##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937047_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1563472 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.863772424450197 33.0 31.0 34.0 30.0 34.0 2 31.955724822702294 33.0 31.0 34.0 30.0 34.0 3 31.950789013170688 33.0 31.0 34.0 30.0 34.0 4 35.549927980801705 37.0 35.0 37.0 33.0 37.0 5 35.583154671142175 37.0 35.0 37.0 33.0 37.0 6 35.65603797189844 37.0 35.0 37.0 33.0 37.0 7 35.59991480499811 37.0 35.0 37.0 33.0 37.0 8 35.572408076383844 37.0 35.0 37.0 33.0 37.0 9 37.19284451528393 39.0 37.0 39.0 34.0 39.0 10-11 37.07394184225877 39.0 37.0 39.0 33.0 39.0 12-13 36.96842380292068 39.0 37.0 39.0 33.0 39.0 14-15 38.09295593397259 40.0 38.0 41.0 33.0 41.0 16-17 38.03889004727939 40.0 38.0 41.0 33.0 41.0 18-19 38.048250944052725 40.0 38.0 41.0 33.0 41.0 20-21 38.016741265593495 40.0 38.0 41.0 33.0 41.0 22-23 37.87287172395796 40.0 38.0 41.0 33.0 41.0 24-25 37.70171419763194 40.0 37.0 41.0 32.0 41.0 26-27 37.65307117748191 40.0 37.0 41.0 32.5 41.0 28-29 37.71222509901041 40.0 37.0 41.0 33.0 41.0 30-31 37.54270335509686 40.0 37.0 41.0 32.0 41.0 32-33 37.30859075186508 40.0 36.0 41.0 32.0 41.0 34-35 37.09711430713182 39.5 36.0 41.0 31.0 41.0 36-37 36.92997092368779 39.0 36.0 41.0 31.0 41.0 38-39 36.755675509379124 39.0 35.0 41.0 31.0 41.0 40-41 36.533375717633575 39.0 35.0 41.0 30.0 41.0 42-43 36.27721187203864 39.0 35.0 41.0 30.0 41.0 44-45 35.94473677814506 39.0 35.0 40.5 29.5 41.0 46-47 35.47350288332635 39.0 35.0 40.0 27.5 41.0 48-49 35.04122811281558 38.0 35.0 40.0 25.5 41.0 50-51 34.34223062517269 37.5 33.5 39.5 24.0 40.5 52-53 34.61766504293009 38.0 34.5 40.0 24.5 40.5 54-55 34.9151900385808 39.0 35.0 40.0 25.5 41.0 56-57 34.7842820338324 38.5 35.0 40.5 24.5 41.0 58-59 34.53645764043104 38.0 34.0 40.0 24.0 41.0 60-61 34.24062151416847 38.0 34.0 40.0 23.0 41.0 62-63 33.94165741375605 37.5 34.0 40.0 22.5 41.0 64-65 33.62664537644422 37.0 33.5 40.0 22.0 41.0 66-67 33.24756471494213 36.5 33.0 39.0 20.5 41.0 68-69 32.807080331467404 36.0 33.0 39.0 19.0 41.0 70-71 32.4502172088787 35.0 32.5 39.0 18.5 40.5 72-73 32.07864611582427 35.0 32.0 38.0 17.5 40.0 74-75 31.661366816930524 35.0 32.0 37.0 13.5 39.5 76-77 31.242572620424284 35.0 31.0 37.0 9.5 39.0 78-79 30.829190097424195 35.0 31.0 36.0 7.0 39.0 80-81 30.420586041835094 35.0 31.0 36.0 4.5 37.5 82-83 30.02838937953478 34.5 30.5 35.0 2.0 37.0 84-85 29.685101811864875 34.0 30.0 35.0 2.0 37.0 86-87 29.39209496556382 34.0 30.0 35.0 2.0 36.0 88-89 29.03446336103237 34.0 29.0 35.0 2.0 36.0 90-91 28.708861431480706 34.0 29.0 35.0 2.0 35.5 92-93 28.369384293418747 34.0 29.0 35.0 2.0 35.0 94-95 27.99758550201091 34.0 29.0 35.0 2.0 35.0 96-97 27.594375211068698 34.0 28.0 35.0 2.0 35.0 98-99 27.03906689726455 34.0 27.0 35.0 2.0 35.0 100-101 24.920811821382152 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3577411748462147 1101 2 0.3192395708935223 1101 3 0.4601199223546324 1101 4 0.34035169297894896 1101 5 0.24415727666582399 1101 6 0.1576387394415164 1101 7 0.2242378387213222 1101 8 0.16690846303249884 1101 9 0.2750810910901862 1101 10-11 0.19195657322345738 1101 12-13 0.35792548040289773 1101 14-15 0.6915477271225541 1101 16-17 0.6524586795426757 1101 18-19 0.6013691227942388 1101 20-21 0.5211067090869861 1101 22-23 0.5514441509828103 1101 24-25 0.7654388300786579 1101 26-27 0.7746881846162594 1101 28-29 0.6911455428069004 1101 30-31 0.6290915968591051 1101 32-33 0.6065843504560746 1101 34-35 0.6099126830386581 1101 36-37 0.6251362187561895 1101 38-39 0.6785844592572019 1101 40-41 0.829710424981478 1101 42-43 0.8093307163896952 1101 44-45 0.9110205012534252 1101 46-47 1.0180179221192844 1101 48-49 0.8764606311453207 1101 50-51 0.9314511378772252 1101 52-53 0.9638940564336167 1101 54-55 1.0625354553404875 1101 56-57 1.035710273079708 1101 58-59 1.0358094034136442 1101 60-61 1.2762201922011656 1101 62-63 1.0239187749086653 1101 64-65 0.915121438964654 1101 66-67 0.8211774166056927 1101 68-69 0.8190795907223603 1101 70-71 0.8240486057825507 1101 72-73 0.8643634480033171 1101 74-75 0.8736009375155369 1101 76-77 0.8337711711920122 1101 78-79 0.8431644541432242 1101 80-81 0.9857640015743243 1101 82-83 0.7293965794966937 1101 84-85 1.011355173044123 1101 86-87 1.090146596609788 1101 88-89 1.1734519020161827 1101 90-91 1.0333613537338984 1101 92-93 0.9451450155504482 1101 94-95 1.084141153212748 1101 96-97 1.2841054061281376 1101 98-99 1.2253162341506005 1101 100-101 1.473268189069593 1102 1 0.35778733492810844 1102 2 0.3532788373149742 1102 3 0.3615456997884259 1102 4 0.30486818509094604 1102 5 0.2520487536809455 1102 6 0.22593447760129948 1102 7 0.21877640546974675 1102 8 0.19893312642749095 1102 9 0.2677568139855282 1102 10-11 0.2834937545371048 1102 12-13 0.4308920813704944 1102 14-15 0.6296842066764796 1102 16-17 0.4078448655374558 1102 18-19 0.39271227368142547 1102 20-21 0.4261715450321617 1102 22-23 0.4738855703610696 1102 24-25 0.6430515381388986 1102 26-27 0.449548581985006 1102 28-29 0.6031799187889888 1102 30-31 0.4817250611479551 1102 32-33 0.6257107487745799 1102 34-35 0.5252758016390899 1102 36-37 0.4239135140662569 1102 38-39 0.4197301019035464 1102 40-41 0.5432893299505182 1102 42-43 0.6821954173956684 1102 44-45 0.7744419876965836 1102 46-47 0.5968304129109754 1102 48-49 0.7109044938462716 1102 50-51 0.5625731187391239 1102 52-53 0.7301567907671682 1102 54-55 0.6513457140076753 1102 56-57 0.7489445582592182 1102 58-59 0.6565870996208716 1102 60-61 0.7463550934596839 1102 62-63 0.7950610409134171 1102 64-65 1.0098123655998492 1102 66-67 0.7966360612824559 1102 68-69 0.7321873466910773 1102 70-71 0.763733661234717 1102 72-73 0.8666154909031007 1102 74-75 0.8820469678433476 1102 76-77 0.8861434200037053 1102 78-79 0.8392511225424748 1102 80-81 0.7998281881166989 1102 82-83 1.0016816236933934 1102 84-85 1.092020706575628 1102 86-87 1.0976921891756106 1102 88-89 1.0378394304473169 1102 90-91 0.8849317133968171 1102 92-93 1.0156292474561681 1102 94-95 1.0343883309663688 1102 96-97 0.9784489939552827 1102 98-99 0.9813050305296258 1102 100-101 1.2195707981259467 1103 1 0.17549252755792466 1103 2 0.243368172881695 1103 3 0.22159595104470142 1103 4 0.16275763232712848 1103 5 0.11728046780713441 1103 6 0.10090097676377496 1103 7 0.2946474272452875 1103 8 0.1139331264274901 1103 9 0.25092543486771746 1103 10-11 0.3160691314215285 1103 12-13 0.1302527737211392 1103 14-15 0.1815895668104872 1103 16-17 0.33665837754192296 1103 18-19 0.32208134124144294 1103 20-21 0.2707848895324432 1103 22-23 0.19181410190768844 1103 24-25 0.5687243466257783 1103 26-27 0.4025203854467563 1103 28-29 0.4187326826080806 1103 30-31 0.4067529785125572 1103 32-33 0.5217995259940125 1103 34-35 0.4206931662398645 1103 36-37 0.6370416547697815 1103 38-39 0.6167582984418019 1103 40-41 0.6738365102966952 1103 42-43 0.786635674235427 1103 44-45 0.7982024567083101 1103 46-47 0.7477558735474901 1103 48-49 0.7679633994855806 1103 50-51 0.9722520690462204 1103 52-53 0.8845118996448917 1103 54-55 0.918235719591145 1103 56-57 0.9804255744512957 1103 58-59 1.0047124485879309 1103 60-61 0.8744622961397539 1103 62-63 0.9688284892662864 1103 64-65 1.0015572008873974 1103 66-67 1.0188680545822848 1103 68-69 1.0260636727658934 1103 70-71 0.8286415339315383 1103 72-73 0.8623664680108618 1103 74-75 0.9389523279773506 1103 76-77 0.9301635205062198 1103 78-79 1.0672578227099798 1103 80-81 1.0653223254701345 1103 82-83 0.9847860346370005 1103 84-85 1.061067328574513 1103 86-87 1.2198795146920816 1103 88-89 1.2020633277114143 1103 90-91 1.1870841422075387 1103 92-93 0.9468576114986043 1103 94-95 1.0240156341489488 1103 96-97 1.0866022602869378 1103 98-99 1.1512980511884763 1103 100-101 1.5639426574224302 1104 1 0.28622930711823713 1104 2 0.29390293223998043 1104 3 0.3020772344671663 1104 4 0.28917474462659243 1104 5 0.22055438343399203 1104 6 0.2245819036799972 1104 7 0.2070033393070858 1104 8 0.27974120723557405 1104 9 0.22411913659082927 1104 10-11 0.4171074558422063 1104 12-13 0.3859182520336759 1104 14-15 0.425491824683327 1104 16-17 0.4474337786114404 1104 18-19 0.464980925317505 1104 20-21 0.3880561313625108 1104 22-23 0.41401849881796693 1104 24-25 0.27701905844990193 1104 26-27 0.21295264926137492 1104 28-29 0.400007192412005 1104 30-31 0.2962806077460627 1104 32-33 0.476858943700492 1104 34-35 0.422771300523884 1104 36-37 0.43486548127007296 1104 38-39 0.3654763614245624 1104 40-41 0.39633799573935846 1104 42-43 0.43358606045051573 1104 44-45 0.3405583687701039 1104 46-47 0.34847037146786874 1104 48-49 0.4084847898599051 1104 50-51 0.5031165966754543 1104 52-53 0.6552486792052008 1104 54-55 0.733446652449139 1104 56-57 0.7097348436135036 1104 58-59 0.6185552887543011 1104 60-61 0.6464646728480119 1104 62-63 0.5678614660933832 1104 64-65 0.6371513779521578 1104 66-67 0.535666925645863 1104 68-69 0.7124436489988142 1104 70-71 0.6802458120064045 1104 72-73 0.6799125167333813 1104 74-75 0.4669217278585158 1104 76-77 0.49022293821269614 1104 78-79 0.6470676860492581 1104 80-81 0.6707887544659741 1104 82-83 0.6023617922127578 1104 84-85 0.6844704777129529 1104 86-87 0.6926142146326626 1104 88-89 0.8181506717399323 1104 90-91 0.7637240709062887 1104 92-93 0.8536965895140511 1104 94-95 0.7447568700372464 1104 96-97 0.7350677980171838 1104 98-99 0.6525175996139048 1104 100-101 0.9284643448852918 1105 1 0.2852559086426645 1105 2 0.3206862542728999 1105 3 0.21465774279154815 1105 4 0.27410536235350236 1105 5 0.31200429362139914 1105 6 0.2572216239022538 1105 7 0.23839448732653779 1105 8 0.3114696526907679 1105 9 0.19319220131344395 1105 10-11 0.3907578563876726 1105 12-13 0.3639314138750791 1105 14-15 0.4083045194154238 1105 16-17 0.5666701372169314 1105 18-19 0.43287173394331546 1105 20-21 0.5561670789701125 1105 22-23 0.3989832274154921 1105 24-25 0.48159014378920517 1105 26-27 0.532719765391171 1105 28-29 0.46300215734260064 1105 30-31 0.41603777645995876 1105 32-33 0.4539002315745151 1105 34-35 0.46542322824542026 1105 36-37 0.475455702236097 1105 38-39 0.4826996069729077 1105 40-41 0.44461478696977963 1105 42-43 0.6146484673364156 1105 44-45 0.5534761364175225 1105 46-47 0.5336508560861546 1105 48-49 0.3854609050090616 1105 50-51 0.33052054599013303 1105 52-53 0.16407500990074908 1105 54-55 0.18111790902882063 1105 56-57 0.28076892132607156 1105 58-59 0.4338912312833898 1105 60-61 0.2679096626852626 1105 62-63 0.17940382245708264 1105 64-65 0.4312617835971082 1105 66-67 0.6067293973233561 1105 68-69 0.6144656042034242 1105 70-71 0.7815850162207525 1105 72-73 0.5076882554814688 1105 74-75 0.4697329209500545 1105 76-77 0.3871558945351552 1105 78-79 0.32637976697624893 1105 80-81 0.3135583742193333 1105 82-83 0.2997072804498444 1105 84-85 0.3001762659275151 1105 86-87 0.5842984798406796 1105 88-89 0.5744797627481191 1105 90-91 0.37211336321815836 1105 92-93 0.17577714681326384 1105 94-95 0.39410169358876246 1105 96-97 0.4686632324200737 1105 98-99 0.6745139659486483 1105 100-101 0.6753338626109411 1106 1 0.6499737029481913 1106 2 0.573500070368965 1106 3 0.562680348740674 1106 4 0.36092776338982446 1106 5 0.5124328177528739 1106 6 0.5444701394651688 1106 7 0.5875712940121289 1106 8 0.5533867536083932 1106 9 0.7280107508373277 1106 10-11 0.7380048105266255 1106 12-13 0.7066952981388681 1106 14-15 0.999569990885945 1106 16-17 0.9261612575819953 1106 18-19 0.9339437671534583 1106 20-21 0.8536405118715393 1106 22-23 1.0088363909134515 1106 24-25 1.0015050461833468 1106 26-27 0.9858493361104195 1106 28-29 0.8725940232876042 1106 30-31 0.8168018139746138 1106 32-33 0.7970098941382631 1106 34-35 0.9075633139984447 1106 36-37 0.9229451108178566 1106 38-39 0.9355205073073094 1106 40-41 0.8474828914637342 1106 42-43 0.9270378363524614 1106 44-45 0.9499016803496829 1106 46-47 0.6596669262230108 1106 48-49 0.7377620053792242 1106 50-51 0.471941942992288 1106 52-53 0.38196160329293605 1106 54-55 0.5086439339663045 1106 56-57 0.5686889937684327 1106 58-59 0.6290147693158659 1106 60-61 0.7070977415024728 1106 62-63 0.7837122441707081 1106 64-65 0.6025810760021741 1106 66-67 0.669781528161046 1106 68-69 0.7259599495836611 1106 70-71 0.7296975486239106 1106 72-73 0.7111092679106861 1106 74-75 0.7457970145121173 1106 76-77 0.842638685378077 1106 78-79 0.772505339197167 1106 80-81 0.9150593652898209 1106 82-83 0.9739287009697506 1106 84-85 1.20120319133434 1106 86-87 1.2507429006174497 1106 88-89 1.2961759414521268 1106 90-91 1.3609137607065769 1106 92-93 1.1686398971651855 1106 94-95 1.184116435551406 1106 96-97 0.9357155001233224 1106 98-99 1.3195451920182606 1106 100-101 1.4452240665550775 1107 1 0.3086769490928667 1107 2 0.47423873416462925 1107 3 0.3977127895326795 1107 4 0.4829867274015882 1107 5 0.4239356797200813 1107 6 0.4286272081498055 1107 7 0.40054089803567194 1107 8 0.4562068950414684 1107 9 0.37593688962143545 1107 10-11 0.5735977683058309 1107 12-13 0.5145998527589342 1107 14-15 0.5312602426409541 1107 16-17 0.5833765906003379 1107 18-19 0.6304433114590893 1107 20-21 0.5695735493262575 1107 22-23 0.796934376821774 1107 24-25 0.7717713111160478 1107 26-27 0.6608623098453847 1107 28-29 0.5806284443492089 1107 30-31 0.6310656932891945 1107 32-33 0.5383115534854213 1107 34-35 0.5834337160680505 1107 36-37 0.6364832355257946 1107 38-39 0.7238802915689462 1107 40-41 0.7738021460355284 1107 42-43 0.7263750785882266 1107 44-45 0.6856423192512651 1107 46-47 0.8484746593435943 1107 48-49 0.6446787488555685 1107 50-51 0.7048923897793244 1107 52-53 0.8349700897937993 1107 54-55 0.9515690529244836 1107 56-57 0.8588935011408694 1107 58-59 0.9101792298021323 1107 60-61 0.9594336592554527 1107 62-63 0.8406469314197764 1107 64-65 1.0099821722505098 1107 66-67 0.9382237246853791 1107 68-69 0.9998924241977392 1107 70-71 0.9076220608502084 1107 72-73 0.8654936157771829 1107 74-75 0.9557879522614279 1107 76-77 0.8299115159243229 1107 78-79 0.9497549590215506 1107 80-81 1.0182432876694492 1107 82-83 1.058989929253265 1107 84-85 1.1342202495367104 1107 86-87 1.0700942913243843 1107 88-89 0.8725386999909084 1107 90-91 0.9124392395729437 1107 92-93 1.1018518841217393 1107 94-95 1.0296084176541065 1107 96-97 0.9982202442502839 1107 98-99 0.9380993112113849 1107 100-101 1.10127369980502 1108 1 0.21159402186181708 1108 2 0.18228665194664728 1108 3 0.29525329680591383 1108 4 0.24923196284939308 1108 5 0.21642679122776087 1108 6 0.22781546115496099 1108 7 0.15463189240286113 1108 8 0.17015668020763286 1108 9 0.1951634878341295 1108 10-11 0.24589861803197266 1108 12-13 0.07252307997946161 1108 14-15 0.4708834712342167 1108 16-17 0.46796552356943977 1108 18-19 0.44950403690648244 1108 20-21 0.5375148052004377 1108 22-23 0.45536048450866673 1108 24-25 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2316 58-59 -1.3808619606629478 2316 60-61 -1.2057023594660734 2316 62-63 -0.9534289265205373 2316 64-65 -1.1474422963473998 2316 66-67 -1.2128476316922203 2316 68-69 -0.7642291879681409 2316 70-71 -1.2732941121217536 2316 72-73 -1.0558386601942615 2316 74-75 -1.4547983004791618 2316 76-77 -1.7245863538024793 2316 78-79 -1.9539852642684004 2316 80-81 -1.6218948691000037 2316 82-83 -1.8891254786862781 2316 84-85 -1.5052976789669685 2316 86-87 -1.3801581926985662 2316 88-89 -1.8796988643448955 2316 90-91 -1.7489050483404789 2316 92-93 -1.9935999048412647 2316 94-95 -2.0124361003910174 2316 96-97 -1.958425391799537 2316 98-99 -2.3728749000432963 2316 100-101 -2.2048255678163855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3249.0 3 907.0 4 924.0 5 985.0 6 1127.0 7 1357.0 8 1831.0 9 2182.0 10 2884.0 11 3794.0 12 5410.0 13 7048.0 14 8295.0 15 10550.0 16 14435.0 17 20214.0 18 17193.0 19 9857.0 20 9092.0 21 9100.0 22 9817.0 23 10509.0 24 12147.0 25 13692.0 26 15926.0 27 18638.0 28 22548.0 29 27047.0 30 33510.0 31 42267.0 32 54545.0 33 71563.0 34 99791.0 35 143975.0 36 216022.0 37 310344.0 38 279569.0 39 51109.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25988108334341 19.382736670192823 15.08779878753903 21.269583458924732 2 15.124862039737677 21.13730471100686 40.62151898410032 23.116314265155143 3 18.39762630267585 27.1629875254167 30.69927684409875 23.7401093278087 4 12.185598831922333 17.358761222895346 38.60964176389974 31.84599818128258 5 12.791815123198912 38.47090572089852 33.88919300469619 14.84808615120638 6 29.218153101734273 37.54872818555411 18.16731445516943 15.065804257542185 7 26.609714665865873 34.56370059092716 21.627652497177905 17.19893224602906 8 26.2026796208992 33.53002409712602 20.515143289552192 19.752152992422594 9 27.284992108186675 16.313866860466977 21.16581132002984 35.23532971131651 10-11 24.239565332361828 27.250186799262664 27.436825994005776 21.073421874369732 12-13 26.097289836623265 25.12998022808917 28.046441104064524 20.726288831223037 14-15 23.226382819632 25.52587541339588 27.571600871156203 23.676140895815916 16-17 20.948585979170087 29.15845119908211 27.778604793511246 22.11435802823656 18-19 20.953761526970975 27.024043442649447 31.34583937601443 20.67635565436515 20-21 22.329508924385383 25.786070130983152 31.26381509194487 20.620605852686598 22-23 21.902457679869556 25.915649367378258 31.28375729471196 20.898135658040225 24-25 21.651572076282427 26.10868681063566 31.334520079703786 20.905221033378123 26-27 21.200650816480312 26.220096918540754 31.47599994240562 21.10325232257332 28-29 21.047270539555857 26.355593885855 31.483814604377443 21.113320970211706 30-31 21.262059536748694 26.130796040610075 31.297891394818826 21.309253027822404 32-33 21.095541971885964 26.366435929143794 31.212147874649855 21.325874224320383 34-35 21.57264553646786 26.285443889666343 31.087761799912595 21.054148773953198 36-37 21.597650269639878 26.033534210109536 30.98143796203125 21.387377558219338 38-39 21.308192923740474 26.515783680704423 30.944653130179372 21.23137026537573 40-41 21.354771178079844 26.45112339720974 30.931175980602514 21.262929444107908 42-43 21.30495748550744 26.551142188993975 30.36586271204447 21.778037613454114 44-45 21.440062404133315 26.53481084546978 30.248279118995953 21.77684763140095 46-47 21.777787730801286 26.847793921226444 29.736307083477964 21.638111264494313 48-49 21.806648161159167 27.101994013225166 29.195509534943543 21.895848290672124 50-51 21.66030348154463 27.38371525772899 29.088675663096137 21.867305597630242 52-53 21.863099562878137 27.565903407042256 28.618796655952174 21.95220037412743 54-55 21.632778298048922 27.437372560675755 28.720332413269272 22.209516728006047 56-57 21.77503062074685 27.662053183469787 28.365402459642684 22.19751373614067 58-59 22.089184977553362 27.112367568051866 28.73831671034401 22.060130744050763 60-61 21.963127132009657 27.330583925467135 29.11439266702269 21.591896275500524 62-63 21.71816790707825 27.370099179593975 29.25935620622853 21.65237670709924 64-65 21.570327494424344 27.545517863375863 29.005346423341642 21.878808218858147 66-67 21.717239918362594 27.587440536723072 28.568132611507448 22.12718693340689 68-69 21.50135123979371 27.4839870245143 28.992684810759233 22.02197692493276 70-71 21.900546484445474 27.39114134767082 28.69273652949749 22.015575638386213 72-73 21.68742063327053 27.6121689787641 28.488121779350095 22.212288608615268 74-75 21.496595027024814 28.145441354106953 28.232293116861847 22.125670502006386 76-77 21.519433634117092 27.659543155068512 28.4942916779178 22.3267315328966 78-79 21.46013706204912 27.751416956372594 28.493495056652655 22.294950924925633 80-81 21.59488420811506 28.102622969490938 28.115868028028334 22.186624794365667 82-83 21.76718634704649 27.8172464086248 28.24161329815182 22.17395394617689 84-85 21.574082518532027 27.862892580628127 28.03620485792777 22.526820042912068 86-87 21.491653903730445 27.88113284952578 28.172915011965056 22.45429823477872 88-89 21.603764073264998 27.681526114058464 27.99257427723232 22.722135535444224 90-91 21.537991882333515 27.560898498449298 28.215066962428594 22.686042656788594 92-93 21.674114915101487 27.675480818466706 28.21160108965037 22.438803176781434 94-95 21.480553780999433 27.380095838833036 28.37301866719242 22.766331712975113 96-97 21.264382716701412 27.507522848622134 28.392307793258258 22.835786641418192 98-99 21.132338504708926 27.717309518579874 28.023340029418375 23.127011947292825 100-101 21.16055914869448 27.51820057111005 28.307374901189124 23.013865379006344 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 906.0 1 682.0 2 549.5 3 636.5 4 592.5 5 643.5 6 1023.5 7 2065.5 8 3520.5 9 4240.5 10 3781.5 11 3244.5 12 3260.5 13 3439.5 14 3600.0 15 3742.0 16 3856.5 17 4012.0 18 4114.0 19 4195.0 20 4489.5 21 4932.0 22 5481.5 23 6261.0 24 7117.0 25 8410.5 26 10110.5 27 11978.5 28 13824.5 29 16237.5 30 19490.0 31 22684.0 32 26275.5 33 30329.5 34 34006.5 35 37038.5 36 42118.0 37 47254.5 38 48797.0 39 48820.5 40 48316.0 41 48049.5 42 47545.5 43 48876.0 44 51528.0 45 52804.0 46 52742.5 47 53827.5 48 59808.5 49 60092.0 50 53665.5 51 49514.0 52 47384.0 53 47235.5 54 46178.0 55 42828.5 56 39605.0 57 36090.0 58 33418.5 59 30724.0 60 26549.5 61 22191.5 62 17903.5 63 13675.5 64 10022.5 65 7529.0 66 5370.0 67 3986.5 68 2830.0 69 1711.0 70 1153.0 71 870.5 72 616.0 73 446.0 74 317.5 75 241.0 76 188.5 77 99.5 78 56.5 79 37.0 80 29.0 81 21.0 82 14.5 83 12.0 84 9.0 85 8.5 86 8.0 87 5.5 88 7.0 89 7.0 90 5.0 91 4.0 92 1.5 93 0.5 94 0.5 95 0.0 96 0.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055325583061289235 2 0.09133518220985089 3 0.06491961480602147 4 0.12357112887215121 5 0.1002256516266361 6 0.09536467554263843 7 0.108028797445685 8 0.09338190898206045 9 0.11045928548768383 10-11 0.10457494601758138 12-13 0.09002399787140417 14-15 0.09018389840048303 16-17 0.10435108527687097 18-19 0.08445945945945946 20-21 0.0700684118423611 22-23 0.07192325797967601 24-25 0.05989873819294494 26-27 0.052415393432053786 28-29 0.06584703787467892 30-31 0.04838590009926625 32-33 0.04665897438521444 34-35 0.04381274496761055 36-37 0.04729857650152993 38-39 0.05001688549587073 40-41 0.06405615194899557 42-43 0.06050636020344464 44-45 0.04828995978181893 46-47 0.03674514158232447 48-49 0.04438838687229448 50-51 0.028430314070223194 52-53 0.04064671449184891 54-55 0.045955412057267414 56-57 0.05155193057502789 58-59 0.05609310560086781 60-61 0.05324687618326391 62-63 0.06095408168486548 64-65 0.04167007787795368 66-67 0.06095408168486548 68-69 0.05327885628907969 70-71 0.024944482536303814 72-73 0.020275387087200794 74-75 0.029677538197038385 76-77 0.04259750094661113 78-79 0.0619134848593387 80-81 0.039975132269717654 82-83 0.058139832373077356 84-85 0.06232922623494377 86-87 0.064280012689706 88-89 0.08535490242230113 90-91 0.061018041896497024 92-93 0.0726588004134388 94-95 0.08097362792554008 96-97 0.08938439575508868 98-99 0.11915787426957439 100-101 0.11362531596344545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1563472.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.65315242870378 #Duplication Level Percentage of deduplicated Percentage of total 1 70.64428705263276 32.251344050309484 2 14.909699661691336 13.613495826427757 3 5.670948378846434 7.766900122643607 4 2.758400497093376 5.037187134128648 5 1.5487324492680157 3.5352259288856236 6 0.9429322171141227 2.582869694270799 7 0.6531262480734932 2.087209051094061 8 0.45766594885396594 1.6715114659565964 9 0.33801039467605876 1.3888116063569191 >10 1.8363232115153973 15.350506186040528 >50 0.15887837246991823 4.978821782448248 >100 0.07620634490257158 6.05725371412876 >500 0.0032397684070562737 0.9895530445621507 >1k 0.0011268759676717473 0.8815176198893918 >5k 2.817189919179368E-4 0.9742480260668432 >10k+ 1.408594959589684E-4 0.8335447467906549 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12962 0.8290522631681283 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9116 0.5830612892331938 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6034 0.38593591698476215 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3034 0.19405528209011738 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2126 0.13597940992867158 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1956 0.12510617395130838 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1746 0.11167452950868323 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.396021163154824E-5 0.0 0.0 0.0 0.0 7 6.396021163154824E-5 0.0 0.0 0.0 0.0 8 6.396021163154824E-5 0.0 0.0 0.0 0.0 9 6.396021163154824E-5 0.0 0.0 0.0 0.0 10-11 6.396021163154824E-5 0.0 0.0 0.0 0.0 12-13 6.396021163154824E-5 0.0 0.0 0.0 0.0 14-15 6.396021163154824E-5 0.0 0.0 0.0 0.0 16-17 1.2792042326309648E-4 0.0 0.0 6.396021163154824E-5 0.0 18-19 1.2792042326309648E-4 0.0 0.0 9.594031744732237E-5 0.0 20-21 1.2792042326309648E-4 0.0 0.0 2.878209523419671E-4 0.0 22-23 1.2792042326309648E-4 0.0 0.0 5.116816930523859E-4 0.0 24-25 1.2792042326309648E-4 0.0 0.0 7.355424337628048E-4 0.0 26-27 1.2792042326309648E-4 0.0 0.0 0.0011832639151836426 0.0 28-29 1.2792042326309648E-4 0.0 0.0 0.0021746471954726403 0.0 30-31 1.2792042326309648E-4 0.0 0.0 0.0043492943909452806 0.0 32-33 1.2792042326309648E-4 0.0 0.0 0.007003643173654533 0.0 34-35 1.2792042326309648E-4 0.0 0.0 0.010265613966863494 0.0 36-37 1.2792042326309648E-4 0.0 0.0 0.014934709415966516 0.0 38-39 1.2792042326309648E-4 0.0 0.0 0.021266770367489793 6.396021163154824E-5 40-41 1.2792042326309648E-4 0.0 0.0 0.029453677456327966 6.396021163154824E-5 42-43 1.2792042326309648E-4 0.0 0.0 0.03661722115906137 6.396021163154824E-5 44-45 1.2792042326309648E-4 0.0 0.0 0.04128631660816439 6.396021163154824E-5 46-47 1.2792042326309648E-4 0.0 0.0 0.049409263485371016 6.396021163154824E-5 48-49 1.2792042326309648E-4 0.0 0.0 0.056157065812499365 6.396021163154824E-5 50-51 1.599005290788706E-4 0.0 0.0 0.06223328591749644 6.396021163154824E-5 52-53 1.9188063489464474E-4 0.0 0.0 0.07672027385204212 6.396021163154824E-5 54-55 1.9188063489464474E-4 0.0 0.0 0.1041911847477921 6.396021163154824E-5 56-57 1.9188063489464474E-4 0.0 0.0 0.1473323474932714 6.396021163154824E-5 58-59 1.9188063489464474E-4 0.0 0.0 0.2035533735174023 6.396021163154824E-5 60-61 2.2386074071041885E-4 0.0 0.0 0.27026387424910714 6.396021163154824E-5 62-63 3.1980105815774125E-4 0.0 0.0 0.3087679216512992 6.396021163154824E-5 64-65 3.1980105815774125E-4 0.0 0.0 0.353667990216646 6.396021163154824E-5 66-67 3.1980105815774125E-4 0.0 0.0 0.4461544562358648 6.396021163154824E-5 68-69 3.1980105815774125E-4 0.0 0.0 0.570013406060358 6.396021163154824E-5 70-71 3.1980105815774125E-4 0.0 0.0 0.7032425268888729 6.396021163154824E-5 72-73 3.1980105815774125E-4 0.0 0.0 0.8504149738530655 6.396021163154824E-5 74-75 3.1980105815774125E-4 0.0 0.0 0.9842517166920801 6.396021163154824E-5 76-77 3.1980105815774125E-4 0.0 0.0 1.106063939744364 6.396021163154824E-5 78-79 3.1980105815774125E-4 0.0 0.0 1.2168430262902055 6.396021163154824E-5 80-81 3.1980105815774125E-4 0.0 0.0 1.3281337945290992 6.396021163154824E-5 82-83 3.1980105815774125E-4 0.0 0.0 1.4882901644544964 6.396021163154824E-5 84-85 3.1980105815774125E-4 0.0 0.0 1.7704186579612555 6.396021163154824E-5 86-87 3.1980105815774125E-4 0.0 0.0 2.1365588894460537 6.396021163154824E-5 88-89 3.1980105815774125E-4 0.0 0.0 2.557832823357246 6.396021163154824E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13510 0.0 34.551212 1 GGTATCA 8670 0.0 33.443977 1 TATCAAC 16785 0.0 27.809767 2 ATCAACG 16830 0.0 27.535055 3 TCAACGC 16855 0.0 27.522411 4 CAACGCA 16955 0.0 27.385487 5 AACGCAG 17255 0.0 26.9369 6 GTACATG 10505 0.0 25.882553 1 GAGTACT 8940 0.0 25.114147 12-13 TACATGG 10875 0.0 24.739697 2 ACGCAGA 19065 0.0 24.079647 7 CGCAGAG 19095 0.0 23.843475 8 ACATGGG 10465 0.0 23.617262 3 GTACTTT 9530 0.0 23.609955 14-15 AGTACTT 9490 0.0 23.458353 12-13 GCAGAGT 19530 0.0 22.681156 9 AGAGTAC 14895 0.0 22.650923 10-11 CATGGGA 6730 0.0 21.893343 4 GTATAGG 555 0.0 21.41185 1 TACTTTT 10525 0.0 20.83616 14-15 >>END_MODULE