FastQCFastQC Report
Thu 26 May 2016
SRR937047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563472
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129620.8290522631681283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91160.5830612892331938No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60340.38593591698476215No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30340.19405528209011738No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21260.13597940992867158No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19560.12510617395130838No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17460.11167452950868323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA135100.034.5512121
GGTATCA86700.033.4439771
TATCAAC167850.027.8097672
ATCAACG168300.027.5350553
TCAACGC168550.027.5224114
CAACGCA169550.027.3854875
AACGCAG172550.026.93696
GTACATG105050.025.8825531
GAGTACT89400.025.11414712-13
TACATGG108750.024.7396972
ACGCAGA190650.024.0796477
CGCAGAG190950.023.8434758
ACATGGG104650.023.6172623
GTACTTT95300.023.60995514-15
AGTACTT94900.023.45835312-13
GCAGAGT195300.022.6811569
AGAGTAC148950.022.65092310-11
CATGGGA67300.021.8933434
GTATAGG5550.021.411851
TACTTTT105250.020.8361614-15
Produced by FastQC (version 0.11.5)