FastQCFastQC Report
Thu 26 May 2016
SRR937043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2118110
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146240.6904268427985327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108420.5118714325507174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73270.3459215999169071No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30730.1450821723139969No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24970.117888117236593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA169100.032.5648461
GGTATCA110650.030.615921
ATCAACG207800.026.5846273
TCAACGC208300.026.496764
CAACGCA209350.026.3411755
TATCAAC210100.026.339442
AACGCAG214650.025.7122976
GTACATG122650.023.3592531
ACGCAGA237250.023.1228527
CGCAGAG239050.022.8692678
GAGTACT118450.022.81617512-13
GCAGAGT248200.021.6625869
TACATGG129600.021.6248782
AGTACTT123950.021.47804512-13
ACATGGG123650.021.435643
GTACTTT125500.021.40276714-15
AGAGTAC208600.020.16229210-11
CATGGGA79200.019.6709164
CATTCCG4850.019.5863679
ACTTTTT143600.018.83759516-17
Produced by FastQC (version 0.11.5)