FastQCFastQC Report
Thu 26 May 2016
SRR937049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994631
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114960.576347204069324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93670.469610669843194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62540.3135417027009006No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32350.162185386670517No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25390.12729171460786481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88000.034.066431
GTATCAA132300.030.9188441
ATCAACG163650.024.9302373
TCAACGC165000.024.6974774
TATCAAC166600.024.4342292
CAACGCA167000.024.405375
AACGCAG170350.023.9533146
GTACATG86600.021.8345951
GAGTACT106950.021.6487212-13
ACGCAGA190450.021.3005837
AGAGTAC155650.020.9663210-11
CGCAGAG191050.020.9601868
AGTACTT112500.020.55960712-13
GTACTTT113450.020.15820914-15
ACATGGG88050.020.0660933
TACATGG91100.020.0219172
GCAGAGT200750.019.5688159
GTATAGT6500.018.2728611
GTATAGG9500.018.0035781
TACTTTT126950.017.99585714-15
Produced by FastQC (version 0.11.5)