##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937043_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2118110 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.142107822539906 33.0 31.0 34.0 31.0 34.0 2 32.12820438976257 33.0 31.0 34.0 30.0 34.0 3 32.305077167852474 33.0 31.0 34.0 30.0 34.0 4 36.04228911624042 37.0 35.0 37.0 35.0 37.0 5 35.97743318335686 37.0 35.0 37.0 35.0 37.0 6 36.031665494237785 37.0 35.0 37.0 35.0 37.0 7 35.96879293332263 37.0 35.0 37.0 35.0 37.0 8 35.95526719575471 37.0 35.0 37.0 35.0 37.0 9 37.62627956055162 39.0 37.0 39.0 35.0 39.0 10-11 37.560550207496306 39.0 37.0 39.0 35.0 39.0 12-13 37.47020244463224 39.0 37.0 39.0 35.0 39.0 14-15 38.65349792975813 40.0 38.0 41.0 34.0 41.0 16-17 38.58301717096846 40.0 38.0 41.0 34.0 41.0 18-19 38.68324567657015 40.0 38.0 41.0 34.5 41.0 20-21 38.7736531624892 40.0 38.0 41.0 34.5 41.0 22-23 38.69992162824405 40.0 38.0 41.0 34.0 41.0 24-25 38.60208275302038 40.0 38.0 41.0 34.0 41.0 26-27 38.44200891360694 40.0 38.0 41.0 34.0 41.0 28-29 38.272429666070224 40.0 38.0 41.0 34.0 41.0 30-31 38.09731128222802 40.0 37.0 41.0 34.0 41.0 32-33 38.03572925863152 40.0 37.0 41.0 33.5 41.0 34-35 37.936985331262306 40.0 37.0 41.0 33.0 41.0 36-37 37.75744366439892 40.0 37.0 41.0 33.0 41.0 38-39 37.588843591692594 40.0 37.0 41.0 33.0 41.0 40-41 37.40258083857779 40.0 37.0 41.0 33.0 41.0 42-43 37.14776357224129 40.0 36.0 41.0 32.0 41.0 44-45 36.75820306782934 40.0 36.0 41.0 31.0 41.0 46-47 36.393818781838526 39.0 36.0 41.0 30.0 41.0 48-49 36.00009347956433 39.0 35.5 41.0 29.5 41.0 50-51 35.730600157687746 39.0 35.0 41.0 28.0 41.0 52-53 35.485148552247054 39.0 35.0 40.0 27.5 41.0 54-55 35.24876706120079 39.0 35.0 40.0 27.0 41.0 56-57 34.997239048019225 38.0 34.5 40.0 26.5 41.0 58-59 34.69063717181827 38.0 34.0 40.0 26.0 41.0 60-61 34.37647053269188 38.0 34.0 40.0 25.5 41.0 62-63 34.02928601441852 37.5 34.0 40.0 24.5 41.0 64-65 33.63904164561802 37.0 33.0 39.5 23.0 41.0 66-67 33.27162328679814 36.0 33.0 39.0 22.5 40.5 68-69 32.8458611686834 36.0 32.0 39.0 22.0 40.0 70-71 32.381873698721975 35.0 32.0 38.0 20.5 40.0 72-73 32.05847028718999 35.0 31.5 38.0 20.0 40.0 74-75 31.614984821373774 35.0 31.0 37.0 18.0 39.0 76-77 30.196964510813885 33.5 29.5 35.5 17.5 38.5 78-79 30.9077920882296 34.0 31.0 36.0 17.5 38.5 80-81 30.74325648809552 34.0 31.0 36.0 14.5 37.5 82-83 30.41793013582864 34.0 31.0 35.5 10.0 37.0 84-85 30.033173442361353 34.0 30.0 35.0 7.0 36.5 86-87 29.676523646080703 34.0 30.0 35.0 4.5 36.0 88-89 29.329439689156843 34.0 30.0 35.0 2.0 36.0 90-91 28.925518032585654 34.0 29.0 35.0 2.0 35.5 92-93 28.503283115607783 34.0 29.0 35.0 2.0 35.0 94-95 28.055787943024676 34.0 29.0 35.0 2.0 35.0 96-97 27.453621624939217 33.0 27.0 35.0 2.0 35.0 98-99 26.77013327919702 33.0 26.0 35.0 2.0 35.0 100-101 23.794094499341394 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24005757236531622 1101 2 0.1309841347349021 1101 3 0.18295927107548948 1101 4 0.19327507268593536 1101 5 0.17749302312430615 1101 6 0.13286984773247212 1101 7 0.27145168094406813 1101 8 0.2243158903428224 1101 9 0.29416363187102235 1101 10-11 0.33142712669612706 1101 12-13 0.2538605728936858 1101 14-15 0.4504225072971053 1101 16-17 0.4029657294195914 1101 18-19 0.35996941603116994 1101 20-21 0.38431952654342894 1101 22-23 0.3658425586914902 1101 24-25 0.3560286528361587 1101 26-27 0.30139797236542876 1101 28-29 0.27764586609641384 1101 30-31 0.33101369146213244 1101 32-33 0.3518372853653915 1101 34-35 0.41881114807171826 1101 36-37 0.39938430546245485 1101 38-39 0.2481982037911905 1101 40-41 0.2543764375611559 1101 42-43 0.30689435350473104 1101 44-45 0.46589084806421255 1101 46-47 0.39284847210391405 1101 48-49 0.3640875345264831 1101 50-51 0.36077043997251934 1101 52-53 0.137090207585139 1101 54-55 0.22397058585344354 1101 56-57 0.30435436761518275 1101 58-59 0.28668571166559786 1101 60-61 0.21834092569987718 1101 62-63 0.2348476644745432 1101 64-65 0.2550897080282297 1101 66-67 0.28053699236934193 1101 68-69 0.14963203136244374 1101 70-71 0.007405161287714179 1101 72-73 0.09571658782181203 1101 74-75 0.10452139285235518 1101 76-77 0.04394769119531716 1101 78-79 0.17707792274612544 1101 80-81 0.37716958052296334 1101 82-83 0.47026069511560564 1101 84-85 0.3505906557297891 1101 86-87 0.07617045374428244 1101 88-89 0.23584328485675954 1101 90-91 0.44922522848781554 1101 92-93 0.40223298837606336 1101 94-95 0.4508613828167576 1101 96-97 0.34964248455663594 1101 98-99 0.442249146625322 1101 100-101 0.7074622830333901 1102 1 0.3225406897064076 1102 2 0.18024676366904657 1102 3 0.2250664737555539 1102 4 0.18698008899554708 1102 5 0.21388939092823733 1102 6 0.2504553189482053 1102 7 0.22741095097845232 1102 8 0.27857684423509 1102 9 0.345687919978225 1102 10-11 0.3427095034923866 1102 12-13 0.3786754366863363 1102 14-15 0.46935850306542193 1102 16-17 0.45875714262597 1102 18-19 0.37498828229229986 1102 20-21 0.3061493775528632 1102 22-23 0.357429969429397 1102 24-25 0.32877748471484836 1102 26-27 0.2776419113586357 1102 28-29 0.3158394656732426 1102 30-31 0.40235575264523504 1102 32-33 0.566418701216584 1102 34-35 0.597773062909063 1102 36-37 0.6333989400388376 1102 38-39 0.6791499802171899 1102 40-41 0.729853471855435 1102 42-43 0.73514719666791 1102 44-45 0.9754151361714136 1102 46-47 0.8542741972207253 1102 48-49 0.9144280969879119 1102 50-51 0.9134865644545727 1102 52-53 0.9486526690150967 1102 54-55 0.8283760341475386 1102 56-57 0.8393696193122651 1102 58-59 0.829330867268169 1102 60-61 0.8547472555949724 1102 62-63 0.918661558506102 1102 64-65 0.8655621579973811 1102 66-67 0.8177329722688427 1102 68-69 0.6481927295904271 1102 70-71 0.7148389971336044 1102 72-73 0.8571348193302271 1102 74-75 0.7548554314664777 1102 76-77 0.6788365213108101 1102 78-79 0.734108514300388 1102 80-81 0.6693177776489456 1102 82-83 0.6948453728904447 1102 84-85 0.7269313063513181 1102 86-87 0.7222844447519492 1102 88-89 0.813440128725226 1102 90-91 0.9092151782365612 1102 92-93 0.8603702536498012 1102 94-95 1.050637191685663 1102 96-97 1.2090251875452616 1102 98-99 1.3308762294076537 1102 100-101 1.3710134599707082 1103 1 -0.0175406351879559 1103 2 -0.1918074287009084 1103 3 -0.07726504329209405 1103 4 -0.00478546547896741 1103 5 0.12636382477025876 1103 6 0.11832276368998862 1103 7 0.2016286419712756 1103 8 0.1327547812664065 1103 9 0.15680792167863444 1103 10-11 0.22440478574345946 1103 12-13 0.2084698755198744 1103 14-15 0.1470251313357096 1103 16-17 0.21127621911086436 1103 18-19 0.20572411797225953 1103 20-21 0.1350962678235632 1103 22-23 0.189339208625789 1103 24-25 0.26729306027308297 1103 26-27 0.2654206364755538 1103 28-29 0.19387526480809214 1103 30-31 0.2233689061464048 1103 32-33 0.27792402082869216 1103 34-35 0.1638929069100783 1103 36-37 0.16918916144194185 1103 38-39 0.16144085195888636 1103 40-41 0.14885871776022697 1103 42-43 0.20128365052952546 1103 44-45 0.379146681056433 1103 46-47 0.27745299199964535 1103 48-49 0.3127005857792611 1103 50-51 4.2392899593579614E-4 1103 52-53 0.1621463723523533 1103 54-55 0.21810687212786917 1103 56-57 0.11736537651278667 1103 58-59 0.20410725980528355 1103 60-61 0.13848027119828998 1103 62-63 0.23747480539273624 1103 64-65 0.01699840527277985 1103 66-67 0.12088246924203361 1103 68-69 0.2963079475138528 1103 70-71 0.1916343876487403 1103 72-73 0.27104131798532904 1103 74-75 0.2419735332319597 1103 76-77 0.14926548424528008 1103 78-79 0.2528629153135036 1103 80-81 0.30873835920318626 1103 82-83 0.3312279269487668 1103 84-85 0.2461226375467973 1103 86-87 0.001170432200449767 1103 88-89 0.25947393741939706 1103 90-91 0.4371973938582663 1103 92-93 0.4691167594082266 1103 94-95 0.5619006141328668 1103 96-97 0.8942755284845028 1103 98-99 0.8356280379797809 1103 100-101 0.7704237842075266 1104 1 0.16043155422922695 1104 2 0.021515249896765454 1104 3 0.027599341220160056 1104 4 0.07048977537865397 1104 5 0.022771225205573842 1104 6 0.10479019660992606 1104 7 0.18198048871536088 1104 8 0.13380009625436173 1104 9 0.21414609570267373 1104 10-11 0.1913880856476453 1104 12-13 0.09045578736563442 1104 14-15 0.14496940501048527 1104 16-17 0.1668139031199516 1104 18-19 0.3023592265943975 1104 20-21 0.1329793479513981 1104 22-23 0.1952490516885632 1104 24-25 0.10347829403692543 1104 26-27 0.05801856813366868 1104 28-29 0.18612414201417948 1104 30-31 0.2682107656908812 1104 32-33 0.26403775145828945 1104 34-35 0.19480551803872714 1104 36-37 0.16471216258882748 1104 38-39 0.24325411186475776 1104 40-41 0.20172473816800363 1104 42-43 0.1300733839392052 1104 44-45 0.08532442982659205 1104 46-47 -0.0847307060035618 1104 48-49 -0.13082180809794153 1104 50-51 -0.1229847920356022 1104 52-53 -0.1523593874678184 1104 54-55 -0.11264290315184411 1104 56-57 -0.2212566524821895 1104 58-59 -0.19947566723101318 1104 60-61 -0.17112731306890083 1104 62-63 0.02515311683846022 1104 64-65 -0.20105205804851067 1104 66-67 -0.05730255169028453 1104 68-69 -0.12172051484305513 1104 70-71 -0.12489470719102513 1104 72-73 -0.15191681396411383 1104 74-75 -0.17136240695844052 1104 76-77 -0.1789898015940885 1104 78-79 -0.13310219216192465 1104 80-81 -0.013721395272508374 1104 82-83 0.01762616270830719 1104 84-85 -0.03221845384818778 1104 86-87 0.013428073901643245 1104 88-89 -0.10209280042439417 1104 90-91 0.14802926180653841 1104 92-93 0.3020748859740685 1104 94-95 0.144724554555836 1104 96-97 0.17583699758185034 1104 98-99 0.2986246052191994 1104 100-101 0.44685931572911386 1105 1 0.14885881762263153 1105 2 0.02726373391769954 1105 3 0.09407572209212134 1105 4 0.09956829187067484 1105 5 0.07391115718404251 1105 6 0.10551465753887612 1105 7 0.13718161576262844 1105 8 0.12004465773264883 1105 9 0.17915438972683972 1105 10-11 0.21940776683621266 1105 12-13 0.202683415582662 1105 14-15 0.2689479475150307 1105 16-17 0.2311859843081976 1105 18-19 0.2709239835961057 1105 20-21 0.18641097512792015 1105 22-23 0.2527092772438877 1105 24-25 0.21837372531403076 1105 26-27 0.31961277925325504 1105 28-29 0.2561034008760643 1105 30-31 0.30673594070816534 1105 32-33 0.30744979631003844 1105 34-35 0.39168253633906147 1105 36-37 0.22991587468124663 1105 38-39 0.25730492623501533 1105 40-41 0.08360428073655868 1105 42-43 0.18774521448342796 1105 44-45 0.28808678152076794 1105 46-47 0.39893914240257544 1105 48-49 0.4769306126469175 1105 50-51 0.4305853052317872 1105 52-53 0.6045879213705305 1105 54-55 0.4498498542647624 1105 56-57 0.5319576364156475 1105 58-59 0.5371645520197177 1105 60-61 0.5468890334924623 1105 62-63 0.3321944880955172 1105 64-65 0.4532259080476919 1105 66-67 0.3038604674155536 1105 68-69 0.2519787576976924 1105 70-71 0.11648834058531321 1105 72-73 0.19079402726144679 1105 74-75 0.24903613164017457 1105 76-77 0.1647650858835199 1105 78-79 0.17142990251069534 1105 80-81 0.4448815174818108 1105 82-83 0.3624819228614946 1105 84-85 0.489453482853051 1105 86-87 0.48887966670273997 1105 88-89 0.45255142767220846 1105 90-91 0.5355782783953948 1105 92-93 0.5862686922383062 1105 94-95 0.520556489344628 1105 96-97 0.7647056229942244 1105 98-99 0.8544576299408178 1105 100-101 0.6938406272302977 1106 1 0.3921894330663207 1106 2 0.26913876112958235 1106 3 0.279571690775704 1106 4 0.22928386339933127 1106 5 0.14662229169186958 1106 6 0.19766101193849295 1106 7 0.2011097447330954 1106 8 0.3134855060308652 1106 9 0.4073542100294816 1106 10-11 0.38964430239769143 1106 12-13 0.43011437656774376 1106 14-15 0.6096585041414642 1106 16-17 0.6208640727082155 1106 18-19 0.5306133921384557 1106 20-21 0.5088603808204581 1106 22-23 0.4632629351143507 1106 24-25 0.5315938506272104 1106 26-27 0.5778698533526878 1106 28-29 0.6370964465088989 1106 30-31 0.5891888295214116 1106 32-33 0.5978777451873256 1106 34-35 0.45998831475717594 1106 36-37 0.6278366890981957 1106 38-39 0.5970200227926625 1106 40-41 0.6659786014290674 1106 42-43 0.73272744265158 1106 44-45 0.6995546596273741 1106 46-47 0.4752892474899184 1106 48-49 0.4840997062834944 1106 50-51 0.5534588204526258 1106 52-53 0.5469214935087265 1106 54-55 0.6233344988479175 1106 56-57 0.6043862621266243 1106 58-59 0.6838331251672329 1106 60-61 0.7604745896241383 1106 62-63 0.7752890033678099 1106 64-65 0.7489225717721766 1106 66-67 0.8712029452601371 1106 68-69 0.9539923379103996 1106 70-71 0.7414908143926411 1106 72-73 0.9209827886557989 1106 74-75 0.8074605340672711 1106 76-77 0.7321347756087064 1106 78-79 0.8655827125579165 1106 80-81 0.7613527203854673 1106 82-83 1.0103997687096538 1106 84-85 0.9135684535433839 1106 86-87 0.727996893505356 1106 88-89 1.0668126300613139 1106 90-91 1.2207541364107293 1106 92-93 1.2854022659597426 1106 94-95 1.1629488391449456 1106 96-97 1.3544444681022192 1106 98-99 1.3891884005416983 1106 100-101 1.5073294080193023 1107 1 0.2073071956958401 1107 2 0.17085665446217035 1107 3 0.13528532638844126 1107 4 0.20455339950735407 1107 5 0.1951408690856482 1107 6 0.16211819421732088 1107 7 0.17322261482900103 1107 8 0.15763431011860973 1107 9 0.1635862422543326 1107 10-11 0.2512051598430389 1107 12-13 0.25000960632149827 1107 14-15 0.4040553375532454 1107 16-17 0.37989302582654716 1107 18-19 0.39822095463021157 1107 20-21 0.2598272769967096 1107 22-23 0.2912719108199937 1107 24-25 0.3590629489111379 1107 26-27 0.3149856533582991 1107 28-29 0.252012933692626 1107 30-31 0.3048665477659185 1107 32-33 0.22893159318734746 1107 34-35 0.2982621044291349 1107 36-37 0.25964028748446566 1107 38-39 0.3088322342822636 1107 40-41 0.267126201344702 1107 42-43 0.21561357513959933 1107 44-45 0.20270210473572803 1107 46-47 0.07107508667527185 1107 48-49 0.16205847351879044 1107 50-51 0.2195306738906453 1107 52-53 0.14168082532307125 1107 54-55 0.17849056848833555 1107 56-57 0.003434119396388269 1107 58-59 0.015152015708096656 1107 60-61 0.12104569344164418 1107 62-63 0.07366602040804082 1107 64-65 0.06241706572378547 1107 66-67 0.06890574540184957 1107 68-69 0.1222082462811116 1107 70-71 0.10873655381561065 1107 72-73 -0.017363521476184474 1107 74-75 0.0858496188029676 1107 76-77 0.1282622038233221 1107 78-79 0.14367880632333652 1107 80-81 0.18148164160725955 1107 82-83 0.25454098827937344 1107 84-85 0.32995979181600177 1107 86-87 0.41167054312347773 1107 88-89 0.3442635203071056 1107 90-91 0.48456341383473855 1107 92-93 0.5941681246154964 1107 94-95 0.659587754787438 1107 96-97 0.8398021924143428 1107 98-99 0.800793440401975 1107 100-101 0.5924917270947816 1108 1 0.08059613264970267 1108 2 -0.0435834568519482 1108 3 -0.011445982207817451 1108 4 0.1312126652602359 1108 5 0.1549706571719014 1108 6 0.09076537454199496 1108 7 0.09473537799156873 1108 8 0.11658882408431026 1108 9 0.1893606299948445 1108 10-11 0.1412487915524494 1108 12-13 0.13706635940499012 1108 14-15 0.023331463552665355 1108 16-17 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-0.8583125774682188 2316 64-65 -0.9611740818065542 2316 66-67 -0.9991645513699368 2316 68-69 -0.9934675443349477 2316 70-71 -1.0086634493469333 2316 72-73 -0.7979854073032264 2316 74-75 -0.9514688572153531 2316 76-77 -1.240701491796468 2316 78-79 -0.9383439423798094 2316 80-81 -1.1041256257603287 2316 82-83 -1.124433626451605 2316 84-85 -1.1041581029593885 2316 86-87 -1.0737691508417981 2316 88-89 -1.2253638109272948 2316 90-91 -1.3129171385790777 2316 92-93 -1.6251738907428326 2316 94-95 -1.331180415504086 2316 96-97 -1.2971398572326613 2316 98-99 -1.2881171077756903 2316 100-101 -1.2214395857036315 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 13.0 5 33.0 6 90.0 7 246.0 8 560.0 9 1196.0 10 2038.0 11 3427.0 12 5677.0 13 7812.0 14 9640.0 15 13854.0 16 19212.0 17 26892.0 18 23839.0 19 12897.0 20 10627.0 21 10427.0 22 10543.0 23 11438.0 24 13331.0 25 15865.0 26 19416.0 27 24210.0 28 30053.0 29 38263.0 30 47904.0 31 60009.0 32 76673.0 33 100867.0 34 138509.0 35 196853.0 36 301093.0 37 469084.0 38 383054.0 39 32457.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.335537219741106 20.767135681144445 15.484655114285092 21.412671984829355 2 14.963164196467691 22.046950777319495 40.39318277961595 22.596702246596866 3 18.375315384731604 27.38715081055765 30.747367387330222 23.490166417380532 4 12.365764508480689 18.348373499720978 38.779692801021284 30.506169190777044 5 12.614904351422476 39.0357524269782 34.143742451840495 14.205600769758828 6 29.210733178950054 38.69902578011949 17.938865164852544 14.15137587607791 7 26.976502636784684 34.596739546104786 21.553790879604932 16.872966937505606 8 26.129237858279314 34.768826925891474 20.162456151946785 18.939479063882423 9 27.01724650749961 16.891521214667794 21.225384895024337 34.865847382808255 10-11 23.969197982350284 27.929572052038893 27.75835793075707 20.342872034853745 12-13 25.60974256717089 25.643405026187555 28.31808332662523 20.428769080016327 14-15 22.69025215876418 26.423155549050804 27.51535567085751 23.371236621327505 16-17 20.628484828455555 29.969831595148506 27.887668723531828 21.51401485286411 18-19 20.499383884689653 27.858326526950915 31.391499969312264 20.25078961904717 20-21 21.7085199136661 26.832016441495515 31.34169524830676 20.11776839653163 22-23 21.40693826099683 26.99472643063864 31.155416857481434 20.4429184508831 24-25 21.10122561945505 27.17571178136675 31.512026972220898 20.2110356269573 26-27 21.124619702031467 27.153440197613303 31.439310959568967 20.28262914078626 28-29 20.567206621762082 27.101624067271423 31.822743268384336 20.508426042582162 30-31 20.888088891050476 27.11296976296208 31.603254062561163 20.39568728342628 32-33 20.867166833562077 27.330905677890442 31.33965316914791 20.462274319399572 34-35 21.142834615482673 27.064391060112612 31.295224210101896 20.49755011430282 36-37 21.3890062676314 26.760330968709056 31.13150222495013 20.71916053870941 38-39 20.923776984964267 27.23596111431505 31.278444440405096 20.561817460315588 40-41 21.047177125029155 27.2265627582013 31.282005157652105 20.44425495911744 42-43 21.062895423107005 27.43097645561618 30.647853570018086 20.85827455125873 44-45 21.15066658986806 27.393212954665845 30.384746852594834 21.07137360287126 46-47 21.555097966129622 27.754886503475156 29.75832873212818 20.931686798267034 48-49 21.561918260031245 27.98194331070149 29.373657704733517 21.08248072453375 50-51 21.518955369110248 27.977458173737208 29.226545212305137 21.277041244847403 52-53 21.66705538312492 28.29564672189273 28.931788287705572 21.105509607276772 54-55 21.53383354280313 28.124530817555428 28.93655284162856 21.405082798012888 56-57 21.567193034886472 28.478295868134584 28.622080459884074 21.332430637094866 58-59 21.74848979148618 28.008979809592155 28.8827407776364 21.359789621285262 60-61 21.60388313119708 28.183814196270262 29.142274540982665 21.070028131549993 62-63 21.695568218836605 28.066389375433758 29.29864360207921 20.939398803650423 64-65 21.59430567011015 28.08455852324741 29.078417662563787 21.24271814407865 66-67 21.535317587527363 28.438172917446646 28.563851057712323 21.46265843731366 68-69 21.474215220172702 28.18451355217623 29.009872008535915 21.331399219115156 70-71 21.602633482955895 28.13622538043985 28.976244123586742 21.284897013017517 72-73 21.68735533961331 28.162050320864264 28.690682602275853 21.459911737246575 74-75 21.63183860217823 28.617949473949594 28.356818660914957 21.39339326295722 76-77 21.53749840953894 28.444997666536914 28.586421817891676 21.431082106032466 78-79 21.550621168156834 28.291454724173388 28.450134223688522 21.707789883981256 80-81 21.563572727137522 28.75183162570459 28.197345084066516 21.487250563091376 82-83 21.724129139720066 28.723824146230687 28.24808623667306 21.303960477376187 84-85 21.478538505574146 28.598572971441723 28.11680624162698 21.806082281357153 86-87 21.451929952284402 28.483754435935914 28.246887910482677 21.817427701297007 88-89 21.625040691170362 28.293624180481597 28.22037417227845 21.86096095606959 90-91 21.462041341631938 28.322081934482064 28.486049099502527 21.72982762438347 92-93 21.41768402436229 28.447939070194433 28.338667099127786 21.795709806315493 94-95 21.187707600022758 28.185753731341524 28.378614757385783 22.247923911249938 96-97 21.54353762565649 28.259325916539353 28.090965574417538 22.106170883386618 98-99 21.22889690253412 28.289835858764693 28.072702584542224 22.40856465415896 100-101 20.850679903724764 28.70729533250177 28.045827994413848 22.396196769359623 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1028.0 1 709.0 2 456.0 3 542.5 4 505.0 5 508.0 6 843.5 7 1762.0 8 2997.0 9 3694.5 10 3403.0 11 3086.5 12 3241.0 13 3343.5 14 3529.5 15 3813.5 16 4064.5 17 4307.0 18 4622.5 19 4964.0 20 5379.5 21 6021.0 22 6958.0 23 8110.0 24 9396.5 25 11695.5 26 15088.5 27 18236.5 28 20415.0 29 23739.0 30 28895.5 31 34194.5 32 40962.5 33 48568.0 34 56782.0 35 63318.0 36 70797.0 37 77633.5 38 77834.0 39 76639.5 40 75313.5 41 73567.0 42 71895.0 43 73404.5 44 76823.0 45 77920.0 46 75232.5 47 73267.0 48 79071.0 49 76119.0 50 64691.5 51 58184.5 52 54595.0 53 53310.5 54 51043.5 55 47837.0 56 44061.0 57 39649.0 58 36816.5 59 34057.5 60 29490.5 61 24089.0 62 19566.5 63 15641.5 64 11692.5 65 8665.0 66 6244.5 67 4797.5 68 3232.5 69 1805.0 70 1282.0 71 939.0 72 683.5 73 512.0 74 373.5 75 264.5 76 180.5 77 83.5 78 44.0 79 26.5 80 19.0 81 16.5 82 11.0 83 7.0 84 5.0 85 4.0 86 1.0 87 0.5 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015863198795152283 2 0.0029271378729150044 3 0.0029271378729150044 4 1.8884760470419383E-4 5 0.002077323651746132 6 2.3605950588024232E-4 7 0.0 8 0.0 9 0.0 10-11 9.442380235209691E-5 12-13 0.0011094796776371387 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002573048614094641 22-23 0.0 24-25 0.0037297401929078282 26-27 0.0016052046399856476 28-29 0.002997955724679077 30-31 0.015414685733979822 32-33 8.026023199928238E-4 34-35 0.00245501886115452 36-37 0.004013011599964119 38-39 0.0026202605152706895 40-41 0.004343494908196458 42-43 0.0021953534046862534 44-45 7.553904188167753E-4 46-47 2.596654564682665E-4 48-49 1.416357035281454E-4 50-51 1.416357035281454E-4 52-53 0.0013219332329293568 54-55 0.003021561675267101 56-57 5.19330912936533E-4 58-59 0.0011802975294012113 60-61 0.0012039034799892357 62-63 0.0 64-65 1.416357035281454E-4 66-67 4.7211901176048456E-5 68-69 0.0 70-71 1.1802975294012116E-4 72-73 1.1802975294012116E-4 74-75 6.845725670527026E-4 76-77 6.373606658766542E-4 78-79 1.8884760470419383E-4 80-81 0.005405762684657548 82-83 0.0015107808376335506 84-85 0.0034228628352635133 86-87 0.0018648700964539141 88-89 0.0010150558752850419 90-91 1.416357035281454E-4 92-93 2.3605950588024228E-5 94-95 2.1245355529221808E-4 96-97 0.0016052046399856476 98-99 0.0026438664658587135 100-101 7.553904188167753E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2118110.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.92195063220165 #Duplication Level Percentage of deduplicated Percentage of total 1 72.70192574115669 31.93210393269074 2 14.306377141265852 12.56727981048674 3 5.184248447068117 6.831069130715818 4 2.46661166777606 4.333535836034908 5 1.3247081284176205 2.909188250921749 6 0.8395849699049931 2.212572575982337 7 0.5410106174025178 1.6633569140335223 8 0.3715962490866813 1.305698568599722 9 0.27161888884575724 1.0737028283991135 >10 1.6685037411187562 13.979099899920316 >50 0.18841508206315813 5.78226409644864 >100 0.12560645859809855 10.456731677766603 >500 0.007748202695457081 2.3092343205896766 >1k 0.0017218228212126847 1.0941637728698712 >5k 1.0761392632579279E-4 0.3463189754986197 >10k+ 2.1522785265158559E-4 1.203679409041606 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14624 0.6904268427985327 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10842 0.5118714325507174 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7327 0.3459215999169071 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3073 0.1450821723139969 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2497 0.117888117236593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.7211901176048456E-5 0.0 8 0.0 0.0 0.0 4.7211901176048456E-5 0.0 9 0.0 0.0 0.0 4.7211901176048456E-5 0.0 10-11 0.0 0.0 0.0 7.081785176407268E-5 0.0 12-13 0.0 0.0 0.0 9.442380235209691E-5 0.0 14-15 0.0 0.0 0.0 1.1802975294012113E-4 0.0 16-17 0.0 0.0 0.0 1.4163570352814536E-4 0.0 18-19 0.0 0.0 0.0 2.596654564682665E-4 0.0 20-21 0.0 0.0 0.0 3.540892588203634E-4 0.0 22-23 0.0 0.0 0.0 6.1375471528863E-4 4.7211901176048456E-5 24-25 0.0 0.0 0.0 0.0011330856282251631 9.442380235209691E-5 26-27 0.0 0.0 0.0 0.0017468403435137929 9.442380235209691E-5 28-29 0.0 0.0 0.0 0.003588104489379683 9.442380235209691E-5 30-31 0.0 0.0 0.0 0.007081785176407269 9.442380235209691E-5 32-33 0.0 0.0 0.0 0.012251488355184574 9.442380235209691E-5 34-35 0.0 0.0 0.0 0.01647695351044091 9.442380235209691E-5 36-37 0.0 0.0 0.0 0.019238849729239747 9.442380235209691E-5 38-39 0.0 0.0 0.0 0.021835504293922413 9.442380235209691E-5 40-41 0.0 0.0 0.0 0.025258367129185923 9.442380235209691E-5 42-43 0.0 0.0 0.0 0.029294984679738068 9.442380235209691E-5 44-45 0.0 0.0 0.0 0.03444108190792736 9.442380235209691E-5 46-47 0.0 0.0 0.0 0.040838294517281916 9.442380235209691E-5 48-49 0.0 0.0 0.0 0.047943685644277215 9.442380235209691E-5 50-51 0.0 0.0 0.0 0.0581650622488917 9.442380235209691E-5 52-53 0.0 0.0 0.0 0.08002417249340213 1.1802975294012113E-4 54-55 0.0 0.0 0.0 0.10712380376845396 1.4163570352814536E-4 56-57 0.0 0.0 0.0 0.1406914655046244 1.4163570352814536E-4 58-59 0.0 0.0 0.0 0.1873604298171483 1.4163570352814536E-4 60-61 0.0 0.0 0.0 0.2529141546000916 1.4163570352814536E-4 62-63 0.0 0.0 0.0 0.29641992153382024 1.4163570352814536E-4 64-65 0.0 0.0 0.0 0.3384621195310914 1.4163570352814536E-4 66-67 0.0 0.0 0.0 0.42566250100325287 1.4163570352814536E-4 68-69 0.0 0.0 0.0 0.5366812866187309 1.4163570352814536E-4 70-71 0.0 0.0 0.0 0.6211905897238575 1.4163570352814536E-4 72-73 0.0 0.0 0.0 0.7014508217231399 1.4163570352814536E-4 74-75 0.0 0.0 0.0 0.8704458219828055 1.4163570352814536E-4 76-77 0.0 0.0 0.0 0.9799774327112378 1.4163570352814536E-4 78-79 0.0 0.0 0.0 1.0613943562893335 1.4163570352814536E-4 80-81 0.0 0.0 0.0 1.1735462275330366 1.652416541161696E-4 82-83 0.0 0.0 0.0 1.3843237603335048 1.8884760470419383E-4 84-85 0.0 0.0 0.0 1.5995864237456978 1.8884760470419383E-4 86-87 0.0 0.0 0.0 1.902781253098281 1.8884760470419383E-4 88-89 0.0 0.0 0.0 2.284560291958397 1.8884760470419383E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 16910 0.0 32.564846 1 GGTATCA 11065 0.0 30.61592 1 ATCAACG 20780 0.0 26.584627 3 TCAACGC 20830 0.0 26.49676 4 CAACGCA 20935 0.0 26.341175 5 TATCAAC 21010 0.0 26.33944 2 AACGCAG 21465 0.0 25.712297 6 GTACATG 12265 0.0 23.359253 1 ACGCAGA 23725 0.0 23.122852 7 CGCAGAG 23905 0.0 22.869267 8 GAGTACT 11845 0.0 22.816175 12-13 GCAGAGT 24820 0.0 21.662586 9 TACATGG 12960 0.0 21.624878 2 AGTACTT 12395 0.0 21.478045 12-13 ACATGGG 12365 0.0 21.43564 3 GTACTTT 12550 0.0 21.402767 14-15 AGAGTAC 20860 0.0 20.162292 10-11 CATGGGA 7920 0.0 19.670916 4 CATTCCG 485 0.0 19.586367 9 ACTTTTT 14360 0.0 18.837595 16-17 >>END_MODULE