31.5 41.0 34-35 37.14581293482354 40.0 36.0 41.0 31.0 41.0 36-37 36.960046244142404 39.5 36.0 41.0 31.0 41.0 38-39 36.80414497719127 39.0 36.0 41.0 30.0 41.0 40-41 36.63296970717892 39.0 36.0 41.0 30.0 41.0 42-43 36.406711065856285 39.0 35.0 41.0 30.0 41.0 44-45 36.12703753225534 39.0 35.0 41.0 29.5 41.0 46-47 35.769349067571895 39.0 35.0 40.0 28.0 41.0 48-49 35.50239392649568 39.0 35.0 40.0 27.0 41.0 50-51 34.79760040829607 38.0 34.0 40.0 26.0 40.5 52-53 34.99663772397 38.5 34.5 40.0 26.5 40.5 54-55 35.40883100683786 39.0 35.0 40.5 27.0 41.0 56-57 35.31219684242349 39.0 35.0 41.0 26.5 41.0 58-59 35.07597370140141 39.0 35.0 40.5 26.0 41.0 60-61 34.812243718261676 38.0 34.5 40.0 26.0 41.0 62-63 34.48412488324908 38.0 34.0 40.0 25.0 41.0 64-65 34.16812884187601 37.5 34.0 40.0 23.5 41.0 66-67 33.79678471857702 37.0 34.0 40.0 23.0 41.0 68-69 33.39966490042519 36.5 33.0 39.0 22.0 41.0 70-71 33.01230227545847 36.0 33.0 39.0 21.5 41.0 72-73 32.60316469562541 35.5 32.5 39.0 20.5 40.0 74-75 32.143626314842194 35.0 32.0 37.5 19.0 40.0 76-77 31.705038926999528 35.0 31.5 37.0 18.0 39.0 78-79 31.26035016000453 35.0 31.0 36.5 14.0 39.0 80-81 30.808653329864022 35.0 31.0 36.0 9.5 38.5 82-83 30.378355946538484 34.5 30.5 36.0 7.0 37.0 84-85 29.963662201179062 34.0 30.0 35.0 4.5 37.0 86-87 29.612190425196438 34.0 30.0 35.0 2.0 36.5 88-89 29.245321064397373 34.0 29.5 35.0 2.0 36.0 90-91 28.852292729833238 34.0 29.0 35.0 2.0 36.0 92-93 28.476653325853256 34.0 29.0 35.0 2.0 35.0 94-95 28.067613007117608 34.0 29.0 35.0 2.0 35.0 96-97 27.560019873350008 34.0 27.5 35.0 2.0 35.0 98-99 26.916883874761798 34.0 26.5 35.0 2.0 35.0 100-101 24.337877030889423 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3341366403954318 1101 2 0.3198759738401158 1101 3 0.32769251417945355 1101 4 0.2811681546867604 1101 5 0.315681661576086 1101 6 0.23546210600722617 1101 7 0.1871428656702534 1101 8 0.19815381782745334 1101 9 0.2535676051915772 1101 10-11 0.37348151963508514 1101 12-13 0.42674867706421793 1101 14-15 0.4965825515026765 1101 16-17 0.4250836618460241 1101 18-19 0.5001358971598222 1101 20-21 0.47657091708536115 1101 22-23 0.5072273014334598 1101 24-25 0.4582262871235443 1101 26-27 0.4547518594277875 1101 28-29 0.3929270999445009 1101 30-31 0.4556156881676898 1101 32-33 0.5320095626264774 1101 34-35 0.5242272015796487 1101 36-37 0.5182225964183047 1101 38-39 0.5454905685811013 1101 40-41 0.7010479222251575 1101 42-43 0.6865690150349053 1101 44-45 0.5467344475237326 1101 46-47 0.7058147675493558 1101 48-49 0.6212266573971803 1101 50-51 0.5544283770465768 1101 52-53 0.639954932892735 1101 54-55 0.6104733936837334 1101 56-57 0.4326010421621902 1101 58-59 0.44225841296045587 1101 60-61 0.6320845927029168 1101 62-63 0.6724661429242786 1101 64-65 0.6919473876047704 1101 66-67 0.7162898662506123 1101 68-69 0.5853801774724587 1101 70-71 0.5023417704939988 1101 72-73 0.6081533912004105 1101 74-75 0.654789004527899 1101 76-77 0.6147693569383712 1101 78-79 0.7544627281580603 1101 80-81 0.6741683687403821 1101 82-83 0.6083690436137026 1101 84-85 0.5487161810493077 1101 86-87 0.5740059124007146 1101 88-89 0.5084403208019985 1101 90-91 0.48025414064601435 1101 92-93 0.5970570513571367 1101 94-95 0.8220033346925497 1101 96-97 0.7063768944009645 1101 98-99 0.3640063365463959 1101 100-101 0.5016272498106957 1102 1 0.2792652838428431 1102 2 0.33218769630935796 1102 3 0.2879444116913561 1102 4 0.2655136814989376 1102 5 0.270689086012446 1102 6 0.2173697791411513 1102 7 0.2315624684278248 1102 8 0.39132880996793773 1102 9 0.41579610099234543 1102 10-11 0.4057916772183958 1102 12-13 0.42656569768983843 1102 14-15 0.3790845304167263 1102 16-17 0.5878630255620081 1102 18-19 0.5372280794669209 1102 20-21 0.5485177755242319 1102 22-23 0.46335874202674177 1102 24-25 0.2060850951155544 1102 26-27 0.3944055073663506 1102 28-29 0.1918661002701043 1102 30-31 0.2271447992563509 1102 32-33 0.4391160394296705 1102 34-35 0.32802752353914144 1102 36-37 0.41667658736862734 1102 38-39 0.34265345846927175 1102 40-41 0.33732703050684876 1102 42-43 0.49732255322093266 1102 44-45 0.29688918175978785 1102 46-47 0.48199888795715395 1102 48-49 0.45512524436833957 1102 50-51 0.32983531756126894 1102 52-53 0.5391845248154041 1102 54-55 0.6384123968163067 1102 56-57 0.7059129302426683 1102 58-59 0.5141472022018974 1102 60-61 0.42240251015553554 1102 62-63 0.556437570773916 1102 64-65 0.7137219331562861 1102 66-67 0.673750758137345 1102 68-69 0.7246653754761354 1102 70-71 0.6131058699056524 1102 72-73 0.5905207057158108 1102 74-75 0.566965746814347 1102 76-77 0.6005239828253757 1102 78-79 0.5443955643769556 1102 80-81 0.41596850684332054 1102 82-83 0.6591125749507185 1102 84-85 0.41052219273753465 1102 86-87 0.4011219820248044 1102 88-89 0.4581934477668774 1102 90-91 0.3125025799705412 1102 92-93 0.3061507227619664 1102 94-95 0.3736900080398051 1102 96-97 0.3201376100099651 1102 98-99 0.16795402444498464 1102 100-101 0.2778654043961666 1103 1 0.06721667273172827 1103 2 0.15476755742046677 1103 3 0.2574027450246845 1103 4 0.3028747926100479 1103 5 0.1983522804568949 1103 6 0.1807447791411505 1103 7 0.2153749684278239 1103 8 0.23572464330127474 1103 9 0.31063290654790165 1103 10-11 0.25278646888506273 1103 12-13 0.3164181282453953 1103 14-15 0.3579282804167221 1103 16-17 0.3767484422286671 1103 18-19 0.4525388433558106 1103 20-21 0.46064277552422794 1103 22-23 0.33957922813785046 1103 24-25 0.11767710900443973 1103 26-27 0.31153745181079984 1103 28-29 0.30716297527010283 1103 30-31 0.5096760492563419 1103 32-33 0.4556959005407819 1103 34-35 0.3710223152058063 1103 36-37 0.5545481151464031 1103 38-39 0.5861378334692731 1103 40-41 0.6070978638401741 1103 42-43 0.6117027615542696 1103 44-45 0.5255523762042316 1103 46-47 0.534158610179368 1103 48-49 0.8250037165905653 1103 50-51 0.9047693453390409 1103 52-53 0.8535334831487376 1103 54-55 0.9424974662607468 1103 56-57 0.8996629302426769 1103 58-59 0.7916662994241221 1103 60-61 0.8404163990444218 1103 62-63 0.9155174318850285 1103 64-65 0.8046160303785044 1103 66-67 0.8042316609151214 1103 68-69 0.8251462782539107 1103 70-71 0.7317898976834272 1103 72-73 0.7129582057158146 1103 74-75 0.8093008162587907 1103 76-77 0.7028295383809287 1103 78-79 0.6589285504880671 1103 80-81 0.8176143401766538 1103 82-83 0.860579588839606 1103 84-85 0.9708537899597545 1103 86-87 0.8713008014692463 1103 88-89 0.9451587255446547 1103 90-91 0.911572024414987 1103 92-93 0.9818764172064114 1103 94-95 0.9616014663731391 1103 96-97 0.864597679454409 1103 98-99 0.841601593889429 1103 100-101 1.0592126266183897 1104 1 0.255774365039418 1104 2 0.27173764289055313 1104 3 0.2903215484434938 1104 4 0.22288547637073464 1104 5 0.32113646849108335 1104 6 0.2817127278591016 1104 7 0.26977026757312217 1104 8 0.35229301936964674 1104 9 0.28249615441114884 1104 10-11 0.3097212979448969 1104 12-13 0.33772688892915426 1104 14-15 0.4166846906731294 1104 16-17 0.38772280120303293 1104 18-19 0.4039181168600834 1104 20-21 0.6363917071481637 1104 22-23 0.5455535871122095 1104 24-25 0.4853085192608475 1104 26-27 0.37649685352019446 1104 28-29 0.49042045390258693 1104 30-31 0.31390361335891015 1104 32-33 0.3965612851561673 1104 34-35 0.4130468878553799 1104 36-37 0.4395064484797473 1104 38-39 0.3623750129564556 1104 40-41 0.46672072708803825 1104 42-43 0.4661493427508461 1104 44-45 0.22543485483671333 1104 46-47 0.23615219992296232 1104 48-49 0.22281354565038924 1104 50-51 0.04904605474074941 1104 52-53 0.10263604725129483 1104 54-55 0.017168406431693484 1104 56-57 0.1501971182768571 1104 58-59 0.24288317976600382 1104 60-61 0.20234374947177258 1104 62-63 0.32873324385084146 1104 64-65 0.33073248336996386 1104 66-67 0.22752012245359055 1104 68-69 0.22787384235648034 1104 70-71 0.2566488720424047 1104 72-73 0.29670393221153546 1104 74-75 0.28161919232716315 1104 76-77 0.2694876580390506 1104 78-79 0.29198837954789525 1104 80-81 0.16544767350998768 1104 82-83 0.17129540080541972 1104 84-85 0.16862515748112017 1104 86-87 0.21635635702480727 1104 88-89 0.3480305204164509 1104 90-91 -0.03490660806364687 1104 92-93 0.007920220625216245 1104 94-95 0.15213885953552975 1104 96-97 0.11988720936894026 1104 98-99 0.07332595286378663 1104 100-101 0.12471369499445473 1105 1 0.33201407953886175 1105 2 0.3828662428157479 1105 3 0.31335016083595946 1105 4 0.39434606939737193 1105 5 0.42227592533353686 1105 6 0.3101321373259154 1105 7 0.12718336028807897 1105 8 0.29577938848405694 1105 9 0.3656071547020545 1105 10-11 0.36438704515014564 1105 12-13 0.4592616362590789 1105 14-15 0.550708399271393 1105 16-17 0.5929299657294749 1105 18-19 0.565255933217145 1105 20-21 0.48991488069261635 1105 22-23 0.5248693415900334 1105 24-25 0.30622276000390514 1105 26-27 0.14776939850627713 1105 28-29 0.11734521910226903 1105 30-31 0.1124918245129578 1105 32-33 0.27995305883359833 1105 34-35 0.13008588444765934 1105 36-37 0.07898679694002908 1105 38-39 0.18720644503058992 1105 40-41 0.14747891912561073 1105 42-43 0.2579076958240307 1105 44-45 0.2473910219812936 1105 46-47 0.20169291596003092 1105 48-49 0.15691439550285935 1105 50-51 0.0014043174261999525 1105 52-53 0.15651025246262407 1105 54-55 0.14028532867746435 1105 56-57 0.47384805540024644 1105 58-59 0.4621366757972538 1105 60-61 0.2816143100835049 1105 62-63 0.34528692585225684 1105 64-65 0.43136013266555295 1105 66-67 0.28352285486433715 1105 68-69 0.23989398219772795 1105 70-71 0.14418211810482262 1105 72-73 0.0702205864109331 1105 74-75 0.11811947284081725 1105 76-77 0.021416968598831687 1105 78-79 0.2695015740789195 1105 80-81 0.39416863515234013 1105 82-83 0.3150168299705314 1105 84-85 0.40772070874239574 1105 86-87 0.3485754277973605 1105 88-89 0.48389886600926957 1105 90-91 0.536100747627664 1105 92-93 0.4188016828285974 1105 94-95 0.39142570552314737 1105 96-97 0.46116674122282575 1105 98-99 0.2605394653102806 1105 100-101 0.30347374673363703 1106 1 0.10462233310908786 1106 2 0.15201703331144856 1106 3 0.3134619693433436 1106 4 0.2556866374737794 1106 5 0.2495199953415863 1106 6 0.2261158483235448 1106 7 0.19921511517834745 1106 8 0.24832894099519365 1106 9 0.3123582734242163 1106 10-11 0.2491035548389462 1106 12-13 0.3123549731091302 1106 14-15 0.3848087835613754 1106 16-17 0.36864624097081133 1106 18-19 0.2476046190371477 1106 20-21 0.3307177231133309 1106 22-23 0.34752655518187225 1106 24-25 0.44912836686607704 1106 26-27 0.3429609318946518 1106 28-29 0.42058645535396977 1106 30-31 0.28814617504251316 1106 32-33 0.14066052318229794 1106 34-35 0.30144946405276585 1106 36-37 0.29658192017785723 1106 38-39 0.34605607246298575 1106 40-41 0.3041935137353562 1106 42-43 0.03274495232994923 1106 44-45 0.23461029025035174 1106 46-47 0.2716837674120782 1106 48-49 0.18908259499727365 1106 50-51 0.27232254240403364 1106 52-53 0.37186681648206843 1106 54-55 0.22866151238234522 1106 56-57 0.419323569655667 1106 58-59 0.3870958067616499 1106 60-61 0.44074632566915994 1106 62-63 0.5775902830380701 1106 64-65 0.6108123092045048 1106 66-67 0.5065476986509694 1106 68-69 0.25193951724762087 1106 70-71 0.25868533793500603 1106 72-73 0.08355988286466243 1106 74-75 0.4612636045187415 1106 76-77 0.36395427632642097 1106 78-79 0.340332900592891 1106 80-81 0.4023580508684752 1106 82-83 0.5582420626341573 1106 84-85 0.5152416306306158 1106 86-87 0.6531488098549936 1106 88-89 0.7938033796327062 1106 90-91 0.7397169405575461 1106 92-93 0.5348342264307284 1106 94-95 0.5793249988678966 1106 96-97 0.7495180148841776 1106 98-99 0.7491786379145857 1106 100-101 0.7727225846896673 1107 1 0.2535934843259291 1107 2 0.2713279438938976 1107 3 0.32196313149811573 1107 4 0.3120535365714048 1107 5 0.2802460002636522 1107 6 0.28602014145999277 1107 7 0.153191393548596 1107 8 0.27087440175537836 1107 9 0.562072520074473 1107 10-11 0.39685410173530755 1107 12-13 0.4529881765545767 1107 14-15 0.3533485702717982 1107 16-17 0.43686438425765317 1107 18-19 0.5059784568823744 1107 20-21 0.610072727215055 1107 22-23 0.5382458948045183 1107 24-25 0.47509739885951063 1107 26-27 0.39880315229388685 1107 28-29 0.3781678061879816 1107 30-31 0.41095141157518356 1107 32-33 0.41975387155527955 1107 34-35 0.2789450205198136 1107 36-37 0.4890988397840914 1107 38-39 0.37892044216492593 1107 40-41 0.4059577672218211 1107 42-43 0.5554902011677854 1107 44-45 0.7012528592960194 1107 46-47 0.6584194797445875 1107 48-49 0.5627814943683447 1107 50-51 0.4951364950974977 1107 52-53 0.6559972512646794 1107 54-55 0.7233863551496427 1107 56-57 0.8567160703392886 1107 58-59 0.7040237873468271 1107 60-61 0.7220009401072289 1107 62-63 0.7323773352666691 1107 64-65 0.6794469482529024 1107 66-67 0.6603476029441069 1107 68-69 0.6932912057901461 1107 70-71 0.6370362744950242 1107 72-73 0.6660113458124286 1107 74-75 0.7235520239882547 1107 76-77 0.7170517606031517 1107 78-79 0.7688222702948337 1107 80-81 0.6894694126404204 1107 82-83 0.7313525356995143 1107 84-85 0.6356750459984006 1107 86-87 0.6126244729668322 1107 88-89 0.669100754530163 1107 90-91 0.6578135703087078 1107 92-93 0.5402049196218712 1107 94-95 0.7449154760349757 1107 96-97 0.6344141045751819 1107 98-99 0.19878999968653233 1107 100-101 0.04963774739133342 1108 1 -0.08417405574509118 1108 2 0.08136171087554445 1108 3 0.04064630646692535 1108 4 0.23236974210499994 1108 5 0.27380776242893035 1108 6 0.2354844941722618 1108 7 0.1437163959480614 1108 8 0.18191315089375593 1108 9 0.23516136999027282 1108 10-11 0.07066017281174908 1108 12-13 0.10261988087098217 1108 14-15 0.24393831453271986 1108 16-17 0.24225436235710873 1108 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2629.0 9 3291.0 10 4090.0 11 5179.0 12 6821.0 13 8444.0 14 9479.0 15 11704.0 16 15070.0 17 20113.0 18 17934.0 19 11252.0 20 10672.0 21 11157.0 22 12043.0 23 13646.0 24 15352.0 25 17665.0 26 20288.0 27 23963.0 28 29142.0 29 34646.0 30 43041.0 31 53333.0 32 68075.0 33 89116.0 34 121814.0 35 176001.0 36 266568.0 37 403978.0 38 384522.0 39 70566.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.094143463775005 19.653305572103978 14.819511891795392 22.433039072325624 2 14.814926238245803 20.75081453313638 41.300515344510664 23.13374388410715 3 18.406805955361364 26.095646196219256 30.411693711952292 25.085854136467084 4 12.08094952263611 17.28744782945981 38.967275287448835 31.664327360455246 5 12.933768818156327 37.963879373178756 34.84447101987623 14.25788078878869 6 29.781052137417447 37.652070811231575 18.342939741439494 14.223937309911486 7 26.923530134407052 33.5812045740016 22.027713776409446 17.467551515181903 8 25.373961580093635 35.1265565639775 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32-33 20.788198480465873 27.376004857054546 30.568736120507893 21.267060541971684 34-35 21.24394530412791 27.331391619345847 30.351350965179247 21.073312111346993 36-37 21.284763948820608 27.030814220775675 30.351854553549 21.332567276854718 38-39 20.873922574515795 27.529250449018676 30.434955900287147 21.161871076178386 40-41 21.04683575399949 27.427066674019745 30.444393195730534 21.081704376250233 42-43 20.973769815396796 27.401479823696583 30.0721512441283 21.552599116778328 44-45 21.07372903619121 27.471638821875562 29.951198920299927 21.503433221633305 46-47 21.322991570872006 27.689532550130824 29.523931995441764 21.463543883555406 48-49 21.263851917992586 27.871110577454555 29.309601882459578 21.55543562209328 50-51 21.18006465365973 27.98451641108016 29.22156412122962 21.613854814030486 52-53 21.45088505713127 28.10459351344143 28.89494574647664 21.54957568295066 54-55 21.37294071551887 27.952473547881535 28.9747114012397 21.699874335359894 56-57 21.405858596442588 28.294548673196385 28.70184337954337 21.59774935081766 58-59 21.509115461679013 27.932089829292057 28.941813305939444 21.616981403089483 60-61 21.49738714433903 27.96307057041625 29.219913554912587 21.319628730332138 62-63 21.258214098242796 28.0752576094624 29.300071343018537 21.366456949276266 64-65 21.25653891160023 28.03018452841124 29.104335811025578 21.608940748962947 66-67 21.233093413356798 28.227650714620577 28.700872185578223 21.838383686444395 68-69 21.18309285701213 28.10402737145437 29.047848148297646 21.66503162323585 70-71 21.374524503654655 28.00415525696924 28.996783261425392 21.624536977950708 72-73 21.303789489835676 27.94335697262018 28.972225049754822 21.780628487789322 74-75 21.16757832273502 28.440334770036774 28.68548780766042 21.706599099567786 76-77 21.23294369463346 28.157246998972912 28.808806891128512 21.801002415265117 78-79 21.23849362733619 28.07429567735973 28.73929218476789 21.947918510536194 80-81 21.20942559770196 28.373422561134493 28.5448878052524 21.872264035911147 82-83 21.341075337845464 28.19131996078009 28.733352224426184 21.734252476948267 84-85 21.176744317821406 28.204260320788954 28.582675250389933 22.036320110999707 86-87 21.092364649036796 28.346114784105087 28.64302685345661 21.918493713401507 88-89 21.261546754247966 28.10394429227751 28.59473384018502 22.0397751132895 90-91 21.17667796059403 27.981542916129992 28.776748066020456 22.065031057255524 92-93 21.26284210185817 27.967954519068282 28.752090356039446 22.0171130230341 94-95 21.077785193070316 27.765613021008566 28.841161926757074 22.31543985916404 96-97 21.030669616833503 27.865462363434645 28.909696061777996 22.194171957953852 98-99 20.871028285789155 27.969844873866766 28.73099476059072 22.428132079753365 100-101 20.89832058603069 27.93609053573278 28.78767170866184 22.377917169574694 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 380.0 1 296.0 2 271.5 3 339.0 4 316.0 5 347.0 6 598.0 7 1233.0 8 2144.5 9 2679.0 10 2468.0 11 2256.0 12 2354.5 13 2459.0 14 2700.0 15 2933.5 16 3045.0 17 3205.0 18 3400.0 19 3624.0 20 4080.0 21 4815.0 22 5808.0 23 7129.5 24 8621.5 25 10770.5 26 13929.5 27 17263.5 28 19916.5 29 23232.5 30 28065.0 31 32855.0 32 38176.0 33 43982.0 34 50596.0 35 56669.5 36 64147.0 37 70700.5 38 71328.5 39 70653.5 40 69574.0 41 69076.0 42 68769.5 43 70197.5 44 72991.0 45 74088.5 46 72883.0 47 72362.5 48 76103.0 49 74682.5 50 67856.5 51 62575.5 52 58444.0 53 55988.5 54 52907.0 55 47979.5 56 42850.0 57 37523.5 58 33322.5 59 29612.5 60 25151.5 61 20383.0 62 15870.0 63 11991.5 64 8761.5 65 6526.5 66 4778.5 67 3598.0 68 2666.5 69 1723.0 70 1140.5 71 790.5 72 529.5 73 399.5 74 313.5 75 225.5 76 147.5 77 75.5 78 44.5 79 26.5 80 19.0 81 15.0 82 12.5 83 9.0 84 5.0 85 8.5 86 8.5 87 6.0 88 6.0 89 3.0 90 0.5 91 1.0 92 1.5 93 2.0 94 1.5 95 0.5 96 0.0 97 0.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024215005181409493 2 0.011881896952368633 3 0.013034992437197657 4 0.02256056383361133 5 0.0573539667236697 6 0.04126076452236027 7 0.015842529269824844 8 0.04898149081208504 9 0.029629540501476214 10-11 0.023237380748619668 12-13 0.035069143114691385 14-15 0.01849966234356129 16-17 0.018449527757264376 18-19 0.01749697061762301 20-21 0.014238222508323593 22-23 0.007570322530834024 24-25 0.0021056526244703907 26-27 1.0026917259382814E-4 28-29 0.002331258262806504 30-31 0.0 32-33 3.2587481092994145E-4 34-35 7.52018794453711E-5 36-37 0.0 38-39 1.7547105203919924E-4 40-41 5.013458629691407E-5 42-43 7.52018794453711E-5 44-45 1.504037588907422E-4 46-47 0.0 48-49 3.2587481092994145E-4 50-51 1.504037588907422E-4 52-53 7.01884208156797E-4 54-55 0.0018299123998373635 56-57 3.2587481092994145E-4 58-59 0.0012784319505713087 60-61 0.0035094210407839847 62-63 0.0021056526244703907 64-65 0.001654441347798164 66-67 0.010829070640133439 68-69 0.014564097319253537 70-71 0.02659639803051291 72-73 0.01764737437651375 74-75 0.018374325877819005 76-77 0.0324370773341034 78-79 0.020555180381734768 80-81 0.04675050172187237 82-83 0.037400401377497894 84-85 0.019627690535241858 86-87 0.01351127100701834 88-89 0.014714501078144277 90-91 0.024641149164933263 92-93 0.03271281755873643 94-95 0.012734184919416174 96-97 0.019727959707835686 98-99 0.03050689576167221 100-101 0.04063408219364885 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1994631.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.92025365849943 #Duplication Level Percentage of deduplicated Percentage of total 1 78.01972693065112 43.6288292032887 2 12.821809225350634 14.339976484849911 3 3.955087921159069 6.635085593786469 4 1.7189882306489972 3.845050315754682 5 0.8910381187168439 2.4913538809019022 6 0.5368245874659904 1.8011620260730503 7 0.35274040317269423 1.3807732974712894 8 0.24862397670049702 1.1122492674141318 9 0.18879806612568242 0.9501872173184082 >10 1.0983378584843786 11.618885388103715 >50 0.10689418075808643 4.1239539827474925 >100 0.05852426549428855 5.90335968414243 >500 0.001887273817439889 0.6840294291518038 >1k 5.39221090697111E-4 0.6412618849443106 >5k 1.7974036356570365E-4 0.8438423440517179 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9662 0.4844003728007837 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7129 0.35740946571070037 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4561 0.22866384810022508 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2455 0.12308040935892403 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.0134586296914065E-5 0.0 4 0.0 0.0 0.0 5.0134586296914065E-5 0.0 5 0.0 0.0 0.0 5.0134586296914065E-5 0.0 6 0.0 0.0 0.0 5.0134586296914065E-5 0.0 7 0.0 0.0 0.0 5.0134586296914065E-5 0.0 8 0.0 0.0 0.0 5.0134586296914065E-5 0.0 9 0.0 0.0 0.0 1.0026917259382813E-4 0.0 10-11 0.0 0.0 0.0 1.0026917259382813E-4 0.0 12-13 0.0 0.0 0.0 1.2533646574228516E-4 0.0 14-15 0.0 0.0 0.0 1.7547105203919924E-4 0.0 16-17 0.0 0.0 0.0 2.2560563833611328E-4 0.0 18-19 0.0 0.0 0.0 2.506729314845703E-4 0.0 20-21 0.0 0.0 0.0 3.2587481092994145E-4 0.0 22-23 0.0 0.0 0.0 6.266823287114259E-4 0.0 24-25 0.0 0.0 0.0 9.525571396413673E-4 0.0 26-27 0.0 0.0 0.0 0.0013034992437197658 0.0 28-29 0.0 0.0 0.0 0.0024816620216972464 0.0 30-31 0.0 0.0 0.0 0.005088660509136778 0.0 32-33 0.0 0.0 0.0 0.008247139445842364 0.0 34-35 0.0 0.0 0.0 0.012533646574228516 0.0 36-37 0.0 0.0 0.0 0.01611826949445787 0.0 38-39 0.0 0.0 0.0 0.020479978502289397 0.0 40-41 0.0 0.0 0.0 0.027574022463302737 0.0 42-43 0.0 0.0 0.0 0.035971565668035846 0.0 44-45 0.0 0.0 0.0 0.04394296488924518 0.0 46-47 0.0 0.0 0.0 0.0532679979404712 0.0 48-49 0.0 0.0 0.0 0.0627183674574395 0.0 50-51 0.0 0.0 0.0 0.07447492794406585 0.0 52-53 0.0 0.0 0.0 0.10177321018273555 0.0 54-55 0.0 0.0 0.0 0.13927388073282726 0.0 56-57 0.0 0.0 0.0 0.1743179565543702 5.0134586296914065E-5 58-59 0.0 0.0 0.0 0.23252421124508743 5.0134586296914065E-5 60-61 0.0 0.0 0.0 0.3019606132663134 5.0134586296914065E-5 62-63 0.0 0.0 0.0 0.3483100382978105 5.0134586296914065E-5 64-65 0.0 0.0 0.0 0.38520909381233925 5.0134586296914065E-5 66-67 0.0 0.0 0.0 0.4841747671624476 5.0134586296914065E-5 68-69 0.0 0.0 0.0 0.5917134547693282 5.0134586296914065E-5 70-71 0.0 0.0 0.0 0.6406949455814133 5.0134586296914065E-5 72-73 0.0 0.0 0.0 0.7369282839783399 5.0134586296914065E-5 74-75 0.0 0.0 0.0 0.881792171083273 5.0134586296914065E-5 76-77 5.0134586296914065E-5 0.0 0.0 1.019135870243669 5.0134586296914065E-5 78-79 5.0134586296914065E-5 0.0 0.0 1.1522682641551245 5.0134586296914065E-5 80-81 5.0134586296914065E-5 0.0 0.0 1.3143032470667508 5.0134586296914065E-5 82-83 5.0134586296914065E-5 0.0 0.0 1.461698930779678 5.0134586296914065E-5 84-85 5.0134586296914065E-5 0.0 0.0 1.6261153065404077 5.0134586296914065E-5 86-87 5.0134586296914065E-5 0.0 0.0 1.9043371931951323 5.0134586296914065E-5 88-89 5.0134586296914065E-5 0.0 0.0 2.2741299017211705 5.0134586296914065E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12100 0.0 30.08532 1 GGTATCA 8380 0.0 29.433004 1 GTACATG 8855 0.0 25.492727 1 ATCAACG 14390 0.0 25.167385 3 TCAACGC 14450 0.0 24.997103 4 CAACGCA 14675 0.0 24.646229 5 TATCAAC 14670 0.0 24.622232 2 AACGCAG 14965 0.0 24.167408 6 TACATGG 9170 0.0 23.996923 2 ACATGGG 8945 0.0 23.484745 3 GAGTACT 7765 0.0 22.457405 12-13 ACGCAGA 16575 0.0 21.588919 7 CGCAGAG 16630 0.0 21.346066 8 GTACTTT 8405 0.0 20.631731 14-15 GCAGAGT 17290 0.0 20.281834 9 CATGGGA 5630 0.0 20.175924 4 AGAGTAC 12970 0.0 20.167511 10-11 AGTACTT 8320 0.0 20.102697 12-13 GTATAGG 835 0.0 19.351011 1 CATGGGG 3485 0.0 18.820026 4 >>END_MODULE