FastQCFastQC Report
Thu 26 May 2016
SRR937046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416613
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131070.9252350500807207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101590.7171330490402107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69120.4879243660759855No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37990.26817486497723797No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23480.1657474553741918No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17100.12071045514900683No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15790.11146304601186068No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14600.10306272778804092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA144250.032.4120981
GGTATCA96150.030.4904461
TCAACGC181300.025.6989334
ATCAACG181500.025.6715223
TATCAAC182350.025.5779042
CAACGCA182300.025.5579645
AACGCAG185250.025.150086
GTACATG97100.024.6136861
TACATGG100250.023.4993882
GAGTACT103150.023.25148612-13
AGTACTT107000.022.99188412-13
ACATGGG96700.022.7412193
ACGCAGA204700.022.6211857
CGCAGAG205000.022.495418
GTACTTT108000.022.05379914-15
AGAGTAC161750.021.94802710-11
TAAGGTG5200.021.9205685
CATGGGA63250.021.5508944
GCAGAGT211650.021.3622639
ATGGGAT25800.020.8018845
Produced by FastQC (version 0.11.5)