FastQCFastQC Report
Thu 26 May 2016
SRR937047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563472
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141960.907979164321459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110340.7057369751425033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75450.48257979676003154No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39170.2505321489607745No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23760.15196946283655863No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18570.1187741129997851No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16820.10758107596426415No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15950.10201653755231946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA154550.031.4768051
GGTATCA103350.031.3497161
GTACATG98400.026.4089981
ATCAACG190000.025.5489863
TCAACGC191350.025.3687324
TATCAAC191800.025.3100222
CAACGCA193200.025.1258135
AACGCAG196750.024.6724626
TACATGG102900.024.511472
ACATGGG99450.024.1669643
GAGTACT110500.024.13553212-13
GTACTTT115700.023.07242214-15
AGTACTT115350.022.58543412-13
ACGCAGA216100.022.2654257
CGCAGAG216650.022.1431338
CATGGGA64950.021.8659524
AGAGTAC166700.021.81152210-11
CCTATAC4250.021.234453
GCAGAGT221300.021.2271299
TACTTTT124550.020.61304714-15
Produced by FastQC (version 0.11.5)