FastQCFastQC Report
Thu 26 May 2016
SRR937044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471175
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133720.9089333355990961No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104050.7072578041361497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70400.47852906690230596No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT38550.26203544785630534No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23000.15633762128910567No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17190.11684537869390114No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.10732917565891209No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT15300.10399850459666593No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14730.10012405050384895No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104350.030.5933131
GTATCAA154350.030.4088521
TATCAAC189050.024.671522
TCAACGC189950.024.5546264
ATCAACG189950.024.5296233
CAACGCA191050.024.3635275
GTACATG98450.024.0305291
AACGCAG194650.023.964996
GAGTACT103950.023.90240912-13
ACATGGG97750.023.2258853
TACATGG102100.023.0736922
TAGACCG1252.257462E-422.7982965
GTACTTT110300.022.5232814-15
AGTACTT107750.022.06740612-13
ACGCAGA214300.021.5902167
CATGGGA64100.021.5623514
CGCAGAG215300.021.404598
AGAGTAC164450.021.36404810-11
GCAGAGT220200.020.4320479
ACTTTTT126850.019.64052416-17
Produced by FastQC (version 0.11.5)