##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937047_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1563472 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.150582165846274 33.0 31.0 34.0 31.0 34.0 2 32.120562440517006 33.0 31.0 34.0 30.0 34.0 3 32.294686441458495 33.0 31.0 34.0 30.0 34.0 4 36.04534779004677 37.0 35.0 37.0 35.0 37.0 5 35.98940818895382 37.0 35.0 37.0 35.0 37.0 6 36.047170656078265 37.0 35.0 37.0 35.0 37.0 7 35.98310235168906 37.0 35.0 37.0 35.0 37.0 8 35.971292738213414 37.0 35.0 37.0 35.0 37.0 9 37.64706883142135 39.0 37.0 39.0 35.0 39.0 10-11 37.578676816725846 39.0 37.0 39.0 35.0 39.0 12-13 37.481374146770776 39.0 37.0 39.0 35.0 39.0 14-15 38.63581439258266 40.0 38.0 41.0 34.0 41.0 16-17 38.571142303795654 40.0 38.0 41.0 34.0 41.0 18-19 38.68668386769958 40.0 38.0 41.0 34.5 41.0 20-21 38.77989404351341 40.0 38.0 41.0 35.0 41.0 22-23 38.70064350368922 40.0 38.0 41.0 34.0 41.0 24-25 38.59164474963415 40.0 38.0 41.0 34.0 41.0 26-27 38.42242233951104 40.0 38.0 41.0 34.0 41.0 28-29 38.24171491398631 40.0 38.0 41.0 34.0 41.0 30-31 38.04120060992458 40.0 37.0 41.0 33.5 41.0 32-33 37.936631100524984 40.0 37.0 41.0 33.0 41.0 34-35 37.80724822702293 40.0 37.0 41.0 33.0 41.0 36-37 37.612803747045035 40.0 36.5 41.0 33.0 41.0 38-39 37.428481290358896 40.0 36.0 41.0 33.0 41.0 40-41 37.21976888617129 40.0 36.0 41.0 32.5 41.0 42-43 36.92975921538729 40.0 36.0 41.0 31.5 41.0 44-45 36.47690940419784 39.0 35.0 41.0 30.5 41.0 46-47 36.04149258829068 39.0 35.0 41.0 29.5 41.0 48-49 35.58294839945967 39.0 35.0 41.0 27.5 41.0 50-51 35.26141785717941 39.0 35.0 40.0 26.5 41.0 52-53 34.99879690841921 39.0 35.0 40.0 26.0 41.0 54-55 34.75696750565408 38.0 34.5 40.0 25.5 41.0 56-57 34.47364519479722 38.0 34.0 40.0 24.5 41.0 58-59 34.14536173337291 38.0 34.0 40.0 23.0 41.0 60-61 33.807211769702306 37.0 33.5 40.0 22.5 41.0 62-63 33.43893526714901 37.0 33.0 40.0 22.0 41.0 64-65 33.04659373496935 36.0 33.0 39.0 20.5 40.5 66-67 32.665293654123644 36.0 32.0 39.0 19.5 40.0 68-69 32.22167362127368 35.0 32.0 38.5 18.0 40.0 70-71 31.750175250979872 35.0 31.0 38.0 16.0 40.0 72-73 31.422194960958684 35.0 31.0 37.0 12.5 39.5 74-75 30.98949357583634 35.0 31.0 37.0 8.0 39.0 76-77 29.60865272931015 33.5 29.5 35.0 7.0 37.5 78-79 30.325749038038417 34.0 30.5 36.0 7.0 38.0 80-81 30.16264666076527 34.0 30.0 35.5 2.0 37.0 82-83 29.842568015289046 34.0 30.0 35.0 2.0 37.0 84-85 29.47039025962729 34.0 30.0 35.0 2.0 36.0 86-87 29.137919323147457 34.0 29.0 35.0 2.0 36.0 88-89 28.79946299006314 34.0 29.0 35.0 2.0 36.0 90-91 28.396077767942117 34.0 29.0 35.0 2.0 35.0 92-93 27.96541543436659 34.0 28.5 35.0 2.0 35.0 94-95 27.52486389266965 33.0 27.0 35.0 2.0 35.0 96-97 26.937527502891 33.0 26.0 35.0 2.0 35.0 98-99 26.27309123540428 33.0 25.0 35.0 2.0 35.0 100-101 23.382230062322833 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2575733525234867 1101 2 0.19395733276314786 1101 3 0.19745089670466598 1101 4 0.16982762403400642 1101 5 0.18549553372665173 1101 6 0.18558187564290307 1101 7 0.29226808258128045 1101 8 0.23522455258541441 1101 9 0.29045405639906363 1101 10-11 0.3706089056021682 1101 12-13 0.2934520025832299 1101 14-15 0.39579725583173797 1101 16-17 0.3567564088484616 1101 18-19 0.33752949772527074 1101 20-21 0.37802876094057325 1101 22-23 0.31064860217424695 1101 24-25 0.4177478430516288 1101 26-27 0.4433330335966872 1101 28-29 0.40509523985695495 1101 30-31 0.443766380724945 1101 32-33 0.48868019695873244 1101 34-35 0.5490422968405468 1101 36-37 0.6577625848539412 1101 38-39 0.6033221406843481 1101 40-41 0.566176068160182 1101 42-43 0.6609506849487872 1101 44-45 0.771850042249973 1101 46-47 0.8189858056903745 1101 48-49 0.453616125364249 1101 50-51 0.3540764309845983 1101 52-53 0.17039064333231835 1101 54-55 0.2876124455329432 1101 56-57 0.3771235426295405 1101 58-59 0.47960880793196026 1101 60-61 0.34260735160088984 1101 62-63 0.5079481240307757 1101 64-65 0.4146900960752333 1101 66-67 0.41130023620633693 1101 68-69 0.3197310502619146 1101 70-71 0.28683413638325916 1101 72-73 0.5051507216645597 1101 74-75 0.3804545223741407 1101 76-77 0.2030110989687195 1101 78-79 0.37829615845440756 1101 80-81 0.5349519320501912 1101 82-83 0.6214046572967717 1101 84-85 0.6614437007448082 1101 86-87 0.4440947186982491 1101 88-89 0.5234270995986492 1101 90-91 0.7534913915459178 1101 92-93 0.7667849071154329 1101 94-95 0.762250019435168 1101 96-97 0.7865497986783581 1101 98-99 0.8438073738662233 1101 100-101 0.8803070465451697 1102 1 0.2049337862512033 1102 2 0.07670534993469857 1102 3 0.19046365143086064 1102 4 0.16282868065724898 1102 5 0.18973751878722567 1102 6 0.12584535486106319 1102 7 0.184510849201601 1102 8 0.21562074520068109 1102 9 0.27500497255022793 1102 10-11 0.26397927449710323 1102 12-13 0.242693617730815 1102 14-15 0.33938157354462106 1102 16-17 0.3230320427117448 1102 18-19 0.35894793380904844 1102 20-21 0.35057932045767615 1102 22-23 0.3568056805066462 1102 24-25 0.35573035027757527 1102 26-27 0.38733042080959024 1102 28-29 0.21243032980270726 1102 30-31 0.3399928256461706 1102 32-33 0.3676416646722913 1102 34-35 0.2966595197362025 1102 36-37 0.32903419393749544 1102 38-39 0.4353437056628877 1102 40-41 0.4071403585943614 1102 42-43 0.5366164492807073 1102 44-45 0.5141251841606049 1102 46-47 0.450170387211287 1102 48-49 0.2659967335770759 1102 50-51 0.3979836795718157 1102 52-53 0.5388672910733447 1102 54-55 0.3176956980861405 1102 56-57 0.45989141495960695 1102 58-59 0.4701123195760033 1102 60-61 0.44920220456205584 1102 62-63 0.4001597484040573 1102 64-65 0.40746066845392903 1102 66-67 0.3726579243576751 1102 68-69 0.1754177194199542 1102 70-71 0.22070270535116876 1102 72-73 0.23615015699396835 1102 74-75 0.18545109830779438 1102 76-77 0.27681664361658065 1102 78-79 0.3014919473902786 1102 80-81 0.3632602978378543 1102 82-83 0.3734880261986575 1102 84-85 0.28858674577997334 1102 86-87 0.14895110709550252 1102 88-89 0.4423214644643778 1102 90-91 0.4604569162880381 1102 92-93 0.21231569430923614 1102 94-95 0.36521466650340173 1102 96-97 0.5270722802190662 1102 98-99 0.48616522452634214 1102 100-101 0.7336958288864679 1103 1 -0.08741406411171937 1103 2 -0.22041916151141905 1103 3 -0.08533757693318478 1103 4 0.09231220941213536 1103 5 0.1343913434661772 1103 6 0.05050197127647493 1103 7 0.1503176610385637 1103 8 0.1652522359879569 1103 9 0.17923724502370675 1103 10-11 0.1594399110130169 1103 12-13 0.21962131175650512 1103 14-15 0.28028888789415163 1103 16-17 0.3054650410367046 1103 18-19 0.22750181432272143 1103 20-21 0.1798841780791136 1103 22-23 0.28289724946253614 1103 24-25 0.38509243849645003 1103 26-27 0.2824169646398502 1103 28-29 0.19896997246044634 1103 30-31 0.19831778377011489 1103 32-33 0.35664641062426483 1103 34-35 0.3605623673073879 1103 36-37 0.524327326265805 1103 38-39 0.5457359446355312 1103 40-41 0.45174672375349445 1103 42-43 0.5578127083538504 1103 44-45 0.5681634309501007 1103 46-47 0.5945492258489224 1103 48-49 0.6045073421756229 1103 50-51 0.42486949755059555 1103 52-53 0.584724354166589 1103 54-55 0.6661099917768141 1103 56-57 0.6707805830221432 1103 58-59 0.6484470487775624 1103 60-61 0.6307292844057244 1103 62-63 0.6432111163549195 1103 64-65 0.48566279017362746 1103 66-67 0.554909459812734 1103 68-69 0.5325129176332482 1103 70-71 0.5986228616884084 1103 72-73 0.6392280464412075 1103 74-75 0.5898536667053378 1103 76-77 0.6123847619862097 1103 78-79 0.6574313667090941 1103 80-81 0.8027410348562825 1103 82-83 0.8539905387614759 1103 84-85 0.8160825581752853 1103 86-87 0.7022286056996343 1103 88-89 0.8541305096905063 1103 90-91 1.1635571396269526 1103 92-93 0.9623882794571976 1103 94-95 0.9728040020701272 1103 96-97 1.1973025984770196 1103 98-99 1.3059349062683872 1103 100-101 1.36513496903164 1104 1 0.21605295906118727 1104 2 0.018410309670990443 1104 3 0.0599268318606363 1104 4 0.06963544173537173 1104 5 0.08663436188566465 1104 6 0.06905217923844731 1104 7 0.04759633008193731 1104 8 0.11874599712877654 1104 9 0.05907087544557044 1104 10-11 0.1255658765507519 1104 12-13 0.10208863201794571 1104 14-15 0.1943441463611677 1104 16-17 0.20862160669327068 1104 18-19 0.09616491592700527 1104 20-21 0.01933456429420488 1104 22-23 0.0575912482741856 1104 24-25 0.11228346642277387 1104 26-27 0.06209610902487839 1104 28-29 0.18078666883597094 1104 30-31 0.20462794419793084 1104 32-33 0.21285704639372227 1104 34-35 0.0858446014131573 1104 36-37 -0.010735062325998967 1104 38-39 -0.04176405536447447 1104 40-41 0.022412202066028897 1104 42-43 0.07503641601873312 1104 44-45 0.08232118496080432 1104 46-47 0.09004982002598183 1104 48-49 0.0753674134768687 1104 50-51 0.29386385286847627 1104 52-53 0.19245459777918938 1104 54-55 0.0858188450150763 1104 56-57 -0.06191846629455 1104 58-59 -0.13715455550051558 1104 60-61 -0.13011147614092522 1104 62-63 0.033012067038228565 1104 64-65 -0.08134849919059661 1104 66-67 0.164022650543572 1104 68-69 -0.060028674761291256 1104 70-71 -0.05147517752727637 1104 72-73 0.08821051821779591 1104 74-75 0.09626780811948166 1104 76-77 0.033042813085433664 1104 78-79 0.1284132443086179 1104 80-81 0.28258060704879284 1104 82-83 0.262760592237413 1104 84-85 0.2621996110570457 1104 86-87 0.2833575421226868 1104 88-89 0.3590918881812968 1104 90-91 0.44294959357823416 1104 92-93 0.3828042034025323 1104 94-95 0.3245553389685192 1104 96-97 0.4673724142821314 1104 98-99 0.6462810144086113 1104 100-101 0.8215832756270061 1105 1 0.17168329174317165 1105 2 -0.07273546113368212 1105 3 0.04582627453855537 1105 4 0.11583833014479694 1105 5 0.1408858912532196 1105 6 0.12116977827525943 1105 7 0.18187920333775764 1105 8 0.07699124247358924 1105 9 0.2243744412321007 1105 10-11 0.2865819536329326 1105 12-13 0.22494684094187534 1105 14-15 0.27496032970157813 1105 16-17 0.2789852819318668 1105 18-19 0.26789237807441424 1105 20-21 0.20706078701646646 1105 22-23 0.1899010268126844 1105 24-25 0.22290959336780958 1105 26-27 0.22396781632540552 1105 28-29 0.21018852423865297 1105 30-31 0.22460529000630913 1105 32-33 0.23195055858236202 1105 34-35 0.1302901130133307 1105 36-37 0.15193262881017233 1105 38-39 0.22040542649712336 1105 40-41 -0.03471111959338202 1105 42-43 0.061022565099627 1105 44-45 0.12057719333340344 1105 46-47 -0.23196937581881372 1105 48-49 -0.23785403620082946 1105 50-51 -0.2246032770200017 1105 52-53 -0.04690061910687149 1105 54-55 -0.15951968536665362 1105 56-57 -0.10039057370224924 1105 58-59 -0.02365739851771309 1105 60-61 0.14101258566721242 1105 62-63 -0.1338186748217538 1105 64-65 -0.029871384154638747 1105 66-67 0.04971293069863236 1105 68-69 -0.2128977801074754 1105 70-71 -0.2444072145713001 1105 72-73 -0.18647307963676596 1105 74-75 -0.21056576170318664 1105 76-77 -0.16408181202847416 1105 78-79 -0.1316419352651046 1105 80-81 0.028561717630140038 1105 82-83 0.09383231767958478 1105 84-85 0.11965714302312236 1105 86-87 0.21856956500403157 1105 88-89 0.20424454290267846 1105 90-91 0.30949452826639856 1105 92-93 0.4802359025774301 1105 94-95 0.32157511603206856 1105 96-97 0.49833727170059916 1105 98-99 0.3743186636627236 1105 100-101 0.5058799266255392 1106 1 0.38817279622720235 1106 2 0.2534392573883224 1106 3 0.20176819149489944 1106 4 0.17532039039552672 1106 5 0.1543951000401833 1106 6 0.2353137251791324 1106 7 0.22140539791142544 1106 8 0.3208153046915214 1106 9 0.3842526887168347 1106 10-11 0.37559902279195967 1106 12-13 0.4686615070983535 1106 14-15 0.6347938549197849 1106 16-17 0.5818998436078715 1106 18-19 0.5853346885192394 1106 20-21 0.4944208672852213 1106 22-23 0.5854709200527353 1106 24-25 0.5740032407336955 1106 26-27 0.45973560559485804 1106 28-29 0.5671490775610337 1106 30-31 0.6526595485251008 1106 32-33 0.6187119002310766 1106 34-35 0.4888660654457979 1106 36-37 0.4665487136937969 1106 38-39 0.6279496102169659 1106 40-41 0.5549765426031499 1106 42-43 0.4439540807555531 1106 44-45 0.463647820298128 1106 46-47 0.3660499771637262 1106 48-49 0.2859663537792585 1106 50-51 0.1756398039200704 1106 52-53 0.15334694369827417 1106 54-55 0.21902008443896648 1106 56-57 0.17950647833895061 1106 58-59 0.21690622734004705 1106 60-61 0.15999424809216833 1106 62-63 0.10505726377958524 1106 64-65 0.11558682389932073 1106 66-67 0.14039551874920875 1106 68-69 0.16458007682849995 1106 70-71 0.24805144504260923 1106 72-73 0.3068578569429157 1106 74-75 0.4164948721481956 1106 76-77 0.27361086600156526 1106 78-79 0.24281933732433814 1106 80-81 0.15718714888917162 1106 82-83 0.3893424045265661 1106 84-85 0.32702629304463926 1106 86-87 0.3261972174368424 1106 88-89 0.5149577907657203 1106 90-91 0.6623948973696727 1106 92-93 0.6849260538957971 1106 94-95 0.6978409417145031 1106 96-97 1.1148756429715547 1106 98-99 1.1863658687861225 1106 100-101 1.0436459048359659 1107 1 0.24166382821359633 1107 2 0.23003902863749204 1107 3 0.17386059030621936 1107 4 0.20065699749919474 1107 5 0.20526763212597388 1107 6 0.1412465302684538 1107 7 0.1554952097212663 1107 8 0.13802428638886965 1107 9 0.21584663792175718 1107 10-11 0.35186831880224645 1107 12-13 0.26385670694551067 1107 14-15 0.41431523382771474 1107 16-17 0.3382685920103583 1107 18-19 0.37682598385308097 1107 20-21 0.3383549684571179 1107 22-23 0.30627640181075577 1107 24-25 0.3130277191379136 1107 26-27 0.32663231400984216 1107 28-29 0.36766699422447857 1107 30-31 0.4033807849155906 1107 32-33 0.37947287110536365 1107 34-35 0.3027731347758973 1107 36-37 0.43027234344793186 1107 38-39 0.32158073272258747 1107 40-41 0.3879781097786932 1107 42-43 0.40313057776965167 1107 44-45 0.37847557298905343 1107 46-47 0.5188361674296829 1107 48-49 0.695380767146986 1107 50-51 0.5182171493260839 1107 52-53 0.5801539074311961 1107 54-55 0.44958078215481834 1107 56-57 0.46613281669912254 1107 58-59 0.37921165358855546 1107 60-61 0.48084955931981455 1107 62-63 0.4309373477409508 1107 64-65 0.4948495026593136 1107 66-67 0.42124680230987366 1107 68-69 0.6167941318371319 1107 70-71 0.5952265272096007 1107 72-73 0.6090018150551835 1107 74-75 0.5554264043914792 1107 76-77 0.6464540632462281 1107 78-79 0.48250295891986283 1107 80-81 0.557574940927303 1107 82-83 0.6289733566308904 1107 84-85 0.5041295226655507 1107 86-87 0.4809685827901262 1107 88-89 0.6187925944556838 1107 90-91 0.8585084569236301 1107 92-93 0.8874283710952291 1107 94-95 0.9025548611766574 1107 96-97 1.0993673178355543 1107 98-99 1.010777403404699 1107 100-101 1.0623027811736776 1108 1 0.11345218829248438 1108 2 -0.03875767312555922 1108 3 -8.517062422512822E-4 1108 4 0.11353650294667972 1108 5 0.16185472562330006 1108 6 0.10118402483469424 1108 7 0.04782616821417918 1108 8 0.18660006850755906 1108 9 0.21651790784364522 1108 10-11 0.1786394967505487 1108 12-13 0.1779069309309378 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68-69 -0.6664777812103964 2316 70-71 -0.7723147954257179 2316 72-73 -0.4565927177619109 2316 74-75 -0.4714912302277945 2316 76-77 -0.7653854743134403 2316 78-79 -0.5931091058902034 2316 80-81 -0.6581702270843941 2316 82-83 -0.5117749212686924 2316 84-85 -0.6602774217141061 2316 86-87 -0.516973254531635 2316 88-89 -0.6389941760812405 2316 90-91 -0.6694984159286008 2316 92-93 -1.184303525469904 2316 94-95 -0.91034456504903 2316 96-97 -0.9309490355099044 2316 98-99 -1.0367712274671597 2316 100-101 -0.7297644779266292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 15.0 5 34.0 6 89.0 7 236.0 8 540.0 9 1032.0 10 1789.0 11 2952.0 12 4752.0 13 6668.0 14 8367.0 15 11616.0 16 16251.0 17 23719.0 18 20440.0 19 11042.0 20 8619.0 21 8473.0 22 8902.0 23 9496.0 24 10596.0 25 12512.0 26 15033.0 27 18494.0 28 22824.0 29 28646.0 30 35853.0 31 45314.0 32 57783.0 33 75203.0 34 105391.0 35 150154.0 36 228780.0 37 339328.0 38 252413.0 39 20110.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44859527309621 20.12701970673391 15.69834610823031 20.726038911939572 2 15.531231450969155 22.02313639805964 39.72121446261539 22.724417688355814 3 18.714849214202246 27.283757706548943 30.904420564101155 23.096972515147655 4 12.637538703997327 17.997094921613414 37.9063479992248 31.45901837516446 5 12.709049566821987 38.90555791857085 33.5568568129995 14.828535701607667 6 29.386045143175117 38.12046281668341 17.46889930731002 15.024592732831458 7 26.60130785840744 34.94107985304502 21.269200855531793 17.188411433015748 8 26.700446186436345 33.78595843097926 19.919192668624703 19.5944027139597 9 27.422173214486733 16.32539629747127 20.898551429126968 35.35387905891503 10-11 24.478603516090335 27.678538020728897 26.872734006003956 20.970124457176812 12-13 26.381461903602997 25.42534625892611 27.82540535855856 20.367786478912336 14-15 23.34071220974856 25.77260737640329 27.52949844960447 23.357181964243683 16-17 20.931810739175376 29.26796898185577 27.88940256045519 21.910817718513666 18-19 20.801235967129568 26.87748805223247 31.863378429546547 20.457897551091417 20-21 22.13218193773202 25.63577914286226 32.032619179806524 20.19941973959919 22-23 21.740651575467933 25.7535792134429 31.99113895228057 20.5146302588086 24-25 21.5915894210867 25.956241860750715 32.093843465446085 20.358325252716494 26-27 21.197594296207555 25.993427360371385 32.25735888109062 20.551619462330446 28-29 20.938120733121895 26.211675851104964 32.25280929959157 20.597394116181572 30-31 21.235982117754528 25.98932400391114 31.989126335818895 20.78556754251543 32-33 21.09927235176562 26.169944063221255 31.910219727188675 20.820563857824446 34-35 21.518864681191285 26.04152708374975 31.868910653607834 20.57069758145113 36-37 21.572186164976664 25.832043950111583 31.666985414565225 20.928784470346532 38-39 21.261190500023826 26.355260578386506 31.65148162492153 20.732067296668138 40-41 21.368033068687886 26.2621988758565 31.609859841852355 20.759908213603257 42-43 21.295337168683 26.464872139198313 30.89242077638487 21.347369915733815 44-45 21.505097806700686 26.435392123847873 30.710795530316766 21.348714539134672 46-47 21.99261513507152 26.894442355072183 29.999974415849895 21.1129680940064 48-49 21.960574875117526 27.195405092518566 29.359405680953266 21.484614351410645 50-51 21.83770715140041 27.415300581399066 29.244437053477203 21.50255521372332 52-53 22.11514814460661 27.655248262582464 28.746964656173457 21.482638936637468 54-55 21.777617588063123 27.585479051108337 28.824330443948664 21.812572916879873 56-57 21.872116781598315 27.693511625296495 28.556146585901637 21.87822500720355 58-59 22.155681537262517 27.22797497464749 28.8647670910794 21.751576397010595 60-61 22.08003361784202 27.484024969050925 29.231308470244276 21.20463294286278 62-63 21.82156124318184 27.438355148029515 29.40276512786926 21.33731848091939 64-65 21.79636666459861 27.522646784358095 29.088269851554273 21.59271669948902 66-67 21.923943584498847 27.691463416974155 28.68946721749236 21.695125781034633 68-69 21.816572346674583 27.454984803053716 28.996170062527504 21.7322727877442 70-71 22.076048124988606 27.525623284865304 28.728012456902768 21.67031613324332 72-73 21.992618982135888 27.624770542447248 28.49133657825222 21.89127389716464 74-75 21.828922516433032 28.203761256233744 28.17808114913842 21.789235078194807 76-77 21.813383034920207 27.771785032535117 28.39095483140167 22.023877101143004 78-79 21.6986031684711 27.810212815492747 28.411472707770614 22.07971130826554 80-81 21.899771842396635 28.24958279843904 27.968431482039385 21.882213877124947 82-83 22.060642188954617 27.991118357196697 28.020060769471606 21.928178684377077 84-85 21.735118899135617 28.041717356625767 27.915839956608117 22.307323787630498 86-87 21.686715778061693 28.005423893594468 27.961162875111334 22.34669745323251 88-89 21.676234676180627 27.896650786254686 27.804067462062427 22.62304707550226 90-91 21.76603964258988 27.63820220407171 27.996347867244015 22.599410286094393 92-93 21.815612943500106 27.833981037076455 27.948661696531822 22.401744322891616 94-95 21.658803284104177 27.543788002395953 28.077120738766737 22.720287974733132 96-97 21.48249277481372 27.601123025965897 28.183583212687036 22.73280098653335 98-99 21.333672756997498 27.933277665293943 27.71356906861144 23.01948050909712 100-101 21.10630127243259 27.960673172741007 27.842857709365497 23.090167845460908 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1369.0 1 971.0 2 625.5 3 695.0 4 638.5 5 659.0 6 1071.5 7 2204.5 8 3731.5 9 4498.0 10 4062.0 11 3527.0 12 3578.0 13 3821.0 14 4038.5 15 4187.0 16 4317.5 17 4430.5 18 4615.5 19 4827.0 20 5072.0 21 5406.5 22 5977.0 23 6771.5 24 7591.5 25 8804.0 26 10504.0 27 12398.5 28 14152.5 29 16615.5 30 19884.5 31 22916.0 32 26236.0 33 29713.0 34 33389.5 35 36883.0 36 41850.0 37 46348.0 38 47969.5 39 48678.5 40 48137.0 41 47411.0 42 46688.0 43 48331.5 44 51170.0 45 52138.5 46 51930.5 47 53884.5 48 60425.5 49 59769.5 50 53026.5 51 49130.0 52 46930.5 53 46639.5 54 45519.0 55 42608.0 56 39282.0 57 35792.0 58 33492.0 59 30979.0 60 26571.5 61 21920.5 62 17730.0 63 13613.5 64 9978.0 65 7514.5 66 5435.5 67 4084.5 68 2940.0 69 1809.0 70 1232.0 71 868.0 72 643.5 73 471.5 74 339.0 75 271.5 76 191.5 77 91.0 78 46.5 79 28.0 80 19.5 81 11.0 82 6.5 83 4.5 84 5.5 85 6.0 86 3.5 87 2.5 88 1.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015990052907887062 2 0.003070090158314316 3 0.001854846137314899 4 1.9188063489464474E-4 5 0.0017269257140518025 6 1.2792042326309648E-4 7 0.0 8 0.0 9 0.0 10-11 1.5990052907887062E-4 12-13 6.715822221312565E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002302567618735737 22-23 0.0 24-25 0.0038376126978928943 26-27 0.001343164444262513 28-29 0.00310207026413009 30-31 0.015702231955545095 32-33 6.715822221312565E-4 34-35 0.0022066273012884144 36-37 0.00402949333278754 38-39 0.002334547724551511 40-41 0.003805632592077121 42-43 0.002142667089656866 44-45 3.5178116397351533E-4 46-47 2.5584084652619296E-4 48-49 1.2792042326309648E-4 50-51 1.2792042326309648E-4 52-53 0.0010553434919205462 54-55 0.003070090158314316 56-57 4.157413756050636E-4 58-59 0.0011193037035520945 60-61 0.0010553434919205462 62-63 0.0 64-65 3.1980105815774125E-4 66-67 6.396021163154824E-5 68-69 0.0 70-71 9.594031744732237E-5 72-73 1.2792042326309648E-4 74-75 3.1980105815774125E-4 76-77 4.797015872366118E-4 78-79 3.1980105815774125E-4 80-81 0.005052856718892312 82-83 0.0014391047617098357 84-85 0.0029421697350512192 86-87 0.0012472241268151908 88-89 9.91383280288998E-4 90-91 1.2792042326309648E-4 92-93 0.0 94-95 9.594031744732237E-5 96-97 0.0011193037035520945 98-99 0.0023985079361830593 100-101 6.396021163154825E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1563472.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.72734345971439 #Duplication Level Percentage of deduplicated Percentage of total 1 69.44624421304827 30.36699772691164 2 15.119811525205936 13.22298383217656 3 6.014114924306938 7.88943806704091 4 2.904664371837523 5.080530264901396 5 1.6609297866554122 3.6314023621775675 6 1.0781793799738213 2.8287552035578316 7 0.6904001998602446 2.113255666476106 8 0.4957764715721632 1.7343190441345044 9 0.36010150820270176 1.4171654096586628 >10 1.9726746137015612 15.818342827119164 >50 0.16813906722370234 5.076785240753738 >100 0.08352323258203247 6.475767051852041 >500 0.0033820603806450334 1.0444787181420077 >1k 0.001617507138569364 1.1923672340079654 >5k 1.4704610350630578E-4 0.4851386313950658 >10k+ 2.9409220701261156E-4 1.622272719694832 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14196 0.907979164321459 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11034 0.7057369751425033 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7545 0.48257979676003154 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3917 0.2505321489607745 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2376 0.15196946283655863 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1857 0.1187741129997851 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1682 0.10758107596426415 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1595 0.10201653755231946 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.396021163154824E-5 0.0 0.0 0.0 0.0 7 6.396021163154824E-5 0.0 0.0 0.0 0.0 8 6.396021163154824E-5 0.0 0.0 0.0 0.0 9 6.396021163154824E-5 0.0 0.0 0.0 0.0 10-11 6.396021163154824E-5 0.0 0.0 0.0 0.0 12-13 6.396021163154824E-5 0.0 0.0 0.0 0.0 14-15 1.2792042326309648E-4 0.0 0.0 0.0 0.0 16-17 1.2792042326309648E-4 0.0 0.0 1.599005290788706E-4 0.0 18-19 1.2792042326309648E-4 0.0 0.0 2.2386074071041885E-4 0.0 20-21 1.2792042326309648E-4 0.0 0.0 4.157413756050636E-4 0.0 22-23 1.2792042326309648E-4 0.0 0.0 6.396021163154824E-4 0.0 24-25 1.2792042326309648E-4 0.0 0.0 8.634628570259014E-4 0.0 26-27 1.2792042326309648E-4 0.0 0.0 0.0013751445500782872 0.0 28-29 1.2792042326309648E-4 0.0 0.0 0.0022705875129199627 0.0 30-31 1.2792042326309648E-4 0.0 0.0 0.004541175025839925 0.0 32-33 1.2792042326309648E-4 0.0 0.0 0.007163543702733404 0.0 34-35 1.2792042326309648E-4 0.0 0.0 0.01055343491920546 0.0 36-37 1.2792042326309648E-4 0.0 0.0 0.015510351320650451 0.0 38-39 1.2792042326309648E-4 0.0 0.0 0.021842412272173727 0.0 40-41 1.2792042326309648E-4 0.0 0.0 0.030349120419169644 0.0 42-43 1.2792042326309648E-4 0.0 0.0 0.03738474369863995 0.0 44-45 1.2792042326309648E-4 0.0 0.0 0.042437600417532265 0.0 46-47 1.2792042326309648E-4 0.0 0.0 0.05084836824708086 0.0 48-49 1.2792042326309648E-4 0.0 0.0 0.057660130785840744 0.0 50-51 1.599005290788706E-4 0.0 0.0 0.06408813205481134 0.0 52-53 1.9188063489464474E-4 0.0 0.0 0.07911878178822518 0.0 54-55 1.9188063489464474E-4 0.0 0.0 0.10671761310723825 0.0 56-57 1.9188063489464474E-4 0.0 0.0 0.1500186763817964 0.0 58-59 1.9188063489464474E-4 0.0 0.0 0.20643158304082196 0.0 60-61 1.9188063489464474E-4 0.0 0.0 0.2735258450423161 0.0 62-63 3.1980105815774125E-4 0.0 0.0 0.31244563382011314 0.0 64-65 3.1980105815774125E-4 0.0 0.0 0.357921344290144 6.396021163154824E-5 66-67 4.157413756050636E-4 0.0 0.0 0.45133523337802023 6.396021163154824E-5 68-69 4.477214814208377E-4 0.0 0.0 0.5757058648955657 6.396021163154824E-5 70-71 4.797015872366118E-4 0.0 0.0 0.7096705281578436 6.396021163154824E-5 72-73 5.756419046839342E-4 0.0 0.0 0.8575465374499831 6.396021163154824E-5 74-75 5.756419046839342E-4 0.0 0.0 0.9924066436751027 6.396021163154824E-5 76-77 5.756419046839342E-4 0.0 0.0 1.1146665882088072 6.396021163154824E-5 78-79 5.756419046839342E-4 0.0 0.0 1.2261812171884114 6.396021163154824E-5 80-81 5.756419046839342E-4 0.0 0.0 1.3375039655331211 6.396021163154824E-5 82-83 5.756419046839342E-4 0.0 0.0 1.4975963752468866 6.396021163154824E-5 84-85 5.756419046839342E-4 0.0 0.0 1.7802365504466982 6.396021163154824E-5 86-87 5.756419046839342E-4 0.0 0.0 2.1480397474339163 6.396021163154824E-5 88-89 5.756419046839342E-4 0.0 0.0 2.570049223778872 6.396021163154824E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 15455 0.0 31.476805 1 GGTATCA 10335 0.0 31.349716 1 GTACATG 9840 0.0 26.408998 1 ATCAACG 19000 0.0 25.548986 3 TCAACGC 19135 0.0 25.368732 4 TATCAAC 19180 0.0 25.310022 2 CAACGCA 19320 0.0 25.125813 5 AACGCAG 19675 0.0 24.672462 6 TACATGG 10290 0.0 24.51147 2 ACATGGG 9945 0.0 24.166964 3 GAGTACT 11050 0.0 24.135532 12-13 GTACTTT 11570 0.0 23.072422 14-15 AGTACTT 11535 0.0 22.585434 12-13 ACGCAGA 21610 0.0 22.265425 7 CGCAGAG 21665 0.0 22.143133 8 CATGGGA 6495 0.0 21.865952 4 AGAGTAC 16670 0.0 21.811522 10-11 CCTATAC 425 0.0 21.23445 3 GCAGAGT 22130 0.0 21.227129 9 TACTTTT 12455 0.0 20.613047 14-15 >>END_MODULE