##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937045_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1451850 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9378214002824 33.0 31.0 34.0 30.0 34.0 2 32.04957950201467 33.0 31.0 34.0 30.0 34.0 3 32.037543134621345 34.0 31.0 34.0 30.0 34.0 4 35.62816475531219 37.0 35.0 37.0 33.0 37.0 5 35.630801391328305 37.0 35.0 37.0 33.0 37.0 6 35.74470158762958 37.0 35.0 37.0 35.0 37.0 7 35.6962482350105 37.0 35.0 37.0 33.0 37.0 8 35.66543237937803 37.0 35.0 37.0 33.0 37.0 9 37.28484416434205 39.0 37.0 39.0 34.0 39.0 10-11 37.17480628164066 39.0 37.0 39.0 33.5 39.0 12-13 37.07807314805248 39.0 37.0 39.0 33.0 39.0 14-15 38.23678616937011 40.0 38.0 41.0 33.0 41.0 16-17 38.176165581843854 40.0 38.0 41.0 33.0 41.0 18-19 38.177174983641564 40.0 38.0 41.0 33.5 41.0 20-21 38.09168612459965 40.0 38.0 41.0 33.0 41.0 22-23 37.96641044185005 40.0 38.0 41.0 33.0 41.0 24-25 38.067146055033234 40.0 38.0 41.0 33.0 41.0 26-27 38.01002789544374 40.0 38.0 41.0 33.0 41.0 28-29 37.91455384509419 40.0 38.0 41.0 33.0 41.0 30-31 37.68823397733925 40.0 37.0 41.0 33.0 41.0 32-33 37.439121465716156 40.0 36.5 41.0 32.5 41.0 34-35 37.231432310500395 40.0 36.0 41.0 32.0 41.0 36-37 37.026047456693185 39.0 36.0 41.0 31.0 41.0 38-39 36.85404862761304 39.0 35.5 41.0 31.0 41.0 40-41 36.642915246065364 39.0 35.0 41.0 30.5 41.0 42-43 36.36455660019975 39.0 35.0 41.0 30.0 41.0 44-45 36.015253641905154 39.0 35.0 41.0 29.5 41.0 46-47 35.533628818404104 39.0 35.0 40.0 27.5 41.0 48-49 35.16688535317009 38.5 35.0 40.0 26.0 41.0 50-51 34.40874401625513 38.0 34.0 39.5 25.0 40.5 52-53 34.58450287564142 38.0 34.5 39.5 25.0 40.5 54-55 34.974675758515 39.0 35.0 40.0 26.0 41.0 56-57 34.85790164273169 39.0 35.0 41.0 25.5 41.0 58-59 34.61108688914144 38.0 34.5 40.0 24.5 41.0 60-61 34.32016737266247 38.0 34.0 40.0 23.0 41.0 62-63 33.993408409959706 37.5 34.0 40.0 22.5 41.0 64-65 33.663678754692285 37.0 34.0 40.0 22.0 41.0 66-67 33.28238109997589 36.5 33.0 39.5 21.5 41.0 68-69 32.88637600303062 36.0 33.0 39.0 20.0 41.0 70-71 32.48387712229225 35.5 32.5 39.0 18.5 40.5 72-73 32.08146640493164 35.0 32.0 38.0 17.0 40.0 74-75 31.651635843923273 35.0 32.0 37.0 12.5 39.5 76-77 31.237580672934534 35.0 31.0 37.0 9.0 39.0 78-79 30.821726418018393 35.0 31.0 36.0 7.0 39.0 80-81 30.398605572200985 35.0 31.0 36.0 3.5 37.5 82-83 29.995608361745358 34.0 30.5 35.0 2.0 37.0 84-85 29.61603471432999 34.0 30.0 35.0 2.0 37.0 86-87 29.296150084375107 34.0 30.0 35.0 2.0 36.0 88-89 28.951566277508007 34.0 29.0 35.0 2.0 36.0 90-91 28.589347384371663 34.0 29.0 35.0 2.0 35.5 92-93 28.241586252023282 34.0 29.0 35.0 2.0 35.0 94-95 27.867091641698522 34.0 28.5 35.0 2.0 35.0 96-97 27.389027103350898 34.0 27.0 35.0 2.0 35.0 98-99 26.796511347591004 34.0 26.0 35.0 2.0 35.0 100-101 24.29703378448187 31.0 13.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21532786902538348 1101 2 0.2513886809387671 1101 3 0.19837928541555527 1101 4 0.1102562227442192 1101 5 0.1893415449435878 1101 6 0.04408498348438883 1101 7 -0.02225239099388432 1101 8 -0.09823780464147802 1101 9 0.13291654340797265 1101 10-11 0.16397230638726512 1101 12-13 0.2774268403165365 1101 14-15 0.24380312950293614 1101 16-17 0.3458278811665778 1101 18-19 0.3724760057707712 1101 20-21 0.24483359025029472 1101 22-23 0.4147241134207249 1101 24-25 0.29837180446530454 1101 26-27 0.38103881521253413 1101 28-29 0.33561423661966927 1101 30-31 0.3511859515611562 1101 32-33 0.3641609401751964 1101 34-35 0.37036585465303773 1101 36-37 0.29276016130460647 1101 38-39 0.2601762423091998 1101 40-41 0.31022862942815266 1101 42-43 0.28216046780236326 1101 44-45 0.24361689687406596 1101 46-47 0.2752002921680514 1101 48-49 0.33678418760303686 1101 50-51 0.3927548681461914 1101 52-53 0.35192756963918725 1101 54-55 0.2770073902384169 1101 56-57 0.3156432740434738 1101 58-59 0.284445448720966 1101 60-61 0.5092026454660044 1101 62-63 0.4487012899899696 1101 64-65 0.46949739892439624 1101 66-67 0.5226881080783627 1101 68-69 0.4838172497013531 1101 70-71 0.4654553834831887 1101 72-73 0.34032007574555934 1101 74-75 0.3722341432784795 1101 76-77 0.3951790053711264 1101 78-79 0.39384585927053806 1101 80-81 0.42494024147093157 1101 82-83 0.42931620951112137 1101 84-85 0.6009277108164639 1101 86-87 0.5927284277228431 1101 88-89 0.5746279102917526 1101 90-91 0.4979430614455893 1101 92-93 0.7253214926963736 1101 94-95 0.7827771914961694 1101 96-97 0.6760566047669094 1101 98-99 0.4929938156902267 1101 100-101 0.8831963029595364 1102 1 0.25789432078935803 1102 2 0.34717508314165 1102 3 0.27962789874658966 1102 4 0.24396992112536253 1102 5 0.3754879862416445 1102 6 0.2799196142762952 1102 7 0.20593461898236853 1102 8 0.25576204399733626 1102 9 0.2500937090857249 1102 10-11 0.4126043958637169 1102 12-13 0.31843310757511034 1102 14-15 0.4700748383512874 1102 16-17 0.5451560056915525 1102 18-19 0.4906941030054597 1102 20-21 0.6318303385722288 1102 22-23 0.5393917171467919 1102 24-25 0.2732102716810516 1102 26-27 0.4029603130931534 1102 28-29 0.4142625424417119 1102 30-31 0.33705955462357196 1102 32-33 0.5872247945424576 1102 34-35 0.45377524590293916 1102 36-37 0.23886021751523145 1102 38-39 0.29041193237065954 1102 40-41 0.36373885051250454 1102 42-43 0.4684498613291552 1102 44-45 0.19253767223143115 1102 46-47 0.22269122181679535 1102 48-49 0.15872376473610927 1102 50-51 0.03226977495879879 1102 52-53 0.17575075260963047 1102 54-55 0.2501782970665474 1102 56-57 0.25015983353908666 1102 58-59 0.2068392064305229 1102 60-61 0.017837348186823476 1102 62-63 0.3427680286286616 1102 64-65 0.2017913746846105 1102 66-67 0.16267973793429746 1102 68-69 0.2545532466896603 1102 70-71 0.13047464969193356 1102 72-73 -0.04965813212509218 1102 74-75 0.1024921817539699 1102 76-77 0.2691832719453373 1102 78-79 0.09788995194756822 1102 80-81 0.03591742021490418 1102 82-83 0.14926212052755972 1102 84-85 0.04871068992797234 1102 86-87 0.017963280123048264 1102 88-89 -0.013182275022202816 1102 90-91 0.08714557559365588 1102 92-93 0.031595834194373396 1102 94-95 0.16782338576501488 1102 96-97 0.06792213782024348 1102 98-99 0.15165397166986594 1102 100-101 0.3018965890580496 1103 1 0.18744889976810342 1103 2 0.21332509026374424 1103 3 0.2720193923287937 1103 4 0.3120522138416604 1103 5 0.29796443497224345 1103 6 0.2443460747011983 1103 7 0.1689788656494926 1103 8 0.13370166622741664 1103 9 0.21966109024787528 1103 10-11 0.24676195768086018 1103 12-13 0.3758792126690764 1103 14-15 0.45141811799634723 1103 16-17 0.5488753213374764 1103 18-19 0.6110884383910076 1103 20-21 0.5674351801891646 1103 22-23 0.4863380974525313 1103 24-25 0.36456651177827126 1103 26-27 0.47427761907764676 1103 28-29 0.4737311463178955 1103 30-31 0.4947692651148543 1103 32-33 0.48964278934772665 1103 34-35 0.35212696478949823 1103 36-37 0.4842888004999324 1103 38-39 0.4696315970871012 1103 40-41 0.3842545252737395 1103 42-43 0.2642256683678923 1103 44-45 0.25368937634623734 1103 46-47 0.18989019904896054 1103 48-49 0.29160216333772127 1103 50-51 0.3278783446840947 1103 52-53 0.24874992785358074 1103 54-55 0.22579154451270966 1103 56-57 0.07973445989294703 1103 58-59 0.21736495763556007 1103 60-61 0.21698515889035974 1103 62-63 0.22079153329671186 1103 64-65 0.17837639226123514 1103 66-67 0.028580032138314948 1103 68-69 0.18104638046367683 1103 70-71 0.3027955554112367 1103 72-73 0.17265582735459617 1103 74-75 0.15288776570411144 1103 76-77 -0.1407378926313001 1103 78-79 -0.08226842231582054 1103 80-81 -0.123503703036004 1103 82-83 -0.11944111325473017 1103 84-85 0.01522752884379841 1103 86-87 0.039378273097675276 1103 88-89 0.044001807361958356 1103 90-91 0.006170358721501401 1103 92-93 0.17857773704197655 1103 94-95 0.3609726349556439 1103 96-97 0.44302822184688395 1103 98-99 0.5214362466248907 1103 100-101 0.649825818306816 1104 1 0.25534590156513204 1104 2 0.18777179413208955 1104 3 0.2718633344455057 1104 4 0.28649458276880324 1104 5 0.3561925474600258 1104 6 0.259626742439778 1104 7 0.22105136162982575 1104 8 0.2691606970921967 1104 9 0.2659007257975432 1104 10-11 0.332586699948898 1104 12-13 0.40708645438540003 1104 14-15 0.4188865517426805 1104 16-17 0.2728656741228761 1104 18-19 0.3440406909840945 1104 20-21 0.3884966890639703 1104 22-23 0.3335073808473439 1104 24-25 0.4029790169013836 1104 26-27 0.3236514171166718 1104 28-29 0.39129731328406336 1104 30-31 0.30583715817579105 1104 32-33 0.330535471966968 1104 34-35 0.28252317841540986 1104 36-37 0.24456044367909868 1104 38-39 0.26284524663469 1104 40-41 0.33202459211065616 1104 42-43 0.25298950077118576 1104 44-45 0.16343333895540724 1104 46-47 0.03281990994778994 1104 48-49 0.04892998735486742 1104 50-51 0.04128631782322145 1104 52-53 -0.049125753914445625 1104 54-55 -0.06793559852459197 1104 56-57 -0.07167183444168046 1104 58-59 -0.01853564610352265 1104 60-61 -0.08343119250785236 1104 62-63 0.008917144129334531 1104 64-65 0.09123914802275834 1104 66-67 -0.10332513511160357 1104 68-69 -0.051149424889580075 1104 70-71 -0.017769208610324938 1104 72-73 -0.03215044018221391 1104 74-75 -0.051650129563707736 1104 76-77 -0.05838937912204756 1104 78-79 0.14648406042323003 1104 80-81 0.13092069558942754 1104 82-83 0.050926410942125244 1104 84-85 0.16355128589792756 1104 86-87 0.09661506334957437 1104 88-89 0.10486792861420469 1104 90-91 -0.03111230309002977 1104 92-93 -0.13430373778383498 1104 94-95 0.08923940723724044 1104 96-97 0.023047098545390554 1104 98-99 0.006866115157947661 1104 100-101 0.023958830066330705 1105 1 0.2925341970884041 1105 2 0.2858910188968622 1105 3 0.20133338409318924 1105 4 0.32323369332983276 1105 5 0.29348157996308544 1105 6 0.17159615918497195 1105 7 0.02449909794857774 1105 8 0.19770073169086544 1105 9 0.28787474866641816 1105 10-11 0.16872648701236415 1105 12-13 0.46465684720688216 1105 14-15 0.2969354715890944 1105 16-17 0.38917908743632523 1105 18-19 0.3384720280960849 1105 20-21 0.3143416138892192 1105 22-23 0.29629007729324286 1105 24-25 0.015256358288979754 1105 26-27 0.10441663138933421 1105 28-29 0.020235812742576798 1105 30-31 -0.1464446311098726 1105 32-33 0.04953907247892175 1105 34-35 -0.20474229605049032 1105 36-37 0.06434541505784352 1105 38-39 0.1415806898774079 1105 40-41 0.028464779309146593 1105 42-43 0.11582898263500851 1105 44-45 -0.12856193888878664 1105 46-47 -0.25345331405750215 1105 48-49 -0.3144276834233253 1105 50-51 -0.09092085929101756 1105 52-53 -0.28277357532342506 1105 54-55 -0.08264223063946474 1105 56-57 -0.038726183185019636 1105 58-59 -0.029441347148512875 1105 60-61 -0.21990650579459015 1105 62-63 -0.08591230623000712 1105 64-65 0.02316301336924198 1105 66-67 0.08309980968523689 1105 68-69 0.26995585140752354 1105 70-71 0.26507887017900345 1105 72-73 0.06687067063197816 1105 74-75 0.15112215915234728 1105 76-77 0.11560694301125807 1105 78-79 0.30971312893140635 1105 80-81 0.3010756512159283 1105 82-83 0.24199527776537622 1105 84-85 0.448851861839195 1105 86-87 0.12742642676218452 1105 88-89 0.13355335497951515 1105 90-91 0.24997947128726494 1105 92-93 0.16317186030902064 1105 94-95 0.13595771991920103 1105 96-97 0.14033579701657928 1105 98-99 -0.19098975184814293 1105 100-101 -0.2535987992215887 1106 1 0.06982849108814904 1106 2 0.22067519986246253 1106 3 0.2552071492528327 1106 4 0.21776933589265468 1106 5 0.29518615750427557 1106 6 0.18544925739459472 1106 7 0.23443161827619718 1106 8 0.24464621394116648 1106 9 0.20855864043342365 1106 10-11 0.2991947826626955 1106 12-13 0.3560121968855299 1106 14-15 0.41768703127011264 1106 16-17 0.19357893842821028 1106 18-19 0.1850952208525527 1106 20-21 0.4189472427104022 1106 22-23 0.3808541641578458 1106 24-25 0.3626676890483651 1106 26-27 0.2572601698961421 1106 28-29 0.3875579070595663 1106 30-31 0.40988122903055313 1106 32-33 0.4008465097077618 1106 34-35 0.26677055243182934 1106 36-37 0.18055911290624493 1106 38-39 0.17698770296811972 1106 40-41 0.12493663569861013 1106 42-43 0.07698309209850862 1106 44-45 0.19493180603983262 1106 46-47 0.2519777047953937 1106 48-49 0.17363730155202717 1106 50-51 -0.030514118655297295 1106 52-53 0.23841500217417178 1106 54-55 0.1670419692493752 1106 56-57 0.3941555188364063 1106 58-59 0.27865695659620116 1106 60-61 0.2778340992140258 1106 62-63 0.1921812716589244 1106 64-65 0.18315676176547413 1106 66-67 0.17522377968150238 1106 68-69 0.02935975950200742 1106 70-71 -0.12845240418713644 1106 72-73 -0.16551783830603384 1106 74-75 0.1282423791770917 1106 76-77 0.19948716783121512 1106 78-79 0.18015953901482007 1106 80-81 -0.024956135860985995 1106 82-83 0.13318492526805414 1106 84-85 0.17062651480583213 1106 86-87 0.2370843619892291 1106 88-89 0.4056249343565561 1106 90-91 0.33359269020931137 1106 92-93 0.03226045375761544 1106 94-95 0.14860830084279186 1106 96-97 0.05573034652958242 1106 98-99 0.07524088774739468 1106 100-101 0.13114406765888376 1107 1 0.14955667865947575 1107 2 0.15746843567530533 1107 3 0.2399825408909706 1107 4 0.1698131163678056 1107 5 0.13653380767545542 1107 6 0.17242923457065018 1107 7 0.1392972682398863 1107 8 0.13768491090142732 1107 9 0.2761486393959771 1107 10-11 0.14928296608421476 1107 12-13 0.3666849845191038 1107 14-15 0.3067704424123505 1107 16-17 0.4212348155938912 1107 18-19 0.2941962686790873 1107 20-21 0.517647834058053 1107 22-23 0.565507655183886 1107 24-25 0.41137606128614834 1107 26-27 0.2737167525291895 1107 28-29 0.42084714868213524 1107 30-31 0.45931581768083163 1107 32-33 0.45688188682157715 1107 34-35 0.4003814041799387 1107 36-37 0.4101342763049374 1107 38-39 0.2973710414887165 1107 40-41 0.42721124924773335 1107 42-43 0.43376284103512575 1107 44-45 0.48718203946653915 1107 46-47 0.7071986820751945 1107 48-49 1.0133898692457635 1107 50-51 0.6597545814173245 1107 52-53 0.8650619572525002 1107 54-55 0.8556896505441145 1107 56-57 0.8765318026832674 1107 58-59 0.8681371931352615 1107 60-61 0.8697393798447948 1107 62-63 1.0391311627264699 1107 64-65 1.0661581104530953 1107 66-67 0.982581908118064 1107 68-69 1.0075992034277306 1107 70-71 0.9061466206079629 1107 72-73 0.9093472929316491 1107 74-75 0.8958920316306695 1107 76-77 0.7774905914228718 1107 78-79 0.7792232385687115 1107 80-81 0.739451479036827 1107 82-83 0.702408911158706 1107 84-85 0.5666608544676244 1107 86-87 0.471924594378482 1107 88-89 0.6045304224777297 1107 90-91 0.7409871294664967 1107 92-93 0.7859563765711854 1107 94-95 0.7265728199837831 1107 96-97 0.5770110810456082 1107 98-99 0.5052128765429131 1107 100-101 0.5505081095719468 1108 1 -0.17442886545378755 1108 2 0.06241308576916538 1108 3 -0.10468538891142742 1108 4 0.12187389892717704 1108 5 0.13819605921489853 1108 6 0.07562683929103287 1108 7 0.03741016684400478 1108 8 0.06871536065962403 1108 9 0.1641593760497173 1108 10-11 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-1.96054323483407 2316 94-95 -1.542060500396392 2316 96-97 -1.6182790292181508 2316 98-99 -1.602334766791877 2316 100-101 -1.6060915245013376 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2170.0 3 755.0 4 787.0 5 786.0 6 946.0 7 1203.0 8 1442.0 9 1909.0 10 2506.0 11 3398.0 12 4961.0 13 6623.0 14 7665.0 15 10090.0 16 13742.0 17 19311.0 18 16820.0 19 8821.0 20 8117.0 21 8408.0 22 8834.0 23 9772.0 24 10827.0 25 12642.0 26 14321.0 27 17096.0 28 20583.0 29 24821.0 30 30812.0 31 38877.0 32 49469.0 33 66371.0 34 92938.0 35 134843.0 36 202594.0 37 294126.0 38 258530.0 39 43928.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.33098358667974 19.22647130149142 15.116045150409507 21.32649996141933 2 15.1866942663971 21.106905328561933 40.67341505333384 23.03298535170713 3 18.42961070916607 27.17451578882834 30.743319342752695 23.652554159252897 4 12.144683260802022 17.372517923856932 38.65325302416646 31.829545791174578 5 12.753057083944382 38.360063291837115 34.011712849125054 14.875166775093449 6 29.208690001977565 37.503591664444485 18.15559676064597 15.132121572931975 7 26.496013821599142 34.51300975929999 21.753312285888867 17.237664133212 8 26.187101322144457 33.52866552784138 20.571002127200675 19.71323102281349 9 27.294592464394373 16.262428898981437 21.200091494333964 35.24288714229022 10-11 24.253622477011287 27.197865696654045 27.493175292924178 21.055336533410493 12-13 26.081203332853324 25.161795518575907 28.06832721382364 20.688673934747126 14-15 23.23834966989885 25.50285012990833 27.649997364977487 23.608802835215336 16-17 20.915930934902892 29.067397989550958 27.948334821330157 22.068336254215993 18-19 20.945008275194567 26.90335502737445 31.47133131605732 20.680305381373664 20-21 22.3092417339118 25.71997183901949 31.425394756601783 20.545391670466927 22-23 21.89171689340451 25.713965903220252 31.497503013604273 20.896814189770964 24-25 21.550076093855633 26.032054127717142 31.523623190252547 20.89424658817468 26-27 21.20739057065124 26.072838103109824 31.69928022867376 21.020491097565174 28-29 20.998858671581885 26.2345047619638 31.735473547974713 21.0311630184796 30-31 21.294865172021904 25.99965561180563 31.512036367393325 21.193442848779142 32-33 21.060389408228435 26.22719796341756 31.44332311184948 21.26908951650453 34-35 21.546883695813886 26.136861434530335 31.283267945404752 21.032986924251023 36-37 21.498915177187726 25.93249991390295 31.224609980369873 21.34397492853945 38-39 21.26276304406522 26.362436012585338 31.15694618169395 21.21785476165549 40-41 21.33406389574126 26.275416287983017 31.18146887814474 21.209050938130982 42-43 21.26300411613337 26.4271122045834 30.50412302114271 21.80576065814052 44-45 21.40891422211064 26.467052175209744 30.42104599756793 21.70298760511169 46-47 21.779557116782037 26.69979681096532 29.941901711609326 21.578744360643316 48-49 21.75487499715879 27.02736583124806 29.372378769939257 21.845380401653895 50-51 21.593885312413796 27.298337257640863 29.25425758954877 21.853519840396572 52-53 21.83895537129971 27.453807084222433 28.767858299020695 21.939379245457165 54-55 21.575640272829496 27.37273323701761 28.8805966294089 22.171029860743996 56-57 21.749095287228222 27.513393330679232 28.566985939241324 22.17052544285122 58-59 22.02317137387216 27.077673109434997 28.874985406394458 22.024170110298385 60-61 21.940782517714204 27.2062080133572 29.287553872741512 21.565455596187082 62-63 21.631060832526302 27.292911940995523 29.49259619980962 21.58343102666856 64-65 21.572003855827322 27.39483348067627 29.18244131452165 21.85072134897476 66-67 21.639737356564915 27.502958596185717 28.74064374093306 22.11666030631631 68-69 21.49095056054619 27.39152193912574 29.17902173677182 21.93850576355625 70-71 21.832611956879315 27.363034638126223 28.834057006250397 21.970296398744065 72-73 21.720015444744224 27.503975712760827 28.59740435986805 22.178604482626902 74-75 21.4520257383802 28.08639944778423 28.35665175100828 22.10492306282729 76-77 21.51575049984636 27.575500569776622 28.585941192438437 22.32280773793858 78-79 21.417398939162165 27.63916908958703 28.63342469301734 22.310007278233464 80-81 21.636772690940163 28.0412612662275 28.164225076486314 22.157740966346022 82-83 21.76088318034217 27.739010370051904 28.325757841959888 22.174348607646046 84-85 21.472694819755432 27.73549525320489 28.14944912927775 22.642360797761935 86-87 21.466810546586405 27.783795736437977 28.29158898333544 22.457804733640174 88-89 21.528522367070074 27.564956793059608 28.134298516232782 22.772222323637532 90-91 21.463234169549818 27.39435688214845 28.41940755254082 22.72300139576091 92-93 21.633467315771853 27.53056632268501 28.36167541893052 22.474290942612615 94-95 21.44178022152885 27.200676179982636 28.545286527478943 22.812257071009576 96-97 21.286468128491812 27.268860093275745 28.599313865294395 22.84535791293805 98-99 21.088736263168446 27.586720046628237 28.206948626954127 23.11759506324919 100-101 21.132391643093403 27.403516713847644 28.360365500113172 23.10372614294578 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 734.0 1 550.0 2 455.0 3 541.0 4 497.0 5 559.0 6 876.5 7 1789.5 8 3260.0 9 4052.0 10 3550.0 11 3007.0 12 3080.5 13 3229.0 14 3362.0 15 3468.0 16 3557.5 17 3678.5 18 3810.0 19 4011.5 20 4294.5 21 4616.5 22 5130.0 23 6000.0 24 6837.5 25 7898.5 26 9507.0 27 11328.0 28 12955.5 29 15161.0 30 18265.0 31 21226.0 32 24440.5 33 28230.0 34 31519.5 35 34301.5 36 39247.5 37 43847.5 38 45227.0 39 45566.5 40 44929.5 41 44356.0 42 43853.0 43 45389.0 44 48127.0 45 49182.0 46 48984.0 47 50209.5 48 55776.5 49 55827.0 50 49832.0 51 45853.0 52 43668.0 53 43700.0 54 42986.5 55 39774.5 56 36465.5 57 33244.5 58 31080.5 59 28472.0 60 24274.0 61 20344.5 62 16538.0 63 12695.5 64 9350.0 65 6936.5 66 4958.0 67 3712.0 68 2639.0 69 1577.0 70 1093.5 71 779.5 72 556.0 73 442.0 74 320.0 75 216.0 76 151.5 77 73.5 78 37.0 79 25.5 80 20.5 81 20.0 82 18.5 83 11.0 84 8.5 85 11.0 86 8.0 87 5.0 88 5.0 89 3.0 90 1.0 91 0.5 92 1.5 93 1.5 94 1.5 95 2.5 96 1.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023831663050590625 2 0.009987257636808211 3 0.010951544581051761 4 0.02018114819024004 5 0.05468884526638426 6 0.03946688707511106 7 0.014395426524778732 8 0.044563832351826975 9 0.027137789716568515 10-11 0.02221303853703895 12-13 0.03313014429865344 14-15 0.01718497089919758 16-17 0.016117367496642217 18-19 0.015325274649585012 20-21 0.013500017219409719 22-23 0.006887763887453938 24-25 0.0016875021524262149 26-27 0.0 28-29 0.0022729620828597996 30-31 0.0 32-33 4.1326583324723625E-4 34-35 1.3775527774907877E-4 36-37 0.0 38-39 1.3775527774907877E-4 40-41 3.443881943726969E-5 42-43 1.3775527774907877E-4 44-45 1.0331645831180906E-4 46-47 0.0 48-49 2.0663291662361812E-4 50-51 1.0331645831180906E-4 52-53 2.7551055549815753E-4 54-55 0.0018941350690498328 56-57 5.510211109963151E-4 58-59 0.0010676034025553604 60-61 0.0033750043048524298 62-63 0.0019285738884871025 64-65 0.0014808692358025967 66-67 0.010193890553431827 68-69 0.012776802011227054 70-71 0.023590591314529737 72-73 0.015394152288459551 74-75 0.01677170506595034 76-77 0.027826566105313907 78-79 0.018011502565692047 80-81 0.04201535971346902 82-83 0.031614836243413574 84-85 0.01646175569101491 86-87 0.0121569032613562 88-89 0.011950270344732583 90-91 0.021489823328856288 92-93 0.02865309777180838 94-95 0.011399249233736268 96-97 0.016530633329889452 98-99 0.02541584874470503 100-101 0.03605744395082137 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1451850.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.46767418132938 #Duplication Level Percentage of deduplicated Percentage of total 1 71.17844250371192 33.07496674996971 2 14.655260090344838 13.619917018415673 3 5.581833674826026 7.781244856085647 4 2.7175670223060413 5.051160758337704 5 1.564128063553994 3.6340696617550323 6 0.9394747392637632 2.6193123651418753 7 0.6150964569457318 2.0007471226011053 8 0.4435900459574382 1.6490078180504928 9 0.31819383266298357 1.3307154608422764 >10 1.7637808693799284 15.166073407242067 >50 0.14778804263071058 4.722713715559097 >100 0.07052959051286734 5.7898915401842945 >500 0.0026783180092676225 0.8450508664526291 >1k 0.0011903635596744989 0.8960775019821692 >5k 2.975908899186247E-4 0.9896263337863263 >10k+ 1.4879544495931236E-4 0.8294248235939893 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11996 0.8262561559389745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8613 0.5932431036264076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5700 0.3926025415848745 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2914 0.20070943968040775 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2063 0.14209456899817474 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1846 0.1271481213623997 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1603 0.11041085511588664 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.3775527774907877E-4 0.0 0.0 0.0 0.0 7 1.3775527774907877E-4 0.0 0.0 0.0 0.0 8 1.3775527774907877E-4 0.0 0.0 0.0 0.0 9 1.3775527774907877E-4 0.0 0.0 0.0 0.0 10-11 1.3775527774907877E-4 0.0 0.0 0.0 0.0 12-13 1.3775527774907877E-4 0.0 0.0 0.0 0.0 14-15 1.3775527774907877E-4 0.0 0.0 6.887763887453938E-5 0.0 16-17 2.0663291662361815E-4 0.0 0.0 6.887763887453938E-5 0.0 18-19 2.0663291662361815E-4 0.0 0.0 6.887763887453938E-5 0.0 20-21 2.0663291662361815E-4 0.0 0.0 6.887763887453938E-5 0.0 22-23 2.0663291662361815E-4 0.0 0.0 3.443881943726969E-4 0.0 24-25 2.0663291662361815E-4 0.0 0.0 7.232152081826635E-4 0.0 26-27 2.0663291662361815E-4 0.0 0.0 0.0012742363191789786 0.0 28-29 2.0663291662361815E-4 0.0 0.0 0.002686227916107036 0.0 30-31 2.0663291662361815E-4 0.0 0.0 0.00561352756827496 0.0 32-33 2.0663291662361815E-4 0.0 0.0 0.008093122567758376 0.0 34-35 2.0663291662361815E-4 0.0 0.0 0.010917105761614492 0.0 36-37 2.0663291662361815E-4 0.0 0.0 0.014877569996900507 0.0 38-39 2.0663291662361815E-4 0.0 0.0 0.021558700967730826 0.0 40-41 2.0663291662361815E-4 0.0 0.0 0.02803319902193753 0.0 42-43 2.4107173606088784E-4 0.0 0.0 0.03505871818714054 0.0 44-45 3.443881943726969E-4 0.0 0.0 0.03970795881117195 0.0 46-47 4.132658332472363E-4 0.0 0.0 0.04711230499018494 0.0 48-49 4.132658332472363E-4 0.0 0.0 0.05341460894720529 0.0 50-51 4.132658332472363E-4 0.0 0.0 0.060336811654096496 0.0 52-53 4.132658332472363E-4 0.0 0.0 0.07617866859524056 0.0 54-55 4.132658332472363E-4 0.0 0.0 0.10579605331129249 0.0 56-57 4.132658332472363E-4 0.0 0.0 0.1485001894135069 0.0 58-59 4.132658332472363E-4 0.0 0.0 0.2083548575954816 0.0 60-61 4.132658332472363E-4 0.0 0.0 0.2799531632055653 0.0 62-63 4.821434721217757E-4 0.0 0.0 0.31900678444742914 0.0 64-65 4.821434721217757E-4 0.0 0.0 0.3666012329097359 0.0 66-67 5.165822915590454E-4 0.0 0.0 0.46148018045941386 0.0 68-69 5.510211109963151E-4 0.0 0.0 0.5942762682095257 0.0 70-71 5.854599304335848E-4 0.0 0.0 0.7283465922788167 0.0 72-73 6.198987498708544E-4 0.0 0.0 0.8870751110651927 0.0 74-75 6.198987498708544E-4 0.0 0.0 1.036264076867445 0.0 76-77 6.198987498708544E-4 0.0 0.0 1.1627234218410993 0.0 78-79 6.198987498708544E-4 0.0 0.0 1.2827082687605469 0.0 80-81 6.198987498708544E-4 0.0 0.0 1.403416330888177 0.0 82-83 6.198987498708544E-4 0.0 0.0 1.5667252126597102 0.0 84-85 6.198987498708544E-4 3.443881943726969E-5 0.0 1.8642766125977202 0.0 86-87 6.198987498708544E-4 6.887763887453938E-5 0.0 2.253607466336054 0.0 88-89 6.198987498708544E-4 6.887763887453938E-5 0.0 2.6904294520783827 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12740 0.0 33.083164 1 GGTATCA 8235 0.0 30.524244 1 ATCAACG 15640 0.0 26.855824 3 TATCAAC 15735 0.0 26.693678 2 TCAACGC 15720 0.0 26.688925 4 CAACGCA 15805 0.0 26.605516 5 GTACATG 9420 0.0 26.583525 1 AACGCAG 16090 0.0 26.162886 6 TACATGG 9745 0.0 25.695185 2 ACATGGG 9470 0.0 25.036493 3 GAGTACT 8265 0.0 23.598978 12-13 AGTACTT 8670 0.0 23.29125 12-13 ACGCAGA 17880 0.0 23.251368 7 CGCAGAG 17940 0.0 23.067669 8 CATGGGA 6080 0.0 22.741146 4 AGAGTAC 13800 0.0 22.224913 10-11 GCAGAGT 18420 0.0 22.106201 9 GTACTTT 8910 0.0 21.885735 14-15 CATGGGG 3285 0.0 21.262037 4 GTATAGG 560 0.0 21.213133 1 >>END_MODULE