FastQCFastQC Report
Thu 26 May 2016
SRR937044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471175
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119790.814247115400955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87150.5923836389280677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57670.3919995921627271No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29500.20051999252298333No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21250.14444236749536934No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18840.12806090369942394No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT16270.11059187384233692No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132050.032.5047571
GCGTAAT300.0039558531.66027672-73
GGTATCA86650.030.0614221
TCAACGC159000.026.849484
TATCAAC160400.026.7040482
ATCAACG159800.026.6853163
CAACGCA160300.026.5715185
AACGCAG162350.026.2625986
GTACATG98200.025.7028081
TACATGG101900.024.1664162
GAGTACT85150.023.89060412-13
ACATGGG98700.023.4086193
ACGCAGA181600.023.3692347
CGCAGAG181350.023.1401828
GTACTTT89650.022.69179314-15
GGGACGT1050.002190193222.6302627
GCAGAGT187350.022.1439299
CATGGGA67350.021.81114
AGTACTT88500.021.58990912-13
ATAGCGT1353.7787406E-421.125896
Produced by FastQC (version 0.11.5)