FastQCFastQC Report
Thu 26 May 2016
SRR937048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917432
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91570.4775658276277855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66500.3468180357895352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43180.22519703436679894No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24830.12949611772412267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA111800.033.515121
GGTATCA72300.030.7139021
TCAACGC133650.027.9698054
ATCAACG134550.027.7459183
CAACGCA135000.027.7224375
AACGCAG138500.027.1220426
TATCAAC139150.026.7603442
ACGCAGA156650.023.7299397
CGCAGAG157050.023.3970878
GTACATG89650.023.0194991
GAGTACT76600.022.71082512-13
TACATGG91800.022.0688692
GCAGAGT165700.021.716699
AGAGTAC127700.021.27188310-11
ACATGGG89100.021.243133
AGTACTT81950.021.19917712-13
GTACTTT83400.020.77307114-15
CATTCCG3707.2759576E-1219.2712469
CATGGGA57100.018.4073814
GTACCGT1300.00751970518.288236
Produced by FastQC (version 0.11.5)