##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937046_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1416613 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.963104249360974 33.0 31.0 34.0 30.0 34.0 2 32.06765644533828 33.0 31.0 34.0 30.0 34.0 3 32.06980876216723 34.0 31.0 34.0 30.0 34.0 4 35.67033833516987 37.0 35.0 37.0 33.0 37.0 5 35.68324941250716 37.0 35.0 37.0 33.0 37.0 6 35.77197936204171 37.0 35.0 37.0 35.0 37.0 7 35.71562805085087 37.0 35.0 37.0 33.0 37.0 8 35.669170761527674 37.0 35.0 37.0 33.0 37.0 9 37.329771786648855 39.0 37.0 39.0 34.0 39.0 10-11 37.15474550918282 39.0 37.0 39.0 33.5 39.0 12-13 37.09411356524329 39.0 37.0 39.0 33.0 39.0 14-15 38.24510752054372 40.0 38.0 41.0 33.0 41.0 16-17 38.19980545145357 40.0 38.0 41.0 33.0 41.0 18-19 38.22251772361259 40.0 38.0 41.0 33.5 41.0 20-21 38.176521392928066 40.0 38.0 41.0 33.5 41.0 22-23 38.07569780878758 40.0 38.0 41.0 33.0 41.0 24-25 37.9129762327467 40.0 38.0 41.0 33.0 41.0 26-27 37.987347991300375 40.0 38.0 41.0 33.0 41.0 28-29 37.93778858446167 40.0 38.0 41.0 33.0 41.0 30-31 37.749154497382136 40.0 37.0 41.0 33.0 41.0 32-33 37.50110686546007 40.0 36.5 41.0 33.0 41.0 34-35 37.316038678171104 40.0 36.0 41.0 32.0 41.0 36-37 37.121033761514255 40.0 36.0 41.0 31.5 41.0 38-39 36.94852899133355 39.0 36.0 41.0 31.0 41.0 40-41 36.7523833255801 39.0 35.0 41.0 31.0 41.0 42-43 36.49096365768209 39.0 35.0 41.0 30.0 41.0 44-45 36.147367347327744 39.0 35.0 41.0 30.0 41.0 46-47 35.660277718755935 39.0 35.0 40.5 28.0 41.0 48-49 35.23698074209399 39.0 35.0 40.0 26.0 41.0 50-51 34.51985298737199 38.0 34.0 39.5 25.0 40.5 52-53 34.72593044112965 38.5 34.5 40.0 26.0 40.5 54-55 35.09364872410461 39.0 35.0 40.5 26.0 41.0 56-57 34.97476022032835 39.0 35.0 41.0 26.0 41.0 58-59 34.733434254803534 38.0 35.0 40.5 25.0 41.0 60-61 34.447799081329904 38.0 34.0 40.0 24.0 41.0 62-63 34.133615532258986 37.5 34.0 40.0 23.0 41.0 64-65 33.81917538523224 37.0 34.0 40.0 22.5 41.0 66-67 33.440712459930836 36.5 33.5 39.5 22.0 41.0 68-69 33.05756088642417 36.0 33.0 39.0 20.5 41.0 70-71 32.64038696524739 35.5 33.0 39.0 19.5 40.5 72-73 32.25592734218873 35.0 32.5 38.0 18.0 40.0 74-75 31.83461185235488 35.0 32.0 37.0 16.0 39.5 76-77 31.426610160996688 35.0 32.0 37.0 12.0 39.0 78-79 31.002577627058344 35.0 31.0 36.0 7.5 39.0 80-81 30.591867009550242 35.0 31.0 36.0 6.5 37.5 82-83 30.201780938054362 35.0 31.0 35.5 2.0 37.0 84-85 29.86003693316382 34.0 30.5 35.0 2.0 37.0 86-87 29.563461933499127 34.0 30.0 35.0 2.0 36.0 88-89 29.231801134113553 34.0 30.0 35.0 2.0 36.0 90-91 28.900283634274146 34.0 29.0 35.0 2.0 36.0 92-93 28.565065758961694 34.0 29.0 35.0 2.0 35.0 94-95 28.176858817475203 34.0 29.0 35.0 2.0 35.0 96-97 27.76231652540249 34.0 29.0 35.0 2.0 35.0 98-99 27.229231272055245 34.0 27.0 35.0 2.0 35.0 100-101 25.077231043340703 31.5 22.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1354484908295568 1101 2 0.31554215393018836 1101 3 0.1328699067935588 1101 4 0.07462222786529793 1101 5 0.0700849471636289 1101 6 -0.0042080822570582654 1101 7 0.07913054242779793 1101 8 0.03484849688918956 1101 9 -0.015097134639773913 1101 10-11 0.07217763064419813 1101 12-13 0.13144360330275617 1101 14-15 0.395983885980165 1101 16-17 0.33646248878136475 1101 18-19 0.2906109033161215 1101 20-21 0.3567114292785831 1101 22-23 0.321764016157303 1101 24-25 0.4152161169007087 1101 26-27 0.5012153693297847 1101 28-29 0.5593724876243371 1101 30-31 0.4164368966955223 1101 32-33 0.4323027257858101 1101 34-35 0.43631118449381034 1101 36-37 0.3627287735368725 1101 38-39 0.5621969836104412 1101 40-41 0.534426069480979 1101 42-43 0.48988769573754354 1101 44-45 0.6743681537245649 1101 46-47 0.6091937130882172 1101 48-49 0.7193748063720875 1101 50-51 0.6224128510521112 1101 52-53 0.6580697435925558 1101 54-55 0.6608560528144238 1101 56-57 0.6543841335289642 1101 58-59 0.6159698408693117 1101 60-61 0.7688246812463007 1101 62-63 0.6358724673203113 1101 64-65 0.7330361267401457 1101 66-67 0.7658482173157566 1101 68-69 0.8888619117703556 1101 70-71 0.9015773611999904 1101 72-73 0.8489280625076177 1101 74-75 0.7881215059681175 1101 76-77 0.9051299886444575 1101 78-79 0.7565995969725314 1101 80-81 0.5237327264196914 1101 82-83 0.6977774099441838 1101 84-85 0.5864359145582156 1101 86-87 0.8204322157817607 1101 88-89 0.7254008306802007 1101 90-91 0.9385484775947575 1101 92-93 0.9453231697859614 1101 94-95 1.0005894493528444 1101 96-97 0.930710501222098 1101 98-99 0.8747828854762787 1101 100-101 1.1353455763329343 1102 1 0.2510951055239907 1102 2 0.17115631830206013 1102 3 0.2161360123669951 1102 4 0.1997111107209264 1102 5 0.11283663108972775 1102 6 0.17213033194351368 1102 7 0.174524794454328 1102 8 0.2790261850299558 1102 9 0.2561468374865612 1102 10-11 0.4370595295698436 1102 12-13 0.32771925004674074 1102 14-15 0.23449246398375578 1102 16-17 0.3789976217501163 1102 18-19 0.3861897084719672 1102 20-21 0.19278526345873104 1102 22-23 0.26328491494073347 1102 24-25 0.3953483745898865 1102 26-27 0.3615277198449718 1102 28-29 0.3100050103460248 1102 30-31 0.287961092224883 1102 32-33 0.3985120497589705 1102 34-35 0.4028905843567614 1102 36-37 0.3754584145147817 1102 38-39 0.5591927430902004 1102 40-41 0.2783981958174522 1102 42-43 0.3356151776700784 1102 44-45 0.42202875696442277 1102 46-47 0.4412413155135937 1102 48-49 0.6199029321397092 1102 50-51 0.5810050759031142 1102 52-53 0.5298066115546547 1102 54-55 0.4981516222464748 1102 56-57 0.6311016735100878 1102 58-59 0.6934766606084182 1102 60-61 0.6563422962122445 1102 62-63 0.6491295876450778 1102 64-65 0.7453074553933874 1102 66-67 0.5845381809869039 1102 68-69 0.5949369741499524 1102 70-71 0.6267593286462372 1102 72-73 0.79347388727588 1102 74-75 0.6695375553029486 1102 76-77 0.7147920398668397 1102 78-79 0.7047033216489993 1102 80-81 0.7316620467728931 1102 82-83 0.6973730737536705 1102 84-85 0.6480957291647371 1102 86-87 0.5127857045146449 1102 88-89 0.5215630305793582 1102 90-91 0.54879909492659 1102 92-93 0.6269289436162637 1102 94-95 0.5998289353190458 1102 96-97 0.5706993827848841 1102 98-99 0.7678839598275964 1102 100-101 0.8809709237840941 1103 1 0.3226280317289003 1103 2 0.23798459175885256 1103 3 0.3117515560361852 1103 4 0.2786231217637436 1103 5 0.22990491042998684 1103 6 0.1281217104004071 1103 7 0.25442751923532825 1103 8 0.26186937081459405 1103 9 0.2761838637330527 1103 10-11 0.36340869640858386 1103 12-13 0.42357345601902807 1103 14-15 0.36427737798047133 1103 16-17 0.45258269018786734 1103 18-19 0.2510251693766108 1103 20-21 0.5069663300069749 1103 22-23 0.5257851624423822 1103 24-25 0.4895284087026823 1103 26-27 0.5245761481387987 1103 28-29 0.4850399434357229 1103 30-31 0.415298922073859 1103 32-33 0.7182604890842939 1103 34-35 0.6242334292962752 1103 36-37 0.6391703593685776 1103 38-39 0.7131348786192788 1103 40-41 0.7761784596117778 1103 42-43 0.8470741503826673 1103 44-45 0.7644465793459219 1103 46-47 0.9252887736009541 1103 48-49 0.7320650216759077 1103 50-51 0.7989529053843754 1103 52-53 0.901215009073411 1103 54-55 0.9561162959827314 1103 56-57 1.1046016720957894 1103 58-59 1.032709136784618 1103 60-61 1.0882673215988348 1103 62-63 1.2309214571452358 1103 64-65 1.0365939123860954 1103 66-67 0.9677711465092145 1103 68-69 1.0345614222212802 1103 70-71 0.9404004880288213 1103 72-73 1.0277416218296551 1103 74-75 0.7172629444462579 1103 76-77 0.9968809820589435 1103 78-79 0.8990978336181747 1103 80-81 0.8973979893864552 1103 82-83 0.9418095902881944 1103 84-85 0.8102747195277331 1103 86-87 0.9282688418738161 1103 88-89 0.9346128731683123 1103 90-91 0.9972109122109778 1103 92-93 1.0044569961601546 1103 94-95 0.9431260956972842 1103 96-97 1.0642784828971834 1103 98-99 1.0687480234369353 1103 100-101 1.1950621825916201 1104 1 0.37943744522798895 1104 2 0.26166138643009873 1104 3 0.2746266651094871 1104 4 0.1521414288283225 1104 5 0.240197979973523 1104 6 0.242809182264331 1104 7 0.27371235179887066 1104 8 0.23803564522408038 1104 9 0.30186163280646383 1104 10-11 0.4831368903735225 1104 12-13 0.3908977459802401 1104 14-15 0.37893800750485696 1104 16-17 0.6034120259198943 1104 18-19 0.4467188671486966 1104 20-21 0.4115748399541985 1104 22-23 0.4149887779961361 1104 24-25 0.5009772134612618 1104 26-27 0.3304319854220523 1104 28-29 0.2962312216107108 1104 30-31 0.4588811206381749 1104 32-33 0.5422910725223105 1104 34-35 0.41136589329682494 1104 36-37 0.42065856935477086 1104 38-39 0.45423315103284523 1104 40-41 0.5027238084401873 1104 42-43 0.5875831955779276 1104 44-45 0.6835930770937821 1104 46-47 0.7781144978182084 1104 48-49 0.7774561330749847 1104 50-51 0.6159422130455567 1104 52-53 0.5902162131921571 1104 54-55 0.7723848994490154 1104 56-57 0.5537430117388951 1104 58-59 0.7498598771367284 1104 60-61 0.8074600571698198 1104 62-63 0.832224572791965 1104 64-65 0.852382467115433 1104 66-67 0.6487644561192027 1104 68-69 0.7546172778482045 1104 70-71 0.6191509055996889 1104 72-73 0.561267830134625 1104 74-75 0.642422252469256 1104 76-77 0.8381106473038322 1104 78-79 0.9053210070558713 1104 80-81 0.8899557614217848 1104 82-83 0.7257729332449436 1104 84-85 0.7951799004358442 1104 86-87 0.7496172949893385 1104 88-89 0.9175429543432507 1104 90-91 0.9847809736622644 1104 92-93 1.0749572650042793 1104 94-95 1.1317286616229971 1104 96-97 1.1705263950427955 1104 98-99 1.3141616857670328 1104 100-101 1.5337237343481078 1105 1 0.1492933103156595 1105 2 0.21257394413171937 1105 3 0.3660333872122834 1105 4 0.3161386168646061 1105 5 0.33379785959903074 1105 6 0.3386864041486888 1105 7 0.30149089562549136 1105 8 0.2246657544271713 1105 9 0.15172492937429638 1105 10-11 0.33989172361759046 1105 12-13 0.40612889355850257 1105 14-15 0.47879440963308184 1105 16-17 0.3613724426440186 1105 18-19 0.19603655953863353 1105 20-21 0.37752985530823935 1105 22-23 0.37264941964831877 1105 24-25 0.46530465724980985 1105 26-27 0.37053222331650204 1105 28-29 0.29053326967051873 1105 30-31 0.03655121613946477 1105 32-33 0.0777606661084107 1105 34-35 0.08100418438539236 1105 36-37 -0.09541031311220394 1105 38-39 0.02744339079967517 1105 40-41 -0.08096549569555833 1105 42-43 0.13787776958139375 1105 44-45 0.17545352589518615 1105 46-47 0.08603589443330861 1105 48-49 0.3245860383478032 1105 50-51 0.3309892186090053 1105 52-53 0.25358128997685725 1105 54-55 0.35970382364549636 1105 56-57 0.5941405572216425 1105 58-59 0.42865761266409663 1105 60-61 0.361776387605552 1105 62-63 0.47497116270537276 1105 64-65 0.4346563548971858 1105 66-67 0.4251630234304571 1105 68-69 0.7029109161431606 1105 70-71 0.588107784145798 1105 72-73 0.49309343419638907 1105 74-75 0.5179023836619727 1105 76-77 0.5545776655143442 1105 78-79 0.6062496517832265 1105 80-81 0.6648823054012958 1105 82-83 0.60793244590241 1105 84-85 0.5149308490865394 1105 86-87 0.6444260480730861 1105 88-89 0.470717226907297 1105 90-91 0.35528501283622305 1105 92-93 0.44203513056318755 1105 94-95 0.556392757268469 1105 96-97 0.569878462866658 1105 98-99 0.6083089597056173 1105 100-101 0.7142910427056712 1106 1 0.2252996187111158 1106 2 0.23204592728166773 1106 3 0.14214863993719007 1106 4 0.22193964301643376 1106 5 0.1446694661128589 1106 6 0.11801551459229387 1106 7 0.25410270622836606 1106 8 0.2988511361732904 1106 9 0.3949156493925585 1106 10-11 0.4222580463314429 1106 12-13 0.4015475251139691 1106 14-15 0.49884379772910137 1106 16-17 0.4857181281891769 1106 18-19 0.4168119837730444 1106 20-21 0.4431356117191001 1106 22-23 0.27410019496880267 1106 24-25 0.4157295401346559 1106 26-27 0.23430745560138178 1106 28-29 0.3177089138687137 1106 30-31 0.3731959383054928 1106 32-33 0.4151679985805643 1106 34-35 0.29395273672276545 1106 36-37 0.21271433995198663 1106 38-39 0.23629900843620533 1106 40-41 0.2829765251698788 1106 42-43 0.4236519746768792 1106 44-45 0.3551343708881589 1106 46-47 0.4814791681585291 1106 48-49 0.5196490444849999 1106 50-51 0.4300997659764363 1106 52-53 0.42424154449155793 1106 54-55 0.5871354870178962 1106 56-57 0.41224425147196797 1106 58-59 0.5282713790770686 1106 60-61 0.6166122188767247 1106 62-63 0.5391365194461741 1106 64-65 0.5046390794392295 1106 66-67 0.6715556691694431 1106 68-69 0.5713663449428523 1106 70-71 0.48964078649588316 1106 72-73 0.6018292130705305 1106 74-75 0.7181846013534958 1106 76-77 0.8148747203859763 1106 78-79 0.6098883923867753 1106 80-81 0.46245803467982327 1106 82-83 0.586605815239249 1106 84-85 0.6372833721853333 1106 86-87 0.7295748608392856 1106 88-89 0.7568742935034116 1106 90-91 0.9266119028906452 1106 92-93 0.8124352156435215 1106 94-95 0.7735903978455596 1106 96-97 0.9242813701239108 1106 98-99 1.0169949354577206 1106 100-101 1.1248028735410642 1107 1 0.17646100572588708 1107 2 0.05856473300445941 1107 3 0.2498463775215285 1107 4 0.18371284901748908 1107 5 0.15336107067349758 1107 6 0.09514736692914028 1107 7 0.08524506501281337 1107 8 0.09419586939442581 1107 9 0.13222786070821257 1107 10-11 0.17438030782638236 1107 12-13 0.2970743564532654 1107 14-15 0.2982918767474132 1107 16-17 0.20280747391473142 1107 18-19 0.22748134781071627 1107 20-21 0.3125078335718996 1107 22-23 0.13862507744901365 1107 24-25 0.2924861987364906 1107 26-27 0.12475216375845122 1107 28-29 0.2469318254948618 1107 30-31 0.3305138785507111 1107 32-33 0.42885707115893723 1107 34-35 0.40162975808860324 1107 36-37 0.4213302408005859 1107 38-39 0.17107974900265788 1107 40-41 0.4119792310660628 1107 42-43 0.28265867036329695 1107 44-45 0.4527456118663693 1107 46-47 0.4168253847632144 1107 48-49 0.37160915681293716 1107 50-51 0.35579785138864395 1107 52-53 0.3940557123389681 1107 54-55 0.4233673859022886 1107 56-57 0.5500385624403137 1107 58-59 0.5757168903073122 1107 60-61 0.5380675715895222 1107 62-63 0.49281940273458247 1107 64-65 0.5541467495551871 1107 66-67 0.3757746165809337 1107 68-69 0.4666057067810172 1107 70-71 0.3149207112013741 1107 72-73 0.47115437585908637 1107 74-75 0.5219984316032686 1107 76-77 0.40727110093012087 1107 78-79 0.5054484719779744 1107 80-81 0.4839778378501727 1107 82-83 0.3624609347027743 1107 84-85 0.4115300435238076 1107 86-87 0.39981767538053603 1107 88-89 0.5318508918491816 1107 90-91 0.6317797042604774 1107 92-93 0.6495113643706247 1107 94-95 0.621692945684682 1107 96-97 0.6184468134254111 1107 98-99 0.6505920382562032 1107 100-101 0.6140235345163134 1108 1 0.19320053190082476 1108 2 0.22572857042961658 1108 3 0.10512020298060065 1108 4 0.19416493118662004 1108 5 0.1405887472232692 1108 6 0.14227595588054953 1108 7 0.19389153761627398 1108 8 0.25501178259081314 1108 9 0.2326490229881486 1108 10-11 0.22009585884119787 1108 12-13 0.31342575954445095 1108 14-15 0.40685737582920467 1108 16-17 0.4154509395014827 1108 18-19 0.3969331870592825 1108 20-21 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-1.8974357680325014 2316 72-73 -2.079136848250542 2316 74-75 -2.4122998702486953 2316 76-77 -2.6102336879588783 2316 78-79 -2.878249505729645 2316 80-81 -2.9414793383934814 2316 82-83 -2.606182271775616 2316 84-85 -2.7528770226326813 2316 86-87 -3.251132731572355 2316 88-89 -3.210285970729263 2316 90-91 -2.8396750156105917 2316 92-93 -2.9373085669183823 2316 94-95 -3.317042130511716 2316 96-97 -3.333832031984368 2316 98-99 -3.2201511895349633 2316 100-101 -3.214379256981232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2166.0 3 742.0 4 726.0 5 741.0 6 905.0 7 1150.0 8 1353.0 9 1723.0 10 2256.0 11 3222.0 12 4527.0 13 6330.0 14 7271.0 15 9498.0 16 13214.0 17 18939.0 18 15797.0 19 8729.0 20 7872.0 21 7979.0 22 8288.0 23 9222.0 24 10223.0 25 11551.0 26 13238.0 27 15805.0 28 19438.0 29 23058.0 30 28515.0 31 35990.0 32 46652.0 33 61213.0 34 87355.0 35 127971.0 36 195571.0 37 290461.0 38 267408.0 39 49505.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.34461324650067 19.291384456981607 15.124744272299772 21.239258024217953 2 15.133257490856886 21.083572900932353 40.734683385907566 23.048486222303197 3 18.362317919605726 27.197003396245066 30.762142811751996 23.678535872397212 4 12.120225564175268 17.342099780721114 38.73263159381499 31.805043061288625 5 12.714337781369107 38.27340304402736 34.03397195561947 14.978287218984063 6 29.056556860595546 37.58755336552453 18.292614013624277 15.063275760255646 7 26.57244510118729 34.56267777792689 21.724241934082574 17.140635186803234 8 26.17062260148716 33.526521115389265 20.559135592394266 19.743720690729315 9 27.2529366475246 16.295691975164754 21.21739314699837 35.23397823031228 10-11 24.223173594807346 27.23850508842267 27.458762637170896 21.079558679599085 12-13 26.085807872137977 25.122364198520682 28.12430464853272 20.667523280808616 14-15 23.13659633155509 25.501395930346522 27.713199444100095 23.648808293998293 16-17 20.8527751282514 29.13085686095101 27.937033833931963 22.079334176865625 18-19 20.799575412934228 26.975862380682 31.581841063755856 20.64272114262792 20-21 22.263402286299893 25.684235506334097 31.504302836033148 20.54805937133286 22-23 21.814423992159277 25.70767040720352 31.576173072088892 20.901732528548308 24-25 21.561389995191966 26.063680695633018 31.55536053556287 20.819568773612147 26-27 21.11663791481474 26.073746646941025 31.779286029212162 21.03032940903207 28-29 20.929198150923213 26.26656288114263 31.766842643141946 21.03739632479221 30-31 21.225370418874967 26.03214968261914 31.517956675799912 21.224523222705987 32-33 20.993271097842115 26.27526338659013 31.499543064373743 21.231922451194016 34-35 21.485647278258295 26.197277958253366 31.35724035783232 20.959834405656018 36-37 21.472307318156194 25.929863537065884 31.290677944863482 21.30715119991444 38-39 21.281584803149162 26.36208581126492 31.225608964041047 21.130720421544876 40-41 21.275810923097232 26.29390830799511 31.252528233261774 21.177752535645883 42-43 21.174816180169024 26.461690185886493 30.596705414932828 21.766788219011655 44-45 21.333279683352163 26.45830002269535 30.480283808400387 21.728136485552096 46-47 21.698355288353103 26.76915832992428 29.994730230510942 21.537756151211678 48-49 21.73961076480033 27.050678085569057 29.425768839935944 21.783942309694666 50-51 21.55682007103987 27.314911066032206 29.298775139363592 21.829493723564333 52-53 21.784880386152857 27.484129435330868 28.801461309707648 21.929528868808625 54-55 21.545979015795336 27.416000350160324 28.943786560338413 22.094234073705923 56-57 21.67738751406372 27.580566982849586 28.570208105998535 22.17183739708816 58-59 21.9769444026745 27.08443200053089 28.901565772836925 22.03705782395769 60-61 21.863528883472696 27.267146263332886 29.324295414412894 21.545029438781526 62-63 21.616535514233792 27.28890765296685 29.525959144828718 21.56859768797064 64-65 21.545267172312084 27.357271420891387 29.274144556887226 21.8233168499093 66-67 21.57386586274212 27.52249564913495 28.74906893252894 22.154569555593994 68-69 21.44981710561202 27.438500636714657 29.167603703995294 21.94407855367803 70-71 21.76685206333965 27.33249431189357 28.92195158975735 21.978702035009434 72-73 21.62438279264551 27.49136077387132 28.72406334691157 22.160193086571596 74-75 21.469582131598365 28.022176643567466 28.476300080135275 22.031941144698894 76-77 21.410672273891667 27.613841448087513 28.6986996811338 22.27678659688703 78-79 21.389896062507262 27.624097736622243 28.680420721401124 22.30558547946937 80-81 21.519525427223535 28.04172353918537 28.2921509215996 22.146600111991493 82-83 21.64259254813294 27.76112109884182 28.439882726937636 22.156403626087606 84-85 21.447750803486198 27.719673691404818 28.23304347580504 22.599532029303944 86-87 21.444493398913494 27.792840691331243 28.3115580089276 22.45110790082766 88-89 21.545716390727833 27.571748862301703 28.14463127490342 22.73790347206704 90-91 21.388393096689835 27.365528917707593 28.483497180103416 22.76258080549915 92-93 21.56050564122408 27.507834445007774 28.477413213838332 22.45424669992981 94-95 21.399062909077042 27.212987662201034 28.63334552474126 22.754603903980662 96-97 21.12858367049916 27.351619084474294 28.684511298159336 22.83528594686721 98-99 21.00011018007379 27.662331975387467 28.251124401778704 23.08643344276004 100-101 21.000919314684985 27.465011829598684 28.48205005408876 23.05201880162757 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 737.0 1 535.5 2 426.5 3 566.5 4 534.5 5 544.0 6 907.0 7 1891.0 8 3313.5 9 4089.0 10 3578.0 11 2992.0 12 3115.5 13 3279.5 14 3369.0 15 3481.0 16 3540.0 17 3615.5 18 3805.0 19 3983.5 20 4202.0 21 4569.0 22 5070.5 23 5850.0 24 6686.0 25 7851.5 26 9386.5 27 10987.0 28 12685.5 29 14848.5 30 17835.5 31 20995.5 32 24214.0 33 27487.0 34 30756.0 35 33769.5 36 38606.0 37 42919.5 38 44160.0 39 44329.0 40 43671.5 41 43566.5 42 43115.5 43 44188.5 44 46756.5 45 47820.0 46 47536.5 47 48971.5 48 54682.5 49 54521.0 50 48432.0 51 44461.0 52 42492.5 53 42499.5 54 41683.5 55 38689.0 56 35561.5 57 32399.5 58 29942.5 59 27535.0 60 23649.0 61 19724.0 62 15998.5 63 12128.0 64 8843.5 65 6624.0 66 4747.5 67 3522.5 68 2466.0 69 1551.0 70 1080.0 71 736.5 72 523.5 73 384.5 74 288.0 75 238.0 76 166.0 77 85.5 78 49.5 79 27.0 80 25.0 81 20.5 82 13.0 83 10.5 84 8.0 85 6.0 86 5.5 87 6.0 88 3.0 89 1.0 90 2.5 91 3.5 92 2.5 93 1.0 94 1.0 95 1.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03924854565078818 2 0.036707272910809094 3 0.024212681939245227 4 0.04291927294186909 5 0.06049640939339114 6 0.06388477304669658 7 0.06155527303504909 8 0.06296709122392637 9 0.062331773038931594 10-11 0.07041443217025398 12-13 0.06790845488499682 14-15 0.06286120485976057 16-17 0.05234315935262489 18-19 0.0458135002290675 20-21 0.05255493208095648 22-23 0.029436409238091137 24-25 0.016518272809864093 26-27 0.012882840973505115 28-29 0.01627120462681057 30-31 0.01242400006212 32-33 0.007870886402990796 34-35 0.009847431867418977 36-37 0.005259022753567841 38-39 0.007694409129381136 40-41 0.007765000038825 42-43 0.005400204572455568 44-45 0.0016588863719307957 46-47 0.0030001136513642043 48-49 0.0010235681869360227 50-51 0.005964931848006477 52-53 0.005329613663011704 54-55 0.008400318223819774 56-57 0.02057725010288625 58-59 0.008753272771039091 60-61 0.020047818282057272 62-63 0.01376522734155341 64-65 0.009847431867418977 66-67 0.01609472735320091 68-69 0.025306841035625117 70-71 0.019800750099003752 72-73 0.01789479554401943 74-75 0.018212454636516818 76-77 0.024353863758132956 78-79 0.03681315927497489 80-81 0.03063645469863682 82-83 0.030424681970305227 84-85 0.020118409191501135 86-87 0.023365591025918866 88-89 0.01881247736678966 90-91 0.017577136451522046 92-93 0.030424681970305227 94-95 0.022236136474817047 96-97 0.03667197745608716 98-99 0.052872591173453866 100-101 0.06243765940309739 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1416613.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.70294579139103 #Duplication Level Percentage of deduplicated Percentage of total 1 71.5195935772177 33.40175701859117 2 14.56996805733422 13.609208567279579 3 5.485549005506467 7.685738935205626 4 2.667934815265757 4.98401660209286 5 1.500659465916696 3.5042608844022634 6 0.9092904488741126 2.547992552543838 7 0.6089713616107841 1.9908529542872626 8 0.43636620877128085 1.6303669914751961 9 0.3224863294658119 1.3554955407155833 >10 1.7567084579559096 15.204674268407508 >50 0.14780428964785358 4.703996377680711 >100 0.07026684562875324 5.742269233339411 >500 0.0027317400856255404 0.8613088425612307 >1k 0.0012141067047224626 0.9117739426191305 >5k 3.0352667618061566E-4 1.0064668831772117 >10k+ 1.5176333809030783E-4 0.8598204056213208 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12131 0.8563383224635098 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8574 0.6052464575716868 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5626 0.3971444565311768 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2851 0.20125468282445524 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2033 0.14351131889937477 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1849 0.13052259156170387 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1577 0.11132186419297296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.4118181888772728E-4 0.0 0.0 0.0 0.0 7 1.4118181888772728E-4 0.0 0.0 7.059090944386364E-5 0.0 8 1.4118181888772728E-4 0.0 0.0 7.059090944386364E-5 0.0 9 1.4118181888772728E-4 0.0 0.0 7.059090944386364E-5 0.0 10-11 1.764772736096591E-4 0.0 0.0 1.0588636416579547E-4 0.0 12-13 2.117727283315909E-4 0.0 0.0 1.4118181888772728E-4 0.0 14-15 2.117727283315909E-4 0.0 0.0 1.4118181888772728E-4 0.0 16-17 4.235454566631818E-4 0.0 0.0 1.4118181888772728E-4 0.0 18-19 4.235454566631818E-4 0.0 0.0 1.4118181888772728E-4 0.0 20-21 4.235454566631818E-4 0.0 0.0 2.8236363777545457E-4 0.0 22-23 4.235454566631818E-4 0.0 0.0 6.000227302728409E-4 0.0 24-25 4.235454566631818E-4 0.0 0.0 9.88272732214091E-4 0.0 26-27 4.235454566631818E-4 0.0 0.0 0.0016588863719307954 0.0 28-29 4.235454566631818E-4 0.0 0.0 0.0031412954702519317 0.0 30-31 4.235454566631818E-4 0.0 0.0 0.006494363668835454 0.0 32-33 4.235454566631818E-4 0.0 0.0 0.009953318231584773 0.0 34-35 4.235454566631818E-4 0.0 0.0 0.012882840973505115 0.0 36-37 4.235454566631818E-4 0.0 0.0 0.017471250087356248 0.0 38-39 4.235454566631818E-4 0.0 0.0 0.02502447739784966 0.0 40-41 4.235454566631818E-4 0.0 0.0 0.03363656835000102 0.0 42-43 4.235454566631818E-4 0.0 0.0 0.04115450020577251 0.0 44-45 4.588409113851136E-4 0.0 0.0 0.04701354568961318 0.0 46-47 4.941363661070455E-4 0.0 0.0 0.05647272755509091 0.0 48-49 4.941363661070455E-4 0.0 0.0 0.06317886395225795 0.0 50-51 4.941363661070455E-4 0.0 0.0 0.06988500034942499 0.0 52-53 4.941363661070455E-4 0.0 0.0 0.08605031861206977 0.0 54-55 4.941363661070455E-4 0.0 0.0 0.11523965966710739 0.0 56-57 4.941363661070455E-4 0.0 0.0 0.15727654624092818 0.0 58-59 4.941363661070455E-4 0.0 0.0 0.21710234199460263 0.0 60-61 5.647272755509091E-4 0.0 0.0 0.28822268325929523 0.0 62-63 7.059090944386364E-4 0.0 0.0 0.32616529708537195 0.0 64-65 7.059090944386364E-4 0.0 0.0 0.37490832005635977 0.0 66-67 7.412045491605682E-4 0.0 0.0 0.4762415705630261 0.0 68-69 7.765000038825001E-4 0.0 0.0 0.6126937985180145 0.0 70-71 7.765000038825001E-4 0.0 0.0 0.7494989810202222 0.0 72-73 7.765000038825001E-4 0.0 0.0 0.9107992090994506 0.0 74-75 7.765000038825001E-4 0.0 0.0 1.0675816189742717 0.0 76-77 7.765000038825001E-4 0.0 0.0 1.2001866423645695 0.0 78-79 7.765000038825001E-4 0.0 0.0 1.3170851884036077 0.0 80-81 7.765000038825001E-4 0.0 0.0 1.432924870800988 0.0 82-83 7.765000038825001E-4 0.0 0.0 1.5951780761577086 0.0 84-85 7.765000038825001E-4 0.0 0.0 1.9065545777145911 0.0 86-87 8.117954586044319E-4 0.0 0.0 2.3200055343273003 0.0 88-89 8.470909133263636E-4 0.0 0.0 2.7697755138488773 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8345 0.0 34.119934 1 GTATCAA 13005 0.0 34.06538 1 TCAACGC 15780 0.0 27.982449 4 ATCAACG 15795 0.0 27.955877 3 CAACGCA 15910 0.0 27.756748 5 TATCAAC 15945 0.0 27.63522 2 AACGCAG 16195 0.0 27.261543 6 GTACATG 9390 0.0 25.058054 1 ACCGTCC 115 1.2775166E-4 24.795282 8 GAGTACT 8520 0.0 24.567045 12-13 ACGCAGA 17815 0.0 24.462406 7 CGCAGAG 17780 0.0 24.269999 8 TACATGG 9695 0.0 24.02459 2 GCAGAGT 18195 0.0 23.246288 9 ACATGGG 9540 0.0 23.117807 3 GTACTTT 9125 0.0 22.888569 14-15 AGTACTT 8955 0.0 21.861414 12-13 AGAGTAC 14150 0.0 21.694685 10-11 CATGGGA 6180 0.0 21.611996 4 ATGGGAT 2680 0.0 21.284798 5 >>END_MODULE