##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937049_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1994631 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.201683419138675 33.0 31.0 34.0 31.0 34.0 2 32.32106389602889 33.0 31.0 34.0 31.0 34.0 3 32.42661374459737 34.0 31.0 34.0 31.0 34.0 4 36.07772415048197 37.0 35.0 37.0 35.0 37.0 5 35.99271343922761 37.0 35.0 37.0 35.0 37.0 6 36.04144927056684 37.0 35.0 37.0 35.0 37.0 7 35.9889037120149 37.0 35.0 37.0 35.0 37.0 8 35.948472173549895 37.0 35.0 37.0 35.0 37.0 9 37.63691680315808 39.0 37.0 39.0 35.0 39.0 10-11 37.543984325922935 39.0 37.0 39.0 35.0 39.0 12-13 37.455034038877365 39.0 37.0 39.0 35.0 39.0 14-15 38.6467975780984 40.0 38.0 41.0 34.0 41.0 16-17 38.56550158901571 40.0 38.0 41.0 34.0 41.0 18-19 38.745865275331624 40.0 38.0 41.0 34.5 41.0 20-21 38.78448670455839 40.0 38.0 41.0 35.0 41.0 22-23 38.701402916128345 40.0 38.0 41.0 34.0 41.0 24-25 38.59925921135287 40.0 38.0 41.0 34.0 41.0 26-27 38.41944650414037 40.0 38.0 41.0 34.0 41.0 28-29 38.3388975203935 40.0 38.0 41.0 34.0 41.0 30-31 38.30553144917531 40.0 38.0 41.0 34.0 41.0 32-33 38.12176061637466 40.0 37.0 41.0 34.0 41.0 34-35 37.965887675464785 40.0 37.0 41.0 33.0 41.0 36-37 37.792856924413584 40.0 37.0 41.0 33.0 41.0 38-39 37.63018072014322 40.0 37.0 41.0 33.0 41.0 40-41 37.44668913698824 40.0 37.0 41.0 33.0 41.0 42-43 37.17860045291585 40.0 36.0 41.0 32.0 41.0 44-45 36.87309933516525 40.0 36.0 41.0 31.0 41.0 46-47 36.46116023464992 39.0 36.0 41.0 30.5 41.0 48-49 36.05317850770393 39.0 35.5 41.0 29.5 41.0 50-51 35.74782904707688 39.0 35.0 40.0 28.5 41.0 52-53 35.51133868870984 39.0 35.0 40.0 28.0 41.0 54-55 35.235590191870074 38.5 35.0 40.0 27.0 41.0 56-57 34.95688199972827 38.0 34.5 40.0 26.0 41.0 58-59 34.61200442588128 38.0 34.0 40.0 26.0 41.0 60-61 34.29205226430352 38.0 34.0 40.0 25.0 41.0 62-63 33.939368986043036 37.0 33.5 40.0 24.5 41.0 64-65 33.56358494378158 37.0 33.0 39.0 23.0 40.5 66-67 33.17202354721249 36.0 32.5 39.0 22.0 40.0 68-69 32.859951539908884 36.0 32.0 39.0 22.0 40.0 70-71 32.387433816079266 35.0 31.5 38.5 20.5 40.0 72-73 31.87877457033406 35.0 31.0 37.5 19.0 39.5 74-75 31.35083657077424 35.0 31.0 37.0 18.0 39.0 76-77 29.96155053240424 33.5 29.5 35.5 16.0 38.0 78-79 30.691799886796105 34.0 30.5 36.0 16.0 38.5 80-81 30.4366346457064 34.0 30.0 36.0 10.5 37.5 82-83 30.090546321600335 34.0 30.0 35.0 7.5 37.0 84-85 29.71278622461999 34.0 30.0 35.0 7.0 36.5 86-87 29.36794725440445 34.0 29.0 35.0 2.0 36.0 88-89 28.953074528571953 34.0 29.0 35.0 2.0 36.0 90-91 28.47788914340547 34.0 29.0 35.0 2.0 35.0 92-93 27.98758241499305 33.0 28.0 35.0 2.0 35.0 94-95 27.385120606267527 33.0 26.5 35.0 2.0 35.0 96-97 26.695812408410376 33.0 25.5 34.0 2.0 35.0 98-99 25.846102612463156 32.0 24.5 34.0 2.0 35.0 100-101 22.24389674080068 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17132726606091353 1101 2 0.13400827819361183 1101 3 0.11114454228084725 1101 4 0.17971853176499053 1101 5 0.18861535057152423 1101 6 0.1699881908057037 1101 7 0.16796593568889762 1101 8 0.17152530806104238 1101 9 0.2687644510362617 1101 10-11 0.21308930967214934 1101 12-13 0.22862244798949405 1101 14-15 0.18476871599343525 1101 16-17 0.30534353857065355 1101 18-19 0.2872581671507888 1101 20-21 0.28851372084579197 1101 22-23 0.29433246393656987 1101 24-25 0.31377960799186866 1101 26-27 0.172376833104245 1101 28-29 0.27389828599562094 1101 30-31 0.34280445848990126 1101 32-33 0.3062011941108338 1101 34-35 0.31540711083317063 1101 36-37 0.33381644378131625 1101 38-39 0.3505327627727084 1101 40-41 0.45402884665501375 1101 42-43 0.4175148127681254 1101 44-45 0.2830094705132282 1101 46-47 0.2553328935509569 1101 48-49 0.4266685913042636 1101 50-51 0.3419355083323694 1101 52-53 0.5126467681323916 1101 54-55 0.5478361002064531 1101 56-57 0.5632031696311728 1101 58-59 0.48482062817303273 1101 60-61 0.6830673631165141 1101 62-63 0.6274889946674733 1101 64-65 0.4733482212981741 1101 66-67 0.2946815997617733 1101 68-69 0.21262516117422336 1101 70-71 0.35179965563484217 1101 72-73 0.39484168383390283 1101 74-75 0.45893394458184034 1101 76-77 0.24122298395345965 1101 78-79 0.5276983672160291 1101 80-81 0.7419973582762758 1101 82-83 0.7059775565933393 1101 84-85 0.3961225879972403 1101 86-87 0.16418161143834453 1101 88-89 0.3851805995279243 1101 90-91 0.625968371745369 1101 92-93 0.5449587537369496 1101 94-95 0.3819790394887086 1101 96-97 0.47410420649669405 1101 98-99 0.6271645887843924 1101 100-101 1.168917030973244 1102 1 0.21518547476476613 1102 2 0.1944998909664477 1102 3 0.28070414268056254 1102 4 0.20397110575672173 1102 5 0.16656519562676664 1102 6 0.11750346072396667 1102 7 0.1246845593752326 1102 8 0.1793792108725043 1102 9 0.20557077850864403 1102 10-11 0.2222917862620264 1102 12-13 0.19879786257900633 1102 14-15 0.3372791298531368 1102 16-17 0.3054312563915502 1102 18-19 0.17676862451852315 1102 20-21 0.3157557181735555 1102 22-23 0.1695443901918452 1102 24-25 0.2801496480754082 1102 26-27 0.20917445896879627 1102 28-29 0.3004731015776727 1102 30-31 0.34475775892553884 1102 32-33 0.31339300281020144 1102 34-35 0.29282977178484515 1102 36-37 0.30536587031728857 1102 38-39 0.4308507082950257 1102 40-41 0.37463176281642774 1102 42-43 0.33471427747889493 1102 44-45 0.2803128492633391 1102 46-47 0.20636161306726564 1102 48-49 0.29670990288919086 1102 50-51 0.46292750566013297 1102 52-53 0.4787926014657202 1102 54-55 0.4476372614500477 1102 56-57 0.4344907760153447 1102 58-59 0.5458539862077032 1102 60-61 0.46365895315289407 1102 62-63 0.41316277981074734 1102 64-65 0.3613494058396256 1102 66-67 0.3285798007745271 1102 68-69 0.2998638673293499 1102 70-71 0.29394448968675846 1102 72-73 0.3658979607808135 1102 74-75 0.49194233601945925 1102 76-77 0.29008847674516147 1102 78-79 0.407122678666223 1102 80-81 0.6169468328114398 1102 82-83 0.4796959290558398 1102 84-85 0.46582544914203794 1102 86-87 0.36014550397629463 1102 88-89 0.5206795455105855 1102 90-91 0.6050022336312004 1102 92-93 0.541004960678027 1102 94-95 0.5413554780609715 1102 96-97 0.42261788065235706 1102 98-99 0.5365383958220278 1102 100-101 0.7976480207671024 1103 1 0.1947757525425473 1103 2 0.19754155763311587 1103 3 0.16393678156944702 1103 4 0.1332731890900547 1103 5 0.12377352896010052 1103 6 0.115114571835079 1103 7 0.06571928159745255 1103 8 0.10699032198361635 1103 9 0.17251869517530594 1103 10-11 0.20801574459535743 1103 12-13 0.2602058486901129 1103 14-15 0.26498051874202844 1103 16-17 0.2909520897248754 1103 18-19 0.2554092495185216 1103 20-21 0.12814113484022016 1103 22-23 0.26300272352517595 1103 24-25 0.21559062029762543 1103 26-27 0.28471959785768064 1103 28-29 0.2909470599110051 1103 30-31 0.24101123114775902 1103 32-33 0.2633061972546429 1103 34-35 0.3132134523403991 1103 36-37 0.2099544119839578 1103 38-39 0.16519098607280114 1103 40-41 0.3607203044830882 1103 42-43 0.4525944858122273 1103 44-45 0.38315139093000283 1103 46-47 0.20416196028948264 1103 48-49 0.2553505278891919 1103 50-51 0.3755629223267931 1103 52-53 0.4382023236879462 1103 54-55 0.4359688586722754 1103 56-57 0.24190917879311513 1103 58-59 0.3442012084299293 1103 60-61 0.22660513370845337 1103 62-63 0.4519127798107405 1103 64-65 0.3979674613951829 1103 66-67 0.4210259813300823 1103 68-69 0.2078135201071305 1103 70-71 0.11964587857564624 1103 72-73 0.26640837744749035 1103 74-75 0.4031940721305709 1103 76-77 0.31948778230071184 1103 78-79 0.3987424703328877 1103 80-81 0.4702246105892165 1103 82-83 0.47190947072250466 1103 84-85 0.49469871303092816 1103 86-87 0.2768173789762969 1103 88-89 0.3003791982883648 1103 90-91 0.4158859141867559 1103 92-93 0.2104789190113614 1103 94-95 -0.023347646939029687 1103 96-97 0.001973783430134546 1103 98-99 0.17740297915536019 1103 100-101 0.14524003465599122 1104 1 0.12363900040579523 1104 2 0.19137489096645055 1104 3 0.18828293541560015 1104 4 0.13614925746612272 1104 5 0.09885686229343094 1104 6 -0.0013533768828679626 1104 7 0.05605475168292173 1104 8 0.1286847664280586 1104 9 0.19128792594453614 1104 10-11 0.160583052287663 1104 12-13 0.1641257204849822 1104 14-15 0.08037474951125034 1104 16-17 0.2052576452804331 1104 18-19 0.2597447196039937 1104 20-21 0.19833451090859455 1104 22-23 0.16019609959355563 1104 24-25 0.14675621858823007 1104 26-27 0.038508059396143324 1104 28-29 0.049661803500747226 1104 30-31 0.18004755593408817 1104 32-33 0.244930128878579 1104 34-35 0.07720811046005593 1104 36-37 0.05485505300959659 1104 38-39 0.20776043051724713 1104 40-41 0.16682927884206578 1104 42-43 0.072878673846418 1104 44-45 -0.0020355748819582686 1104 46-47 0.09958076370828195 1104 48-49 0.13922125438492117 1104 50-51 0.28589091377978804 1104 52-53 0.2638486912093114 1104 54-55 0.29179791850133796 1104 56-57 0.21898930699824604 1104 58-59 0.20968945629316948 1104 60-61 0.2693380396913554 1104 62-63 0.3549629934859553 1104 64-65 0.2653424613951856 1104 66-67 0.26433367363777194 1104 68-69 0.24732313549174734 1104 70-71 0.17216297259273716 1104 72-73 0.1668795312936382 1104 74-75 0.16116308922458344 1104 76-77 0.0884258164887477 1104 78-79 0.10079588913631099 1104 80-81 0.49867760204221057 1104 82-83 0.46366694935498387 1104 84-85 0.3912628155950273 1104 86-87 0.2591004986344174 1104 88-89 0.26877983931400706 1104 90-91 0.3925589911098335 1104 92-93 0.35039665405409437 1104 94-95 0.20636282314644205 1104 96-97 0.12654750137885173 1104 98-99 -0.017434627682245463 1104 100-101 0.36474537653633377 1105 1 0.21854092452165474 1105 2 0.22938812705504574 1105 3 0.17809561463266022 1105 4 0.10901891212265014 1105 5 0.13044451761487608 1105 6 0.15551219474161826 1105 7 0.15390440675502504 1105 8 0.12631104951828576 1105 9 0.21195035373824567 1105 10-11 0.2185765225532137 1105 12-13 0.23257429817687836 1105 14-15 0.30360504602457183 1105 16-17 0.24228830438362792 1105 18-19 0.24389907483816842 1105 20-21 0.2020467714330536 1105 22-23 0.2051698403989448 1105 24-25 0.1394170204416909 1105 26-27 0.22442030018074632 1105 28-29 0.15502701669112895 1105 30-31 0.15189255655744915 1105 32-33 0.2230666870799709 1105 34-35 0.32810666321740456 1105 36-37 0.21691389334537803 1105 38-39 0.2237737341369126 1105 40-41 0.18289102301361737 1105 42-43 0.3094516801216116 1105 44-45 0.2250242524463104 1105 46-47 0.17504724752161138 1105 48-49 0.11951296260916422 1105 50-51 0.0917732590817053 1105 52-53 0.11340311605243159 1105 54-55 0.18132956459700011 1105 56-57 0.14242349537875043 1105 58-59 0.08706200439606704 1105 60-61 0.10425757383089973 1105 62-63 0.050695419825153465 1105 64-65 -0.1288515553525187 1105 66-67 -0.17512420775689463 1105 68-69 0.12950233696287938 1105 70-71 -0.29235880357814636 1105 72-73 -0.019676981817777772 1105 74-75 -0.028503748867091616 1105 76-77 -0.21596188454603293 1105 78-79 -0.019859006346383268 1105 80-81 -0.13058828334198935 1105 82-83 0.03387237365785367 1105 84-85 0.011632262463663778 1105 86-87 -0.20048930209699733 1105 88-89 -0.08578071527186992 1105 90-91 -0.12317081190724721 1105 92-93 -0.05070890738878475 1105 94-95 -0.1962754337209489 1105 96-97 0.04387869969161784 1105 98-99 0.15826665644691573 1105 100-101 0.46824309606423853 1106 1 0.1435954590414994 1106 2 0.10694092870229355 1106 3 0.14387074383360243 1106 4 0.06369824150095837 1106 5 0.08824365474626461 1106 6 0.1075453894451428 1106 7 0.1324970593752326 1106 8 0.10521778529598436 1106 9 0.17891492159040467 1106 10-11 0.07865725402931645 1106 12-13 0.18138352164609017 1106 14-15 0.27643334893070204 1106 16-17 0.2118614188653467 1106 18-19 -0.09877759534521147 1106 20-21 0.18039637593036417 1106 22-23 -0.011043922177123022 1106 24-25 0.04963176285527737 1106 26-27 0.0786795035180603 1106 28-29 0.0643660850682366 1106 30-31 0.0719016923636957 1106 32-33 -0.03698206270342297 1106 34-35 -0.0726830361291988 1106 36-37 0.04397878305313441 1106 38-39 0.11489486447951691 1106 40-41 0.2576039522818334 1106 42-43 0.15780701201767755 1106 44-45 -0.16084363003435698 1106 46-47 -0.005816551240918955 1106 48-49 0.05998050692483048 1106 50-51 0.13358231436033918 1106 52-53 0.15946280586823747 1106 54-55 -0.06203009310969776 1106 56-57 0.029915468101300746 1106 58-59 0.009068084740199822 1106 60-61 0.05538212532270137 1106 62-63 0.23585014878349142 1106 64-65 -0.10564810464255459 1106 66-67 -0.016771188481236265 1106 68-69 0.008766350295807968 1106 70-71 -0.17567356586879868 1106 72-73 -0.2057166225525151 1106 74-75 0.05723075976159819 1106 76-77 0.03981823303446674 1106 78-79 -0.015092173272979181 1106 80-81 0.1561567384718181 1106 82-83 0.15987068665542026 1106 84-85 0.17061695202463767 1106 86-87 -0.03475835477632572 1106 88-89 -0.07888888347264 1106 90-91 -0.14114474619060147 1106 92-93 0.022697996579491786 1106 94-95 0.02354805536705129 1106 96-97 0.06663730544271118 1106 98-99 -0.07426971476497712 1106 100-101 0.08940067406898677 1107 1 0.27125884432998504 1107 2 0.1791647460389143 1107 3 0.22527011490278426 1107 4 0.14604613594995897 1107 5 0.1488749782354617 1107 6 0.10386819502348743 1107 7 0.15532314633175304 1107 8 0.2567101287469029 1107 9 0.16979405749415122 1107 10-11 0.19717516488520914 1107 12-13 0.24985802260315637 1107 14-15 0.3763718230898476 1107 16-17 0.2478119931065237 1107 18-19 0.22669427367311812 1107 20-21 0.1595517628595431 1107 22-23 0.24786262690682292 1107 24-25 0.17284665894496953 1107 26-27 0.19100945293014604 1107 28-29 0.23686010338926877 1107 30-31 0.21413200409462263 1107 32-33 0.28122890256864963 1107 34-35 0.147986399200299 1107 36-37 0.22847615111439268 1107 38-39 0.3155774595027552 1107 40-41 0.24480726100482997 1107 42-43 0.2034737128653603 1107 44-45 0.11976974841793009 1107 46-47 -0.009103740193609156 1107 48-49 -0.07408908563737526 1107 50-51 -0.1536931163205466 1107 52-53 -0.14561410143282671 1107 54-55 -0.09547075485429701 1107 56-57 -0.016235748743106626 1107 58-59 -0.116504105579736 1107 60-61 -0.09399390010797504 1107 62-63 -0.0972659641506084 1107 64-65 -0.23794558208307848 1107 66-67 -0.19848126504673047 1107 68-69 -0.059519813226209806 1107 70-71 -0.19966813108619164 1107 72-73 -0.24251915878440045 1107 74-75 0.012169917541196895 1107 76-77 -0.04006777325484023 1107 78-79 0.04402749448748011 1107 80-81 0.23009900672448325 1107 82-83 0.08369691033603388 1107 84-85 4.378434657112962E-4 1107 86-87 0.005822209894169816 1107 88-89 0.00881398089706309 1107 90-91 0.19782794317226404 1107 92-93 0.3018605615234371 1107 94-95 0.2740823047517935 1107 96-97 0.25377088487940824 1107 98-99 0.1063111917157471 1107 100-101 0.28458302982507533 1108 1 0.03751734060863754 1108 2 0.09729752793212754 1108 3 0.1077585756693864 1108 4 0.07559896338933925 1108 5 -0.0027197481821872316 1108 6 0.013169558790728786 1108 7 0.09539591548600868 1108 8 0.08415963096749834 1108 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4634.0 13 6809.0 14 9062.0 15 12575.0 16 17006.0 17 23840.0 18 21407.0 19 12502.0 20 10302.0 21 10367.0 22 11086.0 23 12118.0 24 13896.0 25 16578.0 26 20084.0 27 24901.0 28 30762.0 29 38311.0 30 47736.0 31 60993.0 32 76768.0 33 100686.0 34 136835.0 35 195038.0 36 299692.0 37 446259.0 38 308612.0 39 19070.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.58182543973056 20.281816713823343 15.391798249001285 21.744559597444812 2 15.214301292801974 21.524488532242337 40.51010150388192 22.751108671073776 3 18.947063391674952 26.236632239246255 30.61408350717501 24.202220861903783 4 12.688556840788037 18.027056728486173 38.24023976448694 31.04414666623885 5 12.98065305344851 38.26524217524052 34.597795079789236 14.156309691521734 6 30.042599357976492 38.06709110607426 17.67890903129451 14.211400504654744 7 27.182320940564946 33.81688141816707 21.597879507537986 17.402918133729997 8 25.752031328100284 35.34804181826112 20.144026639513772 18.755900214124818 9 26.32511978406031 17.157208526288823 21.249444132774435 35.26822755687643 10-11 24.13803131733053 27.780329419126648 27.74367603826593 20.33796322527689 12-13 25.501684271426644 25.248529677920377 28.46037688173903 20.78940916891395 14-15 22.449741330602002 26.3593617065011 27.28735289885698 23.903544064039913 16-17 20.913196704853355 29.763006230978622 27.8575154646437 21.466281599524322 18-19 20.752622673434857 27.884890643604688 30.972664034592967 20.38982264836749 20-21 21.73206902887751 26.846215145833945 31.26927534117495 20.152440484113594 22-23 21.402531210212477 26.833425290894645 31.13631219011325 20.62773130877962 24-25 21.32313345052803 27.00059967850012 31.264534431874818 20.411732439097033 26-27 21.087086442680057 27.12697365386428 31.311230148145626 20.474709755310037 28-29 20.8075212226875 27.185545134480332 31.4499109480616 20.557022694770563 30-31 21.007636993984914 26.96619693241768 31.355193544465155 20.670972529132257 32-33 20.9510843134286 27.23779088248182 31.18951594708338 20.621608857006198 34-35 21.352696313252927 27.085811861943387 31.06171016092701 20.499781663876675 36-37 21.379028354067216 26.870217487059655 30.957353703958578 20.793400454914547 38-39 20.8986502070819 27.432961622158263 31.030520763345 20.637867407414838 40-41 21.20239266258163 27.24950090193613 31.01850598466458 20.529600450817657 42-43 21.060161002210435 27.380101883506274 30.61704144776653 20.942695666516762 44-45 21.267743256772807 27.33452954456238 30.341677232530728 21.05604996613409 46-47 21.60086993664984 27.64788722508658 29.789163693681225 20.96207914458235 48-49 21.563198463017873 27.962210173883133 29.46364557962215 21.01094578347684 50-51 21.614559569853224 28.054301818717843 29.251329417863886 21.079809193565048 52-53 21.678220182078793 28.284680224061493 29.011406119728413 21.025693474131305 54-55 21.650024667193435 28.166826435209515 28.92769905221824 21.255449845378813 56-57 21.608521034731453 28.45862455450399 28.78554097385045 21.147313436914107 58-59 21.85708627665281 28.032417039751735 28.99239457944581 21.118102104149642 60-61 21.676844607028556 28.25325386175565 29.2304715025608 20.83943002865499 62-63 21.513613734490878 28.170005537696824 29.406523853083826 20.909856874728476 64-65 21.551580217092788 28.169145069940253 29.166948673714586 21.112326039252373 66-67 21.54483650693379 28.355192571869768 28.807969597609524 21.29200132358692 68-69 21.479970380337917 28.185931061590146 29.08058723938031 21.253511318691622 70-71 21.689180652678317 28.185531822730436 28.98596318481991 21.139324339771342 72-73 21.65648683891908 28.11705523477776 28.939864065082716 21.286593861220446 74-75 21.554011744528186 28.66146670737595 28.545129399873964 21.239392148221903 76-77 21.604747945860662 28.287888837584497 28.738724104859497 21.368639111695344 78-79 21.585746272173687 28.278887833814803 28.5650112253421 21.570354668669413 80-81 21.654305309438357 28.628422038720245 28.265866234631236 21.45140641721016 82-83 21.79711399175985 28.484777983745335 28.422051754404 21.296056270090823 84-85 21.49681065594895 28.47235383719053 28.35756051530895 21.673274991551573 86-87 21.344017487719615 28.566726454813573 28.493827531877614 21.595428525589202 88-89 21.57934052932123 28.45615477557241 28.33984247705088 21.62466221805548 90-91 21.488205132061662 28.378147689970636 28.400156922317187 21.73349025565052 92-93 21.500203045176296 28.301063355108468 28.454099256503714 21.744634343211523 94-95 21.399532745421457 28.101377197776028 28.519148914836336 21.97994114196618 96-97 21.428730788889798 28.219453554542646 28.48036041645829 21.871455240109267 98-99 21.261793466776 28.343225510813124 28.315375168675054 22.079605853735817 100-101 20.934667151636813 28.70916305299569 28.198189684324042 22.157980111043457 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 745.0 1 530.0 2 357.0 3 418.0 4 380.5 5 378.0 6 645.5 7 1410.5 8 2403.5 9 2900.5 10 2782.0 11 2622.0 12 2808.5 13 3088.0 14 3265.0 15 3507.0 16 3801.5 17 4054.0 18 4302.5 19 4607.5 20 5024.0 21 5580.5 22 6435.0 23 7765.5 24 9271.5 25 11339.0 26 14538.0 27 17864.5 28 20379.5 29 23830.0 30 28551.0 31 32936.5 32 37967.0 33 43827.5 34 50377.5 35 55738.0 36 62832.0 37 69436.0 38 70483.5 39 70427.5 40 69797.5 41 69207.5 42 68307.0 43 69424.5 44 72280.5 45 73215.0 46 71760.0 47 71665.0 48 75916.0 49 74036.5 50 66900.0 51 61892.0 52 58076.5 53 55457.0 54 52424.5 55 47673.5 56 42062.0 57 36751.0 58 32799.0 59 29574.0 60 25416.0 61 20648.5 62 16011.5 63 12024.0 64 8800.0 65 6469.0 66 4815.5 67 3730.5 68 2735.5 69 1743.5 70 1139.5 71 767.0 72 559.5 73 435.0 74 314.0 75 212.5 76 146.0 77 84.0 78 46.0 79 25.5 80 20.0 81 13.0 82 7.5 83 3.0 84 2.0 85 2.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028526579602944104 2 0.006316957873411172 3 0.0 4 3.509421040783985E-4 5 5.013458629691407E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.013636607472760626 12-13 0.0 14-15 0.0 16-17 2.5067293148457036E-5 18-19 3.008075177814844E-4 20-21 8.27220673899082E-4 22-23 0.0025819311942910745 24-25 0.01148082026199332 26-27 0.005364400733769805 28-29 4.2614398352376956E-4 30-31 0.0031835462298540436 32-33 0.0048630548708006646 34-35 0.0 36-37 0.00955063868956213 38-39 0.012583781160525432 40-41 0.001654441347798164 42-43 0.0 44-45 0.0 46-47 1.504037588907422E-4 48-49 0.003659824799674727 50-51 0.007470053358240196 52-53 0.0 54-55 0.003960632317456211 56-57 0.0042363725420892385 58-59 5.013458629691407E-5 60-61 0.0019803161587281055 62-63 0.005991083062481231 64-65 0.0 66-67 0.0025568639011426175 68-69 5.013458629691406E-4 70-71 9.525571396413672E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0018549796929858203 80-81 0.0020805853313219337 82-83 0.012533646574228516 84-85 0.00887382177455379 86-87 0.004436910887276895 88-89 5.013458629691407E-5 90-91 6.768169150083399E-4 92-93 5.013458629691407E-5 94-95 5.013458629691407E-5 96-97 0.0024816620216972464 98-99 0.0020805853313219337 100-101 3.2587481092994145E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1994631.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.39660039598619 #Duplication Level Percentage of deduplicated Percentage of total 1 76.58622481928916 40.89444042512742 2 13.333102165491189 14.238846567391825 3 4.3634217804498725 6.989756675094734 4 1.866352614931593 3.986275391100246 5 0.97506647287234 2.603261740574402 6 0.6023985708546491 1.9299621460223324 7 0.40086651412102653 1.4983436346656687 8 0.28394375290432133 1.2129304887014951 9 0.21140861247487477 1.0159651080531709 >10 1.1957130422504847 11.936545292092372 >50 0.11157473766192645 4.133165677575389 >100 0.06578127356126788 6.445171278848213 >500 0.0032034515151819227 1.145605128509492 >1k 6.595341354786305E-4 0.6054787985425202 >5k 1.8843832442246586E-4 0.7858898472814879 >10k+ 9.421916221123293E-5 0.5783618004191783 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11496 0.576347204069324 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9367 0.469610669843194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6254 0.3135417027009006 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3235 0.162185386670517 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2539 0.12729171460786481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.0134586296914065E-5 0.0 5 0.0 0.0 0.0 5.0134586296914065E-5 0.0 6 0.0 0.0 0.0 5.0134586296914065E-5 0.0 7 0.0 0.0 0.0 5.0134586296914065E-5 0.0 8 0.0 0.0 0.0 5.0134586296914065E-5 5.0134586296914065E-5 9 0.0 0.0 0.0 1.0026917259382813E-4 5.0134586296914065E-5 10-11 0.0 0.0 0.0 1.504037588907422E-4 5.0134586296914065E-5 12-13 0.0 0.0 0.0 2.2560563833611328E-4 5.0134586296914065E-5 14-15 0.0 0.0 0.0 2.506729314845703E-4 5.0134586296914065E-5 16-17 0.0 0.0 0.0 2.506729314845703E-4 5.0134586296914065E-5 18-19 0.0 0.0 0.0 3.2587481092994145E-4 5.0134586296914065E-5 20-21 0.0 0.0 0.0 4.762785698206836E-4 5.0134586296914065E-5 22-23 0.0 0.0 0.0 7.770860876021681E-4 5.0134586296914065E-5 24-25 0.0 0.0 0.0 0.0011029608985321094 5.0134586296914065E-5 26-27 0.0 0.0 0.0 0.0014539030026105078 1.0026917259382813E-4 28-29 0.0 0.0 0.0 0.0026571330737364457 1.0026917259382813E-4 30-31 0.0 0.0 0.0 0.00523906426802752 1.0026917259382813E-4 32-33 0.0 0.0 0.0 0.00844767779103002 1.0026917259382813E-4 34-35 0.0 0.0 0.0 0.012884588678306915 1.0026917259382813E-4 36-37 0.0 0.0 0.0 0.0165694807711301 1.0026917259382813E-4 38-39 0.0 0.0 0.0 0.021106660831000823 1.0026917259382813E-4 40-41 0.0 0.0 0.0 0.02830097396460799 1.0026917259382813E-4 42-43 0.0 0.0 0.0 0.03699932468712258 1.0026917259382813E-4 44-45 0.0 0.0 0.0 0.04529659871926186 1.0026917259382813E-4 46-47 0.0 0.0 0.0 0.05464669906363633 1.0026917259382813E-4 48-49 0.0 0.0 0.0 0.06414720316690155 1.0026917259382813E-4 50-51 0.0 0.0 0.0 0.07610430199871555 1.0026917259382813E-4 52-53 0.0 0.0 0.0 0.10335244965108834 1.0026917259382813E-4 54-55 0.0 0.0 0.0 0.14090325478747698 1.0026917259382813E-4 56-57 0.0 0.0 0.0 0.17599746519531684 1.0026917259382813E-4 58-59 0.0 0.0 0.0 0.2340031815408464 1.0026917259382813E-4 60-61 0.0 0.0 0.0 0.3033142470963301 1.0026917259382813E-4 62-63 0.0 0.0 0.0 0.3495634029552333 1.0026917259382813E-4 64-65 0.0 0.0 0.0 0.38653766034920745 1.0026917259382813E-4 66-67 0.0 0.0 0.0 0.4852777280609797 1.0026917259382813E-4 68-69 0.0 0.0 0.0 0.5923150698048911 1.0026917259382813E-4 70-71 0.0 0.0 0.0 0.6408202820471556 1.0026917259382813E-4 72-73 0.0 0.0 0.0 0.7367277456331522 1.0026917259382813E-4 74-75 0.0 0.0 0.0 0.8810401522888194 1.0026917259382813E-4 76-77 0.0 0.0 0.0 1.0181833131040279 1.0026917259382813E-4 78-79 0.0 0.0 0.0 1.151591447240116 1.0026917259382813E-4 80-81 0.0 0.0 0.0 1.3132002861682186 1.0026917259382813E-4 82-83 0.0 0.0 0.0 1.4613479886755996 1.0026917259382813E-4 84-85 0.0 0.0 0.0 1.6254384896253993 1.0026917259382813E-4 86-87 0.0 0.0 0.0 1.9035350398143818 1.0026917259382813E-4 88-89 0.0 0.0 0.0 2.273177344581529 1.0026917259382813E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8800 0.0 34.06643 1 GTATCAA 13230 0.0 30.918844 1 ATCAACG 16365 0.0 24.930237 3 TCAACGC 16500 0.0 24.697477 4 TATCAAC 16660 0.0 24.434229 2 CAACGCA 16700 0.0 24.40537 5 AACGCAG 17035 0.0 23.953314 6 GTACATG 8660 0.0 21.834595 1 GAGTACT 10695 0.0 21.64872 12-13 ACGCAGA 19045 0.0 21.300583 7 AGAGTAC 15565 0.0 20.96632 10-11 CGCAGAG 19105 0.0 20.960186 8 AGTACTT 11250 0.0 20.559607 12-13 GTACTTT 11345 0.0 20.158209 14-15 ACATGGG 8805 0.0 20.066093 3 TACATGG 9110 0.0 20.021917 2 GCAGAGT 20075 0.0 19.568815 9 GTATAGT 650 0.0 18.272861 1 GTATAGG 950 0.0 18.003578 1 TACTTTT 12695 0.0 17.995857 14-15 >>END_MODULE