FastQCFastQC Report
Thu 26 May 2016
SRR937045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1451850
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131700.9071185039776837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101590.6997279333264456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69650.4797327547611667No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37700.25966869855701347No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23520.16200020663291662No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16850.11605882150359885No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14910.10269655956193821No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.1002169645624548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA151900.030.4895951
GGTATCA103450.030.1675221
TAGGGTC2251.0186341E-1025.3296224
GTACATG100000.024.890641
TATCAAC187000.024.737872
ATCAACG186750.024.719273
TCAACGC187600.024.6325864
CAACGCA189450.024.3502945
AACGCAG193000.023.8777856
TACATGG104700.023.6339322
ACATGGG101300.023.6293133
GAGTACT103300.023.4476812-13
AGTACTT107200.022.7718512-13
GTACTTT109550.022.04568114-15
ACGCAGA214150.021.4086277
AGAGTAC164700.021.3460610-11
CGCAGAG215250.021.2109368
CATGGGG35400.020.9291384
CATGGGA64950.020.9130274
GTACCGT1150.003719473520.6562636
Produced by FastQC (version 0.11.5)