FastQCFastQC Report
Thu 26 May 2016
SRR937048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917432
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117410.6123294072488621No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92930.48465864760784216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62090.32381852394243965No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32430.16913246467149815No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25160.13121716963104818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85850.032.3189161
GTATCAA128300.031.327661
TCAACGC160150.024.9425014
ATCAACG160150.024.9128443
TATCAAC161500.024.676472
CAACGCA162450.024.6185975
AACGCAG165950.024.1336616
GTACATG91150.024.1328491
CGTATAG2052.665729E-823.1755661
TACATGG93250.023.0241282
ACATGGG90350.022.7104913
GAGTACT101550.021.68360112-13
ACGCAGA185450.021.4679267
CGCAGAG185100.021.3305238
AGAGTAC150000.021.30138810-11
GTACCGT1353.7925426E-421.1149656
AGTACTT107950.020.99216812-13
GTACTTT107750.020.30125814-15
CATGGGG35350.020.1545354
GCAGAGT195650.019.9617619
Produced by FastQC (version 0.11.5)