FastQCFastQC Report
Thu 26 May 2016
SRR937043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118110
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141060.6659710779893395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95440.45059038482420644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63720.30083423429378076No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22710.10721822757080604No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21270.10041971380145506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA161000.032.4947431
GGTATCA105600.028.7083631
GTACATG120800.027.220171
ATCAACG192300.027.0339953
TCAACGC192850.026.9335274
CAACGCA192500.026.8831165
TATCAAC194050.026.8385372
AACGCAG197500.026.2025346
TACATGG127650.025.3505972
ACATGGG125550.024.7533053
ACGCAGA216750.023.7438877
GAGTACT97000.023.5604712-13
CGCAGAG216700.023.5520028
CATGGGG44500.022.8531744
GCAGAGT220500.022.3276949
GTACTTT105300.021.54519814-15
GTATAGG9500.021.5078261
CATGGGA80650.021.3892214
AGTACTT103750.021.0430212-13
TACGCTG1656.3048064E-520.160285
Produced by FastQC (version 0.11.5)