##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937048_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1917432 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76349982685175 33.0 31.0 34.0 30.0 34.0 2 31.85132771331656 33.0 31.0 34.0 30.0 34.0 3 31.870749001789893 33.0 31.0 34.0 30.0 34.0 4 35.46331186712228 37.0 35.0 37.0 33.0 37.0 5 35.464556761334954 37.0 35.0 37.0 33.0 37.0 6 35.53409560286884 37.0 35.0 37.0 33.0 37.0 7 35.47925089390393 37.0 35.0 37.0 33.0 37.0 8 35.44648832396664 37.0 35.0 37.0 33.0 37.0 9 37.074746848910415 39.0 37.0 39.0 33.0 39.0 10-11 36.94045812315639 39.0 37.0 39.0 33.0 39.0 12-13 36.864507580972884 39.0 37.0 39.0 33.0 39.0 14-15 38.01177434193234 40.0 38.0 41.0 33.0 41.0 16-17 37.953395739718545 40.0 38.0 41.0 33.0 41.0 18-19 37.929399060827194 40.0 38.0 41.0 33.0 41.0 20-21 37.870755520925904 40.0 38.0 41.0 33.0 41.0 22-23 37.75634103321526 40.0 37.5 41.0 32.0 41.0 24-25 37.72229862649627 40.0 37.5 41.0 32.0 41.0 26-27 37.74712349642647 40.0 37.0 41.0 32.0 41.0 28-29 37.68766193533851 40.0 37.0 41.0 32.0 41.0 30-31 37.52763227066201 40.0 37.0 41.0 32.0 41.0 32-33 37.336147513966594 40.0 37.0 41.0 32.0 41.0 34-35 37.17556085430931 40.0 36.0 41.0 31.0 41.0 36-37 37.00280427154652 39.5 36.0 41.0 31.0 41.0 38-39 36.84936075959929 39.0 36.0 41.0 30.5 41.0 40-41 36.67874323574448 39.0 36.0 41.0 30.0 41.0 42-43 36.46261692722349 39.0 35.0 41.0 30.0 41.0 44-45 36.198258399776364 39.0 35.0 41.0 30.0 41.0 46-47 35.84351126923927 39.0 35.0 40.5 28.5 41.0 48-49 35.58954450535926 39.0 35.0 40.5 27.0 41.0 50-51 34.90415279394523 38.0 34.0 40.0 26.0 40.5 52-53 35.08890641232649 38.5 35.0 40.0 27.0 40.5 54-55 35.49253871845259 39.0 35.0 40.5 27.0 41.0 56-57 35.38111651417104 39.0 35.0 41.0 26.5 41.0 58-59 35.18101711038514 39.0 35.0 41.0 26.0 41.0 60-61 34.91527939452351 38.5 34.5 40.0 26.0 41.0 62-63 34.620832968261716 38.0 34.0 40.0 26.0 41.0 64-65 34.310348945881785 37.5 34.0 40.0 25.0 41.0 66-67 33.94445331046941 37.0 34.0 40.0 23.5 41.0 68-69 33.5560671773497 36.5 33.5 39.0 22.5 41.0 70-71 33.15081969008549 36.0 33.0 39.0 22.0 41.0 72-73 32.75772960918562 35.5 33.0 39.0 21.5 40.0 74-75 32.35976269301858 35.0 32.0 38.0 20.5 40.0 76-77 31.90606994146337 35.0 32.0 37.0 18.5 39.0 78-79 31.43721837332432 35.0 31.5 37.0 17.5 39.0 80-81 30.999036471697565 35.0 31.0 36.0 13.0 38.5 82-83 30.585274471271994 35.0 31.0 36.0 9.0 37.0 84-85 30.198653980949523 34.0 30.5 35.0 7.0 37.0 86-87 29.84423671869459 34.0 30.0 35.0 3.5 36.5 88-89 29.480994632404176 34.0 30.0 35.0 2.0 36.0 90-91 29.112278036457095 34.0 29.0 35.0 2.0 36.0 92-93 28.7327866125109 34.0 29.0 35.0 2.0 35.5 94-95 28.36696998902699 34.0 29.0 35.0 2.0 35.0 96-97 27.885668435699415 34.0 29.0 35.0 2.0 35.0 98-99 27.33056635124479 34.0 27.5 35.0 2.0 35.0 100-101 25.1185400577439 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28660672229194617 1101 2 0.2385184527221078 1101 3 0.34964598216681964 1101 4 0.3033107053557629 1101 5 0.26960297899275076 1101 6 0.37075805321011046 1101 7 0.26174285343781634 1101 8 0.24300396835977978 1101 9 0.2302373632453225 1101 10-11 0.2603441628563772 1101 12-13 0.40320259109025613 1101 14-15 0.5414259954465521 1101 16-17 0.5824203212953378 1101 18-19 0.4985821051631021 1101 20-21 0.4941032531349663 1101 22-23 0.5803716625126398 1101 24-25 0.5309154963364193 1101 26-27 0.4667258057306398 1101 28-29 0.431785321387288 1101 30-31 0.4654874611472408 1101 32-33 0.2991699289724181 1101 34-35 0.39346470826681923 1101 36-37 0.5393464042843448 1101 38-39 0.3337216952054476 1101 40-41 0.4124149107485522 1101 42-43 0.43847099292518266 1101 44-45 0.46431680033366973 1101 46-47 0.4652610698033186 1101 48-49 0.4797979636682328 1101 50-51 0.6085125016586872 1101 52-53 0.5567015188687137 1101 54-55 0.5384316813761245 1101 56-57 0.4874554191868796 1101 58-59 0.6517128048116803 1101 60-61 0.6308949535185562 1101 62-63 0.6416477979479822 1101 64-65 0.4669388815733484 1101 66-67 0.49972942496435024 1101 68-69 0.5164848881422301 1101 70-71 0.446628894238863 1101 72-73 0.37745188455134127 1101 74-75 0.34855968555031325 1101 76-77 0.37665744036311466 1101 78-79 0.41430274312840254 1101 80-81 0.4992427145127678 1101 82-83 0.38958494399321 1101 84-85 0.3299379028419942 1101 86-87 0.4558370455701457 1101 88-89 0.46147549734421744 1101 90-91 0.5543850560590435 1101 92-93 0.4152040928592733 1101 94-95 0.5981020098754293 1101 96-97 0.5579095780445265 1101 98-99 0.561876178199789 1101 100-101 0.9376774870573747 1102 1 0.12228715404379642 1102 2 0.053604664767540555 1102 3 0.051849181768780284 1102 4 0.15842509470073907 1102 5 0.11874068541666105 1102 6 0.0037355693662632916 1102 7 -0.04801648825625904 1102 8 0.0021313376827052366 1102 9 0.03178389465541187 1102 10-11 0.03741326666209943 1102 12-13 -0.06503726773933494 1102 14-15 -0.13189374090283934 1102 16-17 0.11304062872152087 1102 18-19 -0.18636890397235817 1102 20-21 -0.21582238933290654 1102 22-23 -0.15987998036968776 1102 24-25 -0.3069556183133031 1102 26-27 -0.1246505487091838 1102 28-29 -0.23351610698851744 1102 30-31 -0.2025291225334982 1102 32-33 -0.15225945959091547 1102 34-35 -0.2851608641386747 1102 36-37 -0.07435763816095431 1102 38-39 -0.10746312596236152 1102 40-41 -0.262165931146086 1102 42-43 -0.12273248730716801 1102 44-45 -0.08638355513726736 1102 46-47 0.050950374045967806 1102 48-49 0.20468868487795078 1102 50-51 0.2173905150378843 1102 52-53 0.31861095506130965 1102 54-55 -0.049273019970641485 1102 56-57 0.1527742463007229 1102 58-59 -0.08276055269983829 1102 60-61 -0.09050918177194944 1102 62-63 -0.0611067079356431 1102 64-65 0.04172134108319625 1102 66-67 -0.19145405450972675 1102 68-69 0.011072755318359384 1102 70-71 0.11732462389606013 1102 72-73 0.14101671112401704 1102 74-75 0.2864556258620752 1102 76-77 -0.025558622186476754 1102 78-79 0.06004236348274361 1102 80-81 0.012043165183364124 1102 82-83 0.027658732300945843 1102 84-85 -0.028606201920222674 1102 86-87 0.10056814504401146 1102 88-89 0.1431575346273739 1102 90-91 6.330831200571652E-4 1102 92-93 0.1507331469340336 1102 94-95 0.11759933003696688 1102 96-97 -0.159529722432719 1102 98-99 -0.1123663837531339 1102 100-101 -0.2034502558623359 1103 1 0.23955658756093 1103 2 0.17517726855774995 1103 3 0.25843885983346127 1103 4 0.1612221648972607 1103 5 0.12584111626674854 1103 6 0.1786680176079969 1103 7 0.17327741753090464 1103 8 0.20744679911076247 1103 9 0.07043322882819325 1103 10-11 0.1343341668039173 1103 12-13 0.10307613202029842 1103 14-15 0.3050039785102001 1103 16-17 0.24446637490358114 1103 18-19 0.18255041448831832 1103 20-21 0.16325230055154094 1103 22-23 0.3315149165573601 1103 24-25 0.2814606044653871 1103 26-27 0.31426786925177197 1103 28-29 0.3542605325254584 1103 30-31 0.2509103771160994 1103 32-33 0.1739007810043418 1103 34-35 0.30250335569444076 1103 36-37 0.20078519956309293 1103 38-39 0.2464520203098246 1103 40-41 0.24607107124451488 1103 42-43 0.2623378554291236 1103 44-45 0.20648246896985256 1103 46-47 0.13179954338732358 1103 48-49 -0.07990692506211872 1103 50-51 -0.0014217810289096633 1103 52-53 -0.030553912351059864 1103 54-55 0.10491956779333833 1103 56-57 0.032428454404502816 1103 58-59 0.07400674084536263 1103 60-61 0.13401405312007597 1103 62-63 0.13924981761274324 1103 64-65 0.10106246465935698 1103 66-67 0.13945979660396546 1103 68-69 0.1762324017360939 1103 70-71 0.09606159782477874 1103 72-73 0.017746960432980075 1103 74-75 0.0799244897988558 1103 76-77 0.07670349786403463 1103 78-79 0.11828023210459193 1103 80-81 -0.02004375341127229 1103 82-83 -0.03323246924555079 1103 84-85 0.11513221223693293 1103 86-87 0.1532351948831483 1103 88-89 -0.024550925824598835 1103 90-91 0.19020284034114 1103 92-93 0.05148979516361507 1103 94-95 0.2243341541891688 1103 96-97 0.2370526431172486 1103 98-99 0.2516626309954546 1103 100-101 0.6504802160395009 1104 1 0.1706714998399228 1104 2 0.08050133616541189 1104 3 0.114145343264493 1104 4 0.11861624126868975 1104 5 0.29514784777703795 1104 6 0.19578388400466906 1104 7 0.11092823081553149 1104 8 0.03262758015764433 1104 9 0.17899107540667814 1104 10-11 0.2173495511394563 1104 12-13 0.11809146698251283 1104 14-15 0.06285536439536799 1104 16-17 0.2558369630025794 1104 18-19 0.3143696977566819 1104 20-21 0.25100579084438124 1104 22-23 0.1827103463206683 1104 24-25 0.2252788595560844 1104 26-27 0.22078739910519118 1104 28-29 0.04220406677569599 1104 30-31 0.37543117984825614 1104 32-33 0.17055324851160236 1104 34-35 0.2733147390156532 1104 36-37 0.2607888289349276 1104 38-39 0.2834173673880045 1104 40-41 0.2534307270518781 1104 42-43 0.37095676900394636 1104 44-45 0.29451617448704326 1104 46-47 0.3178607199577783 1104 48-49 0.4947730308223939 1104 50-51 0.3088162091826092 1104 52-53 0.45895566553091527 1104 54-55 0.6140091303392623 1104 56-57 0.6631257940273017 1104 58-59 0.5186592533949579 1104 60-61 0.47074136884229034 1104 62-63 0.43062925826071563 1104 64-65 0.4380627411932423 1104 66-67 0.21685106216116168 1104 68-69 0.17824739606573559 1104 70-71 0.1861324940089375 1104 72-73 0.2705041244903299 1104 74-75 0.3862818023226353 1104 76-77 0.42482825383966727 1104 78-79 0.5206910575073387 1104 80-81 0.446235679202708 1104 82-83 0.4253386626528801 1104 84-85 0.41002311344937326 1104 86-87 0.46817675138560233 1104 88-89 0.41930262685619013 1104 90-91 0.3675579637646855 1104 92-93 0.5145946101663483 1104 94-95 0.5203582596280505 1104 96-97 0.5591705461632124 1104 98-99 0.4954005028626014 1104 100-101 0.25114872640974184 1105 1 0.10914841276920839 1105 2 0.2004007019502545 1105 3 0.2488008930648249 1105 4 0.13453407893793212 1105 5 0.24616425494141936 1105 6 0.23135833367125258 1105 7 0.12792895247297764 1105 8 0.13714776548118834 1105 9 0.1301308088151245 1105 10-11 0.13838972446453823 1105 12-13 0.2129388965055412 1105 14-15 0.3649227100724559 1105 16-17 0.33378670795594445 1105 18-19 0.2297249290570349 1105 20-21 0.3527658552604649 1105 22-23 0.41586696508106513 1105 24-25 0.41986062047980255 1105 26-27 0.3771654744042152 1105 28-29 0.40799845885290154 1105 30-31 0.4706903598958263 1105 32-33 0.256890769367665 1105 34-35 0.22534600182069653 1105 36-37 0.20164394924167084 1105 38-39 0.4364131209869342 1105 40-41 0.47455746744375915 1105 42-43 0.5746503502309892 1105 44-45 0.3088526514461165 1105 46-47 0.4588795061251858 1105 48-49 0.554131543396494 1105 50-51 0.4154767015805447 1105 52-53 0.4143489608797566 1105 54-55 0.40052105837401797 1105 56-57 0.5519969133283311 1105 58-59 0.41314887767957487 1105 60-61 0.47886180527397926 1105 62-63 0.4695474404477906 1105 64-65 0.48617588944173207 1105 66-67 0.556267959766167 1105 68-69 0.6048197460035638 1105 70-71 0.3743561903112962 1105 72-73 0.42807369043764965 1105 74-75 0.5625114246499407 1105 76-77 0.6543328244694031 1105 78-79 0.7089215168617891 1105 80-81 0.5995166744693634 1105 82-83 0.39118326044621554 1105 84-85 0.583607984581402 1105 86-87 0.688581436509832 1105 88-89 0.6993984075869015 1105 90-91 0.6240654122720031 1105 92-93 0.7904213137194986 1105 94-95 0.8656924299885382 1105 96-97 0.7209817392531228 1105 98-99 0.5869232955416379 1105 100-101 0.6546041686377606 1106 1 0.24100707264871346 1106 2 0.2649299815020818 1106 3 0.3066398857823813 1106 4 0.27437214986059644 1106 5 0.15717434773588224 1106 6 0.2897279866491971 1106 7 0.30167571809042215 1106 8 0.30323847565340145 1106 9 0.22522479684765528 1106 10-11 0.43256677785525 1106 12-13 0.3072299509291341 1106 14-15 0.47020405258671616 1106 16-17 0.4521042735710097 1106 18-19 0.4788120611573916 1106 20-21 0.3406276217342423 1106 22-23 0.20152386297012725 1106 24-25 0.21652946268822149 1106 26-27 0.17044321570254795 1106 28-29 0.23917575757066345 1106 30-31 0.48647989970592675 1106 32-33 0.44891307759763066 1106 34-35 0.4661164808432545 1106 36-37 0.18875667470615554 1106 38-39 0.02383319958416763 1106 40-41 0.19890938413572457 1106 42-43 0.2798183199083013 1106 44-45 0.15176613828408847 1106 46-47 0.18014499694129427 1106 48-49 0.21313736049096121 1106 50-51 0.26338285836369835 1106 52-53 0.2712393115916356 1106 54-55 0.3529765440785937 1106 56-57 0.33121083520853034 1106 58-59 0.31870044363981265 1106 60-61 0.31747760526167923 1106 62-63 0.2681433563806266 1106 64-65 0.06410789483495449 1106 66-67 0.15181524076879782 1106 68-69 0.3645091725400107 1106 70-71 0.3589422455515461 1106 72-73 0.3428823849340574 1106 74-75 0.43953694329181303 1106 76-77 0.45069203220189635 1106 78-79 0.4979339729955292 1106 80-81 0.4058848620976079 1106 82-83 0.41234585937881363 1106 84-85 0.4832047489093938 1106 86-87 0.6971239094990587 1106 88-89 0.5656148230743767 1106 90-91 0.672468075293807 1106 92-93 0.5815283073919097 1106 94-95 0.6978140064032878 1106 96-97 0.7640525065184285 1106 98-99 0.6891798745716642 1106 100-101 0.9168819277900795 1107 1 0.27069210789570164 1107 2 0.3560215254970025 1107 3 0.3369180981021387 1107 4 0.36644550418284894 1107 5 0.2560050116360202 1107 6 0.3525924837332184 1107 7 0.4008172885942187 1107 8 0.4568181561068627 1107 9 0.4120166661490643 1107 10-11 0.45637526920550187 1107 12-13 0.5915380855549657 1107 14-15 0.5422251582360502 1107 16-17 0.5798791150086444 1107 18-19 0.6042088761646838 1107 20-21 0.7037493531240244 1107 22-23 0.6356096175568169 1107 24-25 0.6027421457140392 1107 26-27 0.6725156536684977 1107 28-29 0.6856482766995953 1107 30-31 0.7512512360806838 1107 32-33 0.6184721302161122 1107 34-35 0.5165872588952141 1107 36-37 0.5825243882008166 1107 38-39 0.8478690618427223 1107 40-41 0.9446892360787302 1107 42-43 0.9555979591257326 1107 44-45 0.9675040669261179 1107 46-47 0.8567329933282295 1107 48-49 0.7694969550687532 1107 50-51 0.6484202326101638 1107 52-53 0.7449198403292243 1107 54-55 0.753538794126051 1107 56-57 0.6917524508756046 1107 58-59 0.732150999671731 1107 60-61 0.8910511984911125 1107 62-63 0.9455573007054383 1107 64-65 0.9645637820969526 1107 66-67 0.828280919952455 1107 68-69 0.8076541728345532 1107 70-71 0.809251188534553 1107 72-73 0.6652008972950441 1107 74-75 0.8598286718670778 1107 76-77 0.901687015151122 1107 78-79 0.976957186264368 1107 80-81 1.0048587066704862 1107 82-83 1.0648561847507558 1107 84-85 1.0261924959165079 1107 86-87 1.120505067904336 1107 88-89 0.9665644791134902 1107 90-91 0.9119172960636774 1107 92-93 1.036442758985391 1107 94-95 1.2217718147408192 1107 96-97 1.2698803689864384 1107 98-99 1.4630260019844634 1107 100-101 1.1857993673596532 1108 1 -0.028850244837531847 1108 2 0.08891693724091354 1108 3 0.0660064252376209 1108 4 0.02924689116078838 1108 5 -0.16451494913211917 1108 6 0.09307607185414213 1108 7 0.046618925103416586 1108 8 -4.2968735385784385E-4 1108 9 -0.12149720189741231 1108 10-11 -0.1463581811533814 1108 12-13 0.06520928826099492 1108 14-15 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-3.1813549977377704 2316 82-83 -3.793683292203898 2316 84-85 -3.676271937541266 2316 86-87 -3.8678529034061775 2316 88-89 -3.4430283052035158 2316 90-91 -3.9834288763340737 2316 92-93 -4.228316956137743 2316 94-95 -4.410432240374096 2316 96-97 -4.616865018621812 2316 98-99 -5.0008105272722645 2316 100-101 -5.158748049774797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4123.0 3 1469.0 4 1363.0 5 1503.0 6 1684.0 7 1955.0 8 2488.0 9 2900.0 10 3722.0 11 4770.0 12 6283.0 13 7680.0 14 8941.0 15 11202.0 16 14499.0 17 19071.0 18 16668.0 19 10226.0 20 9875.0 21 10580.0 22 11505.0 23 12625.0 24 14478.0 25 16516.0 26 19267.0 27 22687.0 28 27248.0 29 32635.0 30 40363.0 31 50225.0 32 64234.0 33 83932.0 34 115230.0 35 165062.0 36 250601.0 37 387136.0 38 386965.0 39 75673.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.008733670019616 19.6821653658333 14.832161192036395 22.476939772110686 2 14.817067699453906 20.783834598233383 41.263112426208366 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30.528495365291043 20.66573764627701 22-23 21.34447420200424 27.097279743988178 30.462365005179244 21.09588104882834 24-25 21.13778754636835 27.32866659434108 30.53063857930292 21.00290727998765 26-27 20.951010772305093 27.39754783960638 30.566549344532 21.08489204355653 28-29 20.721206341308342 27.42362116756285 30.725762223625235 21.129410267503573 30-31 20.94855073616485 27.23253181302907 30.672592251069258 21.146325199736822 32-33 20.73967482138438 27.47033547686857 30.546511515710378 21.243478186036675 34-35 21.203087871119298 27.455030699292966 30.292029240998314 21.04985218858942 36-37 21.27098895436229 27.13650004850526 30.281257464854548 21.311253532277902 38-39 20.818471372460255 27.595395143444108 30.392903393038473 21.193230091057163 40-41 20.990592177751118 27.48265453146698 30.40962818565291 21.11712510512899 42-43 20.933935588652766 27.41572541455036 30.076822236541766 21.573516760255114 44-45 21.037734668204873 27.492401120691273 29.927901916565663 21.541962294538195 46-47 21.271737106635495 27.73582573494016 29.50311385046738 21.48932330795697 48-49 21.187851023427726 27.951465217225774 29.34744911024085 21.51323464910565 50-51 21.170674414001738 28.04947661939495 29.197964629531647 21.58188433707166 52-53 21.30148875091795 28.26214512817945 28.895949120435276 21.540417000467322 54-55 21.286986565560863 28.05201023680634 28.97737037799144 21.683632819641357 56-57 21.317608597403662 28.35303097909978 28.68326503767193 21.646095385824633 58-59 21.458045117487753 28.016288297121903 28.916291035308262 21.60937555008208 60-61 21.41028947476161 28.055063493979958 29.190228469846847 21.344418561411587 62-63 21.175917720809252 28.20283662715403 29.26861898411132 21.352626667925396 64-65 21.161123697704788 28.119641136047864 29.090312634297565 21.628922531949783 66-67 21.163726390808122 28.355052198829732 28.62289299357276 21.858328416789384 68-69 21.077264676578757 28.21032183636365 29.051051930071164 21.661361556986428 70-71 21.304452873825934 28.047323166935673 28.997167999173413 21.65105596006498 72-73 21.18167226679288 28.052107903153672 28.959836859652622 21.806382970400822 74-75 21.043938590190766 28.565299691782553 28.680705191500106 21.71005652652657 76-77 21.11981567060667 28.237123259588014 28.79630339649964 21.846757673305675 78-79 21.10841554575808 28.180540336667736 28.71840430645909 21.99263981111509 80-81 21.15859617715715 28.42124079943319 28.50694095588034 21.913222067529322 82-83 21.245202543062998 28.281037987877305 28.724879375617206 21.748880093442487 84-85 21.122363450205007 28.295155878451787 28.543378445268203 22.039102226075 86-87 21.016682913010804 28.382028456708603 28.6813489795583 21.919939650722295 88-89 21.159998100733148 28.161666220547666 28.575200270906414 22.103135407812772 90-91 21.08380365134083 28.04233618611184 28.798488582190124 22.075371580357203 92-93 21.175084882169333 28.065443076445472 28.76430083571273 21.995171205672463 94-95 20.960750962722567 27.86369214993175 28.815304876490377 22.360252010855305 96-97 20.92445357845637 27.92016201279776 28.948129563293097 22.207254845452777 98-99 20.817725071997998 28.05545410910305 28.75837368003673 22.368447138862223 100-101 20.68788423327667 28.106656033352973 28.88150309683083 22.323956636539524 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 372.0 1 293.5 2 245.5 3 304.5 4 323.5 5 358.0 6 594.0 7 1176.5 8 2029.5 9 2522.0 10 2357.0 11 2150.5 12 2270.0 13 2484.0 14 2685.5 15 2867.5 16 2980.5 17 3132.0 18 3306.5 19 3552.5 20 4042.0 21 4692.0 22 5589.5 23 6893.0 24 8360.5 25 10517.5 26 13709.5 27 16907.5 28 19358.5 29 22537.0 30 27067.0 31 31727.5 32 36854.0 33 42515.0 34 48928.5 35 54686.0 36 62078.0 37 68239.0 38 68653.5 39 68083.0 40 67232.0 41 66772.0 42 66261.0 43 67726.0 44 70560.0 45 71429.0 46 69790.0 47 69086.0 48 73174.0 49 71887.5 50 64983.5 51 59842.5 52 55831.0 53 53175.5 54 50378.5 55 45941.0 56 40816.5 57 35690.5 58 31674.0 59 28339.0 60 24178.0 61 19364.5 62 14872.5 63 11190.0 64 8303.0 65 6176.5 66 4453.0 67 3380.5 68 2487.0 69 1616.0 70 1078.5 71 742.0 72 524.0 73 381.5 74 273.5 75 195.0 76 123.5 77 69.0 78 44.0 79 26.0 80 24.5 81 18.0 82 9.0 83 6.5 84 5.0 85 5.5 86 8.5 87 8.5 88 6.0 89 4.0 90 2.0 91 1.0 92 1.0 93 1.5 94 1.5 95 1.5 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03713299871912015 2 0.028579892272581245 3 0.023625348904159314 4 0.04954543368421931 5 0.09794349943048827 6 0.1146324876188569 7 0.05679471292854192 8 0.08824302504599903 9 0.07155403685763041 10-11 0.07231025663491586 12-13 0.07048489855181306 14-15 0.06344423165984504 16-17 0.0498322756687069 18-19 0.06177533284100819 20-21 0.056142799327433775 22-23 0.033664818361224806 24-25 0.009178943503602736 26-27 0.0032074149174520917 28-29 0.006805977995569074 30-31 0.0050327730005549085 32-33 0.006075834762327947 34-35 0.006466982922992836 36-37 0.005945452042106317 38-39 0.003389950725762374 40-41 0.0075361212288102 42-43 0.004354782855402434 44-45 0.002294735875900684 46-47 0.001616745730748209 48-49 1.5645926426595573E-4 50-51 0.0021643531556790542 52-53 0.006258370570638229 54-55 0.012125592980611567 56-57 0.008109805197785373 58-59 0.005449997705264124 60-61 0.002372965508033662 62-63 0.008083728653741046 64-65 0.0025033482282552917 66-67 0.02268659331856358 68-69 0.04607725332632396 70-71 0.05835930557120148 72-73 0.0033638741817180475 74-75 0.014550711576733882 76-77 0.022373674790031668 78-79 0.025946161324104322 80-81 0.07559590118450094 82-83 0.02769328977507416 84-85 0.010482770705819034 86-87 0.03079639851634895 88-89 0.04709423854405267 90-91 0.042817685320783214 92-93 0.04195715936732046 94-95 0.011108607762882857 96-97 0.015619849882551247 98-99 0.03713299871912015 100-101 0.0751525999357474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1917432.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.51547667397069 #Duplication Level Percentage of deduplicated Percentage of total 1 78.48660917853503 44.357081302485504 2 12.560140978115058 14.196847089408902 3 3.9058071177260074 6.622156531646185 4 1.654844909254015 3.7409739547193768 5 0.8810186386928244 2.4895594162188868 6 0.528483353947619 1.7920493197565077 7 0.3415993132176878 1.3513953612599054 8 0.23076752398591485 1.0433549295148754 9 0.18389231243485946 0.9353485524541346 >10 1.0608813440843285 11.392085979853036 >50 0.1062648595024725 4.158789067950628 >100 0.056923395593746304 5.731367581523545 >500 0.002029188267709238 0.7200895500455536 >1k 5.534149821025191E-4 0.6449216673782818 >5k 1.844716607008397E-4 0.8239796957847154 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9157 0.4775658276277855 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6650 0.3468180357895352 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4318 0.22519703436679894 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2483 0.12949611772412267 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.2153088088651906E-5 0.0 10-11 0.0 0.0 0.0 1.0430617617730381E-4 0.0 12-13 0.0 0.0 0.0 1.564592642659557E-4 0.0 14-15 0.0 0.0 0.0 1.564592642659557E-4 0.0 16-17 0.0 0.0 0.0 2.3468889639893358E-4 0.0 18-19 0.0 0.0 0.0 2.6076544044325954E-4 0.0 20-21 0.0 0.0 0.0 3.3899507257623737E-4 0.0 22-23 0.0 0.0 0.0 5.997605130194969E-4 0.0 24-25 0.0 0.0 0.0 0.001121291393906016 0.0 26-27 0.0 0.0 0.0 0.0015385160986152312 0.0 28-29 0.0 0.0 0.0 0.002764113668698551 0.0 30-31 0.0 0.0 0.0 0.005449997705264125 0.0 32-33 0.0 0.0 0.0 0.008839948431026498 0.0 34-35 0.0 0.0 0.0 0.01296004239003 0.0 36-37 0.0 0.0 0.0 0.016610758556235634 0.0 38-39 0.0 0.0 0.0 0.02086123523546076 0.0 40-41 0.0 0.0 0.0 0.027171758894187645 0.0 42-43 0.0 0.0 0.0 0.036689697470366614 0.0 44-45 0.0 0.0 0.0 0.045660028621614744 0.0 46-47 0.0 0.0 0.0 0.05622102895956675 0.0 48-49 0.0 0.0 0.0 0.065999732976189 0.0 50-51 0.0 0.0 0.0 0.07979422477563741 0.0 52-53 0.0 0.0 0.0 0.11019947513132147 0.0 54-55 0.0 0.0 0.0 0.14834945906817035 0.0 56-57 0.0 0.0 0.0 0.18527384543493589 0.0 58-59 0.0 0.0 0.0 0.24660587702719053 0.0 60-61 0.0 0.0 0.0 0.32188885968315956 0.0 62-63 0.0 0.0 0.0 0.36794003646543916 0.0 64-65 0.0 0.0 0.0 0.40288260548483595 0.0 66-67 0.0 0.0 0.0 0.5044246679934412 0.0 68-69 0.0 0.0 0.0 0.6162148123114666 2.6076544044325953E-5 70-71 0.0 0.0 0.0 0.6649518731303118 5.2153088088651906E-5 72-73 0.0 0.0 0.0 0.760887478669387 5.2153088088651906E-5 74-75 0.0 0.0 0.0 0.904725695617889 5.2153088088651906E-5 76-77 0.0 0.0 0.0 1.045200038384673 5.2153088088651906E-5 78-79 0.0 0.0 0.0 1.181528210648409 5.2153088088651906E-5 80-81 0.0 0.0 0.0 1.3439329269564708 5.2153088088651906E-5 82-83 0.0 0.0 0.0 1.486988847583643 2.0861235235460762E-4 84-85 0.0 0.0 0.0 1.6465512205908737 2.0861235235460762E-4 86-87 0.0 0.0 0.0 1.934670955736631 2.0861235235460762E-4 88-89 0.0 0.0 0.0 2.335545667330054 2.0861235235460762E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11180 0.0 33.51512 1 GGTATCA 7230 0.0 30.713902 1 TCAACGC 13365 0.0 27.969805 4 ATCAACG 13455 0.0 27.745918 3 CAACGCA 13500 0.0 27.722437 5 AACGCAG 13850 0.0 27.122042 6 TATCAAC 13915 0.0 26.760344 2 ACGCAGA 15665 0.0 23.729939 7 CGCAGAG 15705 0.0 23.397087 8 GTACATG 8965 0.0 23.019499 1 GAGTACT 7660 0.0 22.710825 12-13 TACATGG 9180 0.0 22.068869 2 GCAGAGT 16570 0.0 21.71669 9 AGAGTAC 12770 0.0 21.271883 10-11 ACATGGG 8910 0.0 21.24313 3 AGTACTT 8195 0.0 21.199177 12-13 GTACTTT 8340 0.0 20.773071 14-15 CATTCCG 370 7.2759576E-12 19.271246 9 CATGGGA 5710 0.0 18.407381 4 GTACCGT 130 0.007519705 18.28823 6 >>END_MODULE