##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937048_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1917432 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.115179573512904 31.0 31.0 34.0 31.0 34.0 2 32.247545154143666 33.0 31.0 34.0 30.0 34.0 3 32.369371638733476 34.0 31.0 34.0 31.0 34.0 4 36.047320582946355 37.0 35.0 37.0 35.0 37.0 5 35.89279411212497 37.0 35.0 37.0 35.0 37.0 6 35.98570223090049 37.0 35.0 37.0 35.0 37.0 7 35.93988939373078 37.0 35.0 37.0 35.0 37.0 8 35.88587913417529 37.0 35.0 37.0 35.0 37.0 9 37.58210669270149 39.0 37.0 39.0 35.0 39.0 10-11 37.49083226941033 39.0 37.0 39.0 35.0 39.0 12-13 37.38779680322432 39.0 37.0 39.0 34.0 39.0 14-15 38.56157845493347 40.0 38.0 41.0 34.0 41.0 16-17 38.56395872187384 40.0 38.0 41.0 34.0 41.0 18-19 38.712972872049704 40.0 38.0 41.0 34.0 41.0 20-21 38.72340818344536 40.0 38.0 41.0 34.0 41.0 22-23 38.62611555455422 40.0 38.0 41.0 34.0 41.0 24-25 38.53947571543606 40.0 38.0 41.0 34.0 41.0 26-27 38.33522596890007 40.0 38.0 41.0 34.0 41.0 28-29 38.1830659444507 40.0 38.0 41.0 34.0 41.0 30-31 38.14832651170941 40.0 37.5 41.0 34.0 41.0 32-33 37.99877570625712 40.0 37.0 41.0 33.5 41.0 34-35 37.858883652718845 40.0 37.0 41.0 33.0 41.0 36-37 37.695025951376635 40.0 37.0 41.0 33.0 41.0 38-39 37.522933538190664 40.0 37.0 41.0 33.0 41.0 40-41 37.19055356330759 40.0 36.0 41.0 32.0 41.0 42-43 36.97054784732914 39.0 36.0 41.0 31.0 41.0 44-45 36.70122669278493 39.0 36.0 41.0 31.0 41.0 46-47 36.272423480989154 39.0 35.5 41.0 30.0 41.0 48-49 35.854236291039264 39.0 35.0 40.0 29.0 41.0 50-51 35.55361363532057 39.0 35.0 40.0 28.0 41.0 52-53 35.30965374521756 38.5 35.0 40.0 27.0 41.0 54-55 34.94663956792209 38.0 34.5 40.0 26.0 41.0 56-57 34.74065886039244 38.0 34.0 40.0 26.0 41.0 58-59 34.42862093675291 38.0 34.0 40.0 25.5 41.0 60-61 34.056861468881294 37.5 33.5 40.0 24.5 41.0 62-63 33.689425231246794 37.0 33.0 39.5 23.0 41.0 64-65 33.36639891271242 36.5 33.0 39.0 23.0 40.0 66-67 33.039182093550124 36.0 32.0 39.0 22.0 40.0 68-69 32.58971947896978 35.5 32.0 39.0 20.5 40.0 70-71 32.14004199366653 35.0 31.0 38.0 20.0 40.0 72-73 31.716968580893614 35.0 31.0 37.5 18.0 39.5 74-75 31.232678916383996 35.0 31.0 37.0 17.5 39.0 76-77 29.821284144626773 33.5 28.5 35.5 13.0 38.0 78-79 30.47768577973039 34.0 30.0 36.0 13.0 38.0 80-81 30.32756598408705 34.0 30.0 36.0 9.5 37.5 82-83 29.96248654450327 34.0 30.0 35.0 7.0 37.0 84-85 29.499420839956777 34.0 29.0 35.0 4.5 36.5 86-87 29.123741545984423 34.0 29.0 35.0 2.0 36.0 88-89 28.727204145961892 34.0 29.0 35.0 2.0 36.0 90-91 28.259706211224177 33.0 29.0 35.0 2.0 35.0 92-93 27.76611217503411 33.0 27.0 35.0 2.0 35.0 94-95 27.191855304386284 33.0 26.5 34.0 2.0 35.0 96-97 26.41404467016301 32.0 25.0 34.0 2.0 35.0 98-99 25.53079118320754 32.0 23.5 34.0 2.0 35.0 100-101 22.313532631144156 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14885839608410123 1101 2 0.23943666650906437 1101 3 0.16303874352723824 1101 4 0.08845903894027884 1101 5 0.04054534316436076 1101 6 0.0767895800216607 1101 7 0.051462630286074784 1101 8 0.21361126117108853 1101 9 0.24204310720387667 1101 10-11 0.2483075635484866 1101 12-13 0.21050016321661502 1101 14-15 0.26884676093300186 1101 16-17 0.3544438217828443 1101 18-19 0.111044676708147 1101 20-21 0.0461824363396417 1101 22-23 0.17156230138761686 1101 24-25 0.10157806268395575 1101 26-27 0.0248548002777369 1101 28-29 0.1532658924345256 1101 30-31 0.2133403524732529 1101 32-33 0.3441067455265099 1101 34-35 0.17313686733984923 1101 36-37 0.2147532819615421 1101 38-39 0.1394700663398325 1101 40-41 0.37854562840695394 1101 42-43 0.29893580505021333 1101 44-45 0.21705206044553194 1101 46-47 0.11112163446831147 1101 48-49 0.13744187163171517 1101 50-51 0.20453037073515645 1101 52-53 0.3146270694701343 1101 54-55 0.06365800326918247 1101 56-57 0.15115226997533426 1101 58-59 0.31895728621390873 1101 60-61 0.3150495201642798 1101 62-63 0.1863189009447126 1101 64-65 0.184007287814552 1101 66-67 0.16118558439021058 1101 68-69 -0.22569336703330833 1101 70-71 -0.18826165964191333 1101 72-73 -0.045873994167550336 1101 74-75 -0.04318124479258145 1101 76-77 -0.134760794547784 1101 78-79 0.11003510981858611 1101 80-81 -0.004343634724314427 1101 82-83 0.19630000157633276 1101 84-85 0.09778371358842719 1101 86-87 -0.06116644819464767 1101 88-89 -0.08463364292258646 1101 90-91 -0.030743106948229126 1101 92-93 -0.0015542208357750553 1101 94-95 -0.2560912772879682 1101 96-97 -0.363964398966921 1101 98-99 0.08602293401409966 1101 100-101 0.7499516570754565 1102 1 -0.15777280831886742 1102 2 -0.05145690324628305 1102 3 -0.017681515753778854 1102 4 -0.022854970887152604 1102 5 -0.13149839916742678 1102 6 0.03607826421136906 1102 7 0.0956524818532003 1102 8 0.06324530771419035 1102 9 0.14978201395027924 1102 10-11 0.12127946363437303 1102 12-13 0.19673577052172675 1102 14-15 0.149953826188316 1102 16-17 0.14272636897887025 1102 18-19 0.104540924553433 1102 20-21 0.03553233119413335 1102 22-23 -0.07889305215214648 1102 24-25 0.016395658988571427 1102 26-27 0.060751599619734975 1102 28-29 -0.1251994384591839 1102 30-31 -0.04109775398313076 1102 32-33 -0.06308752012643026 1102 34-35 0.044773738884018144 1102 36-37 -0.04684626625988386 1102 38-39 0.17237159935043422 1102 40-41 0.19266703281923725 1102 42-43 0.15493631818314668 1102 44-45 0.23287530380920884 1102 46-47 0.125017599709615 1102 48-49 0.20202806472031654 1102 50-51 0.025787335207631656 1102 52-53 0.05179324957806131 1102 54-55 0.18524488590180255 1102 56-57 0.07207301465518157 1102 58-59 -0.13357047342936568 1102 60-61 0.056848958245630854 1102 62-63 0.025805587967042243 1102 64-65 0.12608031023156485 1102 66-67 0.08440469370985682 1102 68-69 0.042991709056309446 1102 70-71 -0.025795706202323743 1102 72-73 -0.20766373396725513 1102 74-75 -0.11968306317710997 1102 76-77 0.07006208370208 1102 78-79 -0.3220720712551852 1102 80-81 0.05671961687303906 1102 82-83 0.042664643462696716 1102 84-85 -0.04271421232618877 1102 86-87 -0.17325310048874698 1102 88-89 -0.1815247913968392 1102 90-91 -0.2938954709000541 1102 92-93 -0.24669871778805685 1102 94-95 -0.14116127301705106 1102 96-97 0.15759281059403918 1102 98-99 0.019495266817852297 1102 100-101 -0.025375928268818626 1103 1 0.07319528219715465 1103 2 0.14201593964278203 1103 3 0.14781358109183174 1103 4 0.12050306304588787 1103 5 -0.005549138234016482 1103 6 0.026246825195663348 1103 7 0.017781910508013254 1103 8 0.18230755432964685 1103 9 0.1574404500240334 1103 10-11 0.05375416825427948 1103 12-13 0.22765936911664397 1103 14-15 0.13863028006226585 1103 16-17 0.20363089715984728 1103 18-19 0.1681219168889072 1103 20-21 0.11684840869346402 1103 22-23 0.1737479536356048 1103 24-25 0.14565392326296944 1103 26-27 0.21786386261743473 1103 28-29 0.09853762018885703 1103 30-31 0.12628477506559932 1103 32-33 0.027020138138304617 1103 34-35 0.09800421979085172 1103 36-37 0.126441960857953 1103 38-39 0.1952897934239317 1103 40-41 0.32641465948842097 1103 42-43 0.11604196431645164 1103 44-45 0.19670757935679717 1103 46-47 0.12026094310328261 1103 48-49 0.15898627182344427 1103 50-51 0.12697152090933628 1103 52-53 0.021661729022397935 1103 54-55 0.15207079950446456 1103 56-57 0.058816535388281466 1103 58-59 -0.026431947442709713 1103 60-61 -0.2817711144454478 1103 62-63 0.09180193761147848 1103 64-65 0.06714279329960249 1103 66-67 0.12469939128949648 1103 68-69 0.16098554034758905 1103 70-71 0.144055772264025 1103 72-73 0.20605611238045896 1103 74-75 -0.03833795265266815 1103 76-77 0.10812342772362982 1103 78-79 0.07235899472897955 1103 80-81 -0.036749589748623634 1103 82-83 0.16945253046577946 1103 84-85 -0.0716623277401709 1103 86-87 -0.45412991307761885 1103 88-89 -0.09022592239023197 1103 90-91 -0.19187323027015069 1103 92-93 -0.2349347263924919 1103 94-95 0.023588960151457172 1103 96-97 0.27545508896496784 1103 98-99 0.018563735067811393 1103 100-101 0.6322999561770786 1104 1 -0.003827740710114824 1104 2 0.01372544090948935 1104 3 -0.007277316699124015 1104 4 -0.047126491561328976 1104 5 -0.15106096303497196 1104 6 0.08958239548670122 1104 7 0.073177038877553 1104 8 -0.002184720153941555 1104 9 -0.02958697100627461 1104 10-11 0.13529658199011152 1104 12-13 0.1695788087554959 1104 14-15 0.1254301723937843 1104 16-17 0.062262533025574385 1104 18-19 0.13511206539646992 1104 20-21 0.2162509699125792 1104 22-23 0.18435098156249552 1104 24-25 0.05240916739127499 1104 26-27 0.017978698887510802 1104 28-29 0.07209398665060718 1104 30-31 0.12456482961012227 1104 32-33 0.048858843743197156 1104 34-35 0.16915842283515303 1104 36-37 0.11026158887332116 1104 38-39 0.028852236268356535 1104 40-41 0.17428356207335582 1104 42-43 0.0980528615270515 1104 44-45 0.17869931192493027 1104 46-47 0.2036200999802773 1104 48-49 0.22116200228325766 1104 50-51 0.36862163777034596 1104 52-53 0.20666527718812233 1104 54-55 0.38163582275431196 1104 56-57 0.33393179934387973 1104 58-59 0.1519695438046753 1104 60-61 0.16698663776070077 1104 62-63 0.24492039307555302 1104 64-65 0.17408052380314842 1104 66-67 -0.12656769474719454 1104 68-69 0.0031072156881535307 1104 70-71 -0.023587316695206084 1104 72-73 0.0036549667462821844 1104 74-75 -0.16534214656939739 1104 76-77 0.029903210173532813 1104 78-79 0.18218972004681788 1104 80-81 0.1975589531993407 1104 82-83 0.19425894050813852 1104 84-85 -0.04114431286797782 1104 86-87 0.024494905805685363 1104 88-89 0.022725283130746732 1104 90-91 1.1176976990867615E-4 1104 92-93 -0.014619079317288453 1104 94-95 -0.2880747928399039 1104 96-97 0.045607143620365775 1104 98-99 -0.029314739010875 1104 100-101 0.7832283369336999 1105 1 0.09164666352467066 1105 2 0.13952059681375317 1105 3 0.06556711957661321 1105 4 0.05561700019451621 1105 5 0.055833530965401224 1105 6 0.16446131696289257 1105 7 0.12789251727596707 1105 8 0.14117050159858024 1105 9 0.21617358989740154 1105 10-11 0.17360569166214646 1105 12-13 0.15869546663018497 1105 14-15 0.20538933947219817 1105 16-17 0.09907302991894795 1105 18-19 0.07892621990755089 1105 20-21 0.1714274497678403 1105 22-23 0.12353284321591218 1105 24-25 0.07117981956432118 1105 26-27 0.09975593349938094 1105 28-29 0.020189550963657155 1105 30-31 0.08155579655947776 1105 32-33 0.07978349618198166 1105 34-35 0.09377267027851843 1105 36-37 -0.10647354375013407 1105 38-39 -0.08723634857492613 1105 40-41 -0.048554628324865234 1105 42-43 -0.13015121396770724 1105 44-45 -0.25129951846487586 1105 46-47 -0.3943777183386814 1105 48-49 -0.527429492661561 1105 50-51 -0.5753882474248542 1105 52-53 -0.4325028196651459 1105 54-55 -0.3598532740122309 1105 56-57 -0.5991040392626488 1105 58-59 -0.5073846722422175 1105 60-61 -0.48338544945243456 1105 62-63 -0.47522093084360506 1105 64-65 -0.3325138498148945 1105 66-67 -0.3993856357717789 1105 68-69 -0.4407005735974252 1105 70-71 -0.49071371177362266 1105 72-73 -0.5525611135637689 1105 74-75 -0.6095505472348819 1105 76-77 -0.4050449808691603 1105 78-79 -0.2329996175639515 1105 80-81 -0.24676959109745766 1105 82-83 -0.3062439123063605 1105 84-85 -0.6879268107256813 1105 86-87 -0.4980877207887069 1105 88-89 -0.3066449016756181 1105 90-91 -0.440837237612957 1105 92-93 -0.43619910333010026 1105 94-95 -0.1542053483888317 1105 96-97 -0.07565079998214586 1105 98-99 -0.029614302435970075 1105 100-101 0.1753401516674451 1106 1 0.066388557647862 1106 2 0.13714535487565627 1106 3 0.07451130734239797 1106 4 0.05459899213112607 1106 5 0.0029070305524498963 1106 6 0.09833509718821176 1106 7 0.09017199570140377 1106 8 0.19228250761339183 1106 9 0.1532706765891021 1106 10-11 0.1376254381751636 1106 12-13 0.2773525886860213 1106 14-15 0.3009766266377554 1106 16-17 0.18356720323622255 1106 18-19 0.07901251419288258 1106 20-21 0.0212195169094187 1106 22-23 0.17461343943337937 1106 24-25 0.07619315384987146 1106 26-27 0.1938054631128665 1106 28-29 0.0958622000395124 1106 30-31 0.1522141194614548 1106 32-33 0.0198033471739123 1106 34-35 -0.06850042699861092 1106 36-37 0.006192076804971691 1106 38-39 2.9862375470202096E-4 1106 40-41 0.22470631038503086 1106 42-43 0.05064622457985024 1106 44-45 -0.21175987367993088 1106 46-47 -0.17057629967384713 1106 48-49 -0.1603003745891627 1106 50-51 -0.027717597995888354 1106 52-53 -0.05744140524561203 1106 54-55 0.07304217368318433 1106 56-57 -0.1523127319075357 1106 58-59 -0.1433732245282897 1106 60-61 -0.19719160934351265 1106 62-63 -0.2132865303706808 1106 64-65 -0.16166819226896934 1106 66-67 -0.19289961180908222 1106 68-69 -0.1788437057295127 1106 70-71 -0.2637405648359419 1106 72-73 -0.27120504350471464 1106 74-75 -0.17227673910443997 1106 76-77 -0.10888282828631546 1106 78-79 -0.11061944263452261 1106 80-81 -0.007956934337769894 1106 82-83 0.14651310745271573 1106 84-85 0.18340411165773673 1106 86-87 0.17811529098104018 1106 88-89 0.25113871060977644 1106 90-91 0.22450426623523967 1106 92-93 0.22064406655987412 1106 94-95 0.49612557862339557 1106 96-97 0.49181992443358524 1106 98-99 0.3342400962912393 1106 100-101 0.6796875982492985 1107 1 0.22302166821934577 1107 2 0.3159763787729233 1107 3 0.2403750713413757 1107 4 0.1578265299471866 1107 5 0.08253430262517725 1107 6 0.2143490781672739 1107 7 0.23141113851553285 1107 8 0.20049780552737673 1107 9 0.2601153672861187 1107 10-11 0.2280902599111343 1107 12-13 0.3178416284100649 1107 14-15 0.3825077925702516 1107 16-17 0.39884179922128027 1107 18-19 0.3375407641486987 1107 20-21 0.3932736768397831 1107 22-23 0.21423807698173647 1107 24-25 0.4146628551831668 1107 26-27 0.3634446969080898 1107 28-29 0.3964045684278332 1107 30-31 0.4088826570222466 1107 32-33 0.22828954292071302 1107 34-35 0.2248064083648842 1107 36-37 0.31222808306237226 1107 38-39 0.32848795278552245 1107 40-41 0.5274667519378653 1107 42-43 0.39602664335468774 1107 44-45 0.4270247433158616 1107 46-47 0.27510954393030573 1107 48-49 0.2814975001187534 1107 50-51 0.10276213089355224 1107 52-53 0.21461414558271485 1107 54-55 0.35136331075045035 1107 56-57 0.2904747257928051 1107 58-59 0.16391581715937775 1107 60-61 -0.042817480700179544 1107 62-63 0.12497494535570297 1107 64-65 0.26795973434398235 1107 66-67 0.3257318755026546 1107 68-69 0.09224230740056072 1107 70-71 -0.0055649484226947266 1107 72-73 0.25784795134405414 1107 74-75 0.3065280213665673 1107 76-77 -0.14387454835255298 1107 78-79 0.21189665905484745 1107 80-81 0.1725546215697804 1107 82-83 0.3789681569032268 1107 84-85 0.33727997155994416 1107 86-87 0.4665678924511383 1107 88-89 0.558730477148373 1107 90-91 0.6495070480311682 1107 92-93 0.6673951743691937 1107 94-95 0.7278733155505925 1107 96-97 0.892513900699953 1107 98-99 1.161877975188208 1107 100-101 1.5869764763019099 1108 1 0.03724997546164133 1108 2 0.10061920249641076 1108 3 0.02308706027882579 1108 4 0.03117381854741552 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-1.251158172073609 2316 90-91 -1.3442594247965118 2316 92-93 -1.3180624410913317 2316 94-95 -1.5891098775509391 2316 96-97 -1.4127255966803887 2316 98-99 -1.9911176800458144 2316 100-101 -2.7721869993632176 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 6.0 4 15.0 5 47.0 6 88.0 7 217.0 8 516.0 9 1062.0 10 1869.0 11 3064.0 12 4885.0 13 7008.0 14 8957.0 15 12527.0 16 16953.0 17 23901.0 18 21231.0 19 12435.0 20 10201.0 21 10448.0 22 11127.0 23 12080.0 24 13719.0 25 16358.0 26 20103.0 27 24568.0 28 30358.0 29 38536.0 30 48247.0 31 60873.0 32 76539.0 33 100110.0 34 135730.0 35 192305.0 36 292982.0 37 424857.0 38 269954.0 39 13553.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63863350052034 20.34944953482367 15.447296969507281 21.564619995148707 2 15.266164732781748 21.51885068405703 40.54133131391521 22.673653269246014 3 18.952762168011787 26.33130370157894 30.663624392088344 24.052309738320925 4 12.703814268250452 18.047471826901816 38.38399484310265 30.864719061745085 5 12.922898312474834 38.31859782493048 34.6191437828185 14.139360079776193 6 30.004975404603655 38.1227078717785 17.70169685287405 14.170619870743787 7 27.08892025037733 33.928036857745745 21.668528537737572 17.31451435413935 8 25.79856808481344 35.27181146450044 20.17552643327117 18.75409401741496 9 26.41105395132657 17.149447803103318 21.255564734499057 35.18393351107106 10-11 24.158744612586 27.739732100017108 27.779055528435947 20.322467758960943 12-13 25.535786747225487 25.26704581609097 28.48385259488211 20.713314841801434 14-15 22.479947803528894 26.330767493614953 27.411935405302362 23.777349297553783 16-17 20.855759161211456 29.727494899427988 27.968605927094153 21.448140012266407 18-19 20.7163936396941 27.801030914662306 31.153498431619305 20.32907701402429 20-21 21.66500165458131 26.764110944827213 31.47271818373128 20.098169216860196 22-23 21.385132871024346 26.724094994040122 31.328209566630306 20.562562568305225 24-25 21.24932396171733 26.95506099077876 31.444737488532777 20.35087755897114 26-27 21.035762760260816 27.088012454186668 31.486379537166332 20.389845248386184 28-29 20.726164141978476 27.088660012104736 31.680427697156325 20.504748148760466 30-31 20.929477551224764 26.892374801296736 31.6146804684599 20.563467179018605 32-33 20.909051083013285 27.13749877439987 31.457921757687902 20.495528384898936 34-35 21.264613295282437 26.98914485624523 31.297641845968982 20.448600002503348 36-37 21.269399617451214 26.753329048022 31.264692728834977 20.712578605691814 38-39 20.871505262073562 27.33485199562396 31.247445896025965 20.54619684627652 40-41 21.142067985750618 27.18011449259981 31.19335313949786 20.484464382151714 42-43 20.997015800299568 27.320030123623678 30.78969684452956 20.893257231547192 44-45 21.180883418644015 27.28027772975276 30.576897351027053 20.96194150057617 46-47 21.56772397118424 27.545643293107467 29.967305075614508 20.91932766009378 48-49 21.580428380808787 27.915237561537467 29.596785696684023 20.907548360969724 50-51 21.601993332090323 28.044779563912968 29.400292324080752 20.952934779915957 52-53 21.618185848207883 28.255107761711674 29.130187621419353 20.996518768661097 54-55 21.57826196704759 28.12459581356731 29.107837983302666 21.189304236082425 56-57 21.59056861341563 28.408270977651046 28.90516069056066 21.095999718372664 58-59 21.791700816118663 27.957716307170617 29.128163512661093 21.12241936404963 60-61 21.645884558446372 28.143921347432677 29.40307797899702 20.807116115123936 62-63 21.474170002944042 28.16001510353825 29.518525172867978 20.847289720649734 64-65 21.535893198792238 28.122647406178526 29.30297640358991 21.038482991439327 66-67 21.569056946999947 28.280064169159587 28.898964865507615 21.251914018332855 68-69 21.465590817716304 28.184083759967436 29.210039576381213 21.140285845935043 70-71 21.697171988474125 28.14269136993624 29.095375308030302 21.064761333559332 72-73 21.652245430986422 28.06617931217199 29.061523593857814 21.22005166298378 74-75 21.557628418093536 28.618237481550246 28.695478702597832 21.12865539775839 76-77 21.590961243997178 28.311356022012774 28.83027924849486 21.267403485495183 78-79 21.569843336930166 28.232365560722783 28.652694470245805 21.54509663210125 80-81 21.5735728875826 28.597375240202265 28.438748520453146 21.39030335176199 82-83 21.73736629896982 28.47296721498714 28.540896647649905 21.24876983839314 84-85 21.518561854899886 28.48739677681052 28.38992034209065 21.604121026198943 86-87 21.32861416069925 28.539529416121034 28.58735393507913 21.544502488100587 88-89 21.6062910650229 28.42737456099085 28.39073613763739 21.57559823634886 90-91 21.497420080113507 28.277731694068724 28.53260749101194 21.692240734805825 92-93 21.51127132539772 28.293206747357925 28.552511901334704 21.643010025909653 94-95 21.40704337885255 28.0469920184914 28.631784595229455 21.9141800074266 96-97 21.46364258171817 28.14042348906361 28.591965749961474 21.80396817925675 98-99 21.240009043395 28.301993067252035 28.38825474732603 22.069743142026933 100-101 20.877776331946986 28.761642451029147 28.284613906243926 22.075967310779937 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 670.0 1 508.0 2 379.0 3 447.5 4 413.0 5 398.0 6 672.5 7 1418.5 8 2424.0 9 2943.5 10 2816.0 11 2610.0 12 2684.0 13 2949.5 14 3252.0 15 3579.5 16 3877.0 17 4073.0 18 4333.0 19 4622.0 20 5023.0 21 5643.5 22 6463.0 23 7649.5 24 9050.5 25 11042.0 26 14011.5 27 17253.0 28 19803.0 29 23101.5 30 27582.5 31 31870.5 32 36809.0 33 42406.0 34 48470.5 35 53720.0 36 60666.0 37 66998.5 38 68171.5 39 67788.0 40 66957.0 41 66292.5 42 65357.5 43 66931.0 44 69836.5 45 70554.0 46 68963.0 47 68651.0 48 72943.5 49 71058.5 50 64103.0 51 59326.0 52 55417.5 53 52808.5 54 49597.0 55 45251.0 56 40151.5 57 34756.0 58 31042.0 59 28187.0 60 24127.5 61 19379.0 62 14991.5 63 11421.0 64 8428.5 65 6222.5 66 4684.5 67 3681.5 68 2712.5 69 1646.5 70 1107.0 71 783.5 72 547.0 73 388.5 74 278.0 75 227.0 76 161.5 77 87.5 78 48.0 79 30.0 80 20.0 81 7.5 82 3.0 83 1.5 84 0.0 85 0.0 86 2.0 87 2.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021747837732967845 2 0.0025033482282552917 3 3.6507161662056333E-4 4 0.0 5 0.0035464099900283296 6 0.0 7 3.1291852853191146E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.008240187918007002 14-15 0.003181338373407766 16-17 0.0 18-19 4.433012487535412E-4 20-21 0.0014863630105265793 22-23 0.0014342099224379273 24-25 5.73683968975171E-4 26-27 2.3468889639893356E-4 28-29 2.6076544044325953E-5 30-31 0.0 32-33 2.0861235235460762E-4 34-35 0.0 36-37 0.0015385160986152312 38-39 0.009100713871469757 40-41 8.083728653741045E-4 42-43 0.0 44-45 0.0014863630105265793 46-47 4.693777927978671E-4 48-49 1.3038272022162977E-4 50-51 0.0020600469795017504 52-53 3.6507161662056333E-4 54-55 0.0 56-57 2.3468889639893356E-4 58-59 1.3038272022162977E-4 60-61 0.001955740803324446 62-63 2.6076544044325953E-5 64-65 2.6076544044325953E-5 66-67 0.0 68-69 2.6076544044325953E-5 70-71 3.6507161662056333E-4 72-73 2.6076544044325954E-4 74-75 0.003233491461496418 76-77 0.0 78-79 2.6076544044325954E-4 80-81 0.001955740803324446 82-83 5.2153088088651906E-5 84-85 0.002372965508033662 86-87 2.868419844875855E-4 88-89 0.0023990420520779874 90-91 0.0014342099224379273 92-93 0.0 94-95 0.0 96-97 1.8253580831028166E-4 98-99 5.476074249308449E-4 100-101 0.0021643531556790542 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1917432.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.29370020866916 #Duplication Level Percentage of deduplicated Percentage of total 1 77.43828495473484 42.04411028005869 2 12.954641055249844 14.067107955293052 3 4.025678843278074 6.557070007599654 4 1.855332630766018 4.029314945686866 5 0.9653735008127202 2.6206849721259635 6 0.5833744601970925 1.9004134830801087 7 0.3816457080366609 1.4504670360647423 8 0.26340185585241177 1.1440849116846348 9 0.2092414977996779 1.0224445637473758 >10 1.1488951539735957 11.719405324178132 >50 0.10733483801781657 4.037154891748722 >100 0.06303711137034508 6.328418437262442 >500 0.002891069162235039 1.0796702641492308 >1k 5.782138324470076E-4 0.5742370316955565 >5k 1.9273794414900259E-4 0.811099996842325 >10k+ 9.636897207450129E-5 0.6143158987824627 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11741 0.6123294072488621 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9293 0.48465864760784216 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6209 0.32381852394243965 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3243 0.16913246467149815 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2516 0.13121716963104818 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0430617617730381E-4 0.0 10-11 0.0 0.0 0.0 1.3038272022162974E-4 5.2153088088651906E-5 12-13 0.0 0.0 0.0 1.564592642659557E-4 5.2153088088651906E-5 14-15 0.0 0.0 0.0 1.564592642659557E-4 5.2153088088651906E-5 16-17 0.0 0.0 0.0 1.564592642659557E-4 5.2153088088651906E-5 18-19 0.0 0.0 0.0 1.8253580831028166E-4 5.2153088088651906E-5 20-21 0.0 0.0 0.0 2.8684198448758545E-4 5.2153088088651906E-5 22-23 0.0 0.0 0.0 5.476074249308449E-4 5.2153088088651906E-5 24-25 0.0 0.0 0.0 0.0010691383058173641 5.2153088088651906E-5 26-27 0.0 0.0 0.0 0.0015385160986152312 5.2153088088651906E-5 28-29 0.0 0.0 0.0 0.002711960580609899 5.2153088088651906E-5 30-31 0.0 0.0 0.0 0.005449997705264125 5.2153088088651906E-5 32-33 0.0 0.0 0.0 0.008813871886982173 5.2153088088651906E-5 34-35 0.0 0.0 0.0 0.013064348566207303 5.2153088088651906E-5 36-37 0.0 0.0 0.0 0.016923677084767545 5.2153088088651906E-5 38-39 0.0 0.0 0.0 0.021122000675904022 5.2153088088651906E-5 40-41 0.0 0.0 0.0 0.027745442863162814 5.2153088088651906E-5 42-43 0.0 0.0 0.0 0.037524146879785045 5.2153088088651906E-5 44-45 0.0 0.0 0.0 0.04675524347147643 5.2153088088651906E-5 46-47 0.0 0.0 0.0 0.05734232035347277 5.2153088088651906E-5 48-49 0.0 0.0 0.0 0.067121024370095 5.2153088088651906E-5 50-51 0.0 0.0 0.0 0.08073298036123315 5.2153088088651906E-5 52-53 0.0 0.0 0.0 0.11087746527647395 5.2153088088651906E-5 54-55 0.0 0.0 0.0 0.1490274492133228 5.2153088088651906E-5 56-57 0.0 0.0 0.0 0.1856649935956008 5.2153088088651906E-5 58-59 0.0 0.0 0.0 0.24712740790807705 5.2153088088651906E-5 60-61 0.0 0.0 0.0 0.32259292637235637 5.2153088088651906E-5 62-63 0.0 0.0 0.0 0.3689830982272122 5.2153088088651906E-5 64-65 0.0 0.0 0.0 0.40384743761447606 5.2153088088651906E-5 66-67 0.0 0.0 0.0 0.5059631840920564 5.2153088088651906E-5 68-69 0.0 0.0 0.0 0.6176750987779489 5.2153088088651906E-5 70-71 0.0 0.0 0.0 0.6660210114361291 5.2153088088651906E-5 72-73 0.0 0.0 0.0 0.7621130762394703 5.2153088088651906E-5 74-75 0.0 0.0 0.0 0.9062642117165042 5.2153088088651906E-5 76-77 0.0 0.0 0.0 1.0468428606594653 5.2153088088651906E-5 78-79 0.0 0.0 0.0 1.1848138551979939 5.2153088088651906E-5 80-81 0.0 0.0 0.0 1.3484441690761395 5.2153088088651906E-5 82-83 0.0 0.0 0.0 1.4917608551437547 5.2153088088651906E-5 84-85 0.0 0.0 0.0 1.6509320799903204 5.2153088088651906E-5 86-87 0.0 0.0 0.0 1.9380087533743047 5.2153088088651906E-5 88-89 0.0 0.0 0.0 2.338648776071329 5.2153088088651906E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8585 0.0 32.318916 1 GTATCAA 12830 0.0 31.32766 1 TCAACGC 16015 0.0 24.942501 4 ATCAACG 16015 0.0 24.912844 3 TATCAAC 16150 0.0 24.67647 2 CAACGCA 16245 0.0 24.618597 5 AACGCAG 16595 0.0 24.133661 6 GTACATG 9115 0.0 24.132849 1 CGTATAG 205 2.665729E-8 23.175566 1 TACATGG 9325 0.0 23.024128 2 ACATGGG 9035 0.0 22.710491 3 GAGTACT 10155 0.0 21.683601 12-13 ACGCAGA 18545 0.0 21.467926 7 CGCAGAG 18510 0.0 21.330523 8 AGAGTAC 15000 0.0 21.301388 10-11 GTACCGT 135 3.7925426E-4 21.114965 6 AGTACTT 10795 0.0 20.992168 12-13 GTACTTT 10775 0.0 20.301258 14-15 CATGGGG 3535 0.0 20.154535 4 GCAGAGT 19565 0.0 19.961761 9 >>END_MODULE