FastQCFastQC Report
Thu 26 May 2016
SRR937045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451850
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119960.8262561559389745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86130.5932431036264076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57000.3926025415848745No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29140.20070943968040775No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20630.14209456899817474No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18460.1271481213623997No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16030.11041085511588664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127400.033.0831641
GGTATCA82350.030.5242441
ATCAACG156400.026.8558243
TATCAAC157350.026.6936782
TCAACGC157200.026.6889254
CAACGCA158050.026.6055165
GTACATG94200.026.5835251
AACGCAG160900.026.1628866
TACATGG97450.025.6951852
ACATGGG94700.025.0364933
GAGTACT82650.023.59897812-13
AGTACTT86700.023.2912512-13
ACGCAGA178800.023.2513687
CGCAGAG179400.023.0676698
CATGGGA60800.022.7411464
AGAGTAC138000.022.22491310-11
GCAGAGT184200.022.1062019
GTACTTT89100.021.88573514-15
CATGGGG32850.021.2620374
GTATAGG5600.021.2131331
Produced by FastQC (version 0.11.5)