FastQCFastQC Report
Thu 26 May 2016
SRR937046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416613
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121310.8563383224635098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85740.6052464575716868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56260.3971444565311768No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28510.20125468282445524No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20330.14351131889937477No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18490.13052259156170387No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15770.11132186419297296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83450.034.1199341
GTATCAA130050.034.065381
TCAACGC157800.027.9824494
ATCAACG157950.027.9558773
CAACGCA159100.027.7567485
TATCAAC159450.027.635222
AACGCAG161950.027.2615436
GTACATG93900.025.0580541
ACCGTCC1151.2775166E-424.7952828
GAGTACT85200.024.56704512-13
ACGCAGA178150.024.4624067
CGCAGAG177800.024.2699998
TACATGG96950.024.024592
GCAGAGT181950.023.2462889
ACATGGG95400.023.1178073
GTACTTT91250.022.88856914-15
AGTACTT89550.021.86141412-13
AGAGTAC141500.021.69468510-11
CATGGGA61800.021.6119964
ATGGGAT26800.021.2847985
Produced by FastQC (version 0.11.5)